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Gene information
General information
TC006281 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid53: 269786..280706
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006281
    iBeetle screen iB_01026
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 90.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006281 RA
      MSSSQVPAG ADPKIKEII TSLDDEKID MLAATSVLQ 
      QRATDIRAQ KVNWQSYFQ SQMISQDDH QFIAAFDVS 
      DSAKREKLL QTDRLQCAQ TFLNLLGHV SKDQTLQYI 
      LVLIDDMLQ EDRSRVEIF HEYANKKKE SVWGPFLNL 
      LNRQDGFIT NMTSRIIAK IACWSQTPM ERSDLHFYL 
      TWLKDQLKM QYDDLAVQL AKSDATRGP SGEQATETY 
      TEVSFDEQQ TPTEITNNE YIQSVGRCL QMMLRIDEY 
      RFAFVSVDG ISTLLSVLS GRVNFQVQY QLIFCLWVL 
      TFNPLLAEK MNKFNVIPI LADILSDSV KEKVTRIIL 
      AVFRNLIEK PEDAQVAKE HCIAMVQCK VLKQLNILE 
      QRKFDDEDV AGDVEFLTE KLQNSVQDL SSFDEYATE 
      VKSGRLEWS PVHKSKFWR ENAQRLNEK NYELLRILI 
      HLLETSKDP LVLSVASYD IGEYVRHYP RGKHVIEQL 
      GGKQLVMQL LAHEDPNVR YEALLAVQK LMVHNWEYL 
      GKQLEKEQS DKTAPKGGA PVAGKA
    CDS sequence
    • >TC006281 RA
      atgtcctcg tcgcaagtt cccgcaggg gcggaccct 
      aaaatcaag gagattatc acttcgctg gatgacgaa 
      aaaatagac atgctggcg gcgactagt gtcttgcag 
      cagcgggcc accgatatt cgggcccaa aaagtcaac 
      tggcagtcg tacttccag tcgcagatg atatcgcag 
      gacgaccat caatttatc gcagcgttt gatgtgtcc 
      gatagcgca aaacgcgag aaattgctc caaactgat 
      cgcctgcag tgtgcacaa accttcctc aatttgctg 
      gggcatgtc tccaaggac cagacgttg caatacatc 
      cttgtcctc atcgatgac atgctgcag gaggaccgc 
      tcgcgtgtt gaaatcttc cacgaatac gccaacaag 
      aaaaaagaa tcggtatgg gggcccttc ttgaacctg 
      ctgaaccgt caagacggc ttcatcacg aacatgacg 
      tcacgaatc atcgcgaaa atcgcctgt tggtcgcag 
      acgccaatg gagcgctcc gatttgcac ttctacctc 
      acgtggctc aaagaccag ctcaaaatg cagtacgat 
      gatttagcc gtacaattg gccaaatcg gacgcaaca 
      cgaggccca agcggtgaa caagccacc gaaacttac 
      acggaggtt tcgtttgac gaacaacaa acacctacc 
      gaaattacg aacaacgaa tacatccag tctgtgggc 
      cgttgtctc caaatgatg ctccgcatc gacgaatat 
      cgcttcgct ttcgtctca gtggacggc atttcgacc 
      cttttgtcc gtactttcg ggccgcgtc aacttccaa 
      gtgcaatac cagctgatt ttctgcctt tgggtgttg 
      actttcaat ccgctattg gccgaaaaa atgaacaaa 
      ttcaacgtg attccaatc ctggccgac attttgagc 
      gattcggtc aaggagaag gtgacccgt attattttg 
      gccgttttt cggaacctg atcgagaaa cccgaggat 
      gcgcaagtg gccaaagag cactgcatt gcgatggtg 
      cagtgcaag gttctgaag cagctaaat atcctcgaa 
      cagaggaaa ttcgacgat gaggatgtg gccggcgat 
      gtggagttt ctgactgag aagttgcaa aattccgtt 
      caggacttg agttccttc gatgagtac gcaactgag 
      gtcaagtca gggagattg gagtggtcg ccagtccac 
      aagagcaag ttttggcga gaaaatgcg caaagactc 
      aacgagaag aattacgag ttgttgagg attttgatt 
      catttgttg gagacgagc aaagatccc ctggttttg 
      agtgtcgca agttacgac ataggggaa tacgtcagg 
      cattatccc agaggaaaa cacgttatt gaacaattg 
      ggtgggaaa caactagtg atgcaattg ttggctcat 
      gaagacccc aatgttcgc tacgaggcg ctgttagct 
      gtccaaaaa ttgatggtt cataattgg gagtatttg 
      gggaaacag ttggagaag gaacagagt gataaaacg 
      gctcccaaa ggtggcgcc ccagtcgcg gggaaagcc 
      taa
    mRNA sequence
    • >TC006281 RA
      gtgtcaata atcacaaga cttgtcagg tgttgtcga 
      aaaacagga ttcaggttt agtggcgtt tttccggga 
      taaagtgcc aggttttgt gcgaaaatg tcctcgtcg 
      caagttccc gcaggggcg gaccctaaa atcaaggag 
      attatcact tcgctggat gacgaaaaa atagacatg 
      ctggcggcg actagtgtc ttgcagcag cgggccacc 
      gatattcgg gcccaaaaa gtcaactgg cagtcgtac 
      ttccagtcg cagatgata tcgcaggac gaccatcaa 
      tttatcgca gcgtttgat gtgtccgat agcgcaaaa 
      cgcgagaaa ttgctccaa actgatcgc ctgcagtgt 
      gcacaaacc ttcctcaat ttgctgggg catgtctcc 
      aaggaccag acgttgcaa tacatcctt gtcctcatc 
      gatgacatg ctgcaggag gaccgctcg cgtgttgaa 
      atcttccac gaatacgcc aacaagaaa aaagaatcg 
      gtatggggg cccttcttg aacctgctg aaccgtcaa 
      gacggcttc atcacgaac atgacgtca cgaatcatc 
      gcgaaaatc gcctgttgg tcgcagacg ccaatggag 
      cgctccgat ttgcacttc tacctcacg tggctcaaa 
      gaccagctc aaaatgcag tacgatgat ttagccgta 
      caattggcc aaatcggac gcaacacga ggcccaagc 
      ggtgaacaa gccaccgaa acttacacg gaggtttcg 
      tttgacgaa caacaaaca cctaccgaa attacgaac 
      aacgaatac atccagtct gtgggccgt tgtctccaa 
      atgatgctc cgcatcgac gaatatcgc ttcgctttc 
      gtctcagtg gacggcatt tcgaccctt ttgtccgta 
      ctttcgggc cgcgtcaac ttccaagtg caataccag 
      ctgattttc tgcctttgg gtgttgact ttcaatccg 
      ctattggcc gaaaaaatg aacaaattc aacgtgatt 
      ccaatcctg gccgacatt ttgagcgat tcggtcaag 
      gagaaggtg acccgtatt attttggcc gtttttcgg 
      aacctgatc gagaaaccc gaggatgcg caagtggcc 
      aaagagcac tgcattgcg atggtgcag tgcaaggtt 
      ctgaagcag ctaaatatc ctcgaacag aggaaattc 
      gacgatgag gatgtggcc ggcgatgtg gagtttctg 
      actgagaag ttgcaaaat tccgttcag gacttgagt 
      tccttcgat gagtacgca actgaggtc aagtcaggg 
      agattggag tggtcgcca gtccacaag agcaagttt 
      tggcgagaa aatgcgcaa agactcaac gagaagaat 
      tacgagttg ttgaggatt ttgattcat ttgttggag 
      acgagcaaa gatcccctg gttttgagt gtcgcaagt 
      tacgacata ggggaatac gtcaggcat tatcccaga 
      ggaaaacac gttattgaa caattgggt gggaaacaa 
      ctagtgatg caattgttg gctcatgaa gaccccaat 
      gttcgctac gaggcgctg ttagctgtc caaaaattg 
      atggttcat aattgggag tatttgggg aaacagttg 
      gagaaggaa cagagtgat aaaacggct cccaaaggt 
      ggcgcccca gtcgcgggg aaagcctaa attgttgtt 
      attagctcc gcttattaa atttcaagt attttattg 
      tatatatca ctagaggaa cattccggt tgaaaggat 
      gtcaggtgt tgcaaatta tagatttgt tttattgta 
      aataaatgg tattaacta tgatagtgt tgttaaaat 
      agctccttc cttccgttt atgcaatac aaagtgagt 
      taaaca