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Gene information
General information
TC006355 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 733767..743027
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006355
    iBeetle screen iB_01038
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006355 RA
      MSANIFPMM SSKPKESRI PIPSPVPLR RSGSVRLRA 
      KSPDSNRTA DGLQRGLSF MERGERYAK HGAPGIIRP 
      KASPESPSR IRSLSLSLS TNRLVPSTP PTTPDGPLC 
      EWDSESLTS VTSVGSCDH ASVARNGTT FSGRSMKYV 
      FHCSQHSGA TGEDYLTPT QRLHRQVKK LKNLLHQAR 
      KELEDKDSD ILKLTKEVV ELRLYKAAL NSPEDRSNS 
      SDAVTVREN TDEQITPDV DTTDRSVVQ NEMTNSYTD 
      SGHFEDYAN SPVATRSPM KRKMDLADK AITAALGDN 
      NEHTNLILE YERRLQELV KTHEEETYQ LKQKHNDKI 
      EELLQRITE INKRYWELV PELDLAKSK IKELESQLE 
      EACQKLAQQ EEKQKKTYL EMYRQGHEA ANLERENRV 
      MEIAKQGPS RISVPELLE ELQVTKNEL ENIKDLEYA 
      STSNNLQPL LSAKEALSL WVLGARKAM YRQLIEAKN 
      KSKIDPEIT LQFLKSAVY YFLTDKENS QGHLKAIQS 
      ILGFTPNEI TSIDKARLS
    CDS sequence
    • >TC006355 RA
      atgagcgca aacattttc ccgatgatg tcgtccaag 
      ccgaaggag tcccggatt cccatcccg tcccccgtc 
      ccgctgcgc cgctccgga agcgtccga ctccgggcg 
      aaaagcccg gactcgaac cgcactgca gacggccta 
      caacgtggg ttgtccttt atggagagg ggtgagcgg 
      tatgccaaa catggcgct cccggaatc atccggccc 
      aaagcgagc ccggagtcg ccgtcccgc atccggagt 
      ctgagtttg tcgctgtcg acgaaccgt ctggtgccc 
      agcacgccg cccacgacc cccgatggg cccctttgc 
      gagtgggac agcgagagc ctcaccagt gtgaccagc 
      gtggggtca tgcgaccac gcgagtgtg gctcggaat 
      ggcaccacg ttctccggg aggagcatg aagtacgtg 
      tttcactgt agtcaacat tcgggggcc actggcgag 
      gactatctg actccgacg cagaggctg caccggcag 
      gtgaagaag ttgaagaat ttgctgcat caggccagg 
      aaggagctt gaggataaa gatagtgat attttgaaa 
      ttgaccaaa gaggtggtc gagttgagg ctgtacaaa 
      gctgcgctc aattcaccg gaagatagg tccaattcg 
      agtgatgcg gtcactgtt agggaaaat accgacgaa 
      cagattacg cccgatgtt gatacgact gataggagt 
      gttgtgcag aatgaaatg accaattcc tatacggat 
      tcggggcat tttgaggat tatgccaat tcaccggtt 
      gccacaagg agtccgatg aagcgcaaa atggacttg 
      gccgacaag gccataaca gcggccctc ggcgacaac 
      aacgaacac accaaccta atcctcgaa tacgagcgc 
      cgactgcaa gaactggtc aagacacac gaggaagaa 
      acctaccaa ctcaaacaa aaacacaac gacaaaatc 
      gaagaactc ttacaaaga atcaccgaa atcaacaaa 
      cgctactgg gaattagtc cccgaatta gacttagcc 
      aaatcaaaa ataaaagaa ttagaatca caactggag 
      gaagcctgc caaaagctg gcccaacag gaggagaaa 
      cagaagaag acttacctc gaaatgtac cggcaaggg 
      cacgaggcc gcgaatcta gaacgcgaa aatagggtg 
      atggaaatc gccaaacaa gggccaagc cgcatttcg 
      gtgcccgag cttttagaa gagttacaa gtgacgaag 
      aacgagctt gaaaacatc aaggactta gagtacgcc 
      tcaacttct aacaactta cagcctttg cttagcgct 
      aaagaggct ctctcgttg tgggtactt ggtgccaga 
      aaggcaatg taccgccag ttgatcgaa gccaaaaac 
      aagagcaaa atcgatccg gaaatcacc ctccaattc 
      ctcaaatcc gccgtttat tactttctc acagacaag 
      gagaacagt cagggtcac ttgaaagcc attcagagc 
      atcctaggc ttcacacct aacgaaatt actagtata 
      gataaggct aggctgtcc taa
    mRNA sequence
    • >TC006355 RA
      ttacgctac aaaagtggg ggcaatact cccagaacg 
      ttccgtaat actctgttg aaataataa ttaactttc 
      gacgttcta aatccaacc acaacaata tgttcccga 
      gcaaacgca agtgatcaa atggtgcta ccgcaacaa 
      tcagaattc gcaccatga gcgcaaaca ttttcccga 
      tgatgtcgt ccaagccga aggagtccc ggattccca 
      tcccgtccc ccgtcccgc tgcgccgct ccggaagcg 
      tccgactcc gggcgaaaa gcccggact cgaaccgca 
      ctgcagacg gcctacaac gtgggttgt cctttatgg 
      agaggggtg agcggtatg ccaaacatg gcgctcccg 
      gaatcatcc ggcccaaag cgagcccgg agtcgccgt 
      cccgcatcc ggagtctga gtttgtcgc tgtcgacga 
      accgtctgg tgcccagca cgccgccca cgacccccg 
      atgggcccc tttgcgagt gggacagcg agagcctca 
      ccagtgtga ccagcgtgg ggtcatgcg accacgcga 
      gtgtggctc ggaatggca ccacgttct ccgggagga 
      gcatgaagt acgtgtttc actgtagtc aacattcgg 
      gggccactg gcgaggact atctgactc cgacgcaga 
      ggctgcacc ggcaggtga agaagttga agaatttgc 
      tgcatcagg ccaggaagg agcttgagg ataaagata 
      gtgatattt tgaaattga ccaaagagg tggtcgagt 
      tgaggctgt acaaagctg cgctcaatt caccggaag 
      ataggtcca attcgagtg atgcggtca ctgttaggg 
      aaaataccg acgaacaga ttacgcccg atgttgata 
      cgactgata ggagtgttg tgcagaatg aaatgacca 
      attcctata cggattcgg ggcattttg aggattatg 
      ccaattcac cggttgcca caaggagtc cgatgaagc 
      gcaaaatgg acttggccg acaaggcca taacagcgg 
      ccctcggcg acaacaacg aacacacca acctaatcc 
      tcgaatacg agcgccgac tgcaagaac tggtcaaga 
      cacacgagg aagaaacct accaactca aacaaaaac 
      acaacgaca aaatcgaag aactcttac aaagaatca 
      ccgaaatca acaaacgct actgggaat tagtccccg 
      aattagact tagccaaat caaaaataa aagaattag 
      aatcacaac tggaggaag cctgccaaa agctggccc 
      aacaggagg agaaacaga agaagactt acctcgaaa 
      tgtaccggc aagggcacg aggccgcga atctagaac 
      gcgaaaata gggtgatgg aaatcgcca aacaagggc 
      caagccgca tttcggtgc ccgagcttt tagaagagt 
      tacaagtga cgaagaacg agcttgaaa acatcaagg 
      acttagagt acgcctcaa cttctaaca acttacagc 
      ctttgctta gcgctaaag aggctctct cgttgtggg 
      tacttggtg ccagaaagg caatgtacc gccagttga 
      tcgaagcca aaaacaaga gcaaaatcg atccggaaa 
      tcaccctcc aattcctca aatccgccg tttattact 
      ttctcacag acaaggaga acagtcagg gtcacttga 
      aagccattc agagcatcc taggcttca cacctaacg 
      aaattacta gtatagata aggctaggc tgtcctaag 
      ttcagactg tgcctaatt attacgcac gttttctct 
      atgttacgc actctagtg gtaaaaacg gtccgacgg 
      aaagtgata aagctattt tttcaaggt tgcctttga 
      gagagatta gttgagtat tgatattta attagcaaa 
      agtttattg ttaccgagc atattctaa agagggact 
      ggtgcaatc tgacgtaat acgcatttt acgcgcttt 
      cttttgtta gaaaatgac tgttatatt tgtaagttt 
      atttattgg tgttcaatc gttacgaat aaagtatat 
      ctaaacgc