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Gene information
General information
TC006388 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8327544..8350418
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006388
    iBeetle screen iB_01042

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006388 RA
      MPGWYSTGT LYKGLFFLV VILLALEYL FGSITETKH 
      FFVFNATRI PHQEKVVQH SPLVSTLHL KKATKSPPL 
      FNITELPST NNSVESVES ETINATALP PCPDVTNSL 
      RGRIEVLKA PVPSVQELE GRFSWLRPG GHWAPDTCK 
      VPKSVALII PFRCRGEHL LLFLQHMHP FLKRQQLDY 
      TIYIVEQDG DGPFNRAML MNIGFKEAL KMRNYDCFI 
      FHDIDLLPE DDRNLYTCP PGQPRHMSV AVDIFKYRL 
      PYPAIFGGV SAINREHFE LLNGFSNSF WGWGGEDDD 
      MSNRIRYHN LYISRYPLT IARYTMLTH KKDKPSPNR 
      YDMLKQGPK RFDKDGLNS LDYKLIQSK KNLLYTWVL 
      VGIEPPR
    CDS sequence
    • >TC006388 RA
      atgcccggc tggtacagc accggcacc ctctacaag 
      gggttgttc ttcttggta gtcatacta ctggcgctc 
      gaatacctc ttcggatcg atcaccgag acgaaacat 
      ttcttcgtt tttaacgcc acgaggatt ccgcaccag 
      gaaaaggtg gtccagcac agccccctt gtctccacg 
      ctccatctc aagaaagcc accaaaagt cccccactg 
      ttcaacatc accgaactt ccctcaacg aacaattcc 
      gtcgaatcg gtcgaaagc gaaacgatc aacgccacg 
      gccttgccc ccctgtccc gacgtcacc aacagcctc 
      cgcgggagg atcgaggtg ctgaaggcg cccgtcccc 
      agcgtccag gagctggag gggcggttc tcctggctg 
      cgcccgggg gggcactgg gcgcccgac acgtgcaaa 
      gtccccaag agtgtggcg ttgattatt ccgtttcgc 
      tgccgtggg gagcatctc ctgctgttt ctccagcac 
      atgcatccg ttcctgaag aggcagcag ctcgactat 
      acgatctac atagtcgag caagatggc gacggaccc 
      tttaaccgc gccatgttg atgaacatt gggttcaaa 
      gaagcgctc aaaatgcga aattatgat tgttttatc 
      tttcacgat attgatttg ttgccggaa gacgaccgg 
      aatttgtac acgtgtccg ccgggacag cccaggcac 
      atgagcgtc gcggtggac attttcaag tataggctg 
      ccttatccg gccattttc ggcggggtg tcggcgata 
      aatagggaa cacttcgag ttgttgaac ggcttttcc 
      aactcgttt tggggttgg ggaggcgaa gacgacgac 
      atgtcgaat aggattagg tatcataac ttgtacatc 
      tcgcgatat ccgttgaca atagcaaga tacacgatg 
      ctgacgcac aaaaaggac aagccgagc cctaacaga 
      tacgacatg ctgaagcag ggacccaaa cgcttcgac 
      aaggacggg ctcaacagc ttggattac aaactgatc 
      caaagcaaa aagaatctc ttatacacc tgggttttg 
      gtggggatc gagccgccg aggtga
    mRNA sequence
    • >TC006388 RA
      tgcgcgtcg cgacgccat cctggagga gatccctcg 
      ccgggatga atgcggatc tgcgtctag acaaggagg 
      gcgaatcga gcgtcgata tgaggccag ctgttacgc 
      accaggtcc ggactcggc aagcaaacg agcgtcgtt 
      tgacgtgcg gtaacgcga tataaccac cgctaatga 
      aaagataat aatttgaaa accgcaaca atcgattac 
      gaaacgtca cgcgatcga gagaaagta aacgaaaga 
      gaaatttac ttaaactta acatatacg cacacttcg 
      atctgttcc ggtcctgtg ccctccacc ctccagccg 
      atcctcccg aaatttcca agttaaaaa caatactct 
      tgaaaaaat cgtaacgta tgtcctggc tagagttgt 
      ccggttgaa gtggagggg agactacct accggattt 
      taacatttc gcatttctt ctccgggtt gcggtgatg 
      tcagtgtgg tgcgagtgt atcgtgtga aatgtacca 
      acgataagt gagctggag gatgctttg tcccataca 
      aactaggta agggtgggt gatgcccgg ctggtacag 
      caccggcac cctctacaa ggggttgtt cttcttggt 
      agtcatact actggcgct cgaatacct cttcggatc 
      gatcaccga gacgaaaca tttcttcgt ttttaacgc 
      cacgaggat tccgcacca ggaaaaggt ggtccagca 
      cagccccct tgtctccac gctccatct caagaaagc 
      caccaaaag tcccccact gttcaacat caccgaact 
      tccctcaac gaacaattc cgtcgaatc ggtcgaaag 
      cgaaacgat caacgccac ggccttgcc cccctgtcc 
      cgacgtcac caacagcct ccgcgggag gatcgaggt 
      gctgaaggc gcccgtccc cagcgtcca ggagctgga 
      ggggcggtt ctcctggct gcgcccggg ggggcactg 
      ggcgcccga cacgtgcaa agtccccaa gagtgtggc 
      gttgattat tccgtttcg ctgccgtgg ggagcatct 
      cctgctgtt tctccagca catgcatcc gttcctgaa 
      gaggcagca gctcgacta tacgatcta catagtcga 
      gcaagatgg cgacggacc ctttaaccg cgccatgtt 
      gatgaacat tgggttcaa agaagcgct caaaatgcg 
      aaattatga ttgttttat ctttcacga tattgattt 
      gttgccgga agacgaccg gaatttgta cacgtgtcc 
      gccgggaca gcccaggca catgagcgt cgcggtgga 
      cattttcaa gtataggct gccttatcc ggccatttt 
      cggcggggt gtcggcgat aaataggga acacttcga 
      gttgttgaa cggcttttc caactcgtt ttggggttg 
      gggaggcga agacgacga catgtcgaa taggattag 
      gtatcataa cttgtacat ctcgcgata tccgttgac 
      aatagcaag atacacgat gctgacgca caaaaagga 
      caagccgag ccctaacag atacgacat gctgaagca 
      gggacccaa acgcttcga caaggacgg gctcaacag 
      cttggatta caaactgat ccaaagcaa aaagaatct 
      cttatacac ctgggtttt ggtggggat cgagccgcc 
      gaggtgatg aagctacat ttcttcgtt tttgcgtta 
      cttcggttg atttgtact tatatgagt tcctttaaa 
      tgtaacgtt ttttttatt tattgagac agacatcga 
      gggacttgt ctggatcca aggtgataa tagatttta 
      aaatgaaac attccaccc aaacgattc gaatttttt 
      ctagtcatt acattcctt actagaatt ccattagga 
      aagcgcgac ttaagtagt cttccatgt tctgttcag 
      gcggttggg tggcttttg tagcgtttc aaggtcgag 
      taggcggag cgccgcttc ccccaccac gccagtggc 
      gcccttact actcagacc acagcttgc ttgatttat 
      aaccaaatc taggaagct gtgcctcaa acataataa 
      tctaacgag tgagatttt agagataga gtaggtgcc 
      aaaacgtac caagatcac tcattactt aatttaggc 
      ctagtttcg ttgcgattt tgtggctgt gattccctt 
      ttcttgatc actcatatc gtagtaata cgtaactac 
      ctgcttatt ttcggggcg agctcgaat tgaccgccc 
      cgatttgcg ttcagttaa gtgttccgt caattgaga 
      agatgatat tttgactgt atgtatata tttttaagt 
      aaaacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY