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Gene information
General information
TC006303 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6812742..6814371
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006303
    iBeetle screen iB_01029

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006303 RA
      MKLSVTLVL FLCHNIWSY DYETKYFEV LLDHFSFTN 
NATFKLKYL INDTFWTND GPIFFYTGN EGTVENFAE 
NTGFMFDIA PSFNALVVF AEHRYYGES LPFGNDSFV 
SPSHIGYLT SSQALADFV DLINYLQTM SLEKVPVIA 
FGGSYGGML ASWLRMKYP ASVVGAIAA SAPIWQFET 
PCEDFYKVV TRVYQEAVA KDCPLLITK SWTALRNIS 
ESPEGKAWL SDAWQLCSP LETSADVET LIGWYSEIL 
VNMAMVNYP YSTSFLAPL PPFPVKTFC SQLTQANIV 
DDKSLVMAL GDALQIYTN FTETTTCNK INQTAEALG 
EEGWYFQAC TEMIMPMCS IDGDMFEND PWDYGKYAS 
QCFEKWGVN QTHPELPVL EYGGKEIKA ASNIVFSNG 
LLDPWSSGG VLKNVSESV VSVIIPDGA HHIDLRGGN 
KDDPETVIE ARQFHVDNI KKWIMEFYF HSGKGAFFE 
KIKYQHVAR N
    CDS sequence
    • >TC006303 RA
      atgaaacta agcgtaacg ttggtgtta ttcttgtgt 
cacaatatt tggtcttac gattatgaa acgaaatat 
ttcgaagta cttttagat cacttcagt ttcaccaat 
aatgccact tttaaatta aaatattta atcaatgat 
actttttgg acaaacgat ggcccgatt tttttctac 
acagggaat gaagggact gttgaaaat ttcgccgaa 
aataccgga tttatgttc gatattgcg cccagtttt 
aatgctttg gtagttttc gccgaacat cggtactat 
ggcgaaagt ttgcctttt ggtaacgat tctttcgtg 
tcaccgagt catataggg taccttact tcaagtcaa 
gcccttgct gactttgtc gacttgatc aattacctc 
caaacaatg agccttgaa aaagtacca gtcattgct 
tttggtggc tcttacggt ggaatgcta gcctcatgg 
cttagaatg aagtatccg gcatcagtc gttggtgca 
attgcagct tcagcccca atttggcaa tttgagacc 
ccgtgtgag gatttttac aaagttgtt acgagagtg 
taccaagaa gctgtggct aaagattgc cctcttttg 
atcactaaa tcatggact gcgttaaga aatatctcg 
gaaagtcca gaaggaaaa gcctggctt tcggacgct 
tggcagttg tgttcccct cttgaaaca tcagcagat 
gtcgaaacc ctaatcggg tggtattcc gagattttg 
gtcaacatg gccatggtt aactacccc tactctacg 
tcatttctg gcacctttg cccccattt ccggttaaa 
accttctgt tcgcaactt acgcaagca aacatcgtc 
gatgataag tccctagtt atggccctc ggagacgcg 
ttgcaaatt tacacgaat tttaccgaa accacaact 
tgtaataaa atcaatcaa acggctgaa gcactcgga 
gaggagggc tggtacttc caggcttgt accgagatg 
attatgccg atgtgttcc atcgatggg gatatgttc 
gaaaatgat ccatgggat tatggaaaa tatgcgagt 
cagtgtttt gaaaaatgg ggagtgaat caaacacat 
ccagaattg ccggtgctt gaatatgga gggaaggag 
atcaaagcg gccagtaat attgttttt agtaacggt 
ttactcgat ccttggtcg agtggcggt gttttgaag 
aatgtgtcc gaaagtgtt gtttccgtg attatccca 
gatggggcc catcatatt gatttgcga ggagggaac 
aaagatgat cccgaaacg gtgattgaa gcgaggcag 
tttcatgtc gataatatt aaaaaatgg atcatggag 
ttctatttt catagtggg aaaggggct tttttcgag 
aagattaag taccaacat gttgccaga aattaa
    mRNA sequence
    • >TC006303 RA
      ataaatgac ttaagtctc gactaacat aatttttcg 
atagttacc acaatgaaa ctaagcgta acgttggtg 
ttattcttg tgtcacaat atttggtct tacgattat 
gaaacgaaa tatttcgaa gtactttta gatcacttc 
agtttcacc aataatgcc acttttaaa ttaaaatat 
ttaatcaat gatactttt tggacaaac gatggcccg 
atttttttc tacacaggg aatgaaggg actgttgaa 
aatttcgcc gaaaatacc ggatttatg ttcgatatt 
gcgcccagt tttaatgct ttggtagtt ttcgccgaa 
catcggtac tatggcgaa agtttgcct tttggtaac 
gattctttc gtgtcaccg agtcatata gggtacctt 
acttcaagt caagccctt gctgacttt gtcgacttg 
atcaattac ctccaaaca atgagcctt gaaaaagta 
ccagtcatt gcttttggt ggctcttac ggtggaatg 
ctagcctca tggcttaga atgaagtat ccggcatca 
gtcgttggt gcaattgca gcttcagcc ccaatttgg 
caatttgag accccgtgt gaggatttt tacaaagtt 
gttacgaga gtgtaccaa gaagctgtg gctaaagat 
tgccctctt ttgatcact aaatcatgg actgcgtta 
agaaatatc tcggaaagt ccagaagga aaagcctgg 
ctttcggac gcttggcag ttgtgttcc cctcttgaa 
acatcagca gatgtcgaa accctaatc gggtggtat 
tccgagatt ttggtcaac atggccatg gttaactac 
ccctactct acgtcattt ctggcacct ttgccccca 
tttccggtt aaaaccttc tgttcgcaa cttacgcaa 
gcaaacatc gtcgatgat aagtcccta gttatggcc 
ctcggagac gcgttgcaa atttacacg aattttacc 
gaaaccaca acttgtaat aaaatcaat caaacggct 
gaagcactc ggagaggag ggctggtac ttccaggct 
tgtaccgag atgattatg ccgatgtgt tccatcgat 
ggggatatg ttcgaaaat gatccatgg gattatgga 
aaatatgcg agtcagtgt tttgaaaaa tggggagtg 
aatcaaaca catccagaa ttgccggtg cttgaatat 
ggagggaag gagatcaaa gcggccagt aatattgtt 
tttagtaac ggtttactc gatccttgg tcgagtggc 
ggtgttttg aagaatgtg tccgaaagt gttgtttcc 
gtgattatc ccagatggg gcccatcat attgatttg 
cgaggaggg aacaaagat gatcccgaa acggtgatt 
gaagcgagg cagtttcat gtcgataat attaaaaaa 
tggatcatg gagttctat tttcatagt gggaaaggg 
gcttttttc gagaagatt aagtaccaa catgttgcc 
agaaattaa attagtttg tatgagaac aaagtagtg 
cctaaataa aaaatagaa atgata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY