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Gene information
General information
TC034036 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid54: 235328..353472
Sequence information
Transcripts / Proteins (4) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034036
    iBeetle screen iB_01030
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    tropharium partially degenerated - Number of animals showing the phenotype: 4

    egg chamber ectopically present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034036 RA
      MKYFSTGHK ESYTLIARK RHVKKLRQH QSNTTWHGT 
      TTALTGVQQ PPIRQSPDR LKGTTCDLF ELLEKAQSS 
      RLDDQRCVL PAYFNQAPL VQRQHSVPG PDPRDDCRL 
      STNNNVKIC TTPPSSPQQ ATQSRRLLQ ETLSRPPPY 
      PMIAVPEKG YWVDGTDHE VSFDHRGVP ILPHQTWRA 
      KIETDDTGK CYRRFYVGR EHTSLIGND ETLGPVLLS 
      IKTENVANQ EHTRILLRM RTGTMHEIV PSSCLGDSP 
      SPARMAKLL NEQLNIDNF VPVLHPKAS QLIANYDEH 
      VLVTNFKFG VMYQKFGQT TEEELFCNN ITSPAFDDF 
      LALLGQRIQ LKDHKGYRG GLDIQNGHT GDQAVYEVF 
      KDREIMFHV STLLPYTES DPQQLQRKR HIGNDIVAI 
      VFQEENTPF SPDMIASHF LHAFIVVQV VEPNTPNTR 
      YNVSVTARD DVPFFGPTL PTPAVFRHG PEFKEFLLT 
      KLINAENAC YKADKFAKL ELRTRTSLL QTLTEDLKE 
      KTNEFLGGS ATVVPGTPK SDSGPGSRF IDTVKKAWS 
      ARVKSSQSV DNNLANANG HERLSKKSS QPTISESTP 
      SSGRSLSKS SIVSNGKKS PNSSTASSP DLTAHAHAH 
      PAMSEASDD SSLTSEDLE EHLAGGYID SDTGLESMS 
      SAETAAKAC SVCQERPTS TTGPSSSEV LAQEVAKLK 
      CDKLDLLRQ NVSCQRDIK RLREKELSL QGDLEAAKK 
      EIIRLRELL KDYSPNGDR SPV
    • >TC034036 RB
      MRTLAETDR NTLPRGWVR QAVMGVLRW KDMPAAGRS 
      DTLHKGATL QAPLVQRQH SVPGPDPRD DCRLSTNNN 
      VKICTTPPS SPQQATQSR RLLQETLSR PPPYPMIAV 
      PEKGYWVDG TDHEVSFDH RGVPILPHQ TWRAKIETD 
      DTGKCYRRF YVGREHTSL IGNDETLGP VLLSIKTEN 
      VANQEHTRI LLRMRTGTM HEIVPSSCL GDSPSPARM 
      AKLLNEQLN IDNFVPVLH PKASQLIAN YDEHVLVTN 
      FKFGVMYQK FGQTTEEEL FCNNITSPA FDDFLALLG 
      QRIQLKDHK GYRGGLDIQ NGHTGDQAV YEVFKDREI 
      MFHVSTLLP YTESDPQQL QRKRHIGND IVAIVFQEE 
      NTPFSPDMI ASHFLHAFI VVQVVEPNT PNTRYNVSV 
      TARDDVPFF GPTLPTPAV FRHGPEFKE FLLTKLINA 
      ENACYKADK FAKLELRTR TSLLQTLTE DLKEKTNEF 
      LGGSATVVP GTPKSDSGP GSRFIDTVK KAWSARVKS 
      SQSVDNNLA NANGHERLS KKSSQPTIS ESTPSSGRS 
      LSKSSIVSN GKKSPNSST ASSPDLTAH AHAHPAMSE 
      ASDDSSLTS EDLEEHLAG GYIDSDTGL ESMSSAETA 
      AKACSVCQE RPTSTTGPS SSEVLAQEV AKLKCDKLD 
      LLRQNVSCQ RDIKRLREK ELSLQGDLE AAKKEIIRL 
      RELLKDYSP NGDRSPV
    • >TC034036 RC
      MLKIQMLEQ LVSNENTGR QHQSNTTWH GTTTALTGV 
      QQPPIRQSP DRLKGTTCD LFELLEKAQ SSRLDDQRC 
      VLPAYFNQV SVKTIKL
    • >TC034036 RD
      MKYFSTGHK ESYTLIARK RHVKKLRQH QSNTTWHGT 
      TTALTGVQQ PPIRQSPDR LKGTTCDLF ELLEKAQSS 
      RLDDQRCVL PAYFNQVSV KTIKL
    CDS sequence
    • >TC034036 RA
      atgaagtat ttcagcacg ggacacaag gagtcctac 
      actttgatc gcgcgcaag agacacgtg aagaaactg 
      cgccagcat cagagcaac acgacatgg cacggaacg 
      acaaccgct ctgacgggg gtgcaacag ccccccatc 
      aggcagagc ccggatagg ctcaaaggc acgacctgc 
      gacctattc gaactttta gaaaaagcc caaagttcc 
      agattagat gaccaaaga tgcgtatta ccagcctac 
      ttcaaccag gcgcccttg gtgcagaga cagcacagc 
      gttccaggg ccggatccc cgggacgat tgccgtctt 
      tcgaccaac aacaacgtg aaaatctgc accacgccg 
      ccgtcttcg ccccaacag gcaacgcag tcgcggagg 
      ctgctccag gagacgctc tcgcggccg cccccgtac 
      cccatgatc gccgtcccc gagaagggc tattgggtg 
      gatggcacc gaccatgag gtcagtttc gaccacaga 
      ggcgtgccc atcctcccg catcagacg tggagggcg 
      aagatcgaa accgacgat accggaaag tgctacagg 
      aggttctac gtcggaagg gaacacaca tccctgatc 
      ggcaacgac gagaccttg ggccctgtc ctcctgtcg 
      ataaaaacc gagaacgtg gccaaccag gagcacacc 
      cggatcctc ctccggatg cgcaccggg accatgcac 
      gagatcgtg ccctcctcc tgcctcggc gacagcccc 
      agcccggcc cgcatggcg aaactcctc aacgaacag 
      ctcaacatc gacaacttc gtcccggtg ttgcacccg 
      aaagcctcc caactcatc gccaactac gacgagcat 
      gtcctcgtt acgaacttc aaattcggc gtgatgtac 
      cagaagttc gggcagacg acggaagag gagctgttc 
      tgtaataac atcaccagt cccgccttt gacgatttc 
      cttgcgctg ctcgggcaa aggatacag ctcaaggat 
      cacaaggga tacaggggc ggcctggac atccagaac 
      gggcacacg ggggatcaa gcggtttac gaggtgttc 
      aaagaccgc gaaatcatg tttcatgtc tccacattg 
      ctgccctac acagaaagc gacccccaa cagttgcag 
      cggaagagg cacataggc aacgacatc gtggcgatc 
      gttttccaa gaggagaac acgcccttt tcgccagat 
      atgatagcg tcgcacttc ttgcacgcg tttatcgta 
      gtgcaggtg gtcgagccc aacacgccc aacacaagg 
      tacaacgtg agcgtgacg gcgcgtgac gacgtcccc 
      tttttcggt ccgacgctg ccgacgccc gccgtcttc 
      cgccacggc cccgaattc aaagagttc cttttgacg 
      aagttgatc aacgccgaa aacgcctgc tacaaagcc 
      gacaaattc gccaaattg gagctacgg acgcgcact 
      tcgctcctc cagactctc accgaagac ctcaaagag 
      aaaacgaac gaattcctg ggcggcagt gcgactgtc 
      gttcccgga acgccgaag agcgattcc gggcccggg 
      tcgcgcttc atcgacacc gtcaagaag gcgtggagt 
      gcgagggtt aagtcgagc caaagcgtc gataacaat 
      ttagctaat gcgaacgga cacgaacga ctgtcgaag 
      aagtcgagc cagccgacc atctccgag tcgacgcca 
      tcgagtggg cgttcgctg tcgaaaagt tcgatcgtt 
      tcgaacggg aagaagagt ccgaactcg tcgacggcg 
      tcctcgccc gatctcacg gcacacgct catgcacat 
      cctgcgatg tcggaggct agtgacgat tcgtccctc 
      accagtgag gacctggag gagcacttg gctgggggc 
      tatatcgac agtgacacc ggtctggag agcatgtcg 
      agtgccgaa acggcggcg aaggcgtgc agcgtgtgc 
      caggagagg cccacgagc acgacgggc cccagcagt 
      agcgaagtg ctcgcccag gaagtggca aaactcaag 
      tgtgataaa ctggacttg ttgagacag aatgtgagc 
      tgtcagcgg gacatcaag cggctgcga gagaaggag 
      ttgagtttg cagggcgac ctggaagcg gccaagaag 
      gaaatcatc aggttgcga gagctgctg aaggattac 
      agtcccaat ggggacaga tctccggtt taa
    • >TC034036 RB
      atgcgtacg ttagcggaa actgacagg aacacttta 
      ccgagaggg tgggttaga caggctgtg atgggtgtc 
      ttgaggtgg aaggatatg ccggcggcc ggcaggagc 
      gacactttg cacaaaggg gccaccctt caggcgccc 
      ttggtgcag agacagcac agcgttcca gggccggat 
      ccccgggac gattgccgt ctttcgacc aacaacaac 
      gtgaaaatc tgcaccacg ccgccgtct tcgccccaa 
      caggcaacg cagtcgcgg aggctgctc caggagacg 
      ctctcgcgg ccgcccccg taccccatg atcgccgtc 
      cccgagaag ggctattgg gtggatggc accgaccat 
      gaggtcagt ttcgaccac agaggcgtg cccatcctc 
      ccgcatcag acgtggagg gcgaagatc gaaaccgac 
      gataccgga aagtgctac aggaggttc tacgtcgga 
      agggaacac acatccctg atcggcaac gacgagacc 
      ttgggccct gtcctcctg tcgataaaa accgagaac 
      gtggccaac caggagcac acccggatc ctcctccgg 
      atgcgcacc gggaccatg cacgagatc gtgccctcc 
      tcctgcctc ggcgacagc cccagcccg gcccgcatg 
      gcgaaactc ctcaacgaa cagctcaac atcgacaac 
      ttcgtcccg gtgttgcac ccgaaagcc tcccaactc 
      atcgccaac tacgacgag catgtcctc gttacgaac 
      ttcaaattc ggcgtgatg taccagaag ttcgggcag 
      acgacggaa gaggagctg ttctgtaat aacatcacc 
      agtcccgcc tttgacgat ttccttgcg ctgctcggg 
      caaaggata cagctcaag gatcacaag ggatacagg 
      ggcggcctg gacatccag aacgggcac acgggggat 
      caagcggtt tacgaggtg ttcaaagac cgcgaaatc 
      atgtttcat gtctccaca ttgctgccc tacacagaa 
      agcgacccc caacagttg cagcggaag aggcacata 
      ggcaacgac atcgtggcg atcgttttc caagaggag 
      aacacgccc ttttcgcca gatatgata gcgtcgcac 
      ttcttgcac gcgtttatc gtagtgcag gtggtcgag 
      cccaacacg cccaacaca aggtacaac gtgagcgtg 
      acggcgcgt gacgacgtc ccctttttc ggtccgacg 
      ctgccgacg cccgccgtc ttccgccac ggccccgaa 
      ttcaaagag ttccttttg acgaagttg atcaacgcc 
      gaaaacgcc tgctacaaa gccgacaaa ttcgccaaa 
      ttggagcta cggacgcgc acttcgctc ctccagact 
      ctcaccgaa gacctcaaa gagaaaacg aacgaattc 
      ctgggcggc agtgcgact gtcgttccc ggaacgccg 
      aagagcgat tccgggccc gggtcgcgc ttcatcgac 
      accgtcaag aaggcgtgg agtgcgagg gttaagtcg 
      agccaaagc gtcgataac aatttagct aatgcgaac 
      ggacacgaa cgactgtcg aagaagtcg agccagccg 
      accatctcc gagtcgacg ccatcgagt gggcgttcg 
      ctgtcgaaa agttcgatc gtttcgaac gggaagaag 
      agtccgaac tcgtcgacg gcgtcctcg cccgatctc 
      acggcacac gctcatgca catcctgcg atgtcggag 
      gctagtgac gattcgtcc ctcaccagt gaggacctg 
      gaggagcac ttggctggg ggctatatc gacagtgac 
      accggtctg gagagcatg tcgagtgcc gaaacggcg 
      gcgaaggcg tgcagcgtg tgccaggag aggcccacg 
      agcacgacg ggccccagc agtagcgaa gtgctcgcc 
      caggaagtg gcaaaactc aagtgtgat aaactggac 
      ttgttgaga cagaatgtg agctgtcag cgggacatc 
      aagcggctg cgagagaag gagttgagt ttgcagggc 
      gacctggaa gcggccaag aaggaaatc atcaggttg 
      cgagagctg ctgaaggat tacagtccc aatggggac 
      agatctccg gtttaa
    • >TC034036 RC
      atgctgaag atacagatg ctggagcag ctcgtcagt 
      aacgaaaac actgggcgc cagcatcag agcaacacg 
      acatggcac ggaacgaca accgctctg acgggggtg 
      caacagccc cccatcagg cagagcccg gataggctc 
      aaaggcacg acctgcgac ctattcgaa cttttagaa 
      aaagcccaa agttccaga ttagatgac caaagatgc 
      gtattacca gcctacttc aaccaggtc agtgttaaa 
      actataaaa ctgtag
    • >TC034036 RD
      atgaagtat ttcagcacg ggacacaag gagtcctac 
      actttgatc gcgcgcaag agacacgtg aagaaactg 
      cgccagcat cagagcaac acgacatgg cacggaacg 
      acaaccgct ctgacgggg gtgcaacag ccccccatc 
      aggcagagc ccggatagg ctcaaaggc acgacctgc 
      gacctattc gaactttta gaaaaagcc caaagttcc 
      agattagat gaccaaaga tgcgtatta ccagcctac 
      ttcaaccag gtcagtgtt aaaactata aaactgtag
    mRNA sequence
    • >TC034036 RA
      tctctctat tcatccaaa cgagtcgct ttcagtagt 
      ctttcggac ggcatgcga agagaaagc tccgctcgc 
      agcggcttc agccgtcca cacacgtgt gcaatatcg 
      cgtcaagtg cagtgatgt aacggtcca ggtgcaata 
      gtgcagtgc aaaaatgaa gtatttcag cacgggaca 
      caaggagtc ctacacttt gatcgcgcg caagagaca 
      cgtgaagaa actgcgcca gcatcagag caacacgac 
      atggcacgg aacgacaac cgctctgac gggggtgca 
      acagccccc catcaggca gagcccgga taggctcaa 
      aggcacgac ctgcgacct attcgaact tttagaaaa 
      agcccaaag ttccagatt agatgacca aagatgcgt 
      attaccagc ctacttcaa ccaggcgcc cttggtgca 
      gagacagca cagcgttcc agggccgga tccccggga 
      cgattgccg tctttcgac caacaacaa cgtgaaaat 
      ctgcaccac gccgccgtc ttcgcccca acaggcaac 
      gcagtcgcg gaggctgct ccaggagac gctctcgcg 
      gccgccccc gtaccccat gatcgccgt ccccgagaa 
      gggctattg ggtggatgg caccgacca tgaggtcag 
      tttcgacca cagaggcgt gcccatcct cccgcatca 
      gacgtggag ggcgaagat cgaaaccga cgataccgg 
      aaagtgcta caggaggtt ctacgtcgg aagggaaca 
      cacatccct gatcggcaa cgacgagac cttgggccc 
      tgtcctcct gtcgataaa aaccgagaa cgtggccaa 
      ccaggagca cacccggat cctcctccg gatgcgcac 
      cgggaccat gcacgagat cgtgccctc ctcctgcct 
      cggcgacag ccccagccc ggcccgcat ggcgaaact 
      cctcaacga acagctcaa catcgacaa cttcgtccc 
      ggtgttgca cccgaaagc ctcccaact catcgccaa 
      ctacgacga gcatgtcct cgttacgaa cttcaaatt 
      cggcgtgat gtaccagaa gttcgggca gacgacgga 
      agaggagct gttctgtaa taacatcac cagtcccgc 
      ctttgacga tttccttgc gctgctcgg gcaaaggat 
      acagctcaa ggatcacaa gggatacag gggcggcct 
      ggacatcca gaacgggca cacggggga tcaagcggt 
      ttacgaggt gttcaaaga ccgcgaaat catgtttca 
      tgtctccac attgctgcc ctacacaga aagcgaccc 
      ccaacagtt gcagcggaa gaggcacat aggcaacga 
      catcgtggc gatcgtttt ccaagagga gaacacgcc 
      cttttcgcc agatatgat agcgtcgca cttcttgca 
      cgcgtttat cgtagtgca ggtggtcga gcccaacac 
      gcccaacac aaggtacaa cgtgagcgt gacggcgcg 
      tgacgacgt ccccttttt cggtccgac gctgccgac 
      gcccgccgt cttccgcca cggccccga attcaaaga 
      gttcctttt gacgaagtt gatcaacgc cgaaaacgc 
      ctgctacaa agccgacaa attcgccaa attggagct 
      acggacgcg cacttcgct cctccagac tctcaccga 
      agacctcaa agagaaaac gaacgaatt cctgggcgg 
      cagtgcgac tgtcgttcc cggaacgcc gaagagcga 
      ttccgggcc cgggtcgcg cttcatcga caccgtcaa 
      gaaggcgtg gagtgcgag ggttaagtc gagccaaag 
      cgtcgataa caatttagc taatgcgaa cggacacga 
      acgactgtc gaagaagtc gagccagcc gaccatctc 
      cgagtcgac gccatcgag tgggcgttc gctgtcgaa 
      aagttcgat cgtttcgaa cgggaagaa gagtccgaa 
      ctcgtcgac ggcgtcctc gcccgatct cacggcaca 
      cgctcatgc acatcctgc gatgtcgga ggctagtga 
      cgattcgtc cctcaccag tgaggacct ggaggagca 
      cttggctgg gggctatat cgacagtga caccggtct 
      ggagagcat gtcgagtgc cgaaacggc ggcgaaggc 
      gtgcagcgt gtgccagga gaggcccac gagcacgac 
      gggccccag cagtagcga agtgctcgc ccaggaagt 
      ggcaaaact caagtgtga taaactgga cttgttgag 
      acagaatgt gagctgtca gcgggacat caagcggct 
      gcgagagaa ggagttgag tttgcaggg cgacctgga 
      agcggccaa gaaggaaat catcaggtt gcgagagct 
      gctgaagga ttacagtcc caatgggga cagatctcc 
      ggtttaatt attgttatt attgcgttt cgttgcggt 
      aattattgt tttaatatt taaaataat cgattactt 
      tctgtttct tctcaaatc tttacatag ctgatatgt 
      aaacggtgt caattagat gtttgcaca ttacctaca 
      catagatgc atccacaca aaatacgta ttctcttca 
      ctgccttgt gatatcatc tgggttttt gtttcgcgt 
      tttcgtccc tttatgagt aaacagggg attttcgtt 
      ttttatttt agtcgcaat actcgaatg aacaacgaa 
      gttactcat tgagggagt gggcctttt ctttttctc 
      ttcgaagaa ttgtttagt tttttaaat atttattaa 
      tattgtctg tatagttat aatacaact ttaccaaca 
      aaatgatac caaagttaa cattcccca aaaattctg 
      tttcagaaa ttaaattat ttactaagt gtacataac 
      aaagtattt attagttcg taaggcgag cagagtttc 
      ctgaggata ttgttaata atgtagatt tctatattt 
      ttactctgt tcatttttt taagtttgt acaactgca 
      atatgtatt actaaagtt tagtaccca ttacattaa 
      gtttttatt ttgtacata ggaaacaca cgattacat 
      actcttcta actgagaac tgtaatagg tgtaatcaa 
      tgtctggca ttaactcct ccagacgaa tctatggcc 
      ttcttagga cgatctgaa gtgatacaa atattcatt 
      taagtgtaa tcgttacaa attacaatt atttaactc 
      tttattcga aagcttttt attttaagt acctattaa 
      ttcatacga ttattaagt gttctttgt atatagatt 
      atgtaactc ttttatgta ccttgattt gtcgtaacg 
      acgatgttt tctgcaaat aaattcatt aaatcaat
    • >TC034036 RB
      cgagctttc attggtcgg tttgttcgc tggaaaggt 
      ggcagcatg ctgtgtcct caaactcaa accgtttcg 
      aatgtttag ttcgcgact ggctgttgt tacgtcgat 
      gcgtgcttg tttttccta cactatgta gagtgagtg 
      gtgcgtttt tgaaattcc gtgccgcgc caaaaagtg 
      gtctagatg cgtacgtta gcggaaact gacaggaac 
      actttaccg agagggtgg gttagacag gctgtgatg 
      ggtgtcttg aggtggaag gatatgccg gcggccggc 
      aggagcgac actttgcac aaaggggcc acccttcag 
      gcgcccttg gtgcagaga cagcacagc gttccaggg 
      ccggatccc cgggacgat tgccgtctt tcgaccaac 
      aacaacgtg aaaatctgc accacgccg ccgtcttcg 
      ccccaacag gcaacgcag tcgcggagg ctgctccag 
      gagacgctc tcgcggccg cccccgtac cccatgatc 
      gccgtcccc gagaagggc tattgggtg gatggcacc 
      gaccatgag gtcagtttc gaccacaga ggcgtgccc 
      atcctcccg catcagacg tggagggcg aagatcgaa 
      accgacgat accggaaag tgctacagg aggttctac 
      gtcggaagg gaacacaca tccctgatc ggcaacgac 
      gagaccttg ggccctgtc ctcctgtcg ataaaaacc 
      gagaacgtg gccaaccag gagcacacc cggatcctc 
      ctccggatg cgcaccggg accatgcac gagatcgtg 
      ccctcctcc tgcctcggc gacagcccc agcccggcc 
      cgcatggcg aaactcctc aacgaacag ctcaacatc 
      gacaacttc gtcccggtg ttgcacccg aaagcctcc 
      caactcatc gccaactac gacgagcat gtcctcgtt 
      acgaacttc aaattcggc gtgatgtac cagaagttc 
      gggcagacg acggaagag gagctgttc tgtaataac 
      atcaccagt cccgccttt gacgatttc cttgcgctg 
      ctcgggcaa aggatacag ctcaaggat cacaaggga 
      tacaggggc ggcctggac atccagaac gggcacacg 
      ggggatcaa gcggtttac gaggtgttc aaagaccgc 
      gaaatcatg tttcatgtc tccacattg ctgccctac 
      acagaaagc gacccccaa cagttgcag cggaagagg 
      cacataggc aacgacatc gtggcgatc gttttccaa 
      gaggagaac acgcccttt tcgccagat atgatagcg 
      tcgcacttc ttgcacgcg tttatcgta gtgcaggtg 
      gtcgagccc aacacgccc aacacaagg tacaacgtg 
      agcgtgacg gcgcgtgac gacgtcccc tttttcggt 
      ccgacgctg ccgacgccc gccgtcttc cgccacggc 
      cccgaattc aaagagttc cttttgacg aagttgatc 
      aacgccgaa aacgcctgc tacaaagcc gacaaattc 
      gccaaattg gagctacgg acgcgcact tcgctcctc 
      cagactctc accgaagac ctcaaagag aaaacgaac 
      gaattcctg ggcggcagt gcgactgtc gttcccgga 
      acgccgaag agcgattcc gggcccggg tcgcgcttc 
      atcgacacc gtcaagaag gcgtggagt gcgagggtt 
      aagtcgagc caaagcgtc gataacaat ttagctaat 
      gcgaacgga cacgaacga ctgtcgaag aagtcgagc 
      cagccgacc atctccgag tcgacgcca tcgagtggg 
      cgttcgctg tcgaaaagt tcgatcgtt tcgaacggg 
      aagaagagt ccgaactcg tcgacggcg tcctcgccc 
      gatctcacg gcacacgct catgcacat cctgcgatg 
      tcggaggct agtgacgat tcgtccctc accagtgag 
      gacctggag gagcacttg gctgggggc tatatcgac 
      agtgacacc ggtctggag agcatgtcg agtgccgaa 
      acggcggcg aaggcgtgc agcgtgtgc caggagagg 
      cccacgagc acgacgggc cccagcagt agcgaagtg 
      ctcgcccag gaagtggca aaactcaag tgtgataaa 
      ctggacttg ttgagacag aatgtgagc tgtcagcgg 
      gacatcaag cggctgcga gagaaggag ttgagtttg 
      cagggcgac ctggaagcg gccaagaag gaaatcatc 
      aggttgcga gagctgctg aaggattac agtcccaat 
      ggggacaga tctccggtt taattattg ttattattg 
      cgtttcgtt gcggtaatt attgtttta atatttaaa 
      ataatcgat tactttctg tttcttctc aaatcttta 
      catagctga tatgtaaac ggtgtcaat tagatgttt 
      gcacattac ctacacata gatgcatcc acacaaaat 
      acgtattct cttcactgc cttgtgata tcatctggg 
      tttttgttt cgcgttttc gtcccttta tgagtaaac 
      aggggattt tcgtttttt attttagtc gcaatactc 
      gaatgaaca acgaagtta ctcattgag ggagtgggc 
      cttttcttt ttctcttcg aagaattgt ttagttttt 
      taaatattt attaatatt gtctgtata gttataata 
      caactttac caacaaaat gataccaaa gttaacatt 
      ccccaaaaa ttctgtttc agaaattaa attatttac 
      taagtgtac ataacaaag tatttatta gttcgtaag 
      gcgagcaga gtttcctga ggatattgt taataatgt 
      agatttcta tatttttac tctgttcat ttttttaag 
      tttgtacaa ctgcaatat gtattacta aagtttagt 
      acccattac attaagttt ttattttgt acataggaa 
      acacacgat tacatactc ttctaactg agaactgta 
      ataggtgta atcaatgtc tggcattaa ctcctccag 
      acgaatcta tggccttct taggacgat ctgaagtga 
      tacaaatat tcatttaag tgtaatcgt tacaaatta 
      caattattt aactcttta ttcgaaagc tttttattt 
      taagtacct attaattca tacgattat taagtgttc 
      tttgtatat agattatgt aactctttt atgtacctt 
      gatttgtcg taacgacga tgttttctg caaataaat 
      tcattaaat caat
    • >TC034036 RC
      gtcgaagag agagcgccg acgtcagga agatagttt 
      tatcatgat cgatggtct tggtgttgt agcaggagg 
      gaatcgcag gcggcagta gcacagttg ttggctgcg 
      gtgaattgt gggcggttt gttgtttgt aaagcgcga 
      gttttttta atttcgtga aagaaaacg cgtgctgga 
      agcttgttg ttgtgacga tgtgacgtg catcttcaa 
      aatgctgaa gatacagat gctggagca gctcgtcag 
      taacgaaaa cactgggcg ccagcatca gagcaacac 
      gacatggca cggaacgac aaccgctct gacgggggt 
      gcaacagcc ccccatcag gcagagccc ggataggct 
      caaaggcac gacctgcga cctattcga acttttaga 
      aaaagccca aagttccag attagatga ccaaagatg 
      cgtattacc agcctactt caaccaggt cagtgttaa 
      aactataaa actgtagtc actttatgt tttctatca 
      ctctaaacc aataaatga ataaaagat tt
    • >TC034036 RD
      tctctctat tcatccaaa cgagtcgct ttcagtagt 
      ctttcggac ggcatgcga agagaaagc tccgctcgc 
      agcggcttc agccgtcca cacacgtgt gcaatatcg 
      cgtcaagtg cagtgatgt aacggtcca ggtgcaata 
      gtgcagtgc aaaaatgaa gtatttcag cacgggaca 
      caaggagtc ctacacttt gatcgcgcg caagagaca 
      cgtgaagaa actgcgcca gcatcagag caacacgac 
      atggcacgg aacgacaac cgctctgac gggggtgca 
      acagccccc catcaggca gagcccgga taggctcaa 
      aggcacgac ctgcgacct attcgaact tttagaaaa 
      agcccaaag ttccagatt agatgacca aagatgcgt 
      attaccagc ctacttcaa ccaggtcag tgttaaaac 
      tataaaact gtagtcact ttatgtttt ctatcactc 
      taaaccaat aaatgaata aaagattt