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Gene information
General information
TC006370 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7970271..7979285
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006370
    iBeetle screen iB_01040

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006370 RA
      MDSINKRNG NNDEPKGAQ APKRAPLRQ FAPLMVAMV 
      GLPGRGKSL MAKRLSRYL NYTGDVCKV YDISDYRRK 
      HIKTYASHE IFRADNLPA WRIRQQSFR EALEEAASW 
      LQEPNHHVA ILDGPNVSR AQREEIYDL VYCQLGFRV 
      MFIECVCED PVLLERNFK EILQYSADY CDMATEEAL 
      KDLTQKMAH YKAQYESPS LGSHWELPC PMIKVLDSG 
      EGGVVAHGV VGTKESKIL GYISTPKPI QQTLYFSRH 
      GESEFNVLG KIGGDAPLS PRGRMYAQS LAKHIQALN 
      LPSLQVWTS TLQRTKATS AHINAPKQH LPELDEIYS 
      GDCENLTYE ELQERFPRE LARRDHEKL KYRYPQGES 
      YLDVMRRVV PVLNQLECE SNVLTISHQ AVLRCILAY 
      FLDTPIEEV PYIHVPLHT IIKITLQGY NYNMETVKM 
      PIDCVDTNR AKPLNCSEG RTREEALKT LPAHYDSVA 
      SLNLTYCT
    CDS sequence
    • >TC006370 RA
      atggattca atcaacaag aggaacggc aacaatgac 
      gaaccgaaa ggcgcccaa gcacccaaa agggctcct 
      ttgaggcag ttcgccccc ttgatggtg gccatggtg 
      gggctgcca gggcggggg aagagtctc atggccaag 
      cggctgtcc cgctatctc aactacacc ggggacgta 
      tgcaaagtg tatgacatt agtgactac cgtcgcaaa 
      cacatcaaa acctacgcc tcacatgag atttttcgt 
      gccgataat ctcccagct tggcgtatc cgccaacaa 
      agctttcgg gaagcttta gaagaggca gcttcctgg 
      ttacaagaa cccaaccat catgtggcg attttagac 
      ggccctaat gtctcgcgg gcacaaagg gaggaaatt 
      tatgatttg gtctactgt cagttggga ttccgggtc 
      atgtttatc gagtgtgtt tgcgaagac ccggtctta 
      ctcgaacgc aatttcaaa gaaatcctc caatacagt 
      gccgactac tgcgacatg gccaccgaa gaagccctc 
      aaagaccta acccaaaaa atggcccac tacaaagcg 
      caatacgaa agcccatca ttgggaagc cactgggaa 
      ctaccctgt cccatgatc aaagtcctg gatagtggc 
      gaaggaggc gtagtcgcc catggagta gtcggaaca 
      aaagaaagc aaaattcta ggctacatc tcaaccccc 
      aaacccatc caacaaacg ttatatttc agtcggcac 
      ggcgaaagc gaattcaac gtccttgga aaaatcgga 
      ggtgacgcc cctttatca ccaaggggg cgcatgtac 
      gcccaatcc ttggccaaa cacatccaa gccctaaac 
      ctcccctcg ttgcaagtg tggacgtcg acactccaa 
      aggaccaaa gccaccagt gctcacatc aacgccccc 
      aaacaacac ctgcccgaa ctggacgag atctactcg 
      ggcgactgc gagaatttg acttatgag gagctgcag 
      gagcgtttt ccgcgcgaa ttggcccgc cgagaccac 
      gagaagctc aaataccgt tacccccag ggggagtcc 
      tatttggac gtgatgcga agagttgtc ccagtgctg 
      aatcaactc gaatgcgaa tcgaacgtg ctcacgatt 
      tcacaccag gccgtgctg cgctgcatt ttggcctat 
      ttcctggac acccccatc gaggaggtg ccctacatc 
      cacgtgccc ctccacaca ataatcaag ataactttg 
      caaggctat aactacaac atggagacg gtcaaaatg 
      ccgatcgat tgcgtcgac acaaacagg gccaagccc 
      ttgaactgc tccgaggga cgcacgcgg gaagaggcc 
      cttaagacg ctcccggcc cactacgat tccgttgcc 
      agtctcaat ctcacctac tgtacttag
    mRNA sequence
    • >TC006370 RA
      taatttagc tgaaacata tttttttgt aaccaaaga 
      acatgtttt tttaataat atttggtga aaaacctgt 
      tataataaa ccgaaatga cagagtggt atttagagt 
      ccaaagtcc ggcccgaaa tagcctggc agatgcacc 
      aagtttcga aacgggaca aagtttact gcaatttgg 
      cgaaaaaac tgcaaaaaa ttcaaaaag ccgcgccaa 
      aaatccagt cacgtgacc tgtcaaagt gccaaaaaa 
      gctataatt cgaaatttt ttccacatt tttgtggcg 
      aattgaatg aaaaatgac cgtttcgct caattttaa 
      ccattgtct ggacaaata ttggttcct ggagagtgg 
      gcccaccta tcggcgttt atttatggc tcgacctta 
      tctctctgc gctggtcgt agcctacat cgatcgtat 
      ttatacaaa accgggttg tctctgttt attcgagct 
      gtggttcgt gcgagcagt ggctgcggt tacggtaaa 
      cgaaacaca aaaagattc ctccgcgtg tataaatgt 
      tggaagacg taaagtgcg gtcgaacat cgcattgat 
      ttccgtcaa gagtcttca aaagtccgt aaatctggc 
      gactgaaat tagatacag acttggaat gcgacacat 
      cttgaaaaa gtttatcat acatacgtc ttcagatca 
      tttttcaga gggagcgat cgacattta gttgtttcg 
      ttttttgta ttttttttt cttcaattt tctgttgtt 
      attgagtga ttcgaagtg tgtttgaat tgttttttt 
      gagtcggca aattcattc agaaagtgt tctttttct 
      acaattctg caaccggat atcgaaatg gattcaatc 
      aacaagagg aacggcaac aatgacgaa ccgaaaggc 
      gcccaagca cccaaaagg gctcctttg aggcagttc 
      gcccccttg atggtggcc atggtgggg ctgccaggg 
      cgggggaag agtctcatg gccaagcgg ctgtcccgc 
      tatctcaac tacaccggg gacgtatgc aaagtgtat 
      gacattagt gactaccgt cgcaaacac atcaaaacc 
      tacgcctca catgagatt tttcgtgcc gataatctc 
      ccagcttgg cgtatccgc caacaaagc tttcgggaa 
      gctttagaa gaggcagct tcctggtta caagaaccc 
      aaccatcat gtggcgatt ttagacggc cctaatgtc 
      tcgcgggca caaagggag gaaatttat gatttggtc 
      tactgtcag ttgggattc cgggtcatg tttatcgag 
      tgtgtttgc gaagacccg gtcttactc gaacgcaat 
      ttcaaagaa atcctccaa tacagtgcc gactactgc 
      gacatggcc accgaagaa gccctcaaa gacctaacc 
      caaaaaatg gcccactac aaagcgcaa tacgaaagc 
      ccatcattg ggaagccac tgggaacta ccctgtccc 
      atgatcaaa gtcctggat agtggcgaa ggaggcgta 
      gtcgcccat ggagtagtc ggaacaaaa gaaagcaaa 
      attctaggc tacatctca acccccaaa cccatccaa 
      caaacgtta tatttcagt cggcacggc gaaagcgaa 
      ttcaacgtc cttggaaaa atcggaggt gacgcccct 
      ttatcacca agggggcgc atgtacgcc caatccttg 
      gccaaacac atccaagcc ctaaacctc ccctcgttg 
      caagtgtgg acgtcgaca ctccaaagg accaaagcc 
      accagtgct cacatcaac gcccccaaa caacacctg 
      cccgaactg gacgagatc tactcgggc gactgcgag 
      aatttgact tatgaggag ctgcaggag cgttttccg 
      cgcgaattg gcccgccga gaccacgag aagctcaaa 
      taccgttac ccccagggg gagtcctat ttggacgtg 
      atgcgaaga gttgtccca gtgctgaat caactcgaa 
      tgcgaatcg aacgtgctc acgatttca caccaggcc 
      gtgctgcgc tgcattttg gcctatttc ctggacacc 
      cccatcgag gaggtgccc tacatccac gtgcccctc 
      cacacaata atcaagata actttgcaa ggctataac 
      tacaacatg gagacggtc aaaatgccg atcgattgc 
      gtcgacaca aacagggcc aagcccttg aactgctcc 
      gagggacgc acgcgggaa gaggccctt aagacgctc 
      ccggcccac tacgattcc gttgccagt ctcaatctc 
      acctactgt acttagtta ttcgttcgg gatggctta 
      ttttacatt gttttaaat gttttagct tgaatattt 
      agtgttttg tgtagctga ggaacattt gctggacga 
      tagcaataa ttggatgta tagtattta actg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY