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Gene information
General information
TC006276 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6131084..6138788
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006276
    iBeetle screen iB_01025

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006276 RA
      MRKRHIWLF FLLLGFTLG EELEVKREK IATTELAHE 
      TDERLLVFA TLSGDLVGV EPQTGRIRW KIKDRPIVQ 
      VPVDTTNAI IPIFLPDPR DGSLYLMGN NREPLKKLP 
      FTIPQLVAS SPCRSSDGI FYTGKKKDT WFKLDPITG 
      RKEQVLGWD TSPTCPIES KKFVFIGRT KYDIMMVDS 
      TNKLRKWNV TFYDYTAQT MSKEEMNNY ELVHFASSS 
      TGRLVTMDR RRGNLLWDS DLGSPVVAA YVLDSDGLL 
      VAPFTSLAN HTLNYLTTE LLTHEGPQP QRMKLYPTL 
      YVGDHRYGL YAIPSLVDQ NVVTITASD GGPLLLGGP 
      HAPDLPKPY PEYPIPGYN YRLPSNMYE GDPITFQSI 
      PNFVIYVGH YNVPKYTDT KFLLGGGTP KVNLLTDES 
      ESNETPPPK TVPKSIEES DQQQPQTNK NRIKSYYKS 
      VKLWVNQQE NKGLKLVLI ILVGCVIAM FWYLQVQVR 
      EFQNMSQNG SRSSQQNSF GKNSSITAY SEELPDGLV 
      RVGKITFHP DQLLGKGCE GTFVYRGEF DSRQVAVKR 
      LLPECFTFA DREVALLRE SDAHPNVIR YYCMEQDRL 
      FRYIALELC QATLSEYVQ GKCDRTMIK PLDILRQAT 
      SGLGHLHSL DIVHRDIKP HNVLLSVPN SQGEVKAMI 
      SDFGLCKKL QVGRVSFSR RSGVTGTDG WIAPEMLNG 
      NERTTCAVD LFSLGCLFY YVLSNGLHP FGDNLRRQA 
      NILSGDYNL DDLQGEEWQ INLQKPLLA AMISSKPNE 
      RPSCSAILK HPMFWDNAK ILAFFQDVS DRVEKAETD 
      DSVLQNLEE MNFTIVRTD WRTHIHEEV ATDLRKYRS 
      YRGESVRDL LRALRNKKH HFRELTKEA QSLLGEIPD 
      SFTSYWTKR FPLLLVHSW LAMQCVSDE TAFQPYYHD 
      TYKYSYQNF LQQIQNYIV EKPDFYEFP KMKNRLFDT 
      NPRKVRENV GLYRNLAPQ EVVDVNEVN VTITDRRFK 
      KSEVRIRSK KRASKRDEP LVWAIRQDK
    CDS sequence
    • >TC006276 RA
      atgcgcaaa cgtcacatt tggttattt ttccttctt 
      ttgggtttc accctcggc gaggaactt gaagtaaaa 
      cgcgagaaa atcgccacc actgagctg gcacacgag 
      accgacgaa agactccta gtttttgcg actttaagc 
      ggcgatttg gtcggtgtt gagccccaa acgggcaga 
      atccgatgg aaaatcaaa gacagacct attgttcag 
      gtgcctgtc gacaccaca aacgccata atcccgatt 
      tttctgccc gatcccagg gacggttct ttgtactta 
      atggggaat aaccgggaa cctttaaag aaattaccc 
      ttcactatt ccacaattg gtggcctcg tcgccttgt 
      cgcagttct gatgggatt ttctatacc gggaaaaag 
      aaggacacg tggtttaaa cttgaccca atcactggg 
      agaaaagag caagtttta ggctgggat accagtccc 
      acgtgtccc attgaatcg aaaaaattc gtttttatc 
      ggacgcacg aaatacgac attatgatg gtcgatagt 
      accaataaa ttgcgcaaa tggaacgtc acgttttac 
      gattatacg gcgcaaaca atgtcgaag gaagaaatg 
      aacaattac gaattggta cattttgcg tcaagttcc 
      accggtcgc ttggtaacc atggaccgt cgccgtggc 
      aacctcctc tgggacagc gatctggga agtcccgtg 
      gtggcagca tacgtactt gattcagac gggttatta 
      gtggccccg tttacaagc ctagccaac cacactttg 
      aattatcta acgacggaa ttgttgact cacgagggg 
      ccccaaccc caacgaatg aaactatat cccactctg 
      tacgtgggt gatcatagg tacggcctt tatgccatt 
      ccgtctctc gttgaccaa aatgtggtc acgatcaca 
      gcttcagat gggggccct ttgttacta gggggcccc 
      catgcgccc gatttgccc aaaccgtac cccgagtac 
      cccataccg gggtataac tataggcta ccctcaaat 
      atgtacgaa ggtgacccg attacgttc caaagcatc 
      ccaaatttt gttatatac gttggtcac tacaacgtg 
      ccaaaatac accgatacg aaatttctg ttaggtggg 
      ggtacccct aaagtgaac cttttgacc gacgaaagt 
      gagtcaaat gagacacct cccccaaaa accgtcccc 
      aagtcaata gaagaaagc gaccaacaa cagccccaa 
      actaataaa aatcgtatc aaatcttac tacaaatcg 
      gtgaaatta tgggtaaac cagcaggaa aacaagggt 
      ttgaagcta gttttgatc attttggtg ggttgcgtt 
      atcgccatg ttttggtac ttgcaagtg caagtgcgc 
      gagttccaa aacatgtcc cagaatggc tcacgctca 
      agtcagcaa aactccttt gggaaaaat tcctcaatc 
      accgcctat tccgaggaa ttacccgat gggttggtc 
      cgtgtgggt aaaataacc ttccatccg gaccaactc 
      ctgggtaaa ggttgcgaa ggtactttt gtctatcgg 
      ggtgaattc gatagtaga caagtcgcc gtcaagcga 
      ctacttccc gaatgtttc acgttcgcc gaccgggaa 
      gttgccctt ttaagggaa agtgatgcc catcccaat 
      gttataagg tactactgc atggaacaa gaccgattg 
      ttcaggtac atagccctt gagttgtgc caagcgact 
      ttatcggaa tacgtgcaa gggaaatgc gacaggacg 
      atgattaaa ccgttggat atcctgaga caggccact 
      tcgggcctt ggacatttg cattcgctc gatattgtc 
      caccgtgat attaagccc cacaatgtt ctgctttcc 
      gtccccaat agtcaaggc gaagtcaaa gcaatgatc 
      tcagatttc ggtttatgt aaaaaactc caagtcggg 
      agagtttca ttttcgaga cgttccgga gtcaccgga 
      accgacggt tggattgcc ccggaaatg ctcaacggc 
      aacgagaga accacttgt gctgtggat ttattttcg 
      ctcggttgt ctcttttac tacgttctt tcgaacggt 
      ttgcaccct tttggtgat aatttacga cgacaagcg 
      aatattttg agcggtgat tataatttg gacgatctt 
      caaggtgaa gaatggcaa attaatcta cagaaacct 
      ttactagcg gcgatgatt tcgtcgaaa ccgaacgaa 
      cggccttct tgcagtgcc attttaaaa cacccaatg 
      ttttgggac aatgcgaaa attttagcc tttttccaa 
      gatgtcagc gatagggtc gaaaaggcc gaaaccgat 
      gactcagtt ttacaaaat ttagaagaa atgaatttt 
      acgatagtc aggactgac tggcgaacg cacattcat 
      gaagaagtc gccacagat ttgcgaaaa tatcgctcg 
      tatcgaggg gaatcggtc cgggatctt ctacgagca 
      cttagaaac aaaaaacat cactttcgg gagttgacc 
      aaagaggcg cagagcctt ttgggcgaa atccccgac 
      tcctttacg agttactgg acgaagcgg tttcccctg 
      ttactggtg cattcgtgg ttggccatg caatgcgtt 
      tcggacgaa acggccttt cagccttac taccacgac 
      acttacaaa tattcctat caaaatttc ctacaacag 
      attcaaaac tacatcgtg gaaaagccc gatttttat 
      gaatttcca aaaatgaaa aatcgtcta tttgacaca 
      aacccgaga aaagttcgt gaaaatgtg ggactttac 
      cggaacttg gcgccacaa gaagtcgta gatgtgaac 
      gaggtcaat gttacgata actgatcgt aggtttaag 
      aaaagtgaa gtacggatt aggagcaag aaacgggcc 
      tcaaaacga gacgagccg ctagtctgg gccataaga 
      caagataaa taa
    mRNA sequence
    • >TC006276 RA
      taaatacaa ataatggct taaaccgat tattttccc 
      cgaaatttg gctttcaaa gcttacaat tgtacgatt 
      ttatgtgaa ttcgtgacc ttcaaaatg cgcaaacgt 
      cacatttgg ttatttttc cttcttttg ggtttcacc 
      ctcggcgag gaacttgaa gtaaaacgc gagaaaatc 
      gccaccact gagctggca cacgagacc gacgaaaga 
      ctcctagtt tttgcgact ttaagcggc gatttggtc 
      ggtgttgag ccccaaacg ggcagaatc cgatggaaa 
      atcaaagac agacctatt gttcaggtg cctgtcgac 
      accacaaac gccataatc ccgattttt ctgcccgat 
      cccagggac ggttctttg tacttaatg gggaataac 
      cgggaacct ttaaagaaa ttacccttc actattcca 
      caattggtg gcctcgtcg ccttgtcgc agttctgat 
      gggattttc tataccggg aaaaagaag gacacgtgg 
      tttaaactt gacccaatc actgggaga aaagagcaa 
      gttttaggc tgggatacc agtcccacg tgtcccatt 
      gaatcgaaa aaattcgtt tttatcgga cgcacgaaa 
      tacgacatt atgatggtc gatagtacc aataaattg 
      cgcaaatgg aacgtcacg ttttacgat tatacggcg 
      caaacaatg tcgaaggaa gaaatgaac aattacgaa 
      ttggtacat tttgcgtca agttccacc ggtcgcttg 
      gtaaccatg gaccgtcgc cgtggcaac ctcctctgg 
      gacagcgat ctgggaagt cccgtggtg gcagcatac 
      gtacttgat tcagacggg ttattagtg gccccgttt 
      acaagccta gccaaccac actttgaat tatctaacg 
      acggaattg ttgactcac gaggggccc caaccccaa 
      cgaatgaaa ctatatccc actctgtac gtgggtgat 
      cataggtac ggcctttat gccattccg tctctcgtt 
      gaccaaaat gtggtcacg atcacagct tcagatggg 
      ggccctttg ttactaggg ggcccccat gcgcccgat 
      ttgcccaaa ccgtacccc gagtacccc ataccgggg 
      tataactat aggctaccc tcaaatatg tacgaaggt 
      gacccgatt acgttccaa agcatccca aattttgtt 
      atatacgtt ggtcactac aacgtgcca aaatacacc 
      gatacgaaa tttctgtta ggtgggggt acccctaaa 
      gtgaacctt ttgaccgac gaaagtgag tcaaatgag 
      acacctccc ccaaaaacc gtccccaag tcaatagaa 
      gaaagcgac caacaacag ccccaaact aataaaaat 
      cgtatcaaa tcttactac aaatcggtg aaattatgg 
      gtaaaccag caggaaaac aagggtttg aagctagtt 
      ttgatcatt ttggtgggt tgcgttatc gccatgttt 
      tggtacttg caagtgcaa gtgcgcgag ttccaaaac 
      atgtcccag aatggctca cgctcaagt cagcaaaac 
      tcctttggg aaaaattcc tcaatcacc gcctattcc 
      gaggaatta cccgatggg ttggtccgt gtgggtaaa 
      ataaccttc catccggac caactcctg ggtaaaggt 
      tgcgaaggt acttttgtc tatcggggt gaattcgat 
      agtagacaa gtcgccgtc aagcgacta cttcccgaa 
      tgtttcacg ttcgccgac cgggaagtt gccctttta 
      agggaaagt gatgcccat cccaatgtt ataaggtac 
      tactgcatg gaacaagac cgattgttc aggtacata 
      gcccttgag ttgtgccaa gcgacttta tcggaatac 
      gtgcaaggg aaatgcgac aggacgatg attaaaccg 
      ttggatatc ctgagacag gccacttcg ggccttgga 
      catttgcat tcgctcgat attgtccac cgtgatatt 
      aagccccac aatgttctg ctttccgtc cccaatagt 
      caaggcgaa gtcaaagca atgatctca gatttcggt 
      ttatgtaaa aaactccaa gtcgggaga gtttcattt 
      tcgagacgt tccggagtc accggaacc gacggttgg 
      attgccccg gaaatgctc aacggcaac gagagaacc 
      acttgtgct gtggattta ttttcgctc ggttgtctc 
      ttttactac gttctttcg aacggtttg caccctttt 
      ggtgataat ttacgacga caagcgaat attttgagc 
      ggtgattat aatttggac gatcttcaa ggtgaagaa 
      tggcaaatt aatctacag aaaccttta ctagcggcg 
      atgatttcg tcgaaaccg aacgaacgg ccttcttgc 
      agtgccatt ttaaaacac ccaatgttt tgggacaat 
      gcgaaaatt ttagccttt ttccaagat gtcagcgat 
      agggtcgaa aaggccgaa accgatgac tcagtttta 
      caaaattta gaagaaatg aattttacg atagtcagg 
      actgactgg cgaacgcac attcatgaa gaagtcgcc 
      acagatttg cgaaaatat cgctcgtat cgaggggaa 
      tcggtccgg gatcttcta cgagcactt agaaacaaa 
      aaacatcac tttcgggag ttgaccaaa gaggcgcag 
      agccttttg ggcgaaatc cccgactcc tttacgagt 
      tactggacg aagcggttt cccctgtta ctggtgcat 
      tcgtggttg gccatgcaa tgcgtttcg gacgaaacg 
      gcctttcag ccttactac cacgacact tacaaatat 
      tcctatcaa aatttccta caacagatt caaaactac 
      atcgtggaa aagcccgat ttttatgaa tttccaaaa 
      atgaaaaat cgtctattt gacacaaac ccgagaaaa 
      gttcgtgaa aatgtggga ctttaccgg aacttggcg 
      ccacaagaa gtcgtagat gtgaacgag gtcaatgtt 
      acgataact gatcgtagg tttaagaaa agtgaagta 
      cggattagg agcaagaaa cgggcctca aaacgagac 
      gagccgcta gtctgggcc ataagacaa gataaataa 
      gtacaattt tgttatttt ttggtaatt ttaaggaat 
      cactgtgat aattgtagc actttgttt ttaaggacc 
      aattaatgt taatcgttg ccaaatata ttttct
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY