Status
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Gene information
General information
TC006337 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7377109..7381910
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006337
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01035

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006337 RA
      MTTDTVIPL DEVSESSTL TGKHPPKPK IPDGGWGWM 
      VVLSSLIMS LIQDGISFS FGILYTEFV EEFEASKST 
      TSWIGSLFL SVPLLTGPI MSALVDKYG CRAMTILGG 
      LVSATGFIL SYFATNVIT MYVTFGVIS GMGLGLTYI 
      TAVVSIAFW FDKKRNLAV GLGACGTGI GTFVYAPFT 
      TMLIFEYGW RWTVILLAG TLLNLCVCG ALMRDPDWI 
      IEQNKENSK LSSKSSSLS NISHESKKI DLEEIRELL 
      KNGKDAEYI LQTLATSID REDKGQKVE HHQSVLNLP 
      TFIRQNEKV PAEVLEQLQ ENKKLYRII VQNYPSLLH 
      CRSTSEKGL NKMEEAITR IPVTFSLKV KKSDRKAVT 
      HQFSLPENP LAVEKKVKK AETPWLVKQ FSTTTTHPN 
      YFKNMRFPR HSLIHRGAI LNKNKYKLR ASSCPNIYR 
      VSMTTLVKD DDEPWYSGL VKFVNLFDE FHYLLMSVS 
      TVILFAWFM TPYFYLVDH MEASGYTKD EASYVLSGI 
      GVANTIGMV VLGWAGDQS WTNVSKTYG VCLILCGAC 
      CSALYFYTA NYIMVHIFG ALYGIFLAS SFSFTPILL 
      VELLPLETF TKAYGLQLM CQGIGHLGG PPFAALLAD 
      ITNSWSYAF HLSGVMIVL SGILILVIP YTKNRKIFG 
      SGLVEKELA NERA
    CDS sequence
    • >TC006337 RA
      atgaccacc gacacggtg attcccctg gacgaggtc 
      agcgagagc tcgaccttg accggaaaa caccccccg 
      aagccgaaa atccccgac gggggatgg ggctggatg 
      gtggtcctc tcctccctc atcatgtcc ctgatccag 
      gacgggatc agcttctcg ttcgggatc ctctacacg 
      gagttcgtg gaggagttt gaagcctcc aagagcaca 
      acttcctgg atcggaagc cttttcctc tcagtgcct 
      ctgctgacc ggcccgatc atgagtgcc ttggttgac 
      aagtatggc tgccgggcg atgaccatc ctaggcggc 
      ctggtctcg gccactgga ttcatcctg agttatttc 
      gccacgaac gtcatcacc atgtatgtg acgtttggt 
      gtcatttcg ggaatgggg ctaggtctg acgtatata 
      acagcagtg gtgtcaatc gccttctgg tttgataaa 
      aaaaggaac ctagcggtg ggcttaggg gcgtgtggt 
      accgggatt ggtactttt gtctacgcc ccttttacc 
      accatgctg atttttgag tatggttgg cggtggact 
      gtcatcctg ctcgccggg actttactc aacttgtgc 
      gtatgtggc gccctcatg cgggatccc gattggata 
      atcgagcaa aacaaggaa aactccaaa ctgtcgagc 
      aagtcgagc tccctgtcc aatatttcg cacgagtcg 
      aaaaaaatc gaccttgaa gagatacga gagttgttg 
      aaaaacggc aaagacgcc gaatatatc ttgcaaact 
      ttggcgact tcgattgat cgcgaggac aaaggacag 
      aaagttgaa catcaccag tcggtactc aacttgccg 
      acgttcatc cgtcagaac gaaaaagtg ccagcagag 
      gtcctcgaa cagttgcaa gaaaataaa aaactctac 
      cgaattatt gtacaaaat tatcccagt ttgctccac 
      tgccgcagc acttccgaa aaaggcctc aataaaatg 
      gaggaggcc ataacccgc atcccagtg accttctcc 
      ctgaaggtg aaaaaaagc gaccggaag gctgtaacg 
      caccagttc tccctcccc gagaatcct ctggccgtt 
      gagaagaaa gtgaaaaaa gcggagacg ccatggctg 
      gtaaaacaa ttcagtact accaccaca cacccaaat 
      tattttaaa aatatgagg ttccctagg cactcgctt 
      atacaccgc ggcgccatt ttgaataaa aataagtac 
      aagttgagg gcgtcgagt tgtcctaac atataccga 
      gtgtccatg acgacgcta gtgaaagac gacgatgag 
      ccatggtat tcgggtctt gtcaaattc gttaacctt 
      ttcgacgag tttcactat cttctaatg tctgtctct 
      actgtcatt cttttcgct tggtttatg acgccttat 
      ttctactta gtcgaccat atggaagcg tcaggatac 
      accaaagac gaggcttcc tacgtcctg tcagggata 
      ggcgtggcc aacacaatc ggaatggta gtgctcggt 
      tgggccggc gaccaatcg tggaccaac gtctccaaa 
      acgtacggt gtatgcctc atcctatgc ggggcctgt 
      tgctccgcc ctctatttc tacacagcc aattacatc 
      atggtgcac atttttggc gccctctat ggcattttt 
      ctagctagt agtttctcg tttacgccg attctgttg 
      gtggaactg ttgccacta gaaacgttt accaaagcc 
      tacggttta caattgatg tgtcaaggg attgggcat 
      ttggggggt ccaccgttt gccgcacta cttgccgat 
      atcaccaat agttggagt tacgccttt catttgtct 
      ggggttatg atcgtcctt tccgggatc ttgattctg 
      gtgattcct tatacgaaa aatcggaaa atttttggg 
      agtgggctg gtcgagaag gagttagcc aatgagaga 
      gcttga
    mRNA sequence
    • >TC006337 RA
      tgggggaaa acagtcaaa aagtggtgc caactcggt 
      gcgtgaaag tgcgatcga attcgaagc tagttcgaa 
      ccggatttt tgtgaaaga gttttctgt gagaaagtg 
      tgataaaag atgcctctg taagcgcag tgcctcgat 
      ggtagagca aatcaaacc caagactga gttgagcag 
      agatccccg tttgaggcc agatgacca ccgacacgg 
      tgattcccc tggacgagg tcagcgaga gctcgacct 
      tgaccggaa aacaccccc cgaagccga aaatccccg 
      acgggggat ggggctgga tggtggtcc tctcctccc 
      tcatcatgt ccctgatcc aggacggga tcagcttct 
      cgttcggga tcctctaca cggagttcg tggaggagt 
      ttgaagcct ccaagagca caacttcct ggatcggaa 
      gccttttcc tctcagtgc ctctgctga ccggcccga 
      tcatgagtg ccttggttg acaagtatg gctgccggg 
      cgatgacca tcctaggcg gcctggtct cggccactg 
      gattcatcc tgagttatt tcgccacga acgtcatca 
      ccatgtatg tgacgtttg gtgtcattt cgggaatgg 
      ggctaggtc tgacgtata taacagcag tggtgtcaa 
      tcgccttct ggtttgata aaaaaagga acctagcgg 
      tgggcttag gggcgtgtg gtaccggga ttggtactt 
      ttgtctacg cccctttta ccaccatgc tgatttttg 
      agtatggtt ggcggtgga ctgtcatcc tgctcgccg 
      ggactttac tcaacttgt gcgtatgtg gcgccctca 
      tgcgggatc ccgattgga taatcgagc aaaacaagg 
      aaaactcca aactgtcga gcaagtcga gctccctgt 
      ccaatattt cgcacgagt cgaaaaaaa tcgaccttg 
      aagagatac gagagttgt tgaaaaacg gcaaagacg 
      ccgaatata tcttgcaaa ctttggcga cttcgattg 
      atcgcgagg acaaaggac agaaagttg aacatcacc 
      agtcggtac tcaacttgc cgacgttca tccgtcaga 
      acgaaaaag tgccagcag aggtcctcg aacagttgc 
      aagaaaata aaaaactct accgaatta ttgtacaaa 
      attatccca gtttgctcc actgccgca gcacttccg 
      aaaaaggcc tcaataaaa tggaggagg ccataaccc 
      gcatcccag tgaccttct ccctgaagg tgaaaaaaa 
      gcgaccgga aggctgtaa cgcaccagt tctccctcc 
      ccgagaatc ctctggccg ttgagaaga aagtgaaaa 
      aagcggaga cgccatggc tggtaaaac aattcagta 
      ctaccacca cacacccaa attatttta aaaatatga 
      ggttcccta ggcactcgc ttatacacc gcggcgcca 
      ttttgaata aaaataagt acaagttga gggcgtcga 
      gttgtccta acatatacc gagtgtcca tgacgacgc 
      tagtgaaag acgacgatg agccatggt attcgggtc 
      ttgtcaaat tcgttaacc ttttcgacg agtttcact 
      atcttctaa tgtctgtct ctactgtca ttcttttcg 
      cttggttta tgacgcctt atttctact tagtcgacc 
      atatggaag cgtcaggat acaccaaag acgaggctt 
      cctacgtcc tgtcaggga taggcgtgg ccaacacaa 
      tcggaatgg tagtgctcg gttgggccg gcgaccaat 
      cgtggacca acgtctcca aaacgtacg gtgtatgcc 
      tcatcctat gcggggcct gttgctccg ccctctatt 
      tctacacag ccaattaca tcatggtgc acatttttg 
      gcgccctct atggcattt ttctagcta gtagtttct 
      cgtttacgc cgattctgt tggtggaac tgttgccac 
      tagaaacgt ttaccaaag cctacggtt tacaattga 
      tgtgtcaag ggattgggc atttggggg gtccaccgt 
      ttgccgcac tacttgccg atatcacca atagttgga 
      gttacgcct ttcatttgt ctggggtta tgatcgtcc 
      tttccggga tcttgattc tggtgattc cttatacga 
      aaaatcgga aaatttttg ggagtgggc tggtcgaga 
      aggagttag ccaatgaga gagcttgat catgacgga 
      attatgcgt acttaagcg tataaccca agtacttaa 
      tgtaatagt attgtcgtt taataaaaa agtacaatt 
      ttcctcttg taattgttt tcttaaaac gtcaaagtg 
      acagttttg tgactgtca tggtgggac ttgtgcccc 
      tccttgtcg tgtttgtgg ctccgccca ctagcccct 
      cccctgtgg cttacatca tcaataaat tgggctttg 
      tacctccta ctctcatca caagtggcg tcatagtca 
      gcgatactt acgtaataa cccccaact accccgtat 
      ctaccgtgg tagtctccc aagtgtgtg tattacctg 
      ccactaaca tacacaaag ttgataaaa gccacattc 
      gcaactttt gcaaagaca tcaaaacga ttacttcgc 
      tgcgtcaaa taactttac tggaactaa cccaagcct 
      tgattttca cctataagg tcaatgcgg ccgcaattc 
      ctgcagtca agacactga acggctccc tagacttga 
      ccaaaaact gccagttgg tggtaaatt acagatacg 
      atggcttcg tggtttcac atggggtag tacctgtaa 
      gcctgcagg tgacgggtt ctaacatca gactggttg 
      aagagcggc agttttgcg gtggggaca ctgggttac 
      ccgtctatt gcgaaaacg cagttgtgg gaattatgc 
      tgttcccgc aatctaagg acgtttgtt gtccgagtg 
      gctgtatat ttaacacac tactacata tctccattt 
      tctcggaga acgagattc aagacttat taagggaaa 
      aaattgggt tttcttctg gttagttga ggttatatt 
      cccgtgcca gtgttaatt gaatgaaaa aatgtgacc 
      aaataaaat attccgctg ttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY