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Gene information
General information
TC006352 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 685307..690042
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006352
    iBeetle screen iB_01036
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    tropharium partially degenerated - Number of animals showing the phenotype: 2

    egg chamber number decreased - Number of animals showing the phenotype: 2
    egg chamber sometimes segmentation/division not present - Number of animals showing the phenotype: 2
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006352 RA
      MEYFRSGLK SVLGAPAPG TQPTGAETV ERLVSRVST 
      STLLEDRRD ACRALKALS RKYRLEVGA QGMDVLRQV 
      LELDRSDCE IIGYCLDTL CNITSKEVF EEESESNLN 
      IKVNVGEQF TEMFIKNAE NVTLVLSFL EEYDFRVRW 
      PAVKLLTSL LASKPKEIQ DVILVSPMG VSKLMDLLV 
      ESREVIRND ALLLLINLT KSNANIQKI VAFENAFDR 
      LFDVIREEG FTDGGIVVE DCMLLMLNL LKNNTSNIN 
      FFKEGSYIQ KLAPMFILP DNLEEVGWS PQKVSNMHC 
      VLQLVRTLV SPTNPVQIV TSCQKTMRN ANLLEALCN 
      ILMASGVPA DILTETINT VGEVIRGNL TNQEYFAGV 
      MAPSNPPRP AIVVLLMSM VNEKQPFTL RCAVLYCFQ 
      CFLFKNEIG QEQVVQTLL PSSTNSTLT TGQLLCGGL 
      FSADSLSCW FSSVALSHT LIENPAQRE QLLRVLLAT 
      NLGSQPVSL LHQCAVFLQ QTTKLQAKI GILMLLAMW 
      MSHCPQAVQ VFLNVPGAM AFLTAQTSA SEFDNNEEI 
      LQGLCAFLM GLCVAFNDN SVQNYSKEN LSQLIEKRV 
      GTETYCTKL GEISRHEVY AKAAKQPQI RAKHSSELL 
      LEFEFCKLF KALEPVIIK ALGVQRDLS NGMSELSLS 
      EHENALLLQ YKELIRDQD KKLQDVHKS LSNLQRENQ 
      DLKGQIEEL RQTNSQLHD QNTLLKAQM QLSGTAVAE 
      PGNDKVKQL EAELVEKDA ELERLRKDQ DDLLELLTD 
      QDTKLNAFK NRLKELGET VEDGDSEDN SAGSDNEV
    CDS sequence
    • >TC006352 RA
      atggagtac ttccggagc ggtttgaag tcggtgctg 
      ggggccccg gcccccggc acccagccc acgggtgcc 
      gagaccgtg gagcggctt gtgagtcgg gtcagtact 
      tcgacgtta ctggaggac cgtcgcgac gcatgtcgg 
      gcccttaag gccctgtct cggaaatat cgcctcgaa 
      gtgggggcc caaggcatg gatgtgttg agacaggtc 
      ctggagttg gaccgctct gactgcgaa atcatcggc 
      tattgtctc gatacgttg tgtaatatc acctcgaag 
      gaggttttt gaggaggag agtgagagt aatttgaac 
      attaaggtc aatgtgggg gagcagttc accgaaatg 
      tttatcaaa aatgccgaa aatgtgacc ctggttttg 
      agttttttg gaggagtac gactttagg gttagatgg 
      ccagctgtc aagctgctc acttcactt ctggcaagt 
      aaacctaaa gaaatccaa gacgtgatc ctcgtgagc 
      cccatgggg gtttcaaaa cttatggat ttgttggtc 
      gaaagtcgc gaagtgatc cggaacgac gctttgctt 
      cttttgatt aacttgaca aaatcaaac gccaacatc 
      caaaaaatt gtggctttt gaaaacgcc ttcgaccga 
      ttgtttgat gttatacgg gaagagggt tttacagat 
      gggggcatt gtggttgag gattgcatg ctactgatg 
      ctcaacctc ctcaaaaat aacacaagc aacatcaat 
      tttttcaaa gaaggatca tacatacag aagttagcc 
      cccatgttt atcctcccg gacaacttg gaggaagtt 
      gggtggagc ccccagaaa gtgtcaaac atgcattgc 
      gtcctgcaa ttggtcagg actctagtc tcgcccaca 
      aacccagtc cagattgtc acttcgtgc caaaagaca 
      atgcgaaat gccaattta cttgaagcc ctttgtaat 
      attttaatg gcaagtggg gtccccgcc gatatttta 
      accgagact attaacaca gtgggcgag gtaataagg 
      ggcaatttg accaatcag gagtacttt gcgggtgtc 
      atggcgcct tcaaacccc ccacgaccc gccatagta 
      gtactacta atgtccatg gtcaacgaa aagcaaccc 
      tttacccta cgttgcgct gttttgtat tgcttccaa 
      tgtttcctc ttcaaaaac gagattggc caggaacaa 
      gtggtccaa accctcctc cctagctcc accaactcg 
      actctaacc accgggcaa ctcctctgt gggggtctg 
      ttcagtgca gactccctc agttgctgg ttcagttcg 
      gtggctcta agtcacacc ctgatcgaa aaccccgct 
      cagagagaa caactcctg agagtcctc cttgccaca 
      aaccttggc tcccaaccc gtcagcctc ctgcaccaa 
      tgtgccgtt ttcctccaa caaaccacc aaactccag 
      gccaaaatc ggtattctg atgctgtta gccatgtgg 
      atgagtcac tgtccccaa gccgttcaa gtcttcctg 
      aacgtgcca ggggctatg gccttcctc acagcgcag 
      acaagcgcc agcgaattc gacaataac gaggaaata 
      ttgcaaggt ctatgcgcg tttttgatg ggtctctgc 
      gttgccttt aacgataac tcggtccaa aactactcg 
      aaagaaaac ttgtctcaa ctaattgag aagcgagtg 
      gggactgag acgtattgt acgaaactg ggggagatt 
      tcgagacat gaagtgtac gccaaagcg gcaaaacaa 
      ccccaaatt cgggccaaa cattccagt gagttgcta 
      ctagagttt gagttttgt aagctcttc aaggcgcta 
      gagcctgtc attattaag gctttgggg gtgcaaagg 
      gacttgtcc aatgggatg tccgaactg agtttgagt 
      gaacacgaa aatgcctta ctgttgcaa tacaaagaa 
      ttgataagg gaccaagac aagaagttg caagatgtg 
      cataaaagc ttgagtaac ttgcaacgg gagaatcag 
      gatttgaag ggacagatt gaggagttg cgtcagacc 
      aactcacag ctccatgac caaaacacg ttacttaag 
      gctcagatg cagttgtcg ggaacggca gtggctgag 
      cctggtaat gacaaagtc aagcaactc gaagctgag 
      ttggttgaa aaagacgca gagttggag aggttgagg 
      aaggatcag gatgatttg ttggagttg ttgacggat 
      caagacacc aagctaaat gcatttaaa aataggttg 
      aaagagttg ggtgaaact gttgaagat ggcgattct 
      gaggataac tcggcgggg tctgataat gaggtgtaa
    mRNA sequence
    • >TC006352 RA
      ctgcgctat tgacgtttc tttgtatac atgtcacgt 
      caaaatgtc aaaaatttc aaacaattt gtgttaatt 
      tcgtgaaat ggagtactt ccggagcgg tttgaagtc 
      ggtgctggg ggccccggc ccccggcac ccagcccac 
      gggtgccga gaccgtgga gcggcttgt gagtcgggt 
      cagtacttc gacgttact ggaggaccg tcgcgacgc 
      atgtcgggc ccttaaggc cctgtctcg gaaatatcg 
      cctcgaagt gggggccca aggcatgga tgtgttgag 
      acaggtcct ggagttgga ccgctctga ctgcgaaat 
      catcggcta ttgtctcga tacgttgtg taatatcac 
      ctcgaagga ggtttttga ggaggagag tgagagtaa 
      tttgaacat taaggtcaa tgtggggga gcagttcac 
      cgaaatgtt tatcaaaaa tgccgaaaa tgtgaccct 
      ggttttgag ttttttgga ggagtacga ctttagggt 
      tagatggcc agctgtcaa gctgctcac ttcacttct 
      ggcaagtaa acctaaaga aatccaaga cgtgatcct 
      cgtgagccc catgggggt ttcaaaact tatggattt 
      gttggtcga aagtcgcga agtgatccg gaacgacgc 
      tttgcttct tttgattaa cttgacaaa atcaaacgc 
      caacatcca aaaaattgt ggcttttga aaacgcctt 
      cgaccgatt gtttgatgt tatacggga agagggttt 
      tacagatgg gggcattgt ggttgagga ttgcatgct 
      actgatgct caacctcct caaaaataa cacaagcaa 
      catcaattt tttcaaaga aggatcata catacagaa 
      gttagcccc catgtttat cctcccgga caacttgga 
      ggaagttgg gtggagccc ccagaaagt gtcaaacat 
      gcattgcgt cctgcaatt ggtcaggac tctagtctc 
      gcccacaaa cccagtcca gattgtcac ttcgtgcca 
      aaagacaat gcgaaatgc caatttact tgaagccct 
      ttgtaatat tttaatggc aagtggggt ccccgccga 
      tattttaac cgagactat taacacagt gggcgaggt 
      aataagggg caatttgac caatcagga gtactttgc 
      gggtgtcat ggcgccttc aaacccccc acgacccgc 
      catagtagt actactaat gtccatggt caacgaaaa 
      gcaaccctt taccctacg ttgcgctgt tttgtattg 
      cttccaatg tttcctctt caaaaacga gattggcca 
      ggaacaagt ggtccaaac cctcctccc tagctccac 
      caactcgac tctaaccac cgggcaact cctctgtgg 
      gggtctgtt cagtgcaga ctccctcag ttgctggtt 
      cagttcggt ggctctaag tcacaccct gatcgaaaa 
      ccccgctca gagagaaca actcctgag agtcctcct 
      tgccacaaa ccttggctc ccaacccgt cagcctcct 
      gcaccaatg tgccgtttt cctccaaca aaccaccaa 
      actccaggc caaaatcgg tattctgat gctgttagc 
      catgtggat gagtcactg tccccaagc cgttcaagt 
      cttcctgaa cgtgccagg ggctatggc cttcctcac 
      agcgcagac aagcgccag cgaattcga caataacga 
      ggaaatatt gcaaggtct atgcgcgtt tttgatggg 
      tctctgcgt tgcctttaa cgataactc ggtccaaaa 
      ctactcgaa agaaaactt gtctcaact aattgagaa 
      gcgagtggg gactgagac gtattgtac gaaactggg 
      ggagatttc gagacatga agtgtacgc caaagcggc 
      aaaacaacc ccaaattcg ggccaaaca ttccagtga 
      gttgctact agagtttga gttttgtaa gctcttcaa 
      ggcgctaga gcctgtcat tattaaggc tttgggggt 
      gcaaaggga cttgtccaa tgggatgtc cgaactgag 
      tttgagtga acacgaaaa tgccttact gttgcaata 
      caaagaatt gataaggga ccaagacaa gaagttgca 
      agatgtgca taaaagctt gagtaactt gcaacggga 
      gaatcagga tttgaaggg acagattga ggagttgcg 
      tcagaccaa ctcacagct ccatgacca aaacacgtt 
      acttaaggc tcagatgca gttgtcggg aacggcagt 
      ggctgagcc tggtaatga caaagtcaa gcaactcga 
      agctgagtt ggttgaaaa agacgcaga gttggagag 
      gttgaggaa ggatcagga tgatttgtt ggagttgtt 
      gacggatca agacaccaa gctaaatgc atttaaaaa 
      taggttgaa agagttggg tgaaactgt tgaagatgg 
      cgattctga ggataactc ggcggggtc tgataatga 
      ggtgtaaat agttttagg tgcaattat ttattgatt 
      aataaaaga ctacacttt ta