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Gene information
General information
TC006330 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7294021..7297439
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006330
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01033

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%
    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006330 RA
      MAAAAIRKP VIPKDDSDD DKELQVELK GLVDRITGD 
      ESKLVNVSL DMLKYLIRT STSSMTSVP KPLKYLAPF 
      YGALRNKCD SMKDPLLKK NLSDVVSVL SMGAIDGSV 
      KRDFDCLKY CLQGTMENI GDWGHEYIR QLEIEIVKQ 
      WVMCENNYK TLSPLVKQI MRFNCSHHD EIQGCDLLM 
      EINQMYMLF DYVTRENYK PICLYLASC AKYVDDLES 
      KKILKLISQ FYYLFQEHC RCLVVAIQI KDSALIEKL 
      FNTCTDKIT LYQMAFICA RHLYPIEFN LDDDDVRSI 
      LENSHLSHY FLSFARELD IMEPKSPEE VYKTWLEPV 
      TLRQSVLGE KLDSARQNL ASSFVNGFV NAGFGTDKL 
      LTTDDGNKW IYRNKDVGM LSATAALGL LYLWDVDGG 
      LTPIDKYLY TSDDNIKAG ALLALGIVN CRIRNDCDP 
      ALALLGDYV GSGSEELQV GAVLGIGLA YAGSGRSDV 
      LNLLLPVIQ SSKLVKTLG VASLACGLI SLGRNNNEL 
      SDIILTKMI ESNGLDVLK SPFMLLAGL GVALAYFGT 
      KDAIEVPTI VADVFEEPF RTIFQTMLQ MCAYAGSGD 
      VLIIQELLR LVEEKVEVP ETTETKGSK DKKKNEWDY 
      HMGKAIAVL AVAAISKGE ELGTEMIQR IFGQISRYG 
      EPSVRKAVP LAIALTSVS DPQPTIINV LTKYSHDID 
      DDVACNAIF GLGYVGAGT NNARLAVTL RQLALYHAK 
      NPFQLFMVR IAQGLVHMG KGTMTLNPL HTDRQLLDP 
      VAMAGILIP LVCLLKPHS LILGRCHYL LYSLAAAMQ 
      PRWLLTLDD NLNSVSVTV RVGQAVDTV GKAGDPKTI 
      AGIHTHTTP VLLCANERA EFANGEYES TAPALDGIC 
      VLKKLD
    CDS sequence
    • >TC006330 RA
      atggcagct gcagcaatc cgaaaaccg gtgattccc 
      aaagatgac tcggacgat gacaaggaa ttacaagtg 
      gagctaaag ggtctcgtg gaccgaatt acgggtgac 
      gaatcgaag ctagtgaat gtgtccctc gacatgtta 
      aagtactta atacggacc tcaacctcg tccatgacg 
      tccgtccca aagcccctc aaatacctg gcccctttt 
      tacggggcc ctgcgcaac aaatgcgac tcgatgaag 
      gaccccctc ctgaaaaaa aatctctcc gatgtcgtc 
      tccgtcctg tccatgggg gctatcgac gggagcgtg 
      aaacgcgac ttcgactgt ctcaagtac tgtctccaa 
      ggcacgatg gagaacatc ggcgattgg gggcatgaa 
      tacatccgc cagctggag atcgaaatc gtcaaacag 
      tgggtcatg tgcgagaac aactacaag actctgagc 
      ccccttgtc aagcagatc atgaggttt aactgcagc 
      caccacgac gagatacaa ggctgcgac cttttaatg 
      gaaatcaac cagatgtac atgctcttc gattacgtc 
      acgagggaa aactacaag cccatttgc ttgtaccta 
      gcctcctgt gcaaaatat gtggacgat ctcgagagc 
      aaaaaaata ctgaaactt atttcgcag ttctattat 
      ctttttcag gagcattgt cgctgtttg gtcgtcgcg 
      attcaaatc aaagactcg gctttgatc gagaaactc 
      ttcaatacg tgcactgac aagatcaca ctgtatcaa 
      atggccttc atatgcgcc cgacacctc tacccaatt 
      gaatttaat ctcgacgat gacgacgtg aggagcatt 
      ctcgagaat agtcacttg agccactat tttttaagt 
      tttgcacgt gagctggat ataatggag ccgaaatcg 
      cccgaagag gtgtacaaa acctggctt gagcccgta 
      acgctgcgt cagagcgtc ctcggcgag aaactggac 
      agtgcccgt caaaatctg gcctcgtcc ttcgtcaac 
      ggtttcgtc aatgctggt tttggcact gataaactt 
      ttgacaacc gacgatggc aataaatgg atttaccga 
      aacaaagat gttggcatg ttaagtgcc actgcagct 
      ctgggttta ctatactta tgggacgta gatggcggt 
      ttgacccca attgataaa tatctctac accagtgat 
      gataatatc aaagctggc gcgttacta gcccttggt 
      attgtcaat tgtcgcatt cgaaacgac tgcgacccg 
      gcccttgcc ctactgggc gattacgtg gggagtggc 
      agtgaggag ttgcaagtt ggggcggtg ctaggcatc 
      ggattggcg tacgccggg agtgggcgg agcgacgta 
      ttgaatctc ctcctaccg gtgatacag agctcgaaa 
      ttggtcaaa actttgggc gtggcgagt cttgcatgc 
      ggtttgatc agtctcggg agaaataat aacgaatta 
      tcggacatc atcttgacg aaaatgatt gaatcgaac 
      ggattggac gttctgaaa agtcccttc atgttattg 
      gctggtttg ggcgtggca cttgcatat ttcggaacc 
      aaggatgcg attgaggtt ccgacgatt gtggcggat 
      gtgtttgag gagccgttt cggaccatc ttccagacc 
      atgctacaa atgtgcgct tacgccgga agtggggat 
      gtgttaata atccaggaa ttgttgcga ttggtcgag 
      gaaaaggtc gaagttccc gaaacgacc gaaacgaaa 
      ggttcgaaa gataagaaa aagaacgaa tgggattat 
      catatgggg aaagcgata gctgttttg gccgtggcc 
      gccatttcc aagggtgag gagctcggg accgaaatg 
      attcaaaga attttcggt caaatatca cgttatggc 
      gaaccttcg gtccgcaaa gccgtccca ttggctatc 
      gccttgact tcggttagc gatccccag cccactata 
      atcaacgtt ttgacgaaa tattcacac gatattgac 
      gacgacgtt gcttgcaac gccattttt gggctgggt 
      tatgtgggg gcgggcact aacaatgcc cgccttgcg 
      gtcactcta cgccaacta gccttatac catgccaag 
      aacccgttc caattgttt atggtaaga attgcgcag 
      ggtcttgtc catatgggt aaaggtacc atgactttg 
      aaccccttg cacaccgat cgtcagttg ctagacccg 
      gttgcaatg gcggggatt ttgatcccg ttagtgtgc 
      ctacttaag ccgcattcg ttgattttg ggccgatgt 
      cattaccta ctctatagc ttagcggcg gccatgcaa 
      ccaagatgg ctgctcaca cttgacgat aatttgaat 
      tctgtctca gtgacagtt cgtgtgggt caggctgtg 
      gatacggta ggaaaggca ggtgatcct aaaaccatt 
      gcggggatt cacacgcat acgacacca gttttgttg 
      tgcgctaac gaaagggcg gagtttgca aatggggag 
      tacgaatca acagcgccc gcccttgac ggcatttgc 
      gttttaaaa aagcttgac taa
    mRNA sequence
    • >TC006330 RA
      gtccattaa tcgtaaaaa catcggtaa tttgagcaa 
      tttcttcaa tttctggac accaacaaa cactaaatt 
      ggggctatt tacccaaaa gacgcactt atgaaacaa 
      gcggcaaaa actaaataa ggttagggc ggaaacgga 
      aacgccaaa acgtcaaat ttaaatttt tgcatgtca 
      tttgaaacg gtcgctcct tttgcccat ttttgcaaa 
      aattcgccg aaatggcag ctgcagcaa tccgaaaac 
      cggtgattc ccaaagatg actcggacg atgacaagg 
      aattacaag tggagctaa agggtctcg tggaccgaa 
      ttacgggtg acgaatcga agctagtga atgtgtccc 
      tcgacatgt taaagtact taatacgga cctcaacct 
      cgtccatga cgtccgtcc caaagcccc tcaaatacc 
      tggcccctt tttacgggg ccctgcgca acaaatgcg 
      actcgatga aggaccccc tcctgaaaa aaaatctct 
      ccgatgtcg tctccgtcc tgtccatgg gggctatcg 
      acgggagcg tgaaacgcg acttcgact gtctcaagt 
      actgtctcc aaggcacga tggagaaca tcggcgatt 
      gggggcatg aatacatcc gccagctgg agatcgaaa 
      tcgtcaaac agtgggtca tgtgcgaga acaactaca 
      agactctga gcccccttg tcaagcaga tcatgaggt 
      ttaactgca gccaccacg acgagatac aaggctgcg 
      accttttaa tggaaatca accagatgt acatgctct 
      tcgattacg tcacgaggg aaaactaca agcccattt 
      gcttgtacc tagcctcct gtgcaaaat atgtggacg 
      atctcgaga gcaaaaaaa tactgaaac ttatttcgc 
      agttctatt atctttttc aggagcatt gtcgctgtt 
      tggtcgtcg cgattcaaa tcaaagact cggctttga 
      tcgagaaac tcttcaata cgtgcactg acaagatca 
      cactgtatc aaatggcct tcatatgcg cccgacacc 
      tctacccaa ttgaattta atctcgacg atgacgacg 
      tgaggagca ttctcgaga atagtcact tgagccact 
      attttttaa gttttgcac gtgagctgg atataatgg 
      agccgaaat cgcccgaag aggtgtaca aaacctggc 
      ttgagcccg taacgctgc gtcagagcg tcctcggcg 
      agaaactgg acagtgccc gtcaaaatc tggcctcgt 
      ccttcgtca acggtttcg tcaatgctg gttttggca 
      ctgataaac ttttgacaa ccgacgatg gcaataaat 
      ggatttacc gaaacaaag atgttggca tgttaagtg 
      ccactgcag ctctgggtt tactatact tatgggacg 
      tagatggcg gtttgaccc caattgata aatatctct 
      acaccagtg atgataata tcaaagctg gcgcgttac 
      tagcccttg gtattgtca attgtcgca ttcgaaacg 
      actgcgacc cggcccttg ccctactgg gcgattacg 
      tggggagtg gcagtgagg agttgcaag ttggggcgg 
      tgctaggca tcggattgg cgtacgccg ggagtgggc 
      ggagcgacg tattgaatc tcctcctac cggtgatac 
      agagctcga aattggtca aaactttgg gcgtggcga 
      gtcttgcat gcggtttga tcagtctcg ggagaaata 
      ataacgaat tatcggaca tcatcttga cgaaaatga 
      ttgaatcga acggattgg acgttctga aaagtccct 
      tcatgttat tggctggtt tgggcgtgg cacttgcat 
      atttcggaa ccaaggatg cgattgagg ttccgacga 
      ttgtggcgg atgtgtttg aggagccgt ttcggacca 
      tcttccaga ccatgctac aaatgtgcg cttacgccg 
      gaagtgggg atgtgttaa taatccagg aattgttgc 
      gattggtcg aggaaaagg tcgaagttc ccgaaacga 
      ccgaaacga aaggttcga aagataaga aaaagaacg 
      aatgggatt atcatatgg ggaaagcga tagctgttt 
      tggccgtgg ccgccattt ccaagggtg aggagctcg 
      ggaccgaaa tgattcaaa gaattttcg gtcaaatat 
      cacgttatg gcgaacctt cggtccgca aagccgtcc 
      cattggcta tcgccttga cttcggtta gcgatcccc 
      agcccacta taatcaacg ttttgacga aatattcac 
      acgatattg acgacgacg ttgcttgca acgccattt 
      ttgggctgg gttatgtgg gggcgggca ctaacaatg 
      cccgccttg cggtcactc tacgccaac tagccttat 
      accatgcca agaacccgt tccaattgt ttatggtaa 
      gaattgcgc agggtcttg tccatatgg gtaaaggta 
      ccatgactt tgaacccct tgcacaccg atcgtcagt 
      tgctagacc cggttgcaa tggcgggga ttttgatcc 
      cgttagtgt gcctactta agccgcatt cgttgattt 
      tgggccgat gtcattacc tactctata gcttagcgg 
      cggccatgc aaccaagat ggctgctca cacttgacg 
      ataatttga attctgtct cagtgacag ttcgtgtgg 
      gtcaggctg tggatacgg taggaaagg caggtgatc 
      ctaaaacca ttgcgggga ttcacacgc atacgacac 
      cagttttgt tgtgcgcta acgaaaggg cggagtttg 
      caaatgggg agtacgaat caacagcgc ccgcccttg 
      acggcattt gcgttttaa aaaagcttg actaatcta 
      tgtgtttta ttttattgt atttgtgtt taataaagt 
      ttgctttac gat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY