Status
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Gene information
General information
TC006301 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6762779..6780010
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006301
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01028

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection



    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006301 RA
      MIKSNKCHL SAMSPAELV KHGEHEQEW GGYFVVKGH 
      ERLVRMLLM TRRNYPIAI KRSGWKARG SIFSDCGIS 
      MRCVREDQT ATTNVLHFV TDGTAKLMF SYKKVLYYT 
      PLVLILKCL CDYCDQFIY QKLIQGYQH DSYYLDCVQ 
      NMLRNLHIE NLHTHEDCK VYLGKMFRV KFYECPDWL 
      TDSEIADFI LKKCIMIHL DKPEDKFNM LVFMCQKLF 
      VFVQDKCKP EGADAVMMQ ELLLGGHLY LQLIKERLQ 
      GWLFGLKSN ILKRSSTLT GHLSQTEIL QAAKRSGGL 
      ENAFEMFLA TGNITSSSG LGLMQDKGL TIVAENINR 
      MRYMSHFKA VHRGAFFQE MRTTEVRQL LPDAWGFIC 
      PVHTPDGAP CGLLNHLTT NCSVTDVPD ARKVENIPI 
      ILTRLGMVA LKYVASVGF DQNYVVVLD GRVLGYVRR 
      DLAPKLVEQ LRLCKIKGE EVPNTLEIV LVPLMKVTA 
      QFPGLYLFT GPARMMRPL LNLKTKEVE FVGTFEQVY 
      MDICVSPDE YYKGVTTHM ELSKLAFLS NLAQLIPMP 
      DCNQSPRNM YQCQMGKQT MGTPCHNWR SQSETKLYR 
      LQTPATPLF RPVHYDNIN LDDFAMGTN AIVAVISYT 
      GYDMEDAMI INKAADERG FAHGSIYKT EFVTLEHES 
      SYFCRNPEK PELAEFLDT DGLPIIGRR MKDGDPLYC 
      YYSVDESVY KVTKFSGKE ECFVDMVKL CASFETTNR 
      TACFTFRVP RNPNVGDKF ASRAGQKGI CSQKWPMED 
      LPFTETGLV PDIVFNPHG FPSRMTIAM MIEVMAGKS 
      AALHGLVHD ATPFKFTEE DTAIDYFGR LLEAGGYNY 
      YGTETMYSG IDGREMTAQ IFFGIVHYQ RLRHMVSDK 
      WQVRSTGPT DILTRQPLK GRKRGGGVR FGEMERDSL 
      ISHGASFLL QDRLFHCSD KTTAFICTS CGTLLGPVT 
      VITRVAEKA NQSTCKETC RLCGHGRDI SEIQIPYIF 
      KFFVTQMAS CNINVKMLT QHV
    CDS sequence
    • >TC006301 RA
      atgattaaa tcgaataag tgccacttg agcgccatg 
      tcccccgca gagttggtc aaacacggg gagcatgag 
      caggaatgg gggggctat tttgtggtc aaaggccac 
      gagagacta gtaaggatg ttgctaatg acacgtcgg 
      aactatcca attgctata aaacgctcg ggctggaaa 
      gcgcggggc tcgattttt tcagactgt ggtatttcc 
      atgaggtgt gtcagggag gaccaaacg gccactacg 
      aatgtttta cattttgtt acggatgga accgccaaa 
      ttaatgttc agttacaag aaagttttg tactacacg 
      ccgttagtc ctgattttg aaatgtttg tgcgattat 
      tgcgaccag tttatttac cagaagttg atacaaggg 
      taccagcac gattcctac tacttggat tgcgtccaa 
      aacatgtta cgcaacctc cacatcgaa aacctccac 
      acgcacgaa gactgcaaa gtctattta ggcaaaatg 
      ttccgcgtg aaattttac gaatgtccc gactggtta 
      accgactcc gaaatcgcc gacttcatc ctcaaaaaa 
      tgcataatg atccacctc gacaaaccc gaagacaaa 
      ttcaacatg ttggtcttc atgtgccaa aaactgttc 
      gttttcgtg caagacaag tgcaaacct gagggtgcc 
      gacgccgtc atgatgcaa gagttgctc ctagggggc 
      cacctctac ttgcaactg atcaaagag cggctccaa 
      ggctggtta ttcggcctc aaatcgaac attttgaaa 
      cgcagcagc accttaacc ggccacttg tcccaaacc 
      gagattcta caagcggcc aaacggtca ggtggcttg 
      gaaaacgcc ttcgagatg ttcctggcc actgggaac 
      atcaccagt tcctcaggc ttgggttta atgcaagac 
      aagggcttg acaatagtt gcggaaaac atcaatcgg 
      atgcgctac atgtcgcac ttcaaggcc gtccatcgg 
      ggagccttc ttccaggag atgcgcacc acggaggtg 
      cgccagctg ctcccggac gcttggggc ttcatctgc 
      cccgtgcac acccccgat ggggccccc tgcggcctg 
      ctgaaccac ctgaccacg aattgctcc gttactgat 
      gttcccgat gcgcggaaa gttgaaaat attcccatt 
      attttgacg cgcttggga atggtggct ttgaaatat 
      gtggcaagt gttgggttt gatcagaat tatgtggtg 
      gtgcttgat ggcagggtt ttggggtat gtgaggagg 
      gatttggcc ccaaagttg gtcgaacag ttgcggttg 
      tgtaagatt aaaggggag gaagtgccg aatacgctt 
      gaaattgtt ttggttccg ttgatgaaa gttacagca 
      cagtttccg gggctgtat ttgtttacg gggccggcg 
      cgtatgatg aggcctttg ttgaatttg aagacgaag 
      gaagtggag tttgtgggg actttcgag caggtttat 
      atggatatt tgcgtctcg ccggatgag tattacaaa 
      ggtgttacg acccacatg gagctctcc aaactggcc 
      ttcctctca aacctagcc caactaatc cccatgccc 
      gactgcaac caatcaccc cgtaacatg taccagtgc 
      cagatgggc aaacaaaca atgggcact ccttgccac 
      aactggcgt tcccaatcc gaaaccaaa ctctaccgc 
      ctccaaaca ccggcgact ccccttttc cgacccgtc 
      cactacgac aacatcaac cttgacgac ttcgccatg 
      ggcacaaac gccattgtt gccgttatc agctacact 
      ggctatgac atggaagac gcaatgata ataaacaaa 
      gccgcggac gagcgtggt tttgcccac ggttccatt 
      tacaaaacc gaatttgta acacttgaa cacgagtct 
      tcgtatttt tgccgaaac ccggaaaaa ccggaactg 
      gccgaattc ttagacacg gacggcttg ccaatcatc 
      ggacgccga atgaaagat ggggatccc ctgtactgt 
      tactacagt gtggacgag tcggtgtac aaagtaacc 
      aagttttcg gggaaagag gagtgtttt gtcgacatg 
      gttaaactg tgcgccagt tttgaaacg accaatcgg 
      acggcgtgt ttcacgttc agagtcccg cgtaacccg 
      aacgttggc gataaattc gcaagtcgg gcaggacaa 
      aagggcatt tgttcgcaa aagtggccc atggaggac 
      ttacctttt acggagaca gggcttgtg cctgatatt 
      gtcttcaac cctcatggg tttccctct cgtatgacc 
      atcgctatg atgattgag gtcatggct gggaaatcg 
      gcagcgctt catgggcta gtacatgat gcaactccc 
      tttaaattt acagaagaa gacactgcg attgattat 
      tttggtcgg ttgttggaa gcagggggt tataattat 
      tacgggact gagaccatg tattcgggg attgatggt 
      agggaaatg acagcgcaa atatttttc gggatcgtt 
      cactaccag cgcttgaga cacatggtt tcggataag 
      tggcaggtg cgttcaact gggccgacc gatattttg 
      acgagacag cccctcaaa gggcggaaa cgaggaggg 
      ggtgtgcga tttggagag atggaacgt gattcgttg 
      atcagtcat ggcgcttcg tttttgtta caagatcgc 
      ttattccac tgttccgat aaaacaaca gcatttatc 
      tgcacctct tgtggcact ctattgggt ccagtgact 
      gtgataacc cgagtggct gaaaaagcg aaccaatcc 
      acttgcaag gaaacttgt aggttgtgt ggccacggc 
      cgagacatt tccgagata caaatccct tacattttc 
      aagtttttt gtaacccaa atggcgtcg tgtaacata 
      aatgtaaaa atgttaaca caacacgtg taa
    mRNA sequence
    • >TC006301 RA
      ggccagtgc ccatcatga ttaaatcga ataagtgcc 
      acttgagcg ccatgtccc ccgcagagt tggtcaaac 
      acggggagc atgagcagg aatgggggg gctattttg 
      tggtcaaag gccacgaga gactagtaa ggatgttgc 
      taatgacac gtcggaact atccaattg ctataaaac 
      gctcgggct ggaaagcgc ggggctcga ttttttcag 
      actgtggta tttccatga ggtgtgtca gggaggacc 
      aaacggcca ctacgaatg ttttacatt ttgttacgg 
      atggaaccg ccaaattaa tgttcagtt acaagaaag 
      ttttgtact acacgccgt tagtcctga ttttgaaat 
      gtttgtgcg attattgcg accagttta tttaccaga 
      agttgatac aagggtacc agcacgatt cctactact 
      tggattgcg tccaaaaca tgttacgca acctccaca 
      tcgaaaacc tccacacgc acgaagact gcaaagtct 
      atttaggca aaatgttcc gcgtgaaat tttacgaat 
      gtcccgact ggttaaccg actccgaaa tcgccgact 
      tcatcctca aaaaatgca taatgatcc acctcgaca 
      aacccgaag acaaattca acatgttgg tcttcatgt 
      gccaaaaac tgttcgttt tcgtgcaag acaagtgca 
      aacctgagg gtgccgacg ccgtcatga tgcaagagt 
      tgctcctag ggggccacc tctacttgc aactgatca 
      aagagcggc tccaaggct ggttattcg gcctcaaat 
      cgaacattt tgaaacgca gcagcacct taaccggcc 
      acttgtccc aaaccgaga ttctacaag cggccaaac 
      ggtcaggtg gcttggaaa acgccttcg agatgttcc 
      tggccactg ggaacatca ccagttcct caggcttgg 
      gtttaatgc aagacaagg gcttgacaa tagttgcgg 
      aaaacatca atcggatgc gctacatgt cgcacttca 
      aggccgtcc atcggggag ccttcttcc aggagatgc 
      gcaccacgg aggtgcgcc agctgctcc cggacgctt 
      ggggcttca tctgccccg tgcacaccc ccgatgggg 
      ccccctgcg gcctgctga accacctga ccacgaatt 
      gctccgtta ctgatgttc ccgatgcgc ggaaagttg 
      aaaatattc ccattattt tgacgcgct tgggaatgg 
      tggctttga aatatgtgg caagtgttg ggtttgatc 
      agaattatg tggtggtgc ttgatggca gggttttgg 
      ggtatgtga ggagggatt tggccccaa agttggtcg 
      aacagttgc ggttgtgta agattaaag gggaggaag 
      tgccgaata cgcttgaaa ttgttttgg ttccgttga 
      tgaaagtta cagcacagt ttccggggc tgtatttgt 
      ttacggggc cggcgcgta tgatgaggc ctttgttga 
      atttgaaga cgaaggaag tggagtttg tggggactt 
      tcgagcagg tttatatgg atatttgcg tctcgccgg 
      atgagtatt acaaaggtg ttacgaccc acatggagc 
      tctccaaac tggccttcc tctcaaacc tagcccaac 
      taatcccca tgcccgact gcaaccaat caccccgta 
      acatgtacc agtgccaga tgggcaaac aaacaatgg 
      gcactcctt gccacaact ggcgttccc aatccgaaa 
      ccaaactct accgcctcc aaacaccgg cgactcccc 
      ttttccgac ccgtccact acgacaaca tcaaccttg 
      acgacttcg ccatgggca caaacgcca ttgttgccg 
      ttatcagct acactggct atgacatgg aagacgcaa 
      tgataataa acaaagccg cggacgagc gtggttttg 
      cccacggtt ccatttaca aaaccgaat ttgtaacac 
      ttgaacacg agtcttcgt atttttgcc gaaacccgg 
      aaaaaccgg aactggccg aattcttag acacggacg 
      gcttgccaa tcatcggac gccgaatga aagatgggg 
      atcccctgt actgttact acagtgtgg acgagtcgg 
      tgtacaaag taaccaagt tttcgggga aagaggagt 
      gttttgtcg acatggtta aactgtgcg ccagttttg 
      aaacgacca atcggacgg cgtgtttca cgttcagag 
      tcccgcgta acccgaacg ttggcgata aattcgcaa 
      gtcgggcag gacaaaagg gcatttgtt cgcaaaagt 
      ggcccatgg aggacttac cttttacgg agacagggc 
      ttgtgcctg atattgtct tcaaccctc atgggtttc 
      cctctcgta tgaccatcg ctatgatga ttgaggtca 
      tggctggga aatcggcag cgcttcatg ggctagtac 
      atgatgcaa ctcccttta aatttacag aagaagaca 
      ctgcgattg attattttg gtcggttgt tggaagcag 
      ggggttata attattacg ggactgaga ccatgtatt 
      cggggattg atggtaggg aaatgacag cgcaaatat 
      ttttcggga tcgttcact accagcgct tgagacaca 
      tggtttcgg ataagtggc aggtgcgtt caactgggc 
      cgaccgata ttttgacga gacagcccc tcaaagggc 
      ggaaacgag gagggggtg tgcgatttg gagagatgg 
      aacgtgatt cgttgatca gtcatggcg cttcgtttt 
      tgttacaag atcgcttat tccactgtt ccgataaaa 
      caacagcat ttatctgca cctcttgtg gcactctat 
      tgggtccag tgactgtga taacccgag tggctgaaa 
      aagcgaacc aatccactt gcaaggaaa cttgtaggt 
      tgtgtggcc acggccgag acatttccg agatacaaa 
      tcccttaca ttttcaagt tttttgtaa cccaaatgg 
      cgtcgtgta acataaatg taaaaatgt taacacaac 
      acgtgtaat ttttttttt ttgtaataa aagagaaaa 
      taaacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY