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Gene information
General information
TC006445 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 2270395..2272119
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006445
    iBeetle screen iB_01048
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 3 number 2nd lateral oviduct: 2 - Number of animals showing the phenotype: 2
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006445 RA
      MFWALILAA VLLVTYWLL SRPYKYWTE RGVKQGKPV 
      FIFGDYWGP MLRKQTNAE VVDMLYNQI DKEARYCGI 
      YQFLTPVLL IRDPELIKK ITVKDFEHF VDHRIVIPE 
      ESDPLSGQK WRDMRSTLS PVFTSSKLK YMFSLISER 
      GEQFAQSLL KENKDVITL DIKNSFTRY TNDVMASTM 
      FGITCDSLQ EPKNEFYSL AQKSQDFSG FWKNVKLLG 
      YFLFPEFCK SLEISFYSK EVNSFFEKL VTENIGSRQ 
      KHGIVRPDL IHLLMEAKN HAKKSTGYQ ESNKMKTEI 
      TDQDIATQA LIFFFGGFD TVASLMSFM SYELATNPD 
      CQEKLRQEV DEVMQNCNG KVTYEAIVN MKYMDMVTS 
      ETLRKWPNA PAVDRVCTK PYTIEPARP DEKPIHLKE 
      NDTVLLPIY ALHRDPKYF PNPDRFEPE RFSEENKAN 
      IEPYTYMPF GSGPRNCIG QRFALIETK LFFFYILAN 
      FELIPVERT QIPLKLTKN PFTMTAEKG FWLGFKKR
    CDS sequence
    • >TC006445 RA
      atgttttgg gcacttatc ctggccgct gtcttgctg 
      gtaacctac tggttactc agcaggccc tacaaatac 
      tggaccgaa agaggagtc aaacaggga aaaccagtt 
      tttattttt ggagactac tggggcccc atgctccgg 
      aaacaaacc aacgccgaa gtggttgat atgttgtat 
      aaccaaatt gacaaagag gcaagatat tgcggcatt 
      taccaattt ttaacacct gtgctgctg atcagagac 
      cccgaactt atcaaaaaa atcacagtc aaagatttt 
      gagcacttt gtggaccac agaattgtg atccctgaa 
      gaatccgac cctctatca gggcagaaa tggcgcgac 
      atgaggtcc accctcagc cctgtcttc accagcagc 
      aagctcaag tacatgttt tcgctgatt tccgaaaga 
      ggagaacag ttcgcccaa agtcttttg aaagaaaat 
      aaggacgtt atcaccctt gatatcaaa aattcattc 
      acgcggtac actaacgat gtgatggct agcaccatg 
      ttcggaatc acctgcgac tctctccaa gaacccaag 
      aacgaattc tactctttg gcccagaaa tcgcaagat 
      ttcagcggc ttttggaaa aacgttaaa ttgttgggg 
      tacttcctg tttccagaa ttttgcaag tctctggaa 
      atcagtttc tactccaag gaagtcaac agtttcttc 
      gagaaactc gtcactgag aacatcgga agtcgccag 
      aagcatggc atcgtccgt cccgacctg atccacttg 
      ctgatggag gccaaaaac cacgccaaa aaatccacc 
      ggctaccaa gaatccaac aaaatgaag accgaaatc 
      accgaccaa gacatcgcc acgcaagcc ctcatcttc 
      ttcttcgga ggcttcgac accgtcgcc tccctcatg 
      tccttcatg tcctacgag ctcgccacg aaccccgac 
      tgccaggag aagctccgc caggaagtg gacgaagtc 
      atgcaaaac tgcaacgga aaggtcacc tacgaggcc 
      atcgtcaac atgaagtac atggacatg gtcacttcc 
      gagaccctc cgcaagtgg cccaacgcc ccagccgtg 
      gaccgcgtc tgcaccaag ccctacacc atcgagccg 
      gcgaggccc gacgagaag cccatccat ttgaaggag 
      aacgacacc gtgcttttg cccatttat gcgctacac 
      agggacccc aaatacttc cccaatccg gaccgcttc 
      gagcctgag cgcttcagc gaagaaaac aaagccaac 
      atcgagccc tacacctac atgccgttc gggtccggg 
      cccaggaac tgcatcggc cagagattc gcgctcatt 
      gaaaccaaa ctcttcttc ttctacatt ttggccaac 
      ttcgagctg atcccggtt gagaggacg caaatcccg 
      ctcaaactc accaaaaat cccttcacc atgaccgca 
      gaaaaggga ttctggttg ggattcaag aagcgttaa
    mRNA sequence
    • >TC006445 RA
      attcactcg taaccagtt cggcaatta gtattaatt 
      agtgataag ttttgtgtg atttgtaca atgttttgg 
      gcacttatc ctggccgct gtcttgctg gtaacctac 
      tggttactc agcaggccc tacaaatac tggaccgaa 
      agaggagtc aaacaggga aaaccagtt tttattttt 
      ggagactac tggggcccc atgctccgg aaacaaacc 
      aacgccgaa gtggttgat atgttgtat aaccaaatt 
      gacaaagag gcaagatat tgcggcatt taccaattt 
      ttaacacct gtgctgctg atcagagac cccgaactt 
      atcaaaaaa atcacagtc aaagatttt gagcacttt 
      gtggaccac agaattgtg atccctgaa gaatccgac 
      cctctatca gggcagaaa tggcgcgac atgaggtcc 
      accctcagc cctgtcttc accagcagc aagctcaag 
      tacatgttt tcgctgatt tccgaaaga ggagaacag 
      ttcgcccaa agtcttttg aaagaaaat aaggacgtt 
      atcaccctt gatatcaaa aattcattc acgcggtac 
      actaacgat gtgatggct agcaccatg ttcggaatc 
      acctgcgac tctctccaa gaacccaag aacgaattc 
      tactctttg gcccagaaa tcgcaagat ttcagcggc 
      ttttggaaa aacgttaaa ttgttgggg tacttcctg 
      tttccagaa ttttgcaag tctctggaa atcagtttc 
      tactccaag gaagtcaac agtttcttc gagaaactc 
      gtcactgag aacatcgga agtcgccag aagcatggc 
      atcgtccgt cccgacctg atccacttg ctgatggag 
      gccaaaaac cacgccaaa aaatccacc ggctaccaa 
      gaatccaac aaaatgaag accgaaatc accgaccaa 
      gacatcgcc acgcaagcc ctcatcttc ttcttcgga 
      ggcttcgac accgtcgcc tccctcatg tccttcatg 
      tcctacgag ctcgccacg aaccccgac tgccaggag 
      aagctccgc caggaagtg gacgaagtc atgcaaaac 
      tgcaacgga aaggtcacc tacgaggcc atcgtcaac 
      atgaagtac atggacatg gtcacttcc gagaccctc 
      cgcaagtgg cccaacgcc ccagccgtg gaccgcgtc 
      tgcaccaag ccctacacc atcgagccg gcgaggccc 
      gacgagaag cccatccat ttgaaggag aacgacacc 
      gtgcttttg cccatttat gcgctacac agggacccc 
      aaatacttc cccaatccg gaccgcttc gagcctgag 
      cgcttcagc gaagaaaac aaagccaac atcgagccc 
      tacacctac atgccgttc gggtccggg cccaggaac 
      tgcatcggc cagagattc gcgctcatt gaaaccaaa 
      ctcttcttc ttctacatt ttggccaac ttcgagctg 
      atcccggtt gagaggacg caaatcccg ctcaaactc 
      accaaaaat cccttcacc atgaccgca gaaaaggga 
      ttctggttg ggattcaag aagcgttaa tgatataca 
      atgtacgaa atgttttat aataaaatt attttgtgc 
      cc