Status
Please wait ...
Gene information
General information
TC006474 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 3169021..3174576
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006474
    iBeetle screen iB_01053
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006474 RA
      MYVFLDDDG SMRRCYCEG HCPDGQRNG TCELRPGGK 
      CFTTAQIGY DDNGDEYPL RYYGCLPPD EGGIMQCKG 
      HLVPHAEPK SIECCDGSD QCNLHLKPM YPERPTPDV 
      PQYGPFDSP PYIVLLTSV TFCITLCLI VIMICYLRY 
      RKKQVFVNK DYPEKYPAM KIHRHHLID EKSSGSGSG 
      MPTLVQRTI SKQIHMIRS VGKGRYGEV WLARWRSER 
      VAVKVFFTT EEQSWFRET EIYQTVLMR HDNILGFIA 
      ADIKGTGSW TQMLLITDY HENGSLFDY LQDHVLDTN 
      SLLTMVLSI ARGLAHLHT EIFGTRGKP AIAHRDIKS 
      KNILVKSNL ECCIADFGL AVKYFSDTN ELDDPRGAS 
      LVSTRSGTR RYMAPEILD NSINVRSFE AQKMADMYA 
      FGLVLWEMS RRCITGDKI TTADDYQVP YYDCVSSDP 
      GFEEMMQVV CVKNMRPHI PMRWESEYV LKTLSKVMQ 
      ECWHANPSV RLTALRVKK SLSKLDTDS CIKIV
    CDS sequence
    • >TC006474 RA
      atgtatgtc ttcctcgac gacgatggc tcgatgaga 
      agatgctac tgcgaagga cactgtccg gacggccaa 
      aggaatggg acctgcgag ctcaggccc ggcggaaag 
      tgcttcacc acggcccag atcggatat gacgacaac 
      ggtgacgaa tatcctctc aggtactac ggttgtctt 
      ccacccgac gagggaggc atcatgcaa tgcaaaggc 
      catctcgta ccgcacgcc gagcccaaa tccatcgaa 
      tgctgcgac ggctcggac cagtgcaac ctccacctc 
      aaacccatg tacccggaa cggcccacc cctgacgtc 
      ccccaatac ggccccttc gacagcccc ccgtacata 
      gtcctcctc acgtcggtc acgttctgc ataaccctc 
      tgcctcatc gtgataatg atttgctac ttgcggtat 
      cgcaagaag caagttttt gtaaataaa gactatccc 
      gagaaatac cccgcgatg aaaatccac cggcaccac 
      ctcatcgac gagaagagc agcggctcg ggctcgggg 
      atgccgacg ttagtgcag aggacgatt tcgaagcaa 
      attcacatg atacgctcg gtggggaag ggtcggtat 
      ggagaggtt tggttagcg aggtggcga agcgaacgg 
      gtggcagtt aaggtgttt ttcacaacg gaggagcag 
      tcctggttt agggaaacg gaaatttac caaactgtg 
      ttaatgagg cacgataat atactaggg ttcattgcg 
      gccgatatt aaggggacg gggtcctgg acgcagatg 
      ttgctgatt acggattat cacgaaaat ggctcgctg 
      tttgattat ctgcaagac catgtcttg gacacgaat 
      tcgctgctc accatggtc ttgagtatt gcgagaggg 
      ttggcacat ctgcatacg gagatattc ggcaccaga 
      ggcaagccg gcgatcgcc caccgcgac atcaagagc 
      aagaacatc ctggtgaag tcgaacctg gagtgctgc 
      atcgccgac ttcggcctc gccgtgaag tacttcagc 
      gacacgaac gagctggac gacccccgg ggggcgagc 
      ctggtgagc acccgcagc ggcacgcgc cgctacatg 
      gcccccgag atcctcgac aacagcatc aacgttcgc 
      agcttcgag gcgcagaaa atggccgac atgtacgcc 
      ttcggtttg gtgttgtgg gagatgtcg agacgttgc 
      ataaccggt gataagatc accacagcg gacgattac 
      caagtgcca tattatgac tgtgtgtcg agtgatccc 
      ggtttcgag gagatgatg caggtcgtt tgtgtgaag 
      aacatgagg ccgcacatt cccatgcgc tgggagtcg 
      gagtacgtg ctgaagacg ctctccaaa gtgatgcag 
      gagtgctgg cacgccaac ccctccgtg cggctgacg 
      gccttgcgc gtgaagaag agcctgtcc aagctggac 
      acagactcg tgcatcaaa atcgtctag
    mRNA sequence
    • >TC006474 RA
      ggatttttt agtctgttg atggtgtcg gtgtcggcc 
      gcagtgctc attttcgag acctattct tttcgtttt 
      ctctatgtt tgccatgta tgtcttcct cgacgacga 
      tggctcgat gagaagatg ctactgcga aggacactg 
      tccggacgg ccaaaggaa tgggacctg cgagctcag 
      gcccggcgg aaagtgctt caccacggc ccagatcgg 
      atatgacga caacggtga cgaatatcc tctcaggta 
      ctacggttg tcttccacc cgacgaggg aggcatcat 
      gcaatgcaa aggccatct cgtaccgca cgccgagcc 
      caaatccat cgaatgctg cgacggctc ggaccagtg 
      caacctcca cctcaaacc catgtaccc ggaacggcc 
      cacccctga cgtccccca atacggccc cttcgacag 
      ccccccgta catagtcct cctcacgtc ggtcacgtt 
      ctgcataac cctctgcct catcgtgat aatgatttg 
      ctacttgcg gtatcgcaa gaagcaagt ttttgtaaa 
      taaagacta tcccgagaa ataccccgc gatgaaaat 
      ccaccggca ccacctcat cgacgagaa gagcagcgg 
      ctcgggctc ggggatgcc gacgttagt gcagaggac 
      gatttcgaa gcaaattca catgatacg ctcggtggg 
      gaagggtcg gtatggaga ggtttggtt agcgaggtg 
      gcgaagcga acgggtggc agttaaggt gtttttcac 
      aacggagga gcagtcctg gtttaggga aacggaaat 
      ttaccaaac tgtgttaat gaggcacga taatatact 
      agggttcat tgcggccga tattaaggg gacggggtc 
      ctggacgca gatgttgct gattacgga ttatcacga 
      aaatggctc gctgtttga ttatctgca agaccatgt 
      cttggacac gaattcgct gctcaccat ggtcttgag 
      tattgcgag agggttggc acatctgca tacggagat 
      attcggcac cagaggcaa gccggcgat cgcccaccg 
      cgacatcaa gagcaagaa catcctggt gaagtcgaa 
      cctggagtg ctgcatcgc cgacttcgg cctcgccgt 
      gaagtactt cagcgacac gaacgagct ggacgaccc 
      ccggggggc gagcctggt gagcacccg cagcggcac 
      gcgccgcta catggcccc cgagatcct cgacaacag 
      catcaacgt tcgcagctt cgaggcgca gaaaatggc 
      cgacatgta cgccttcgg tttggtgtt gtgggagat 
      gtcgagacg ttgcataac cggtgataa gatcaccac 
      agcggacga ttaccaagt gccatatta tgactgtgt 
      gtcgagtga tcccggttt cgaggagat gatgcaggt 
      cgtttgtgt gaagaacat gaggccgca cattcccat 
      gcgctggga gtcggagta cgtgctgaa gacgctctc 
      caaagtgat gcaggagtg ctggcacgc caacccctc 
      cgtgcggct gacggcctt gcgcgtgaa gaagagcct 
      gtccaagct ggacacaga ctcgtgcat caaaatcgt 
      ctaggtgtt caggatgta atctccaaa gtagggtcg 
      tatgttatt acgtaacaa ttgtaggac ataaattat 
      tttgataca cccacaatg tgacgtgcc actgataca 
      ctcgggttg cgaaaaaag ggatttttt cactttgag 
      tgtaactag gacgataat tgttgaaat taacataca 
      atcccaccg ataagtaac aaattgtac ataggagta 
      agtaagttt tgtaaatag tacctttaa gtgaagata 
      tctgtaaga attcaaagt acctataca ttcacatat 
      gagtcttta cgacaatat tcaaccagc aaacgcaga 
      tgcaacgat ttttcttgg acgaggttt gccataaca 
      ggtgattcg aattcacaa tttcctatc agtagtgcc 
      atttttgta aatccggtt gaatttgag tgaaaagtt 
      gttgtgtct ggattggtg ttcaaagac aatcgtcac 
      tttgggtat ttatagtat cttgatttc agacacttg 
      ttaaaattg cacgaaatt caaattaag ttcaaattt 
      gctcaaaac gatttgttt atattcaag tgcaatgga 
      attgttttt ttattttac tgtaaatac attttcttc 
      cttttccgt ttcgttttc ttccctgag aaatatagg 
      ccgtgtgac tgtagtatt gtgcattta tgtttgtaa 
      atatacgtt atcttttgt aataatgtg aagtatcta 
      aattgtttt ttggatgtg cagtaatat tgcaagact 
      gatatattt tgaatgtgt atacaaaat aacatgtaa 
      taaatagtt tatagaaag