| General information | |||
| TC034036 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 6854891..6973035 | |
| Sequence information | |||
| Transcripts / Proteins (4) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC034036 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01030 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Days post injection
Fertility
tropharium partially degenerated -
Number of animals showing the phenotype: 4
egg chamber ectopically present - Number of animals showing the phenotype: 4
egg chamber ectopically present - Number of animals showing the phenotype: 4
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 0.0% (includes death as larva, prepupa, pupa, adult)
- >TC034036 RA
MKYFSTGHK ESYTLIARK RHVKKLRQH QSNTTWHGT TTALTGVQQ PPIRQSPDR LKGTTCDLF ELLEKAQSS RLDDQRCVL PAYFNQAPL VQRQHSVPG PDPRDDCRL STNNNVKIC TTPPSSPQQ ATQSRRLLQ ETLSRPPPY PMIAVPEKG YWVDGTDHE VSFDHRGVP ILPHQTWRA KIETDDTGK CYRRFYVGR EHTSLIGND ETLGPVLLS IKTENVANQ EHTRILLRM RTGTMHEIV PSSCLGDSP SPARMAKLL NEQLNIDNF VPVLHPKAS QLIANYDEH VLVTNFKFG VMYQKFGQT TEEELFCNN ITSPAFDDF LALLGQRIQ LKDHKGYRG GLDIQNGHT GDQAVYEVF KDREIMFHV STLLPYTES DPQQLQRKR HIGNDIVAI VFQEENTPF SPDMIASHF LHAFIVVQV VEPNTPNTR YNVSVTARD DVPFFGPTL PTPAVFRHG PEFKEFLLT KLINAENAC YKADKFAKL ELRTRTSLL QTLTEDLKE KTNEFLGGS ATVVPGTPK SDSGPGSRF IDTVKKAWS ARVKSSQSV DNNLANANG HERLSKKSS QPTISESTP SSGRSLSKS SIVSNGKKS PNSSTASSP DLTAHAHAH PAMSEASDD SSLTSEDLE EHLAGGYID SDTGLESMS SAETAAKAC SVCQERPTS TTGPSSSEV LAQEVAKLK CDKLDLLRQ NVSCQRDIK RLREKELSL QGDLEAAKK EIIRLRELL KDYSPNGDR SPV
- >TC034036 RB
MRTLAETDR NTLPRGWVR QAVMGVLRW KDMPAAGRS DTLHKGATL QAPLVQRQH SVPGPDPRD DCRLSTNNN VKICTTPPS SPQQATQSR RLLQETLSR PPPYPMIAV PEKGYWVDG TDHEVSFDH RGVPILPHQ TWRAKIETD DTGKCYRRF YVGREHTSL IGNDETLGP VLLSIKTEN VANQEHTRI LLRMRTGTM HEIVPSSCL GDSPSPARM AKLLNEQLN IDNFVPVLH PKASQLIAN YDEHVLVTN FKFGVMYQK FGQTTEEEL FCNNITSPA FDDFLALLG QRIQLKDHK GYRGGLDIQ NGHTGDQAV YEVFKDREI MFHVSTLLP YTESDPQQL QRKRHIGND IVAIVFQEE NTPFSPDMI ASHFLHAFI VVQVVEPNT PNTRYNVSV TARDDVPFF GPTLPTPAV FRHGPEFKE FLLTKLINA ENACYKADK FAKLELRTR TSLLQTLTE DLKEKTNEF LGGSATVVP GTPKSDSGP GSRFIDTVK KAWSARVKS SQSVDNNLA NANGHERLS KKSSQPTIS ESTPSSGRS LSKSSIVSN GKKSPNSST ASSPDLTAH AHAHPAMSE ASDDSSLTS EDLEEHLAG GYIDSDTGL ESMSSAETA AKACSVCQE RPTSTTGPS SSEVLAQEV AKLKCDKLD LLRQNVSCQ RDIKRLREK ELSLQGDLE AAKKEIIRL RELLKDYSP NGDRSPV
- >TC034036 RC
MLKIQMLEQ LVSNENTGR QHQSNTTWH GTTTALTGV QQPPIRQSP DRLKGTTCD LFELLEKAQ SSRLDDQRC VLPAYFNQV SVKTIKL
- >TC034036 RD
MKYFSTGHK ESYTLIARK RHVKKLRQH QSNTTWHGT TTALTGVQQ PPIRQSPDR LKGTTCDLF ELLEKAQSS RLDDQRCVL PAYFNQVSV KTIKL
- >TC034036 RA
atgaagtat ttcagcacg ggacacaag gagtcctac actttgatc gcgcgcaag agacacgtg aagaaactg cgccagcat cagagcaac acgacatgg cacggaacg acaaccgct ctgacgggg gtgcaacag ccccccatc aggcagagc ccggatagg ctcaaaggc acgacctgc gacctattc gaactttta gaaaaagcc caaagttcc agattagat gaccaaaga tgcgtatta ccagcctac ttcaaccag gcgcccttg gtgcagaga cagcacagc gttccaggg ccggatccc cgggacgat tgccgtctt tcgaccaac aacaacgtg aaaatctgc accacgccg ccgtcttcg ccccaacag gcaacgcag tcgcggagg ctgctccag gagacgctc tcgcggccg cccccgtac cccatgatc gccgtcccc gagaagggc tattgggtg gatggcacc gaccatgag gtcagtttc gaccacaga ggcgtgccc atcctcccg catcagacg tggagggcg aagatcgaa accgacgat accggaaag tgctacagg aggttctac gtcggaagg gaacacaca tccctgatc ggcaacgac gagaccttg ggccctgtc ctcctgtcg ataaaaacc gagaacgtg gccaaccag gagcacacc cggatcctc ctccggatg cgcaccggg accatgcac gagatcgtg ccctcctcc tgcctcggc gacagcccc agcccggcc cgcatggcg aaactcctc aacgaacag ctcaacatc gacaacttc gtcccggtg ttgcacccg aaagcctcc caactcatc gccaactac gacgagcat gtcctcgtt acgaacttc aaattcggc gtgatgtac cagaagttc gggcagacg acggaagag gagctgttc tgtaataac atcaccagt cccgccttt gacgatttc cttgcgctg ctcgggcaa aggatacag ctcaaggat cacaaggga tacaggggc ggcctggac atccagaac gggcacacg ggggatcaa gcggtttac gaggtgttc aaagaccgc gaaatcatg tttcatgtc tccacattg ctgccctac acagaaagc gacccccaa cagttgcag cggaagagg cacataggc aacgacatc gtggcgatc gttttccaa gaggagaac acgcccttt tcgccagat atgatagcg tcgcacttc ttgcacgcg tttatcgta gtgcaggtg gtcgagccc aacacgccc aacacaagg tacaacgtg agcgtgacg gcgcgtgac gacgtcccc tttttcggt ccgacgctg ccgacgccc gccgtcttc cgccacggc cccgaattc aaagagttc cttttgacg aagttgatc aacgccgaa aacgcctgc tacaaagcc gacaaattc gccaaattg gagctacgg acgcgcact tcgctcctc cagactctc accgaagac ctcaaagag aaaacgaac gaattcctg ggcggcagt gcgactgtc gttcccgga acgccgaag agcgattcc gggcccggg tcgcgcttc atcgacacc gtcaagaag gcgtggagt gcgagggtt aagtcgagc caaagcgtc gataacaat ttagctaat gcgaacgga cacgaacga ctgtcgaag aagtcgagc cagccgacc atctccgag tcgacgcca tcgagtggg cgttcgctg tcgaaaagt tcgatcgtt tcgaacggg aagaagagt ccgaactcg tcgacggcg tcctcgccc gatctcacg gcacacgct catgcacat cctgcgatg tcggaggct agtgacgat tcgtccctc accagtgag gacctggag gagcacttg gctgggggc tatatcgac agtgacacc ggtctggag agcatgtcg agtgccgaa acggcggcg aaggcgtgc agcgtgtgc caggagagg cccacgagc acgacgggc cccagcagt agcgaagtg ctcgcccag gaagtggca aaactcaag tgtgataaa ctggacttg ttgagacag aatgtgagc tgtcagcgg gacatcaag cggctgcga gagaaggag ttgagtttg cagggcgac ctggaagcg gccaagaag gaaatcatc aggttgcga gagctgctg aaggattac agtcccaat ggggacaga tctccggtt taa
- >TC034036 RB
atgcgtacg ttagcggaa actgacagg aacacttta ccgagaggg tgggttaga caggctgtg atgggtgtc ttgaggtgg aaggatatg ccggcggcc ggcaggagc gacactttg cacaaaggg gccaccctt caggcgccc ttggtgcag agacagcac agcgttcca gggccggat ccccgggac gattgccgt ctttcgacc aacaacaac gtgaaaatc tgcaccacg ccgccgtct tcgccccaa caggcaacg cagtcgcgg aggctgctc caggagacg ctctcgcgg ccgcccccg taccccatg atcgccgtc cccgagaag ggctattgg gtggatggc accgaccat gaggtcagt ttcgaccac agaggcgtg cccatcctc ccgcatcag acgtggagg gcgaagatc gaaaccgac gataccgga aagtgctac aggaggttc tacgtcgga agggaacac acatccctg atcggcaac gacgagacc ttgggccct gtcctcctg tcgataaaa accgagaac gtggccaac caggagcac acccggatc ctcctccgg atgcgcacc gggaccatg cacgagatc gtgccctcc tcctgcctc ggcgacagc cccagcccg gcccgcatg gcgaaactc ctcaacgaa cagctcaac atcgacaac ttcgtcccg gtgttgcac ccgaaagcc tcccaactc atcgccaac tacgacgag catgtcctc gttacgaac ttcaaattc ggcgtgatg taccagaag ttcgggcag acgacggaa gaggagctg ttctgtaat aacatcacc agtcccgcc tttgacgat ttccttgcg ctgctcggg caaaggata cagctcaag gatcacaag ggatacagg ggcggcctg gacatccag aacgggcac acgggggat caagcggtt tacgaggtg ttcaaagac cgcgaaatc atgtttcat gtctccaca ttgctgccc tacacagaa agcgacccc caacagttg cagcggaag aggcacata ggcaacgac atcgtggcg atcgttttc caagaggag aacacgccc ttttcgcca gatatgata gcgtcgcac ttcttgcac gcgtttatc gtagtgcag gtggtcgag cccaacacg cccaacaca aggtacaac gtgagcgtg acggcgcgt gacgacgtc ccctttttc ggtccgacg ctgccgacg cccgccgtc ttccgccac ggccccgaa ttcaaagag ttccttttg acgaagttg atcaacgcc gaaaacgcc tgctacaaa gccgacaaa ttcgccaaa ttggagcta cggacgcgc acttcgctc ctccagact ctcaccgaa gacctcaaa gagaaaacg aacgaattc ctgggcggc agtgcgact gtcgttccc ggaacgccg aagagcgat tccgggccc gggtcgcgc ttcatcgac accgtcaag aaggcgtgg agtgcgagg gttaagtcg agccaaagc gtcgataac aatttagct aatgcgaac ggacacgaa cgactgtcg aagaagtcg agccagccg accatctcc gagtcgacg ccatcgagt gggcgttcg ctgtcgaaa agttcgatc gtttcgaac gggaagaag agtccgaac tcgtcgacg gcgtcctcg cccgatctc acggcacac gctcatgca catcctgcg atgtcggag gctagtgac gattcgtcc ctcaccagt gaggacctg gaggagcac ttggctggg ggctatatc gacagtgac accggtctg gagagcatg tcgagtgcc gaaacggcg gcgaaggcg tgcagcgtg tgccaggag aggcccacg agcacgacg ggccccagc agtagcgaa gtgctcgcc caggaagtg gcaaaactc aagtgtgat aaactggac ttgttgaga cagaatgtg agctgtcag cgggacatc aagcggctg cgagagaag gagttgagt ttgcagggc gacctggaa gcggccaag aaggaaatc atcaggttg cgagagctg ctgaaggat tacagtccc aatggggac agatctccg gtttaa
- >TC034036 RC
atgctgaag atacagatg ctggagcag ctcgtcagt aacgaaaac actgggcgc cagcatcag agcaacacg acatggcac ggaacgaca accgctctg acgggggtg caacagccc cccatcagg cagagcccg gataggctc aaaggcacg acctgcgac ctattcgaa cttttagaa aaagcccaa agttccaga ttagatgac caaagatgc gtattacca gcctacttc aaccaggtc agtgttaaa actataaaa ctgtag
- >TC034036 RD
atgaagtat ttcagcacg ggacacaag gagtcctac actttgatc gcgcgcaag agacacgtg aagaaactg cgccagcat cagagcaac acgacatgg cacggaacg acaaccgct ctgacgggg gtgcaacag ccccccatc aggcagagc ccggatagg ctcaaaggc acgacctgc gacctattc gaactttta gaaaaagcc caaagttcc agattagat gaccaaaga tgcgtatta ccagcctac ttcaaccag gtcagtgtt aaaactata aaactgtag
- >TC034036 RA
tctctctat tcatccaaa cgagtcgct ttcagtagt ctttcggac ggcatgcga agagaaagc tccgctcgc agcggcttc agccgtcca cacacgtgt gcaatatcg cgtcaagtg cagtgatgt aacggtcca ggtgcaata gtgcagtgc aaaaatgaa gtatttcag cacgggaca caaggagtc ctacacttt gatcgcgcg caagagaca cgtgaagaa actgcgcca gcatcagag caacacgac atggcacgg aacgacaac cgctctgac gggggtgca acagccccc catcaggca gagcccgga taggctcaa aggcacgac ctgcgacct attcgaact tttagaaaa agcccaaag ttccagatt agatgacca aagatgcgt attaccagc ctacttcaa ccaggcgcc cttggtgca gagacagca cagcgttcc agggccgga tccccggga cgattgccg tctttcgac caacaacaa cgtgaaaat ctgcaccac gccgccgtc ttcgcccca acaggcaac gcagtcgcg gaggctgct ccaggagac gctctcgcg gccgccccc gtaccccat gatcgccgt ccccgagaa gggctattg ggtggatgg caccgacca tgaggtcag tttcgacca cagaggcgt gcccatcct cccgcatca gacgtggag ggcgaagat cgaaaccga cgataccgg aaagtgcta caggaggtt ctacgtcgg aagggaaca cacatccct gatcggcaa cgacgagac cttgggccc tgtcctcct gtcgataaa aaccgagaa cgtggccaa ccaggagca cacccggat cctcctccg gatgcgcac cgggaccat gcacgagat cgtgccctc ctcctgcct cggcgacag ccccagccc ggcccgcat ggcgaaact cctcaacga acagctcaa catcgacaa cttcgtccc ggtgttgca cccgaaagc ctcccaact catcgccaa ctacgacga gcatgtcct cgttacgaa cttcaaatt cggcgtgat gtaccagaa gttcgggca gacgacgga agaggagct gttctgtaa taacatcac cagtcccgc ctttgacga tttccttgc gctgctcgg gcaaaggat acagctcaa ggatcacaa gggatacag gggcggcct ggacatcca gaacgggca cacggggga tcaagcggt ttacgaggt gttcaaaga ccgcgaaat catgtttca tgtctccac attgctgcc ctacacaga aagcgaccc ccaacagtt gcagcggaa gaggcacat aggcaacga catcgtggc gatcgtttt ccaagagga gaacacgcc cttttcgcc agatatgat agcgtcgca cttcttgca cgcgtttat cgtagtgca ggtggtcga gcccaacac gcccaacac aaggtacaa cgtgagcgt gacggcgcg tgacgacgt ccccttttt cggtccgac gctgccgac gcccgccgt cttccgcca cggccccga attcaaaga gttcctttt gacgaagtt gatcaacgc cgaaaacgc ctgctacaa agccgacaa attcgccaa attggagct acggacgcg cacttcgct cctccagac tctcaccga agacctcaa agagaaaac gaacgaatt cctgggcgg cagtgcgac tgtcgttcc cggaacgcc gaagagcga ttccgggcc cgggtcgcg cttcatcga caccgtcaa gaaggcgtg gagtgcgag ggttaagtc gagccaaag cgtcgataa caatttagc taatgcgaa cggacacga acgactgtc gaagaagtc gagccagcc gaccatctc cgagtcgac gccatcgag tgggcgttc gctgtcgaa aagttcgat cgtttcgaa cgggaagaa gagtccgaa ctcgtcgac ggcgtcctc gcccgatct cacggcaca cgctcatgc acatcctgc gatgtcgga ggctagtga cgattcgtc cctcaccag tgaggacct ggaggagca cttggctgg gggctatat cgacagtga caccggtct ggagagcat gtcgagtgc cgaaacggc ggcgaaggc gtgcagcgt gtgccagga gaggcccac gagcacgac gggccccag cagtagcga agtgctcgc ccaggaagt ggcaaaact caagtgtga taaactgga cttgttgag acagaatgt gagctgtca gcgggacat caagcggct gcgagagaa ggagttgag tttgcaggg cgacctgga agcggccaa gaaggaaat catcaggtt gcgagagct gctgaagga ttacagtcc caatgggga cagatctcc ggtttaatt attgttatt attgcgttt cgttgcggt aattattgt tttaatatt taaaataat cgattactt tctgtttct tctcaaatc tttacatag ctgatatgt aaacggtgt caattagat gtttgcaca ttacctaca catagatgc atccacaca aaatacgta ttctcttca ctgccttgt gatatcatc tgggttttt gtttcgcgt tttcgtccc tttatgagt aaacagggg attttcgtt ttttatttt agtcgcaat actcgaatg aacaacgaa gttactcat tgagggagt gggcctttt ctttttctc ttcgaagaa ttgtttagt tttttaaat atttattaa tattgtctg tatagttat aatacaact ttaccaaca aaatgatac caaagttaa cattcccca aaaattctg tttcagaaa ttaaattat ttactaagt gtacataac aaagtattt attagttcg taaggcgag cagagtttc ctgaggata ttgttaata atgtagatt tctatattt ttactctgt tcatttttt taagtttgt acaactgca atatgtatt actaaagtt tagtaccca ttacattaa gtttttatt ttgtacata ggaaacaca cgattacat actcttcta actgagaac tgtaatagg tgtaatcaa tgtctggca ttaactcct ccagacgaa tctatggcc ttcttagga cgatctgaa gtgatacaa atattcatt taagtgtaa tcgttacaa attacaatt atttaactc tttattcga aagcttttt attttaagt acctattaa ttcatacga ttattaagt gttctttgt atatagatt atgtaactc ttttatgta ccttgattt gtcgtaacg acgatgttt tctgcaaat aaattcatt aaatcaat
- >TC034036 RB
cgagctttc attggtcgg tttgttcgc tggaaaggt ggcagcatg ctgtgtcct caaactcaa accgtttcg aatgtttag ttcgcgact ggctgttgt tacgtcgat gcgtgcttg tttttccta cactatgta gagtgagtg gtgcgtttt tgaaattcc gtgccgcgc caaaaagtg gtctagatg cgtacgtta gcggaaact gacaggaac actttaccg agagggtgg gttagacag gctgtgatg ggtgtcttg aggtggaag gatatgccg gcggccggc aggagcgac actttgcac aaaggggcc acccttcag gcgcccttg gtgcagaga cagcacagc gttccaggg ccggatccc cgggacgat tgccgtctt tcgaccaac aacaacgtg aaaatctgc accacgccg ccgtcttcg ccccaacag gcaacgcag tcgcggagg ctgctccag gagacgctc tcgcggccg cccccgtac cccatgatc gccgtcccc gagaagggc tattgggtg gatggcacc gaccatgag gtcagtttc gaccacaga ggcgtgccc atcctcccg catcagacg tggagggcg aagatcgaa accgacgat accggaaag tgctacagg aggttctac gtcggaagg gaacacaca tccctgatc ggcaacgac gagaccttg ggccctgtc ctcctgtcg ataaaaacc gagaacgtg gccaaccag gagcacacc cggatcctc ctccggatg cgcaccggg accatgcac gagatcgtg ccctcctcc tgcctcggc gacagcccc agcccggcc cgcatggcg aaactcctc aacgaacag ctcaacatc gacaacttc gtcccggtg ttgcacccg aaagcctcc caactcatc gccaactac gacgagcat gtcctcgtt acgaacttc aaattcggc gtgatgtac cagaagttc gggcagacg acggaagag gagctgttc tgtaataac atcaccagt cccgccttt gacgatttc cttgcgctg ctcgggcaa aggatacag ctcaaggat cacaaggga tacaggggc ggcctggac atccagaac gggcacacg ggggatcaa gcggtttac gaggtgttc aaagaccgc gaaatcatg tttcatgtc tccacattg ctgccctac acagaaagc gacccccaa cagttgcag cggaagagg cacataggc aacgacatc gtggcgatc gttttccaa gaggagaac acgcccttt tcgccagat atgatagcg tcgcacttc ttgcacgcg tttatcgta gtgcaggtg gtcgagccc aacacgccc aacacaagg tacaacgtg agcgtgacg gcgcgtgac gacgtcccc tttttcggt ccgacgctg ccgacgccc gccgtcttc cgccacggc cccgaattc aaagagttc cttttgacg aagttgatc aacgccgaa aacgcctgc tacaaagcc gacaaattc gccaaattg gagctacgg acgcgcact tcgctcctc cagactctc accgaagac ctcaaagag aaaacgaac gaattcctg ggcggcagt gcgactgtc gttcccgga acgccgaag agcgattcc gggcccggg tcgcgcttc atcgacacc gtcaagaag gcgtggagt gcgagggtt aagtcgagc caaagcgtc gataacaat ttagctaat gcgaacgga cacgaacga ctgtcgaag aagtcgagc cagccgacc atctccgag tcgacgcca tcgagtggg cgttcgctg tcgaaaagt tcgatcgtt tcgaacggg aagaagagt ccgaactcg tcgacggcg tcctcgccc gatctcacg gcacacgct catgcacat cctgcgatg tcggaggct agtgacgat tcgtccctc accagtgag gacctggag gagcacttg gctgggggc tatatcgac agtgacacc ggtctggag agcatgtcg agtgccgaa acggcggcg aaggcgtgc agcgtgtgc caggagagg cccacgagc acgacgggc cccagcagt agcgaagtg ctcgcccag gaagtggca aaactcaag tgtgataaa ctggacttg ttgagacag aatgtgagc tgtcagcgg gacatcaag cggctgcga gagaaggag ttgagtttg cagggcgac ctggaagcg gccaagaag gaaatcatc aggttgcga gagctgctg aaggattac agtcccaat ggggacaga tctccggtt taattattg ttattattg cgtttcgtt gcggtaatt attgtttta atatttaaa ataatcgat tactttctg tttcttctc aaatcttta catagctga tatgtaaac ggtgtcaat tagatgttt gcacattac ctacacata gatgcatcc acacaaaat acgtattct cttcactgc cttgtgata tcatctggg tttttgttt cgcgttttc gtcccttta tgagtaaac aggggattt tcgtttttt attttagtc gcaatactc gaatgaaca acgaagtta ctcattgag ggagtgggc cttttcttt ttctcttcg aagaattgt ttagttttt taaatattt attaatatt gtctgtata gttataata caactttac caacaaaat gataccaaa gttaacatt ccccaaaaa ttctgtttc agaaattaa attatttac taagtgtac ataacaaag tatttatta gttcgtaag gcgagcaga gtttcctga ggatattgt taataatgt agatttcta tatttttac tctgttcat ttttttaag tttgtacaa ctgcaatat gtattacta aagtttagt acccattac attaagttt ttattttgt acataggaa acacacgat tacatactc ttctaactg agaactgta ataggtgta atcaatgtc tggcattaa ctcctccag acgaatcta tggccttct taggacgat ctgaagtga tacaaatat tcatttaag tgtaatcgt tacaaatta caattattt aactcttta ttcgaaagc tttttattt taagtacct attaattca tacgattat taagtgttc tttgtatat agattatgt aactctttt atgtacctt gatttgtcg taacgacga tgttttctg caaataaat tcattaaat caat
- >TC034036 RC
gtcgaagag agagcgccg acgtcagga agatagttt tatcatgat cgatggtct tggtgttgt agcaggagg gaatcgcag gcggcagta gcacagttg ttggctgcg gtgaattgt gggcggttt gttgtttgt aaagcgcga gttttttta atttcgtga aagaaaacg cgtgctgga agcttgttg ttgtgacga tgtgacgtg catcttcaa aatgctgaa gatacagat gctggagca gctcgtcag taacgaaaa cactgggcg ccagcatca gagcaacac gacatggca cggaacgac aaccgctct gacgggggt gcaacagcc ccccatcag gcagagccc ggataggct caaaggcac gacctgcga cctattcga acttttaga aaaagccca aagttccag attagatga ccaaagatg cgtattacc agcctactt caaccaggt cagtgttaa aactataaa actgtagtc actttatgt tttctatca ctctaaacc aataaatga ataaaagat tt
- >TC034036 RD
tctctctat tcatccaaa cgagtcgct ttcagtagt ctttcggac ggcatgcga agagaaagc tccgctcgc agcggcttc agccgtcca cacacgtgt gcaatatcg cgtcaagtg cagtgatgt aacggtcca ggtgcaata gtgcagtgc aaaaatgaa gtatttcag cacgggaca caaggagtc ctacacttt gatcgcgcg caagagaca cgtgaagaa actgcgcca gcatcagag caacacgac atggcacgg aacgacaac cgctctgac gggggtgca acagccccc catcaggca gagcccgga taggctcaa aggcacgac ctgcgacct attcgaact tttagaaaa agcccaaag ttccagatt agatgacca aagatgcgt attaccagc ctacttcaa ccaggtcag tgttaaaac tataaaact gtagtcact ttatgtttt ctatcactc taaaccaat aaatgaata aaagattt
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