Status
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Gene information
General information
TC034041
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006322,TC006321
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7195891..7216639
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034041
    iBeetle screen iB_01032

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034041 RA
      MQAALLIRE KQRLRRKKL RKGRAELPS HIATREIAP 
      FPQYPPGFM CSQGRAAWQ ERERGRRCS LSEEARQSR 
      IKQSEAHHR WMKRNRIHD STYGTGSEE DDFFSVYHQ 
      SAAANALLY VGLGTTAIG SVIFFVGTG EKGFKTLEL 
      RLIGPTLIA CGLLCCLIR VLLCACPST CLRRRKKTR 
      LKATCPHTS RYPKSRDHV DSAALLSKN KKKVSIVPP 
      SHPQPSTST QEFKEQKTQ EKITLPQIN LPEEEAKMA 
      RDDSVIELQ NLELIYDIQ SVSSNDSDD NVTTVVETR 
      TTNDLETCL SVVIERSDN SESSDGVKN QESGDKQTH 
      SGIVLSPLQ LGQ
    • >TC034041 RB
      MSTAASAGM QAALLIREK QRLRRKKLR KGRAELPSH 
      IATREIAPF PQYPPGFMC SQGRAAWQE RERGRRCSL 
      SEEARQSRI KQSEAHHRW MKRNRIHDS TYGTGSEED 
      DFFSVYHQS AAANALLYV GLGTTAIGS VIFFVGTGE 
      KGFKTLELR LIGPTLIAC GLLCCLIRV LLCACPSTC 
      LRRRKKTRL KATCPHTSR YPKSRDHVD SAALLSKNK 
      KKVSIVPPS HPQPSTSTQ EFKEQKTQE KITLPQINL 
      PEEEAKMAR DDSVIELQN LELIYDIQS VSSNDSDDN 
      VTTVVETRT TNDLETCLS VVIERSDNS ESSDGVKNQ 
      ESGDKQTHS GIVLSPLQL GQ
    • >TC034041 RC
      MYAAAGGAS LASRQARQR QRQKQLQQL KNQKELKPH 
      QPKQYHNYT DDGGPKLSR VERGALRPP QGPHERRHS 
      ASNYHLKEP PKSRLKESP SISIPVQSR PSLVENHVS 
      ASPSLQLPQ IFIPEGDGR LERRCSFVR QVEEMEKCP 
      EGGLLDKCN HLCNFEIKD RIWDNKYEF ERDLYGSLD 
      YPFSECSQG RAAWQERER GRRCSLSEE ARQSRIKQS 
      EAHHRWMKR NRIHDSTYG TGSEEDDFF SVYHQSAAA 
      NALLYVGLG TTAIGSVIF FVGTGEKGF KTLELRLIG 
      PTLIACGLL CCLIRVLLC ACPSTCLRR RKKTRLKAT 
      CPHTSRYPK SRDHVDSAA LLSKNKKKV SIVPPSHPQ 
      PSTSTQEFK EQKTQEKIT LPQINLPEE EAKMARDDS 
      VIELQNLEL IYDIQSVSS NDSDDNVTT VVETRTTND 
      LETCLSVVI ERSDNSESS DGVKNQESG DKQTHSGIV 
      LSPLQLGQ
    CDS sequence
    • >TC034041 RA
      atgcaagcc gccttactc attagagag aaacagcgt 
      ctccgcagg aagaagttg aggaaggga cgggccgaa 
      ctgcccagt catatcgcg accagagag atcgcgccc 
      ttcccacaa tatccgccc ggatttatg tgttcacag 
      ggccgtgcg gcatggcaa gagagggaa agggggaga 
      cgctgttca ctctccgag gaagcgcgg caatcacga 
      atcaaacaa tcggaggct catcaccga tggatgaaa 
      cggaacaga atccacgac tccacctac ggtaccggt 
      tcagaagaa gacgacttc ttcagcgtt taccatcaa 
      agtgccgcc gccaacgct ctcctgtac gtcggtctg 
      ggcaccaca gcgatcggc agcgtgatc ttcttcgtc 
      ggcaccggc gagaaaggc ttcaaaacc ctcgaactc 
      cgcctgatc ggccccacg ctgatcgct tgtggcctc 
      ctctgctgc ctaatccgc gtcctgctc tgcgcctgc 
      ccttccacg tgtctccgg cgccggaag aagacccgg 
      ctgaaggcc acctgtcct cacacttcc agatacccg 
      aaatcgcgc gaccacgtc gacagcgcc gccctcctc 
      tccaaaaac aagaaaaag gtctcgatc gtgcccccc 
      agccacccc cagcccagc actagcacg caagagttc 
      aaagaacag aaaacgcaa gagaagatc actctgcca 
      cagatcaat cttcccgaa gaggaggcg aagatggcg 
      cgcgacgat tccgtcatc gaactgcag aatctcgag 
      ctaatctac gacatacag agtgtttcg agtaatgac 
      agtgatgat aacgtaact acagttgtc gaaacacgg 
      acgaccaat gaccttgag acttgtcta agtgttgtc 
      atcgaacgg agtgacaat agtgagagc agtgatggg 
      gtcaaaaat caagaaagc ggtgataaa caaacgcat 
      agcggaatt gtgctcagc ccactacaa ttgggacag 
      taa
    • >TC034041 RB
      atgagcaca gcagcatca gcaggaatg caagccgcc 
      ttactcatt agagagaaa cagcgtctc cgcaggaag 
      aagttgagg aagggacgg gccgaactg cccagtcat 
      atcgcgacc agagagatc gcgcccttc ccacaatat 
      ccgcccgga tttatgtgt tcacagggc cgtgcggca 
      tggcaagag agggaaagg gggagacgc tgttcactc 
      tccgaggaa gcgcggcaa tcacgaatc aaacaatcg 
      gaggctcat caccgatgg atgaaacgg aacagaatc 
      cacgactcc acctacggt accggttca gaagaagac 
      gacttcttc agcgtttac catcaaagt gccgccgcc 
      aacgctctc ctgtacgtc ggtctgggc accacagcg 
      atcggcagc gtgatcttc ttcgtcggc accggcgag 
      aaaggcttc aaaaccctc gaactccgc ctgatcggc 
      cccacgctg atcgcttgt ggcctcctc tgctgccta 
      atccgcgtc ctgctctgc gcctgccct tccacgtgt 
      ctccggcgc cggaagaag acccggctg aaggccacc 
      tgtcctcac acttccaga tacccgaaa tcgcgcgac 
      cacgtcgac agcgccgcc ctcctctcc aaaaacaag 
      aaaaaggtc tcgatcgtg ccccccagc cacccccag 
      cccagcact agcacgcaa gagttcaaa gaacagaaa 
      acgcaagag aagatcact ctgccacag atcaatctt 
      cccgaagag gaggcgaag atggcgcgc gacgattcc 
      gtcatcgaa ctgcagaat ctcgagcta atctacgac 
      atacagagt gtttcgagt aatgacagt gatgataac 
      gtaactaca gttgtcgaa acacggacg accaatgac 
      cttgagact tgtctaagt gttgtcatc gaacggagt 
      gacaatagt gagagcagt gatggggtc aaaaatcaa 
      gaaagcggt gataaacaa acgcatagc ggaattgtg 
      ctcagccca ctacaattg ggacagtaa
    • >TC034041 RC
      atgtacgcg gcagcaggg ggcgcttcg ctcgcctcc 
      cgccaggcg cgccagcgg cagcgccag aagcagctc 
      cagcagctc aagaaccag aaggagctc aagccgcac 
      cagccgaag cagtaccac aactacacg gacgatggg 
      gggcccaag ctgtcgcgg gtggagcgc ggggcgctg 
      cgcccgccg caggggccc cacgagcgc cgccactcc 
      gcctccaac taccacctc aaggagccg cccaagagc 
      cggctcaag gagagcccc tccatctcg atcccggtc 
      cagagtcgg ccctccctc gtggagaac cacgtctcg 
      gcctcgccc tcgctccag ctgccccag atcttcatt 
      cccgaaggg gacggccgg ctggagcgg aggtgcagc 
      ttcgtcaga caggtggag gagatggag aagtgtccc 
      gagggaggg ctgctggat aagtgcaac catttgtgc 
      aatttcgag atcaaggac aggatctgg gacaacaag 
      tacgagttt gagagggat ctctatggc agtctggat 
      tatcccttt tccgagtgt tcacagggc cgtgcggca 
      tggcaagag agggaaagg gggagacgc tgttcactc 
      tccgaggaa gcgcggcaa tcacgaatc aaacaatcg 
      gaggctcat caccgatgg atgaaacgg aacagaatc 
      cacgactcc acctacggt accggttca gaagaagac 
      gacttcttc agcgtttac catcaaagt gccgccgcc 
      aacgctctc ctgtacgtc ggtctgggc accacagcg 
      atcggcagc gtgatcttc ttcgtcggc accggcgag 
      aaaggcttc aaaaccctc gaactccgc ctgatcggc 
      cccacgctg atcgcttgt ggcctcctc tgctgccta 
      atccgcgtc ctgctctgc gcctgccct tccacgtgt 
      ctccggcgc cggaagaag acccggctg aaggccacc 
      tgtcctcac acttccaga tacccgaaa tcgcgcgac 
      cacgtcgac agcgccgcc ctcctctcc aaaaacaag 
      aaaaaggtc tcgatcgtg ccccccagc cacccccag 
      cccagcact agcacgcaa gagttcaaa gaacagaaa 
      acgcaagag aagatcact ctgccacag atcaatctt 
      cccgaagag gaggcgaag atggcgcgc gacgattcc 
      gtcatcgaa ctgcagaat ctcgagcta atctacgac 
      atacagagt gtttcgagt aatgacagt gatgataac 
      gtaactaca gttgtcgaa acacggacg accaatgac 
      cttgagact tgtctaagt gttgtcatc gaacggagt 
      gacaatagt gagagcagt gatggggtc aaaaatcaa 
      gaaagcggt gataaacaa acgcatagc ggaattgtg 
      ctcagccca ctacaattg ggacagtaa
    mRNA sequence
    • >TC034041 RA
      gtggaaaat gagcaattg aagcgtcga aaaatgctg 
      gaaatgcgc aaccgtttc gatccgggg taacgtcga 
      ggaccaagt acgatgcaa aaaaaccaa ggggccatc 
      aacatatca accaaatgt agagaaaaa gaatcgacc 
      gttgtagca tcggaacca atctttgtg caaatattt 
      ttggagaat tttgccctt ccacgcttg cttcgtccg 
      actgccact tatattata cacgcctaa ctctacgct 
      tatgctgaa caatgcctt tctataata tcgtctata 
      tacagtgtg atagtgcta tttttgtat gtgatcttt 
      tcggcgagc gttgatttg gtgacgatg atcgggtgt 
      tcggccgcg gtcattggt gacaacgtt gcatgagca 
      cagcagcat cagcaggaa tgcaagccg ccttactca 
      ttagagaga aacagcgtc tccgcagga agaagttga 
      ggaagggac gggccgaac tgcccagtc atatcgcga 
      ccagagaga tcgcgccct tcccacaat atccgcccg 
      gatttatgt gttcacagg gccgtgcgg catggcaag 
      agagggaaa gggggagac gctgttcac tctccgagg 
      aagcgcggc aatcacgaa tcaaacaat cggaggctc 
      atcaccgat ggatgaaac ggaacagaa tccacgact 
      ccacctacg gtaccggtt cagaagaag acgacttct 
      tcagcgttt accatcaaa gtgccgccg ccaacgctc 
      tcctgtacg tcggtctgg gcaccacag cgatcggca 
      gcgtgatct tcttcgtcg gcaccggcg agaaaggct 
      tcaaaaccc tcgaactcc gcctgatcg gccccacgc 
      tgatcgctt gtggcctcc tctgctgcc taatccgcg 
      tcctgctct gcgcctgcc cttccacgt gtctccggc 
      gccggaaga agacccggc tgaaggcca cctgtcctc 
      acacttcca gatacccga aatcgcgcg accacgtcg 
      acagcgccg ccctcctct ccaaaaaca agaaaaagg 
      tctcgatcg tgcccccca gccaccccc agcccagca 
      ctagcacgc aagagttca aagaacaga aaacgcaag 
      agaagatca ctctgccac agatcaatc ttcccgaag 
      aggaggcga agatggcgc gcgacgatt ccgtcatcg 
      aactgcaga atctcgagc taatctacg acatacaga 
      gtgtttcga gtaatgaca gtgatgata acgtaacta 
      cagttgtcg aaacacgga cgaccaatg accttgaga 
      cttgtctaa gtgttgtca tcgaacgga gtgacaata 
      gtgagagca gtgatgggg tcaaaaatc aagaaagcg 
      gtgataaac aaacgcata gcggaattg tgctcagcc 
      cactacaat tgggacagt aattcgttg ttgtagata 
      cgaaggcgg ctagagggc gccatcaaa actaagtaa 
      aagcgccac ctggcgtcc aaattcaaa atgtatttt 
      tcaattcac aaattcaca agtacagtt ccatcccag 
      gtgtggctt ttatgatca tctgatcaa cgaaacgac 
      agggatttg tttcccgtt gttgacaat acttgttga 
      atcaaaatc atggcgtga tcaggcgtt tcttgatca 
      ggtgacgca aaaatcaaa cgttgcgcc ctctgccgt 
      cgcaactgt tagtacgga attgatcaa aataaaaat 
      caagtcgtg ttgtaatta ttttacgtt gcgtcctta 
      cgtgcaacg tgaacgtat taagtaaag taatatttg 
      ctaaaatta ttataacct agcgtgcct gtattttga 
      tcctaacat ttgctttat cacgttttt tgattagga 
      gattcggat ttttacagc aatattggc ctaagttgg 
      accttccga tgcacaaca atcaaatga gtttttgat 
      cgcgtgatc aattttcgc aatcattcg acaaataaa 
      tactttgca aattttcga ttttatatg gcggccgta 
      gaaatgtaa cctatcaac gtaccatta tgatatttt 
      gattagatt tgagccgag ggtacggga gtaaccttg 
      gcgaccgtt gtcaacaaa attgtgttt tgtttttac 
      caaaatggc ggaattgcg ttcgacaca ccctcagag 
      tgtacatca cgccgcctc cttgatgaa tcgagaact 
      tccttcttg gacggctta tttcccagt acaattttt 
      ctattttta agttgtaat tgattagta agccgcgag 
      gtgtcgccc tgtggtgta aaagaacgg agttaccac 
      tccatcgag gaggccgtg gtacattat aaacttgct 
      atatacata gaaaatata aaaatgtac gtatatgta 
      tcatattcg agaatcatg tataaatca atttttata 
      catgatttg ttgagtaaa aacctataa taaagttat 
      atagaaaaa
    • >TC034041 RB
      gtggaaaat gagcaattg aagcgtcga aaaatgctg 
      gaaatgcgc aaccgtttc gatccgggg taacgtcga 
      ggaccaagt acgatgcaa aaaaaccaa ggggccatc 
      aacatatca accaaatgt agagaaaaa gaatcgacc 
      gttgtagca tcggaacca atctttgtg caaatattt 
      ttggagaat tttgccctt ccacgcttg cttcgtccg 
      actgccact tatattata cacgcctaa ctctacgct 
      tatgctgaa caatgcctt tctataata tcgtctata 
      tacagtgtg atagtgcta tttttgtat gtgatcttt 
      tcggcgagc gttgatttg gtgacgatg atcgggtgt 
      tcggccgcg gtcattggt gacaacgtt gcatgagca 
      cagcagcat cagcaggaa tgcaagccg ccttactca 
      ttagagaga aacagcgtc tccgcagga agaagttga 
      ggaagggac gggccgaac tgcccagtc atatcgcga 
      ccagagaga tcgcgccct tcccacaat atccgcccg 
      gatttatgt gttcacagg gccgtgcgg catggcaag 
      agagggaaa gggggagac gctgttcac tctccgagg 
      aagcgcggc aatcacgaa tcaaacaat cggaggctc 
      atcaccgat ggatgaaac ggaacagaa tccacgact 
      ccacctacg gtaccggtt cagaagaag acgacttct 
      tcagcgttt accatcaaa gtgccgccg ccaacgctc 
      tcctgtacg tcggtctgg gcaccacag cgatcggca 
      gcgtgatct tcttcgtcg gcaccggcg agaaaggct 
      tcaaaaccc tcgaactcc gcctgatcg gccccacgc 
      tgatcgctt gtggcctcc tctgctgcc taatccgcg 
      tcctgctct gcgcctgcc cttccacgt gtctccggc 
      gccggaaga agacccggc tgaaggcca cctgtcctc 
      acacttcca gatacccga aatcgcgcg accacgtcg 
      acagcgccg ccctcctct ccaaaaaca agaaaaagg 
      tctcgatcg tgcccccca gccaccccc agcccagca 
      ctagcacgc aagagttca aagaacaga aaacgcaag 
      agaagatca ctctgccac agatcaatc ttcccgaag 
      aggaggcga agatggcgc gcgacgatt ccgtcatcg 
      aactgcaga atctcgagc taatctacg acatacaga 
      gtgtttcga gtaatgaca gtgatgata acgtaacta 
      cagttgtcg aaacacgga cgaccaatg accttgaga 
      cttgtctaa gtgttgtca tcgaacgga gtgacaata 
      gtgagagca gtgatgggg tcaaaaatc aagaaagcg 
      gtgataaac aaacgcata gcggaattg tgctcagcc 
      cactacaat tgggacagt aattcgttg ttgtagata 
      cgaaggcgg ctagagggc gccatcaaa actaagtaa 
      aagcgccac ctggcgtcc aaattcaaa atgtatttt 
      tcaattcac aaattcaca agtacagtt ccatcccag 
      gtgtggctt ttatgatca tctgatcaa cgaaacgac 
      agggatttg tttcccgtt gttgacaat acttgttga 
      atcaaaatc atggcgtga tcaggcgtt tcttgatca 
      ggtgacgca aaaatcaaa cgttgcgcc ctctgccgt 
      cgcaactgt tagtacgga attgatcaa aataaaaat 
      caagtcgtg ttgtaatta ttttacgtt gcgtcctta 
      cgtgcaacg tgaacgtat taagtaaag taatatttg 
      ctaaaatta ttataacct agcgtgcct gtattttga 
      tcctaacat ttgctttat cacgttttt tgattagga 
      gattcggat ttttacagc aatattggc ctaagttgg 
      accttccga tgcacaaca atcaaatga gtttttgat 
      cgcgtgatc aattttcgc aatcattcg acaaataaa 
      tactttgca aattttcga ttttatatg gcggccgta 
      gaaatgtaa cctatcaac gtaccatta tgatatttt 
      gattagatt tgagccgag ggtacggga gtaaccttg 
      gcgaccgtt gtcaacaaa attgtgttt tgtttttac 
      caaaatggc ggaattgcg ttcgacaca ccctcagag 
      tgtacatca cgccgcctc cttgatgaa tcgagaact 
      tccttcttg gacggctta tttcccagt acaattttt 
      ctattttta agttgtaat tgattagta agccgcgag 
      gtgtcgccc tgtggtgta aaagaacgg agttaccac 
      tccatcgag gaggccgtg gtacattat aaacttgct 
      atatacata gaaaatata aaaatgtac gtatatgta 
      tcatattcg agaatcatg tataaatca atttttata 
      catgatttg ttgagtaaa aacctataa taaagttat 
      atagaaaaa
    • >TC034041 RC
      tgataaata tctacgttt tcgtgaacg ataaatatt 
      caacggacc gacggacgg ctgcttgga aaacggcgt 
      cgtcatgat taccttcag tcgaatttc accttcctt 
      tgttttcca ttttccata atcgtaaca atggattta 
      aattacccg cttgattta gccccgcct attatttga 
      ttcgttcca ataaaatca ggcctcccc tcctctccg 
      aacgatgtg ttcaattct aataggccg agaggagaa 
      aacaatcca aaagcaata cgaaacggt tggctctac 
      gaaactaat aaaagacaa tatcaattt aaaattagt 
      gattactgt aacataaat aaaccgaga agttgaagc 
      ctaatgtta gttctaaac gccgaatta tttgttatt 
      tgttatacg agttagtga aatgtttag cctagtccc 
      cgaaacgat attttgtaa acgaatgaa agtattttg 
      tagtaattt tttcgaacg atgttgtat ttatcggtg 
      aatcaaaag gtgctccgg cactcgaaa gtcaaactg 
      aagccccag ttccttccc ccgcccctc ccgggcccc 
      gcccccggc cgtcattaa gaccatgta cgcggcagc 
      agggggcgc ttcgctcgc ctcccgcca ggcgcgcca 
      gcggcagcg ccagaagca gctccagca gctcaagaa 
      ccagaagga gctcaagcc gcaccagcc gaagcagta 
      ccacaacta cacggacga tggggggcc caagctgtc 
      gcgggtgga gcgcggggc gctgcgccc gccgcaggg 
      gccccacga gcgccgcca ctccgcctc caactacca 
      cctcaagga gccgcccaa gagccggct caaggagag 
      cccctccat ctcgatccc ggtccagag tcggccctc 
      cctcgtgga gaaccacgt ctcggcctc gccctcgct 
      ccagctgcc ccagatctt cattcccga aggggacgg 
      ccggctgga gcggaggtg cagcttcgt cagacaggt 
      ggaggagat ggagaagtg tcccgaggg agggctgct 
      ggataagtg caaccattt gtgcaattt cgagatcaa 
      ggacaggat ctgggacaa caagtacga gtttgagag 
      ggatctcta tggcagtct ggattatcc cttttccga 
      gtgttcaca gggccgtgc ggcatggca agagaggga 
      aagggggag acgctgttc actctccga ggaagcgcg 
      gcaatcacg aatcaaaca atcggaggc tcatcaccg 
      atggatgaa acggaacag aatccacga ctccaccta 
      cggtaccgg ttcagaaga agacgactt cttcagcgt 
      ttaccatca aagtgccgc cgccaacgc tctcctgta 
      cgtcggtct gggcaccac agcgatcgg cagcgtgat 
      cttcttcgt cggcaccgg cgagaaagg cttcaaaac 
      cctcgaact ccgcctgat cggccccac gctgatcgc 
      ttgtggcct cctctgctg cctaatccg cgtcctgct 
      ctgcgcctg cccttccac gtgtctccg gcgccggaa 
      gaagacccg gctgaaggc cacctgtcc tcacacttc 
      cagataccc gaaatcgcg cgaccacgt cgacagcgc 
      cgccctcct ctccaaaaa caagaaaaa ggtctcgat 
      cgtgccccc cagccaccc ccagcccag cactagcac 
      gcaagagtt caaagaaca gaaaacgca agagaagat 
      cactctgcc acagatcaa tcttcccga agaggaggc 
      gaagatggc gcgcgacga ttccgtcat cgaactgca 
      gaatctcga gctaatcta cgacataca gagtgtttc 
      gagtaatga cagtgatga taacgtaac tacagttgt 
      cgaaacacg gacgaccaa tgaccttga gacttgtct 
      aagtgttgt catcgaacg gagtgacaa tagtgagag 
      cagtgatgg ggtcaaaaa tcaagaaag cggtgataa 
      acaaacgca tagcggaat tgtgctcag cccactaca 
      attgggaca gtaattcgt tgttgtaga tacgaaggc 
      ggctagagg gcgccatca aaactaagt aaaagcgcc 
      acctggcgt ccaaattca aaatgtatt tttcaattc 
      acaaattca caagtacag ttccatccc aggtgtggc 
      ttttatgat catctgatc aacgaaacg acagggatt 
      tgtttcccg ttgttgaca atacttgtt gaatcaaaa 
      tcatggcgt gatcaggcg tttcttgat caggtgacg 
      caaaaatca aacgttgcg ccctctgcc gtcgcaact 
      gttagtacg gaattgatc aaaataaaa atcaagtcg 
      tgttgtaat tattttacg ttgcgtcct tacgtgcaa 
      cgtgaacgt attaagtaa agtaatatt tgctaaaat 
      tattataac ctagcgtgc ctgtatttt gatcctaac 
      atttgcttt atcacgttt tttgattag gagattcgg 
      atttttaca gcaatattg gcctaagtt ggaccttcc 
      gatgcacaa caatcaaat gagtttttg atcgcgtga 
      tcaattttc gcaatcatt cgacaaata aatactttg 
      caaattttc gattttata tggcggccg tagaaatgt 
      aacctatca acgtaccat tatgatatt ttgattaga 
      tttgagccg agggtacgg gagtaacct tggcgaccg 
      ttgtcaaca aaattgtgt tttgttttt accaaaatg 
      gcggaattg cgttcgaca caccctcag agtgtacat 
      cacgccgcc tccttgatg aatcgagaa cttccttct 
      tggacggct tatttccca gtacaattt ttctatttt 
      taagttgta attgattag taagccgcg aggtgtcgc 
      cctgtggtg taaaagaac ggagttacc actccatcg 
      aggaggccg tggtacatt ataaacttg ctatataca 
      tagaaaata taaaaatgt acgtatatg tatcatatt 
      cgagaatca tgtataaat caattttta tacatgatt 
      tgttgagta aaaacctat aataaagtt atatagaaa 
      aa
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