General information | |||
TC006352 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 7748537..7753272 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC006352 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_01036 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)
pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
egg chamber number decreased - Number of animals showing the phenotype: 2
egg chamber sometimes segmentation/division not present - Number of animals showing the phenotype: 2
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
larva lethality late (L7) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC006352 RA
MEYFRSGLK SVLGAPAPG TQPTGAETV ERLVSRVST STLLEDRRD ACRALKALS RKYRLEVGA QGMDVLRQV LELDRSDCE IIGYCLDTL CNITSKEVF EEESESNLN IKVNVGEQF TEMFIKNAE NVTLVLSFL EEYDFRVRW PAVKLLTSL LASKPKEIQ DVILVSPMG VSKLMDLLV ESREVIRND ALLLLINLT KSNANIQKI VAFENAFDR LFDVIREEG FTDGGIVVE DCMLLMLNL LKNNTSNIN FFKEGSYIQ KLAPMFILP DNLEEVGWS PQKVSNMHC VLQLVRTLV SPTNPVQIV TSCQKTMRN ANLLEALCN ILMASGVPA DILTETINT VGEVIRGNL TNQEYFAGV MAPSNPPRP AIVVLLMSM VNEKQPFTL RCAVLYCFQ CFLFKNEIG QEQVVQTLL PSSTNSTLT TGQLLCGGL FSADSLSCW FSSVALSHT LIENPAQRE QLLRVLLAT NLGSQPVSL LHQCAVFLQ QTTKLQAKI GILMLLAMW MSHCPQAVQ VFLNVPGAM AFLTAQTSA SEFDNNEEI LQGLCAFLM GLCVAFNDN SVQNYSKEN LSQLIEKRV GTETYCTKL GEISRHEVY AKAAKQPQI RAKHSSELL LEFEFCKLF KALEPVIIK ALGVQRDLS NGMSELSLS EHENALLLQ YKELIRDQD KKLQDVHKS LSNLQRENQ DLKGQIEEL RQTNSQLHD QNTLLKAQM QLSGTAVAE PGNDKVKQL EAELVEKDA ELERLRKDQ DDLLELLTD QDTKLNAFK NRLKELGET VEDGDSEDN SAGSDNEV
- >TC006352 RA
atggagtac ttccggagc ggtttgaag tcggtgctg ggggccccg gcccccggc acccagccc acgggtgcc gagaccgtg gagcggctt gtgagtcgg gtcagtact tcgacgtta ctggaggac cgtcgcgac gcatgtcgg gcccttaag gccctgtct cggaaatat cgcctcgaa gtgggggcc caaggcatg gatgtgttg agacaggtc ctggagttg gaccgctct gactgcgaa atcatcggc tattgtctc gatacgttg tgtaatatc acctcgaag gaggttttt gaggaggag agtgagagt aatttgaac attaaggtc aatgtgggg gagcagttc accgaaatg tttatcaaa aatgccgaa aatgtgacc ctggttttg agttttttg gaggagtac gactttagg gttagatgg ccagctgtc aagctgctc acttcactt ctggcaagt aaacctaaa gaaatccaa gacgtgatc ctcgtgagc cccatgggg gtttcaaaa cttatggat ttgttggtc gaaagtcgc gaagtgatc cggaacgac gctttgctt cttttgatt aacttgaca aaatcaaac gccaacatc caaaaaatt gtggctttt gaaaacgcc ttcgaccga ttgtttgat gttatacgg gaagagggt tttacagat gggggcatt gtggttgag gattgcatg ctactgatg ctcaacctc ctcaaaaat aacacaagc aacatcaat tttttcaaa gaaggatca tacatacag aagttagcc cccatgttt atcctcccg gacaacttg gaggaagtt gggtggagc ccccagaaa gtgtcaaac atgcattgc gtcctgcaa ttggtcagg actctagtc tcgcccaca aacccagtc cagattgtc acttcgtgc caaaagaca atgcgaaat gccaattta cttgaagcc ctttgtaat attttaatg gcaagtggg gtccccgcc gatatttta accgagact attaacaca gtgggcgag gtaataagg ggcaatttg accaatcag gagtacttt gcgggtgtc atggcgcct tcaaacccc ccacgaccc gccatagta gtactacta atgtccatg gtcaacgaa aagcaaccc tttacccta cgttgcgct gttttgtat tgcttccaa tgtttcctc ttcaaaaac gagattggc caggaacaa gtggtccaa accctcctc cctagctcc accaactcg actctaacc accgggcaa ctcctctgt gggggtctg ttcagtgca gactccctc agttgctgg ttcagttcg gtggctcta agtcacacc ctgatcgaa aaccccgct cagagagaa caactcctg agagtcctc cttgccaca aaccttggc tcccaaccc gtcagcctc ctgcaccaa tgtgccgtt ttcctccaa caaaccacc aaactccag gccaaaatc ggtattctg atgctgtta gccatgtgg atgagtcac tgtccccaa gccgttcaa gtcttcctg aacgtgcca ggggctatg gccttcctc acagcgcag acaagcgcc agcgaattc gacaataac gaggaaata ttgcaaggt ctatgcgcg tttttgatg ggtctctgc gttgccttt aacgataac tcggtccaa aactactcg aaagaaaac ttgtctcaa ctaattgag aagcgagtg gggactgag acgtattgt acgaaactg ggggagatt tcgagacat gaagtgtac gccaaagcg gcaaaacaa ccccaaatt cgggccaaa cattccagt gagttgcta ctagagttt gagttttgt aagctcttc aaggcgcta gagcctgtc attattaag gctttgggg gtgcaaagg gacttgtcc aatgggatg tccgaactg agtttgagt gaacacgaa aatgcctta ctgttgcaa tacaaagaa ttgataagg gaccaagac aagaagttg caagatgtg cataaaagc ttgagtaac ttgcaacgg gagaatcag gatttgaag ggacagatt gaggagttg cgtcagacc aactcacag ctccatgac caaaacacg ttacttaag gctcagatg cagttgtcg ggaacggca gtggctgag cctggtaat gacaaagtc aagcaactc gaagctgag ttggttgaa aaagacgca gagttggag aggttgagg aaggatcag gatgatttg ttggagttg ttgacggat caagacacc aagctaaat gcatttaaa aataggttg aaagagttg ggtgaaact gttgaagat ggcgattct gaggataac tcggcgggg tctgataat gaggtgtaa
- >TC006352 RA
ctgcgctat tgacgtttc tttgtatac atgtcacgt caaaatgtc aaaaatttc aaacaattt gtgttaatt tcgtgaaat ggagtactt ccggagcgg tttgaagtc ggtgctggg ggccccggc ccccggcac ccagcccac gggtgccga gaccgtgga gcggcttgt gagtcgggt cagtacttc gacgttact ggaggaccg tcgcgacgc atgtcgggc ccttaaggc cctgtctcg gaaatatcg cctcgaagt gggggccca aggcatgga tgtgttgag acaggtcct ggagttgga ccgctctga ctgcgaaat catcggcta ttgtctcga tacgttgtg taatatcac ctcgaagga ggtttttga ggaggagag tgagagtaa tttgaacat taaggtcaa tgtggggga gcagttcac cgaaatgtt tatcaaaaa tgccgaaaa tgtgaccct ggttttgag ttttttgga ggagtacga ctttagggt tagatggcc agctgtcaa gctgctcac ttcacttct ggcaagtaa acctaaaga aatccaaga cgtgatcct cgtgagccc catgggggt ttcaaaact tatggattt gttggtcga aagtcgcga agtgatccg gaacgacgc tttgcttct tttgattaa cttgacaaa atcaaacgc caacatcca aaaaattgt ggcttttga aaacgcctt cgaccgatt gtttgatgt tatacggga agagggttt tacagatgg gggcattgt ggttgagga ttgcatgct actgatgct caacctcct caaaaataa cacaagcaa catcaattt tttcaaaga aggatcata catacagaa gttagcccc catgtttat cctcccgga caacttgga ggaagttgg gtggagccc ccagaaagt gtcaaacat gcattgcgt cctgcaatt ggtcaggac tctagtctc gcccacaaa cccagtcca gattgtcac ttcgtgcca aaagacaat gcgaaatgc caatttact tgaagccct ttgtaatat tttaatggc aagtggggt ccccgccga tattttaac cgagactat taacacagt gggcgaggt aataagggg caatttgac caatcagga gtactttgc gggtgtcat ggcgccttc aaacccccc acgacccgc catagtagt actactaat gtccatggt caacgaaaa gcaaccctt taccctacg ttgcgctgt tttgtattg cttccaatg tttcctctt caaaaacga gattggcca ggaacaagt ggtccaaac cctcctccc tagctccac caactcgac tctaaccac cgggcaact cctctgtgg gggtctgtt cagtgcaga ctccctcag ttgctggtt cagttcggt ggctctaag tcacaccct gatcgaaaa ccccgctca gagagaaca actcctgag agtcctcct tgccacaaa ccttggctc ccaacccgt cagcctcct gcaccaatg tgccgtttt cctccaaca aaccaccaa actccaggc caaaatcgg tattctgat gctgttagc catgtggat gagtcactg tccccaagc cgttcaagt cttcctgaa cgtgccagg ggctatggc cttcctcac agcgcagac aagcgccag cgaattcga caataacga ggaaatatt gcaaggtct atgcgcgtt tttgatggg tctctgcgt tgcctttaa cgataactc ggtccaaaa ctactcgaa agaaaactt gtctcaact aattgagaa gcgagtggg gactgagac gtattgtac gaaactggg ggagatttc gagacatga agtgtacgc caaagcggc aaaacaacc ccaaattcg ggccaaaca ttccagtga gttgctact agagtttga gttttgtaa gctcttcaa ggcgctaga gcctgtcat tattaaggc tttgggggt gcaaaggga cttgtccaa tgggatgtc cgaactgag tttgagtga acacgaaaa tgccttact gttgcaata caaagaatt gataaggga ccaagacaa gaagttgca agatgtgca taaaagctt gagtaactt gcaacggga gaatcagga tttgaaggg acagattga ggagttgcg tcagaccaa ctcacagct ccatgacca aaacacgtt acttaaggc tcagatgca gttgtcggg aacggcagt ggctgagcc tggtaatga caaagtcaa gcaactcga agctgagtt ggttgaaaa agacgcaga gttggagag gttgaggaa ggatcagga tgatttgtt ggagttgtt gacggatca agacaccaa gctaaatgc atttaaaaa taggttgaa agagttggg tgaaactgt tgaagatgg cgattctga ggataactc ggcggggtc tgataatga ggtgtaaat agttttagg tgcaattat ttattgatt aataaaaga ctacacttt ta
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
