Status
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Gene information
General information
TC006301 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6762779..6780010
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006301
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01028

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 3 (16 dpi)
    Days post injection

    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 4 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 3 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006301 RA
      MIKSNKCHL SAMSPAELV KHGEHEQEW GGYFVVKGH 
ERLVRMLLM TRRNYPIAI KRSGWKARG SIFSDCGIS 
MRCVREDQT ATTNVLHFV TDGTAKLMF SYKKVLYYT 
PLVLILKCL CDYCDQFIY QKLIQGYQH DSYYLDCVQ 
NMLRNLHIE NLHTHEDCK VYLGKMFRV KFYECPDWL 
TDSEIADFI LKKCIMIHL DKPEDKFNM LVFMCQKLF 
VFVQDKCKP EGADAVMMQ ELLLGGHLY LQLIKERLQ 
GWLFGLKSN ILKRSSTLT GHLSQTEIL QAAKRSGGL 
ENAFEMFLA TGNITSSSG LGLMQDKGL TIVAENINR 
MRYMSHFKA VHRGAFFQE MRTTEVRQL LPDAWGFIC 
PVHTPDGAP CGLLNHLTT NCSVTDVPD ARKVENIPI 
ILTRLGMVA LKYVASVGF DQNYVVVLD GRVLGYVRR 
DLAPKLVEQ LRLCKIKGE EVPNTLEIV LVPLMKVTA 
QFPGLYLFT GPARMMRPL LNLKTKEVE FVGTFEQVY 
MDICVSPDE YYKGVTTHM ELSKLAFLS NLAQLIPMP 
DCNQSPRNM YQCQMGKQT MGTPCHNWR SQSETKLYR 
LQTPATPLF RPVHYDNIN LDDFAMGTN AIVAVISYT 
GYDMEDAMI INKAADERG FAHGSIYKT EFVTLEHES 
SYFCRNPEK PELAEFLDT DGLPIIGRR MKDGDPLYC 
YYSVDESVY KVTKFSGKE ECFVDMVKL CASFETTNR 
TACFTFRVP RNPNVGDKF ASRAGQKGI CSQKWPMED 
LPFTETGLV PDIVFNPHG FPSRMTIAM MIEVMAGKS 
AALHGLVHD ATPFKFTEE DTAIDYFGR LLEAGGYNY 
YGTETMYSG IDGREMTAQ IFFGIVHYQ RLRHMVSDK 
WQVRSTGPT DILTRQPLK GRKRGGGVR FGEMERDSL 
ISHGASFLL QDRLFHCSD KTTAFICTS CGTLLGPVT 
VITRVAEKA NQSTCKETC RLCGHGRDI SEIQIPYIF 
KFFVTQMAS CNINVKMLT QHV
    CDS sequence
    • >TC006301 RA
      atgattaaa tcgaataag tgccacttg agcgccatg 
tcccccgca gagttggtc aaacacggg gagcatgag 
caggaatgg gggggctat tttgtggtc aaaggccac 
gagagacta gtaaggatg ttgctaatg acacgtcgg 
aactatcca attgctata aaacgctcg ggctggaaa 
gcgcggggc tcgattttt tcagactgt ggtatttcc 
atgaggtgt gtcagggag gaccaaacg gccactacg 
aatgtttta cattttgtt acggatgga accgccaaa 
ttaatgttc agttacaag aaagttttg tactacacg 
ccgttagtc ctgattttg aaatgtttg tgcgattat 
tgcgaccag tttatttac cagaagttg atacaaggg 
taccagcac gattcctac tacttggat tgcgtccaa 
aacatgtta cgcaacctc cacatcgaa aacctccac 
acgcacgaa gactgcaaa gtctattta ggcaaaatg 
ttccgcgtg aaattttac gaatgtccc gactggtta 
accgactcc gaaatcgcc gacttcatc ctcaaaaaa 
tgcataatg atccacctc gacaaaccc gaagacaaa 
ttcaacatg ttggtcttc atgtgccaa aaactgttc 
gttttcgtg caagacaag tgcaaacct gagggtgcc 
gacgccgtc atgatgcaa gagttgctc ctagggggc 
cacctctac ttgcaactg atcaaagag cggctccaa 
ggctggtta ttcggcctc aaatcgaac attttgaaa 
cgcagcagc accttaacc ggccacttg tcccaaacc 
gagattcta caagcggcc aaacggtca ggtggcttg 
gaaaacgcc ttcgagatg ttcctggcc actgggaac 
atcaccagt tcctcaggc ttgggttta atgcaagac 
aagggcttg acaatagtt gcggaaaac atcaatcgg 
atgcgctac atgtcgcac ttcaaggcc gtccatcgg 
ggagccttc ttccaggag atgcgcacc acggaggtg 
cgccagctg ctcccggac gcttggggc ttcatctgc 
cccgtgcac acccccgat ggggccccc tgcggcctg 
ctgaaccac ctgaccacg aattgctcc gttactgat 
gttcccgat gcgcggaaa gttgaaaat attcccatt 
attttgacg cgcttggga atggtggct ttgaaatat 
gtggcaagt gttgggttt gatcagaat tatgtggtg 
gtgcttgat ggcagggtt ttggggtat gtgaggagg 
gatttggcc ccaaagttg gtcgaacag ttgcggttg 
tgtaagatt aaaggggag gaagtgccg aatacgctt 
gaaattgtt ttggttccg ttgatgaaa gttacagca 
cagtttccg gggctgtat ttgtttacg gggccggcg 
cgtatgatg aggcctttg ttgaatttg aagacgaag 
gaagtggag tttgtgggg actttcgag caggtttat 
atggatatt tgcgtctcg ccggatgag tattacaaa 
ggtgttacg acccacatg gagctctcc aaactggcc 
ttcctctca aacctagcc caactaatc cccatgccc 
gactgcaac caatcaccc cgtaacatg taccagtgc 
cagatgggc aaacaaaca atgggcact ccttgccac 
aactggcgt tcccaatcc gaaaccaaa ctctaccgc 
ctccaaaca ccggcgact ccccttttc cgacccgtc 
cactacgac aacatcaac cttgacgac ttcgccatg 
ggcacaaac gccattgtt gccgttatc agctacact 
ggctatgac atggaagac gcaatgata ataaacaaa 
gccgcggac gagcgtggt tttgcccac ggttccatt 
tacaaaacc gaatttgta acacttgaa cacgagtct 
tcgtatttt tgccgaaac ccggaaaaa ccggaactg 
gccgaattc ttagacacg gacggcttg ccaatcatc 
ggacgccga atgaaagat ggggatccc ctgtactgt 
tactacagt gtggacgag tcggtgtac aaagtaacc 
aagttttcg gggaaagag gagtgtttt gtcgacatg 
gttaaactg tgcgccagt tttgaaacg accaatcgg 
acggcgtgt ttcacgttc agagtcccg cgtaacccg 
aacgttggc gataaattc gcaagtcgg gcaggacaa 
aagggcatt tgttcgcaa aagtggccc atggaggac 
ttacctttt acggagaca gggcttgtg cctgatatt 
gtcttcaac cctcatggg tttccctct cgtatgacc 
atcgctatg atgattgag gtcatggct gggaaatcg 
gcagcgctt catgggcta gtacatgat gcaactccc 
tttaaattt acagaagaa gacactgcg attgattat 
tttggtcgg ttgttggaa gcagggggt tataattat 
tacgggact gagaccatg tattcgggg attgatggt 
agggaaatg acagcgcaa atatttttc gggatcgtt 
cactaccag cgcttgaga cacatggtt tcggataag 
tggcaggtg cgttcaact gggccgacc gatattttg 
acgagacag cccctcaaa gggcggaaa cgaggaggg 
ggtgtgcga tttggagag atggaacgt gattcgttg 
atcagtcat ggcgcttcg tttttgtta caagatcgc 
ttattccac tgttccgat aaaacaaca gcatttatc 
tgcacctct tgtggcact ctattgggt ccagtgact 
gtgataacc cgagtggct gaaaaagcg aaccaatcc 
acttgcaag gaaacttgt aggttgtgt ggccacggc 
cgagacatt tccgagata caaatccct tacattttc 
aagtttttt gtaacccaa atggcgtcg tgtaacata 
aatgtaaaa atgttaaca caacacgtg taa
    mRNA sequence
    • >TC006301 RA
      ggccagtgc ccatcatga ttaaatcga ataagtgcc 
acttgagcg ccatgtccc ccgcagagt tggtcaaac 
acggggagc atgagcagg aatgggggg gctattttg 
tggtcaaag gccacgaga gactagtaa ggatgttgc 
taatgacac gtcggaact atccaattg ctataaaac 
gctcgggct ggaaagcgc ggggctcga ttttttcag 
actgtggta tttccatga ggtgtgtca gggaggacc 
aaacggcca ctacgaatg ttttacatt ttgttacgg 
atggaaccg ccaaattaa tgttcagtt acaagaaag 
ttttgtact acacgccgt tagtcctga ttttgaaat 
gtttgtgcg attattgcg accagttta tttaccaga 
agttgatac aagggtacc agcacgatt cctactact 
tggattgcg tccaaaaca tgttacgca acctccaca 
tcgaaaacc tccacacgc acgaagact gcaaagtct 
atttaggca aaatgttcc gcgtgaaat tttacgaat 
gtcccgact ggttaaccg actccgaaa tcgccgact 
tcatcctca aaaaatgca taatgatcc acctcgaca 
aacccgaag acaaattca acatgttgg tcttcatgt 
gccaaaaac tgttcgttt tcgtgcaag acaagtgca 
aacctgagg gtgccgacg ccgtcatga tgcaagagt 
tgctcctag ggggccacc tctacttgc aactgatca 
aagagcggc tccaaggct ggttattcg gcctcaaat 
cgaacattt tgaaacgca gcagcacct taaccggcc 
acttgtccc aaaccgaga ttctacaag cggccaaac 
ggtcaggtg gcttggaaa acgccttcg agatgttcc 
tggccactg ggaacatca ccagttcct caggcttgg 
gtttaatgc aagacaagg gcttgacaa tagttgcgg 
aaaacatca atcggatgc gctacatgt cgcacttca 
aggccgtcc atcggggag ccttcttcc aggagatgc 
gcaccacgg aggtgcgcc agctgctcc cggacgctt 
ggggcttca tctgccccg tgcacaccc ccgatgggg 
ccccctgcg gcctgctga accacctga ccacgaatt 
gctccgtta ctgatgttc ccgatgcgc ggaaagttg 
aaaatattc ccattattt tgacgcgct tgggaatgg 
tggctttga aatatgtgg caagtgttg ggtttgatc 
agaattatg tggtggtgc ttgatggca gggttttgg 
ggtatgtga ggagggatt tggccccaa agttggtcg 
aacagttgc ggttgtgta agattaaag gggaggaag 
tgccgaata cgcttgaaa ttgttttgg ttccgttga 
tgaaagtta cagcacagt ttccggggc tgtatttgt 
ttacggggc cggcgcgta tgatgaggc ctttgttga 
atttgaaga cgaaggaag tggagtttg tggggactt 
tcgagcagg tttatatgg atatttgcg tctcgccgg 
atgagtatt acaaaggtg ttacgaccc acatggagc 
tctccaaac tggccttcc tctcaaacc tagcccaac 
taatcccca tgcccgact gcaaccaat caccccgta 
acatgtacc agtgccaga tgggcaaac aaacaatgg 
gcactcctt gccacaact ggcgttccc aatccgaaa 
ccaaactct accgcctcc aaacaccgg cgactcccc 
ttttccgac ccgtccact acgacaaca tcaaccttg 
acgacttcg ccatgggca caaacgcca ttgttgccg 
ttatcagct acactggct atgacatgg aagacgcaa 
tgataataa acaaagccg cggacgagc gtggttttg 
cccacggtt ccatttaca aaaccgaat ttgtaacac 
ttgaacacg agtcttcgt atttttgcc gaaacccgg 
aaaaaccgg aactggccg aattcttag acacggacg 
gcttgccaa tcatcggac gccgaatga aagatgggg 
atcccctgt actgttact acagtgtgg acgagtcgg 
tgtacaaag taaccaagt tttcgggga aagaggagt 
gttttgtcg acatggtta aactgtgcg ccagttttg 
aaacgacca atcggacgg cgtgtttca cgttcagag 
tcccgcgta acccgaacg ttggcgata aattcgcaa 
gtcgggcag gacaaaagg gcatttgtt cgcaaaagt 
ggcccatgg aggacttac cttttacgg agacagggc 
ttgtgcctg atattgtct tcaaccctc atgggtttc 
cctctcgta tgaccatcg ctatgatga ttgaggtca 
tggctggga aatcggcag cgcttcatg ggctagtac 
atgatgcaa ctcccttta aatttacag aagaagaca 
ctgcgattg attattttg gtcggttgt tggaagcag 
ggggttata attattacg ggactgaga ccatgtatt 
cggggattg atggtaggg aaatgacag cgcaaatat 
ttttcggga tcgttcact accagcgct tgagacaca 
tggtttcgg ataagtggc aggtgcgtt caactgggc 
cgaccgata ttttgacga gacagcccc tcaaagggc 
ggaaacgag gagggggtg tgcgatttg gagagatgg 
aacgtgatt cgttgatca gtcatggcg cttcgtttt 
tgttacaag atcgcttat tccactgtt ccgataaaa 
caacagcat ttatctgca cctcttgtg gcactctat 
tgggtccag tgactgtga taacccgag tggctgaaa 
aagcgaacc aatccactt gcaaggaaa cttgtaggt 
tgtgtggcc acggccgag acatttccg agatacaaa 
tcccttaca ttttcaagt tttttgtaa cccaaatgg 
cgtcgtgta acataaatg taaaaatgt taacacaac 
acgtgtaat ttttttttt ttgtaataa aagagaaaa 
taaacg

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY