| General information | |||
| TC034094
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 9934581..9947784 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034094 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01050 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 7 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (11 dpi)
- >TC034094 RA
MPIQAPQWT EFLSCPVCC NAFDVALRS PISLGCGHT VCKACLSNL HRKQCPFDQ TTISIDIES LPVNTALLQ LVGPVIKKD DDAVDHKVV SRDNLAFYQ DSRKCVEEL AMYLKPHPN GNVCGSGSI LSRPMQRKL VTLINCQLV EDEGRTRAM RAARSLGER TVTELILQH QNPQQLSAN LWAAVRARG CQFLGPAMQ EEVLKLVLL ALEDGSALS RKVLVMFVV QRLEPHFPQ ASKTSIGHV VQLLYRASC FKVSKREGD SSLMQLKEE FRTYEALRR EHDAQIVQI ATEAGLRIA PDQWSALLY GDTAHKSHM QSIIDKLQT PQSFAQSVQ ELVIALQRT GDPGKLSVL RTPLELLAA IDPSPESST PSWKECSEA LEAVRLVVA GLVEFIHQH GSRKLQDTA HPQHAKYKI SMCRDLTLR GNCPRGTNC TFAHSNEEL EKYRAKNRK TIIRNKEHR IDSQSPTEK IYPQTEDYY SMPNTATVS PVPIPTVVQ IQRMGYDVP FNHNLPPYA PQPIYAQTP PYPHDVYQT APPNLVLAN NDQFYPPQT DFTNIGHQS NSMVEKKWD NVIPKNQNV LQHHTKRSS TKSLAELHQ RKQEVLSQL EKVVGKTAA TAISHNATS LARQESHHD LDSPTSPYS FWSGHVNSG PTFVRSDSI LAADDDYVP YDSPAISKY GPISRMHKD NGQVSTFRD GNMSSSLLR RPLASASPV TSLYYNNNP PPFHQGPIP AGAIVGHPG GGIGSPQLI YHPRVQDPS KELLAESQR VKLQLCNLE KQLNDLKLA TQGVTTLSE RDRLTQELQ LIEQGIQEK QKEACLNKI VHSLNQAMS NYNNANVTN LENSEEAMY EADIANDMR QLEIRLQEE IEEQEKIWS SEEDSNQ
- >TC034094 RA
atgccaatt caggctcca cagtggacg gagttcctg tcttgtcca gtatgttgc aatgccttt gacgtcgcc ctccggagc cccatcagc ctgggctgc gggcacacc gtgtgcaag gcctgcctg tccaacctc caccgcaag caatgcccc ttcgaccag accaccatc agcatagac atcgagagt ctgcccgtc aacacggcg ctgctccag ctggtgggg cccgtcatc aagaaggac gatgacgcc gtcgaccac aaggtcgtc tccagagac aacctggcc ttctaccag gactcgcgg aagtgcgtc gaggagctg gccatgtac ttgaagcca catcccaat gggaacgtc tgcggcagc ggaagcatt ctctccagg cccatgcag aggaagcta gtcactttg atcaactgc cagctggtg gaagacgag ggccggacg agggccatg agggccgcc aggtcactg ggagagcgc actgtcact gagcttatt ttacaacac cagaacccc cagcagctc tctgccaat ttgtgggcc gctgtgcgg gccagaggt tgccagttt ttggggcca gccatgcag gaagaggtg ttaaaacta gtccttctg gctttggaa gacggctca gctttatcc cgcaaagtg ttggtcatg tttgtggtg cagcgtttg gagccccat tttcctcaa gcctctaag accagtata ggccacgtg gtccagctt ttatacagg gcgtcttgt ttcaaagtt tcgaagcgt gaaggagac tcgtctttg atgcaacta aaggaggaa tttcgcact tatgaagct cttcgtcgc gaacacgat gctcaaatt gttcaaatt gcaactgaa gccggtttg cgaatcgct ccagatcaa tggtcggct cttttgtat ggcgatacg gctcataaa tctcacatg cagagcatc atcgacaag ttgcaaact cctcaatct ttcgctcaa tcggtacag gaattagtt attgcgtta caaaggacg ggggatccc gggaagttg tcagttttg agaactcct ctggaatta ttggcggcg attgatcct agtccagaa tcatcaact ccatcatgg aaagaatgt agtgaagcc ctcgaggcc gttcgccta gtcgtagca ggcctagta gaatttatt catcaacat ggtagtcga aaattacaa gatacagca catccacaa catgccaaa tataaaatc agcatgtgc cgtgattta acattgcgg ggaaattgt cctcgaggt accaattgc acgtttgca cattccaat gaagagttg gagaaatat cgggcgaaa aaccgcaag actataatc cgcaacaag gaacatcga atcgacagc caatcaccg accgagaaa atctacccc caaaccgaa gactactat tcgatgccg aacacagcg accgttagc cctgtgccc atcccaacc gtcgtccaa atccaacgg atgggttat gacgttccc ttcaaccac aacctgcct ccctacgcc ccacaaccc atctacgcc caaacacct ccatatcca cacgacgtc taccaaact gccccacca aacctagtt ctagccaac aacgaccaa ttctaccca ccacagact gatttcaca aacattggc catcagagc aactcgatg gtggagaaa aaatgggat aatgtgata ccgaaaaac cagaatgtg cttcaacac cacacgaaa agatcgtca acgaagagt ttagctgaa ttgcatcaa cggaaacag gaggttttg tcgcaactg gagaaagtt gtaggaaag acggcggca acggcgatt tcacataat gcgacgagt cttgccagg caagagtcg caccatgat ctggatagt ccaacatca ccgtattcg ttctggtca gggcatgtc aatagtggg cctactttt gtgcggtcg gattctatt ctggccgct gatgatgac tatgtgccg tatgattcg ccggcgatt agtaagtac gggcccatc tcgaggatg cataaggat aatggacag gtttcaacg ttcagggat gggaatatg tcatcgtct ttattacgc cgccctctt gcatctgct agtccagtg acctcatta tactataat aataatcca ccgccattc catcaaggt ccaattcca gctggtgct attgttgga catcctgga ggtggcata ggatctcct caattgatt tatcatcca agagttcaa gacccatcc aaggaactt ttggccgaa tcgcaacga gtaaagttg caattgtgc aatttagaa aaacaatta aacgaccta aagttggct acacagggt gtaacaact ttaagcgaa cgcgataga ctgacacag gaactgcaa ttaattgaa caagggatt caagagaag cagaaagag gcctgtttg aataaaatc gtccattcc ttaaaccaa gcgatgagc aactacaac aacgccaac gtgaccaat ttggagaat tccgaagag gcgatgtat gaagcagat attgccaac gacatgcgc cagttagag atccgactg caggaagag atcgaggag caagagaag atatggagc agtgaagag gactccaac cagtaa
- >TC034094 RA
attgtgcga caacgaccc gatttgctg attaatttg caattatct gaaataaag tttatgcga taattagat aaatcactc tgtaaatat ttatctgag ttccccttc cagtcgttc gaaatttgc gttacttat gcaaatatg tttaaaaat acaagacat tattccgcc aagcacaag caaaccaaa aaactgctc ggttaagta aaaattttc caaaatcgc gttattatg gaaaacgct cggtatgtg taatgcgca gagtgcgat caccttccc attgacgtg acgtatgac gttcgagaa gatttcaca acattgttg tgatgtctg gtggtgctt catgatgtg atgttctgt tgtagatta cctaagaat gccaattca ggctccaca gtggacgga gttcctgtc ttgtccagt atgttgcaa tgcctttga cgtcgccct ccggagccc catcagcct gggctgcgg gcacaccgt gtgcaaggc ctgcctgtc caacctcca ccgcaagca atgcccctt cgaccagac caccatcag catagacat cgagagtct gcccgtcaa cacggcgct gctccagct ggtggggcc cgtcatcaa gaaggacga tgacgccgt cgaccacaa ggtcgtctc cagagacaa cctggcctt ctaccagga ctcgcggaa gtgcgtcga ggagctggc catgtactt gaagccaca tcccaatgg gaacgtctg cggcagcgg aagcattct ctccaggcc catgcagag gaagctagt cactttgat caactgcca gctggtgga agacgaggg ccggacgag ggccatgag ggccgccag gtcactggg agagcgcac tgtcactga gcttatttt acaacacca gaaccccca gcagctctc tgccaattt gtgggccgc tgtgcgggc cagaggttg ccagttttt ggggccagc catgcagga agaggtgtt aaaactagt ccttctggc tttggaaga cggctcagc tttatcccg caaagtgtt ggtcatgtt tgtggtgca gcgtttgga gccccattt tcctcaagc ctctaagac cagtatagg ccacgtggt ccagctttt atacagggc gtcttgttt caaagtttc gaagcgtga aggagactc gtctttgat gcaactaaa ggaggaatt tcgcactta tgaagctct tcgtcgcga acacgatgc tcaaattgt tcaaattgc aactgaagc cggtttgcg aatcgctcc agatcaatg gtcggctct tttgtatgg cgatacggc tcataaatc tcacatgca gagcatcat cgacaagtt gcaaactcc tcaatcttt cgctcaatc ggtacagga attagttat tgcgttaca aaggacggg ggatcccgg gaagttgtc agttttgag aactcctct ggaattatt ggcggcgat tgatcctag tccagaatc atcaactcc atcatggaa agaatgtag tgaagccct cgaggccgt tcgcctagt cgtagcagg cctagtaga atttattca tcaacatgg tagtcgaaa attacaaga tacagcaca tccacaaca tgccaaata taaaatcag catgtgccg tgatttaac attgcgggg aaattgtcc tcgaggtac caattgcac gtttgcaca ttccaatga agagttgga gaaatatcg ggcgaaaaa ccgcaagac tataatccg caacaagga acatcgaat cgacagcca atcaccgac cgagaaaat ctaccccca aaccgaaga ctactattc gatgccgaa cacagcgac cgttagccc tgtgcccat cccaaccgt cgtccaaat ccaacggat gggttatga cgttccctt caaccacaa cctgcctcc ctacgcccc acaacccat ctacgccca aacacctcc atatccaca cgacgtcta ccaaactgc cccaccaaa cctagttct agccaacaa cgaccaatt ctacccacc acagactga tttcacaaa cattggcca tcagagcaa ctcgatggt ggagaaaaa atgggataa tgtgatacc gaaaaacca gaatgtgct tcaacacca cacgaaaag atcgtcaac gaagagttt agctgaatt gcatcaacg gaaacagga ggttttgtc gcaactgga gaaagttgt aggaaagac ggcggcaac ggcgatttc acataatgc gacgagtct tgccaggca agagtcgca ccatgatct ggatagtcc aacatcacc gtattcgtt ctggtcagg gcatgtcaa tagtgggcc tacttttgt gcggtcgga ttctattct ggccgctga tgatgacta tgtgccgta tgattcgcc ggcgattag taagtacgg gcccatctc gaggatgca taaggataa tggacaggt ttcaacgtt cagggatgg gaatatgtc atcgtcttt attacgccg ccctcttgc atctgctag tccagtgac ctcattata ctataataa taatccacc gccattcca tcaaggtcc aattccagc tggtgctat tgttggaca tcctggagg tggcatagg atctcctca attgattta tcatccaag agttcaaga cccatccaa ggaactttt ggccgaatc gcaacgagt aaagttgca attgtgcaa tttagaaaa acaattaaa cgacctaaa gttggctac acagggtgt aacaacttt aagcgaacg cgatagact gacacagga actgcaatt aattgaaca agggattca agagaagca gaaagaggc ctgtttgaa taaaatcgt ccattcctt aaaccaagc gatgagcaa ctacaacaa cgccaacgt gaccaattt ggagaattc cgaagaggc gatgtatga agcagatat tgccaacga catgcgcca gttagagat ccgactgca ggaagagat cgaggagca agagaagat atggagcag tgaagagga ctccaacca gtaaattta aacccaatg ggttcagtt cttctttta tttcatgac atgttttcg tgtgggcat gcaagtctg atactattt tgaatcata atgggaata cggttgttg cattttttc ctttatttg ataagacgt atgattcaa aatattgcg atatattca gactgtttt attcacatt aaatacaat aggtgcatt tcgcgtgtt tactgaatt tgatgagta gttgttcca gttttattg gaaagaaac gcggcggcg gtggcggcg tcttttatc actcgctcc agagaacga ttagctgaa atgttatct atctttaat catactgtg tgtgatcgg tatatcaat gttatgagt tgatatatt ttaatttag tgtgcattt gtttgaaaa tgcaactga ctatgaagt tattatgct taaatttaa ttatatagt ctaatttat taagcttta tagagaaat caaatgtgt gttactgtg ttacaaaga aattaccat cactgatgt ttgcgagac attttcaac taagcattt atatttaat atctttgta atataggta atccaacat acatgtata atgtaattt gatgtatgc attcagtga ttatttaga gctgtcatt ttttattgt agtaacgat tctttgtta tatattttt cctacaaaa gtatttatg actatgaaa atttacgcg cgctttatt ctgttttac atttttcct attattata tttatacaa ttgtgtcat atcgtgtgt ttgcaattt atacaaatt gtgtttttc gtttgactt ttacttaat ggtaccaca tttaaaatg tattatcgt tttgttagt aactgagga aatactttt tttaaatgc caaagtatg tttttccaa gtcgtaatg ttctggatg gctcttgtt gtaattgtt tgccacttt tctatcatg ccataaata aataactgc tttgacagc tctgcctta ccaatttat tgtaataag atttaaaat tctttgcta aagcaagtc tgcaatatt agtctaggc tgtgtaacg taattgttt actcttttc gtggttgta cctaaagtt gtttatgtt tgccctata gtcaagttt tatttattg agtcatctg gcattcatt ttattatta ggtcatgtt gcacatgtg attaatatt taactattt atactgatt acattaatc acgtatgta tatttgggt ggaagtata taaattata ccaatattt aaataaatt attattacc cat
