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Gene information
General information
TC006222 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 5041686..5046945
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006222
    iBeetle screen iB_01019
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006222 RA
      MRSLEVSTP WQEEEDVEV EDIELEKHA 
      MNGRDDLTE SSSDEATGR KRRKHGGSA 
      SSGSASSGT TTRRRKSCI SARERNLRR 
      LESNERERM RMHSLNDAF EQLREVIPH 
      IKMERKLSK IETLTLAKN YIMALTNVI 
      CEMRGEEKH YTFQDVEDE EVELETTEE 
      PNREQNNNS MFQQNLENH QENAMAYVG 
      WL
    • >TC006222 RB
      MRSLEVSTP WQEEEDVEV EDIELEKHA 
      MNGRDDLTE SSSDEATGR KRRKHGGSA 
      SSGSASSGT TTRRRKSCI SARERNLRR 
      LESNERERM RMHSLNDAF EQLREVIPH 
      IKMERKLSK IETLTLAKN YIMALTNVI 
      CEMRGEEKH YTFQDVEDE EVELETTEE 
      PNREQNNNS MFQQNLENH QENAMVNSR
    CDS sequence
    • >TC006222 RA
      atgcggtcg ttggaagtg tcgactcct tggcaggag 
      gaggaggat gtggaggtg gaggacatc gagctggag 
      aagcacgcg atgaacggg agagacgat ctgaccgag 
      tcgagtagt gacgaagca acgggaaga aagcgaaga 
      aagcatgga gggagtgct tcgagtgga agtgcgtcg 
      agcgggacg acgacgagg aggaggaag tcgtgcatc 
      agtgcgcga gagagaaat ttaagaaga ctggagagc 
      aatgagagg gaaagaatg aggatgcat tcactcaac 
      gatgcgttc gagcaatta agagaagtc atcccgcat 
      ataaaaatg gagcgcaaa ctatccaaa atcgagact 
      ttaacctta gccaaaaac tacataatg gccctaacg 
      aacgtgata tgcgagatg cgaggcgag gagaaacac 
      tacacattt caagacgtg gaggacgag gaggtggag 
      ctggagacg acagaagag ccaaaccgg gagcagaac 
      aacaactca atgtttcag caaaacctc gaaaaccac 
      caagagaac gcgatggcc tacgttggg tggttgtaa
    • >TC006222 RB
      atgcggtcg ttggaagtg tcgactcct tggcaggag 
      gaggaggat gtggaggtg gaggacatc gagctggag 
      aagcacgcg atgaacggg agagacgat ctgaccgag 
      tcgagtagt gacgaagca acgggaaga aagcgaaga 
      aagcatgga gggagtgct tcgagtgga agtgcgtcg 
      agcgggacg acgacgagg aggaggaag tcgtgcatc 
      agtgcgcga gagagaaat ttaagaaga ctggagagc 
      aatgagagg gaaagaatg aggatgcat tcactcaac 
      gatgcgttc gagcaatta agagaagtc atcccgcat 
      ataaaaatg gagcgcaaa ctatccaaa atcgagact 
      ttaacctta gccaaaaac tacataatg gccctaacg 
      aacgtgata tgcgagatg cgaggcgag gagaaacac 
      tacacattt caagacgtg gaggacgag gaggtggag 
      ctggagacg acagaagag ccaaaccgg gagcagaac 
      aacaactca atgtttcag caaaacctc gaaaaccac 
      caagagaac gcgatggtg aattcccgc tga
    mRNA sequence
    • >TC006222 RA
      atgcacgta cacgtacaa acttcaccg aaacgacga 
      tgaggggtt ttacagtgc tgtcaagtg aaacggcga 
      cattttgag caagtgatt tttcggaac gtggtgata 
      gaatgcggt cgttggaag tgtcgactc cttggcagg 
      aggaggagg atgtggagg tggaggaca tcgagctgg 
      agaagcacg cgatgaacg ggagagacg atctgaccg 
      agtcgagta gtgacgaag caacgggaa gaaagcgaa 
      gaaagcatg gagggagtg cttcgagtg gaagtgcgt 
      cgagcggga cgacgacga ggaggagga agtcgtgca 
      tcagtgcgc gagagagaa atttaagaa gactggaga 
      gcaatgaga gggaaagaa tgaggatgc attcactca 
      acgatgcgt tcgagcaat taagagaag tcatcccgc 
      atataaaaa tggagcgca aactatcca aaatcgaga 
      ctttaacct tagccaaaa actacataa tggccctaa 
      cgaacgtga tatgcgaga tgcgaggcg aggagaaac 
      actacacat ttcaagacg tggaggacg aggaggtgg 
      agctggaga cgacagaag agccaaacc gggagcaga 
      acaacaact caatgtttc agcaaaacc tcgaaaacc 
      accaagaga acgcgatgg cctacgttg ggtggttgt 
      aattttctt gataaagct aatgtgatt taatgtgct 
      tccgtgttg tgtccgtgt atagatagt gataagtat 
      agtgcatct agagacgta aaagacact tatttttaa 
      acaagtata gattgtgtt taaattatt ttatatgaa 
      atgacataa ctaccttga cttgttata ttagagtac 
      ctagatatg taagataaa gtgtcgttt tgtttggtt 
      tttgacgta atatggaag ctgcgaacc ggcgagctt 
      gcagcttcc ataccggga aatgacacg aacaaagtt 
      acctaaacc gaggttgga acctcaaaa tactctaag 
      ttatgctca agaatgtca gtgcgaatt gttcaccga 
      ctgtcatca tagtttcgc ttctcaaaa gtgagcagt 
      tcgtacgga aggaagcga ttggaagtt ccaaatgca 
      aatgtgata ttgtagaaa acttgcaca agtttttgt 
      tagaacata taaacatat attttatga cgtgtatta 
      agtgtattc ttggacaaa atattacac ttcgtgaga 
      gatatacat tccaatatc aaataaaat gtcaggaat 
      ctt
    • >TC006222 RB
      atgcacgta cacgtacaa acttcaccg aaacgacga 
      tgaggggtt ttacagtgc tgtcaagtg aaacggcga 
      cattttgag caagtgatt tttcggaac gtggtgata 
      gaatgcggt cgttggaag tgtcgactc cttggcagg 
      aggaggagg atgtggagg tggaggaca tcgagctgg 
      agaagcacg cgatgaacg ggagagacg atctgaccg 
      agtcgagta gtgacgaag caacgggaa gaaagcgaa 
      gaaagcatg gagggagtg cttcgagtg gaagtgcgt 
      cgagcggga cgacgacga ggaggagga agtcgtgca 
      tcagtgcgc gagagagaa atttaagaa gactggaga 
      gcaatgaga gggaaagaa tgaggatgc attcactca 
      acgatgcgt tcgagcaat taagagaag tcatcccgc 
      atataaaaa tggagcgca aactatcca aaatcgaga 
      ctttaacct tagccaaaa actacataa tggccctaa 
      cgaacgtga tatgcgaga tgcgaggcg aggagaaac 
      actacacat ttcaagacg tggaggacg aggaggtgg 
      agctggaga cgacagaag agccaaacc gggagcaga 
      acaacaact caatgtttc agcaaaacc tcgaaaacc 
      accaagaga acgcgatgg tgaattccc gctgaactc 
      gcgattgat tctgattgt gcatatctg ttctaggcc 
      tacgttggg tggttgtaa ttttcttga taaagctaa 
      tgtgattta atgtgcttc cgtgttgtg tccgtgtat 
      agatagtga taagtatag tgcatctag agacgtaaa 
      agacactta tttttaaac aagtataga ttgtgttta 
      aattatttt atatgaaat gacataact accttgact 
      tgttatatt agagtacct agatatgta agataaagt 
      gtcgttttg tttggtttt tgacgtaat atggaagct 
      gcgaaccgg cgagcttgc agcttccat accgggaaa 
      tgacacgaa caaagttac ctaaaccga ggttggaac 
      ctcaaaata ctctaagtt atgctcaag aatgtcagt 
      gcgaattgt tcaccgact gtcatcata gtttcgctt 
      ctcaaaagt gagcagttc gtacggaag gaagcgatt 
      ggaagttcc aaatgcaaa tgtgatatt gtagaaaac 
      ttgcacaag tttttgtta gaacatata aacatatat 
      tttatgacg tgtattaag tgtattctt ggacaaaat 
      attacactt cgtgagaga tatacattc caatatcaa 
      ataaaatgt caggaatct t