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Gene information
General information
TC006441 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 2260475..2262216
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006441
    iBeetle screen iB_01047
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006441 RA
      MAPNSRYCG MYQFTMPTL VVRDPDLIK QITVKDFDY 
      FLNHRTFIP EECDALWGK NLFALTNQK WRDMRSTLS 
      PAFTGSKMK FMFPLISET AEYFIRHFL SKQEDVVTV 
      EMKDAFTRF TNDVIANTA FGVSCDSLK ERENEFYLM 
      GKEATDLKG FWKNIKLLL FFLIPQVFK LCKVRFFSK 
      SVANFFTNL IKGNITKRE EFGIVRPDM IYLLMEARK 
      NVSNEPISD EDITAQALI FFFAGFDTV SSAMCFMSY 
      ELATNPEIQ EKLLQEIDS VEGKPTYDT LMNLKYLDM 
      VVSETLRKW PISVATDRI CNKPYTIEP KSPDKRPLL 
      LEKNLAVWI PVYALHRDP NYFPDPNRF DPERFNEEN 
      KANIKPYTY LPFGLGPRN CIGSRFALL EMKILFFYI 
      LSHFEITPI PRTQIPLKI NKTQFALTA EDGFWLGLK 
      RRQK
    CDS sequence
    • >TC006441 RA
      atggcacct aattccaga tactgtgga atgtatcaa 
      ttcacaatg ccgactctg gtcgtccga gacccagat 
      ttgatcaaa caaatcaca gtaaaagac ttcgattat 
      ttcttgaac catcgcact tttattccg gaagagtgt 
      gacgcactc tggggcaaa aacctcttc gctttgacc 
      aatcagaag tggagagac atgaggagc acccttagt 
      ccggccttc accggcagt aaaatgaaa ttcatgttt 
      ccactaatc tccgaaact gccgaatac ttcataaga 
      cactttttg tcaaagcag gaggatgtg gtaacagtg 
      gaaatgaaa gacgctttc acgcgattt accaacgat 
      gttatcgca aataccgcg tttggcgtc tcgtgtgat 
      tcgctaaaa gagcgcgaa aatgagttt tatttgatg 
      gggaaggaa gccaccgat ctcaaaggt ttctggaaa 
      aatattaaa ttgcttttg ttcttttta atcccccaa 
      gtatttaaa ttatgcaaa gtcaggttt ttctccaaa 
      tcggtggcc aattttttc actaattta atcaagggg 
      aacatcacc aaaagagaa gagtttgga atcgtcaga 
      cccgacatg atttacctc ctaatggaa gctagaaaa 
      aacgtttcg aatgaacca atctccgat gaagacata 
      acggcccaa gccctgatt ttctttttc gccggtttt 
      gacaccgtt tcttcagcc atgtgtttc atgtcgtac 
      gagctagca accaatcca gaaatccag gaaaaacta 
      ctccaggaa atagattca gttgaagga aaaccaaca 
      tacgatact ttaatgaat ttaaaatac ttggatatg 
      gtagtttcc gaaacgcta agaaaatgg ccaataagt 
      gtggccacg gaccggatc tgtaacaaa ccatacaca 
      attgagccc aaatcgcca gacaagaga ccccttctt 
      ttagaaaaa aatctagcc gtttggatc ccagtttac 
      gccctccat agagacccc aactatttt ccggacccc 
      aatcgcttt gatcccgag agatttaac gaggaaaat 
      aaagctaac attaagccc tacacttat ttacctttc 
      ggacttgga ccgaggaac tgtattggg tccagattt 
      gctctcctt gaaatgaaa attttgttt ttctacatt 
      ttgtcccac ttcgagatt actccaata ccacggaca 
      caaatacca ttaaaaatt aataaaaca cagtttgcg 
      ctaacggcc gaagatggg ttttggctg ggtcttaag 
      aggcgtcag aaataa
    mRNA sequence
    • >TC006441 RA
      tgataaatc aaataatat tgaaaataa aactgtgtg 
      cagtggcaa acaccgagc tacttggtc ttgtacaga 
      ataagtaat cgggaaaat ttaataaaa gatgatttt 
      gctgtgttt aattgccgt aatagtgat attaagcta 
      tcggtttct ttaccgacc gcataaata ctggcttga 
      aaggggtgt aattcaagg aaatccaaa acccttact 
      tggacattt catgggcac aacatttca taaacaatc 
      tttcgccga ttgtattca aatggtgta caacatggc 
      acctaattc cagatactg tggaatgta tcaattcac 
      aatgccgac tctggtcgt ccgagaccc agatttgat 
      caaacaaat cacagtaaa agacttcga ttatttctt 
      gaaccatcg cacttttat tccggaaga gtgtgacgc 
      actctgggg caaaaacct cttcgcttt gaccaatca 
      gaagtggag agacatgag gagcaccct tagtccggc 
      cttcaccgg cagtaaaat gaaattcat gtttccact 
      aatctccga aactgccga atacttcat aagacactt 
      tttgtcaaa gcaggagga tgtggtaac agtggaaat 
      gaaagacgc tttcacgcg atttaccaa cgatgttat 
      cgcaaatac cgcgtttgg cgtctcgtg tgattcgct 
      aaaagagcg cgaaaatga gttttattt gatggggaa 
      ggaagccac cgatctcaa aggtttctg gaaaaatat 
      taaattgct tttgttctt tttaatccc ccaagtatt 
      taaattatg caaagtcag gtttttctc caaatcggt 
      ggccaattt tttcactaa tttaatcaa ggggaacat 
      caccaaaag agaagagtt tggaatcgt cagacccga 
      catgattta cctcctaat ggaagctag aaaaaacgt 
      ttcgaatga accaatctc cgatgaaga cataacggc 
      ccaagccct gattttctt tttcgccgg ttttgacac 
      cgtttcttc agccatgtg tttcatgtc gtacgagct 
      agcaaccaa tccagaaat ccaggaaaa actactcca 
      ggaaataga ttcagttga aggaaaacc aacatacga 
      tactttaat gaatttaaa atacttgga tatggtagt 
      ttccgaaac gctaagaaa atggccaat aagtgtggc 
      cacggaccg gatctgtaa caaaccata cacaattga 
      gcccaaatc gccagacaa gagacccct tcttttaga 
      aaaaaatct agccgtttg gatcccagt ttacgccct 
      ccatagaga ccccaacta ttttccgga ccccaatcg 
      ctttgatcc cgagagatt taacgagga aaataaagc 
      taacattaa gccctacac ttatttacc tttcggact 
      tggaccgag gaactgtat tgggtccag atttgctct 
      ccttgaaat gaaaatttt gtttttcta cattttgtc 
      ccacttcga gattactcc aataccacg gacacaaat 
      accattaaa aattaataa aacacagtt tgcgctaac 
      ggccgaaga tgggttttg gctgggtct taagaggcg 
      tcagaaata aaatactgt tgtgactct gtaactggc 
      ttaaataaa cttgtttat tctat