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Gene information
General information
TC034028
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006291
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6584169..6585981
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034028
    iBeetle screen iB_01027

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late not present - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 62.5% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    pupal molt delayed (still pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Adult morphology
    elytra partially pigmentation/tanning decreased - Number of animals showing the phenotype: 3


    Fertility
    egg production: number no/almost no eggs -
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034028 RA
      MLKPLALLL VLAGSQCAP LGDLYLPDD DVEILTIEN 
      FKRYVENST SAWLVEFYA SWCGYCQRF APPWKQFAT 
      EAAPWRDLV RVAVLECSD EINTPICRD FGIVKYPTV 
      RYFHENSHF DGGDKGVIV PREFPVTVD AIKKNVIER 
      FMTEMGEGR GVVYPNLLP YLHSDLEPF FDEEDDDIF 
      YGFLVVEDS DSYLGGEVA LDLHKTPNV TIRHALNNN 
      TKLVKNLQI GKFPTLVII DRNNNTQIV TENIEHKKE 
      LKATIADYL AKKGLKVCE TTPEKKGHL SLDPHPDPK 
      QRSRTLLRQ KIKKMGDAV FQMDLETSL RYALLREVS 
      TTKVIKGEQ LAALRAFLN VIKKYFPFG YNSTSFINN 
      LTNLTSSDE VQGVQVQVL VQQADDSGV FSTPQRFLG 
      CQGSANRFR GYPCSLWRL FHYLTVNSV LLNVSNRKA 
      NPVEVLGAM HGYVKHFFS CSHCSEHFQ KMAAERNLT 
      SVSSLEESV LWLWEAHNV VNKRLKGDT TEDPEYPKE 
      QFPTRLRCP ECYGEDGTW RKKEVLKYL KRMYGRYSV 
      RYVGSDTKV LFPGLDR
    CDS sequence
    • >TC034028 RA
      atgctcaaa ccactagct ctcctcctc gtgctagcc 
      ggcagccag tgcgccccc ctaggcgac ctctacctc 
      cccgacgac gacgtcgaa atcctcaca atcgagaac 
      ttcaaacgc tacgtcgaa aactccaca agcgcctgg 
      ctggtggag ttctacgcc agctggtgc ggctactgc 
      caacgcttc gcgccccct tggaagcag ttcgccacg 
      gaggcggcc ccctggcgg gacctggtg cgggtggcg 
      gtgttggag tgctccgac gagatcaac acgccgatt 
      tgtcgcgac tttggaatt gtcaaatac ccaacagtt 
      cggtatttc cacgaaaat tcgcacttt gatgggggc 
      gacaagggg gttattgtg ccccgggag ttcccagtg 
      accgtggac gcgatcaaa aagaacgta atcgagaga 
      ttcatgacc gaaatgggc gaaggccga ggggtggtt 
      taccccaat ttgttaccc tacctccat tcggacctg 
      gagccgttt ttcgacgaa gaagacgac gatattttt 
      tacggtttt ttggtggtt gaggattct gatagttat 
      ttggggggc gaagtagcg ctagacctt cacaaaact 
      ccaaacgtt acgatcaga cacgccctt aataataac 
      acaaagttg gtgaaaaat ttacaaatt gggaaattc 
      ccaacactt gttataatc gatcggaac aacaacacg 
      caaattgtt accgaaaat atcgaacac aagaaggag 
      ctcaaagcg acaattgcc gattacttg gccaagaaa 
      gggctcaaa gtttgcgag accacccct gagaaaaag 
      ggccacttg tccctggac ccccaccct gaccccaaa 
      cagcgctca cgcacactt ttgcggcaa aaaatcaaa 
      aaaatggga gacgccgtc ttccaaatg gacttggaa 
      acgtccctt cggtacgcc cttttgcgc gaagtctcg 
      accactaaa gtgatcaaa ggcgaacaa ctggcggcc 
      cttagggcc ttcctcaac gtgatcaag aagtatttc 
      cccttcggg tacaactcc acgtctttc atcaacaat 
      ttaaccaac ttgacttct tcggacgaa gtccagggg 
      gtgcaggtc caggtcctg gtgcagcaa gctgatgac 
      agtggggtg ttttcaacc ccgcagcgc ttcctgggg 
      tgtcagggg agtgcgaac cgctttcgc ggatatcca 
      tgtagtctt tggcgcctt ttccattac ctaacagtt 
      aactcggtt cttttgaat gtgagtaac cggaaggcg 
      aatccggtg gaagtcctc ggagcgatg cacggctac 
      gtcaagcac ttcttcagc tgtagtcac tgcagtgag 
      catttccag aagatggcc gctgagagg aatctaacg 
      agtgtttcg tcgcttgaa gagtcggtt ttgtggctc 
      tgggaggcg cataatgtg gtcaataaa agattaaaa 
      ggtgacaca accgaagat ccggagtat ccgaaagaa 
      cagtttccg accaggtta aggtgtccg gagtgttat 
      ggcgaggat gggacttgg aggaagaag gaggtgttg 
      aagtatttg aagaggatg tacgggaga tatagtgtt 
      agatatgtg gggtcggat accaaggtt ttgtttccg 
      ggacttgat agataa
    mRNA sequence
    • >TC034028 RA
      tggccatca aataaataa catcacaag cattattcc 
      tcccagtga ttacccgtc cgtcatgct caaaccact 
      agctctcct cctcgtgct agccggcag ccagtgcgc 
      ccccctagg cgacctcta cctccccga cgacgacgt 
      cgaaatcct cacaatcga gaacttcaa acgctacgt 
      cgaaaactc cacaagcgc ctggctggt ggagttcta 
      cgccagctg gtgcggcta ctgccaacg cttcgcgcc 
      cccttggaa gcagttcgc cacggaggc ggccccctg 
      gcgggacct ggtgcgggt ggcggtgtt ggagtgctc 
      cgacgagat caacacgcc gatttgtcg cgactttgg 
      aattgtcaa atacccaac agttcggta tttccacga 
      aaattcgca ctttgatgg gggcgacaa gggggttat 
      tgtgccccg ggagttccc agtgaccgt ggacgcgat 
      caaaaagaa cgtaatcga gagattcat gaccgaaat 
      gggcgaagg ccgaggggt ggtttaccc caatttgtt 
      accctacct ccattcgga cctggagcc gtttttcga 
      cgaagaaga cgacgatat tttttacgg ttttttggt 
      ggttgagga ttctgatag ttatttggg gggcgaagt 
      agcgctaga ccttcacaa aactccaaa cgttacgat 
      cagacacgc ccttaataa taacacaaa gttggtgaa 
      aaatttaca aattgggaa attcccaac acttgttat 
      aatcgatcg gaacaacaa cacgcaaat tgttaccga 
      aaatatcga acacaagaa ggagctcaa agcgacaat 
      tgccgatta cttggccaa gaaagggct caaagtttg 
      cgagaccac ccctgagaa aaagggcca cttgtccct 
      ggaccccca ccctgaccc caaacagcg ctcacgcac 
      acttttgcg gcaaaaaat caaaaaaat gggagacgc 
      cgtcttcca aatggactt ggaaacgtc ccttcggta 
      cgccctttt gcgcgaagt ctcgaccac taaagtgat 
      caaaggcga acaactggc ggcccttag ggccttcct 
      caacgtgat caagaagta tttcccctt cgggtacaa 
      ctccacgtc tttcatcaa caatttaac caacttgac 
      ttcttcgga cgaagtcca gggggtgca ggtccaggt 
      cctggtgca gcaagctga tgacagtgg ggtgttttc 
      aaccccgca gcgcttcct ggggtgtca ggggagtgc 
      gaaccgctt tcgcggata tccatgtag tctttggcg 
      ccttttcca ttacctaac agttaactc ggttctttt 
      gaatgtgag taaccggaa ggcgaatcc ggtggaagt 
      cctcggagc gatgcacgg ctacgtcaa gcacttctt 
      cagctgtag tcactgcag tgagcattt ccagaagat 
      ggccgctga gaggaatct aacgagtgt ttcgtcgct 
      tgaagagtc ggttttgtg gctctggga ggcgcataa 
      tgtggtcaa taaaagatt aaaaggtga cacaaccga 
      agatccgga gtatccgaa agaacagtt tccgaccag 
      gttaaggtg tccggagtg ttatggcga ggatgggac 
      ttggaggaa gaaggaggt gttgaagta tttgaagag 
      gatgtacgg gagatatag tgttagata tgtggggtc 
      ggataccaa ggttttgtt tccgggact tgatagata 
      acttattga cgaatttta aataaattt gttctttgt 
      tttaaaaga atcaatgtt agagaagct ttaataaat 
      acaattttt taag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY