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Gene information
General information
TC006308 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid56: 3452..5840
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006308
    iBeetle screen iB_01031
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
    appendages partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen partially remaining - Penetrance (percentage of animals/eggs on slide): less than 30%
    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 37.5% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006308 RA
      MATKGFKVT DSERKSRVG IAAKTLDDL KRKTVEKFK 
      LELPPDEIF LQTQDGTLV ENDEYFQTL HAQTLLIWV 
      KNGEKAPTD AEILYKTIR EVNDEYLSA GEKVQEFFT 
      EKMKNKVFK LAEVLRGID GDKAKFSLK CDHPEWFEG 
      LDTNAKTKE DYMFRRAQD RIRTYFYKT REELLKEPT 
      LPPNRLRSL VDELQNRLK LSKFNGHFF DRSDNKSLC 
      DERGEFTCQ GRWNKDMCL YSPQHRINP YESREGRII 
      FQTWNLDHV IERSRAVIP GIVKALKDA ECTIVNDRE 
      ASVDVKAIY NDLFTLHNL KFVHIVCHD KGRHSTKKA 
      GPYLLY
    CDS sequence
    • >TC006308 RA
      atggccacc aagggcttc aaagtcacc gattccgaa 
      cgcaagtca cgtgtgggc attgccgcc aagaccctc 
      gacgacttg aaacgcaaa accgtggaa aaattcaag 
      ttggagctg ccccccgac gagattttc ctccagacg 
      caggacggc actctggtg gaaaacgac gagtatttt 
      cagaccttg cacgcgcaa acgctcctc atctgggtg 
      aaaaacggc gaaaaggcc cccactgat gcggaaatt 
      ttgtacaaa accatccgc gaagtcaac gacgagtat 
      ctgagcgcg ggggaaaaa gtccaggag tttttcacc 
      gagaaaatg aaaaacaaa gttttcaag ttggccgag 
      gtgctccgg gggatcgac ggcgacaaa gccaagttc 
      agtctcaag tgcgaccac cccgagtgg ttcgaaggc 
      cttgacacc aatgcaaaa accaaagag gactatatg 
      tttaggagg gcgcaggac cggattcgg acctatttc 
      tacaaaacc cgagaggaa ctgttgaag gagcccacg 
      ctgccccca aaccggttg cgaagcctc gtcgacgag 
      cttcaaaac cgacttaaa ctgtccaag ttcaatgga 
      catttcttc gatcgaagc gacaacaag agcttgtgc 
      gacgagaga ggcgagttc acctgtcag ggcaggtgg 
      aacaaagac atgtgtctc tacagcccc caacatcgc 
      attaatcct tacgaaagc agggagggg aggattatc 
      ttccagacg tggaatttg gaccacgtg attgaacgc 
      tcgagggcg gttataccc gggatcgtg aaggctttg 
      aaagacgcc gagtgcacg atcgttaac gatcgcgag 
      gcttcggtc gatgtcaaa gccatctat aacgatttg 
      ttcactttg cacaacctg aagtttgta catattgtg 
      tgtcatgat aagggtagg catagcacg aaaaaggca 
      gggccgtat ctgctttat taa
    mRNA sequence
    • >TC006308 RA
      ttgtgtttt ttaattcac gtgtcgatt tgagttatc 
      catgcgttt cgcatcgtt tttgttaaa attgcgacg 
      tcgggggca atttgcgtc ttaattgtg gaaatgtgg 
      aaaatcgcg ttgggtcaa gtgacatgc gcactttcc 
      ccggaaaag ggaaattga cactgacat tggcctagt 
      tgctttgat tttttaggt tgcctgcat ttaagtgca 
      gctgcaacc aggaaaacc tcagtcggt gctcgagcg 
      cagagttgg ttgcatcaa accgggcgc gatggccac 
      caagggctt caaagtcac cgattccga acgcaagtc 
      acgtgtggg cattgccgc caagaccct cgacgactt 
      gaaacgcaa aaccgtgga aaaattcaa gttggagct 
      gccccccga cgagatttt cctccagac gcaggacgg 
      cactctggt ggaaaacga cgagtattt tcagacctt 
      gcacgcgca aacgctcct catctgggt gaaaaacgg 
      cgaaaaggc ccccactga tgcggaaat tttgtacaa 
      aaccatccg cgaagtcaa cgacgagta tctgagcgc 
      gggggaaaa agtccagga gtttttcac cgagaaaat 
      gaaaaacaa agttttcaa gttggccga ggtgctccg 
      ggggatcga cggcgacaa agccaagtt cagtctcaa 
      gtgcgacca ccccgagtg gttcgaagg ccttgacac 
      caatgcaaa aaccaaaga ggactatat gtttaggag 
      ggcgcagga ccggattcg gacctattt ctacaaaac 
      ccgagagga actgttgaa ggagcccac gctgccccc 
      aaaccggtt gcgaagcct cgtcgacga gcttcaaaa 
      ccgacttaa actgtccaa gttcaatgg acatttctt 
      cgatcgaag cgacaacaa gagcttgtg cgacgagag 
      aggcgagtt cacctgtca gggcaggtg gaacaaaga 
      catgtgtct ctacagccc ccaacatcg cattaatcc 
      ttacgaaag cagggaggg gaggattat cttccagac 
      gtggaattt ggaccacgt gattgaacg ctcgagggc 
      ggttatacc cgggatcgt gaaggcttt gaaagacgc 
      cgagtgcac gatcgttaa cgatcgcga ggcttcggt 
      cgatgtcaa agccatcta taacgattt gttcacttt 
      gcacaacct gaagtttgt acatattgt gtgtcatga 
      taagggtag gcatagcac gaaaaaggc agggccgta 
      tctgcttta ttaagccca cgtccttga taaatcgga 
      gacgaaccg cccactagt tttaagata ttcatttta 
      tgattaagc gacccaagg gcagaaaat ctgtagtta 
      acttagtct ttaaggctt ttcgtgtac aacttccaa 
      ctgccaaac tttttgttt tattacaaa agactgatt 
      ttgagaaaa atgagctta acccatcat cgggtctgt 
      cacagaacc attatattt ttaaatcaa tgtaacgaa 
      aaatgtagc tagagcttt acgttcgta tccacaaat 
      gcttatcaa gttataatg tgattatgc tatacaata 
      agacatata cacttattt atcatagta ttgtcaaag 
      tagtggctt gtagcttgt agacgcaat aatagtgta 
      gttggggat tgctggtac tgtgaaaaa ggctgtgtc 
      gggattgtt atccccttg ttagtgcta agtgactat 
      taatcatag attagccaa tcatatcat accggaaaa 
      atatgactg aaacatgtt gactgccat acaagtcat 
      accttgtag aacctatat attcaataa ataaatgtt 
      ttatctcaa aaaactttt attgctatt taatccgag 
      tacagataa ctcctactt tacaaacta agcaattga 
      ttcacacat gaatgatat aaattaaaa gaaatggaa 
      acaaatgaa atggctaca ccggactct cctacctaa 
      ataaatctt tggttgtta cgtcgatac attcgagac 
      ctgcaataa aataacaat aaacactac ctgttggcc 
      atcgatcgg acggaaaat cgggtcaag gatgtgccc 
      tacttttgg caggtagct ttttgggtc gaatccctg 
      gccgacctc atttcggtc tgacctgaa tcggccgga 
      ttgacctta gcacagcac tgacccgat tctgggggc 
      aaagggtca acgaggggc cgccaagga acgaacttg 
      attcacaaa aaatcatac taatacaga gcgttctaa 
      ata