| General information | |||
| TC006361 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 7916810..7920604 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006361 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01039 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (16 dpi)
pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (22 dpi)
- >TC006361 RA
MTVPTPSEK LLTNGSTTP KRQNGQKSG LMKLSSYVL ALRPWSLSA SLMPTLLGS TIAYKYPGV TDFNYATLL LTIFTVVSV HGAGNVVNT YFDYVKGID NRKSDDRIL VDHILTKDE VVSLGALLY FAGCVGFIL LAALSPAKM EHLALVYFG GLSSSFLYT GGIGFKYIA LGDVLILIL FGPISVLFA FMSQTGRVE WATIYYAIP LALNTEAIL HSNNTRDSE SDKKVGIVT LAIIIGHTA SHVLYAFLL FTPYIMFMV ASLKYSKWF MLPLVTLPS AFKIEKQFR SNDIHSVPK KTAKLNLFF GVLYVLACS LVPALPYVT KR
- >TC006361 RA
atgacggtg cccaccccc agcgagaaa ctcctcacc aatggctcc accactccc aagcgccaa aacggccaa aaatcgggc ctcatgaag ctatcaagt tacgtgcta gccttgcgt ccgtggtcc ctcagtgcc agtctcatg cccacactg ttgggctcc accatagcg tacaaatac cccggagtt accgatttc aactacgcc accctcctg ctcacaatt ttcaccgtg gtgtcggtg cacggcgcc ggaaacgtc gtcaacacc tacttcgac tacgtcaag gggatcgac aaccggaaa tcggacgac cggatcctg gtggaccac attctgacc aaagacgag gtggtgtct ctgggggcc cttttatac tttgccggc tgtgtgggg ttcattctc ttggcggcg ttgagcccc gcgaagatg gagcacttg gctttggtc tatttcggg gggctgtcg tcgagcttc ctctacacg ggaggtata ggcttcaag tatatagcc ctcggggat gtgcttatt ttgattttg ttcggcccc atttcggtg cttttcgcc ttcatgtcg caaacgggg cgcgtcgag tgggcgacg atttactac gccatccct ctggccctt aatactgag gcgatttta catagtaat aacacaagg gactctgag tcggacaaa aaggtggga attgtgact ctggcgatt attatcggc cacactgcc tcgcacgtc ttgtacgcg tttttgttg ttcaccccg tatatcatg ttcatggtg gcctcgttg aaatactcg aaatggttc atgttgcct ctagtcacg cttcctagc gcttttaaa atcgaaaaa cagttcaga tccaacgat atacacagt gtgcccaaa aagactgcc aaattgaat ttgtttttc ggtgttttg tacgtgctg gcctgtagt ttggttcca gcgctgccg tacgtcacc aaaagatag
- >TC006361 RA
tgtcaaatg tcataaaaa caccaactt ttgtaagaa aaataattt atttctgat ttaaaaaag tacacaatt ctcgattat caacaatgg ttaatgtaa tgaaaggcg ttttggtga cctgtgacc aggccatga aacaattcc ttttgtatt tagatgaga ctacaaaac taggccgaa aattcataa ttcaggact tggatgaaa accacttat ttatttcag ccgatattt tagagacat tacaggcaa aaatcgacg atctgatgg accaaatta gcttcccag tcgccgaaa aaagcaatt aattttttg taatttgca aaagtgagg ttatgtcat attgatgtg tggtcattc gaattataa ataaaaagc gcagcattt atataatga cgtgttttg ctataaata atatcgttg cgggccggt tttcgtcca tacttacgc aataaggca ctaattaac tgttttttc aaattgtcg gtacaaata atacagttc cctgtaaat tggaagtgg ctacaatta cgcttcttt gataaccga ctattctac agttgttac gccatcacg aacattttt acacacaaa tgataagaa tagaattaa gcaatagat aacggaaac gtgtcacta attataatt tttccctga tttaatagc cgccgtgac gtaaccaca gccacatgt caaacgtca caattgact tctttggtt atgttccgt ttgtcaaag ttataaaac gaaaaaacg cgtttctcg gccacaaat catcaagtg tgttaagtt ccgtgtcag tgcatttac ccccatcat gacggtgcc cacccccag cgagaaact cctcaccaa tggctccac cactcccaa gcgccaaaa cggccaaaa atcgggcct catgaagct atcaagtta cgtgctagc cttgcgtcc gtggtccct cagtgccag tctcatgcc cacactgtt gggctccac catagcgta caaataccc cggagttac cgatttcaa ctacgccac cctcctgct cacaatttt caccgtggt gtcggtgca cggcgccgg aaacgtcgt caacaccta cttcgacta cgtcaaggg gatcgacaa ccggaaatc ggacgaccg gatcctggt ggaccacat tctgaccaa agacgaggt ggtgtctct gggggccct tttatactt tgccggctg tgtggggtt cattctctt ggcggcgtt gagccccgc gaagatgga gcacttggc tttggtcta tttcggggg gctgtcgtc gagcttcct ctacacggg aggtatagg cttcaagta tatagccct cggggatgt gcttatttt gattttgtt cggccccat ttcggtgct tttcgcctt catgtcgca aacggggcg cgtcgagtg ggcgacgat ttactacgc catccctct ggcccttaa tactgaggc gattttaca tagtaataa cacaaggga ctctgagtc ggacaaaaa ggtgggaat tgtgactct ggcgattat tatcggcca cactgcctc gcacgtctt gtacgcgtt tttgttgtt caccccgta tatcatgtt catggtggc ctcgttgaa atactcgaa atggttcat gttgcctct agtcacgct tcctagcgc ttttaaaat cgaaaaaca gttcagatc caacgatat acacagtgt gcccaaaaa gactgccaa attgaattt gtttttcgg tgttttgta cgtgctggc ctgtagttt ggttccagc gctgccgta cgtcaccaa aagatagat gtacgagct cttattagt tgtatttat ttctattaa gttattttt tactgtgtt gaggaactg cgtcaaaag ggggcgcta gtgcgggtt tgcggcttg gggtccaag ccgcaatac cttctcatt ttcgtttgt tacgtcgag attattgta cttatgaca atgaacagt cacgatcat aaagaatgt catccctat aaactgttg ggtcgtaaa cgtcaataa acatgattt cgggttttt acttttgta acatcaacg atattctaa tcgtgtaaa ttaataaat gtagtctat ttactccaa gtatgatca aaactgtat tacgtaatg tgttagctt tttaagtag tacttattt acttatttt taaacaaaa gtaggtgta ctaaagttc aaaaccaac gttagttac attctgttc cagttgtta gccactttt aatttaaaa ttttaaata gtaccctct attttttga aacattttt gttataaat ttttactta tctttaaat aatcttttt tttgttaga aattttgcc tttaattta atgtttcta aaagtccta gtcttttgt caagtattt accaataaa attctaaaa aaaagtgat tatacaaaa ggacgaagt cgctcacag acaaaccga aaatggggt cttacttaa aacgaatca tccgttttt aaccgcaag ttgttaaag aaactcggc ttcgcctcg gctggaatt tttctttat tcaaaacgg ttgcaaaaa ataataacg atgtttctc catttatgg attctatca atatgaact tactaatat ttggtcctt cgagccgga taaagcagc catgttggc cgtgg
