Status
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Gene information
General information
TC006219 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5213126..5220889
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006219
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01018

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with muscle fluorescence/pattern, but not hatched (pot. late embryonic lethality) - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants without visible segmentation, differentiation or axis - Penetrance (percentage of animals/eggs on slide): less than 30%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    labrum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006219 RA
      MTSNKVELY IYDLSKGMA AALSPMLIN KKIDGIWHT 
AIVVYGREY FFGSHGISS CNPGTTALG QPLRVLTLG 
ETQVPYSVF IDYINGLSE STWAGPTYD LFHHNCNNF 
SEEIAQFLC GCSIPKYIL DLPNEVLSS SLGPAIPLL 
VSQLEKSAR PIAEEQERA RENSPDLEQ LNSQIEEVR 
YRSFLLEQR RKTIKEKLA KKDRKKEKK RKKLQSQGS 
AIPVELEEV AMAEPEVNG AESRLPTDQ VMDLEEEER 
REEEEKKKS REPPVVYKD LLDAKTEFD ALLGLVDGK 
LSEEEQRSM EELQVYMIG DEGSWALSD GFLTFIGRL 
LHDKNLPNE VRVRTLNIL ALAALKDDV ILVLHQDRK 
DHVLMNYAF DIDRHSPEE QEALALFVA NMFENLSSS 
EWLMYISEW SYNNQQTSN IRVTTKVAV HSLLSDLPL 
LQERGTAII HNLACKEVK SVVCICILS SALAIIYGK 
KLNKYELFK ILLEYP
    • >TC006219 RB
      MTSNKVELY IYDLSKGMA AALSPMLIN KKIDGIWHT 
AIVVYGREY FFGSHGISS CNPIFGTTA LGQPLRVLT 
LGETQVPYS VFIDYINGL SESTWAGPT YDLFHHNCN 
NFSEEIAQF LCGCSIPKY ILDLPNEVL SSSLGPAIP 
LLVSQLEKS ARPIAEEQE RARENSPDL EQLNSQIEE 
VRYRSFLLE QRRKTIKEK LAKKDRKKE KKRKKLQSQ 
GSAIPVELE EVAMAEPEV NGAESRLPT DQVMDLEEE 
ERREEEEKK KSREPPVVY KDLLDAKTE FDALLGLVD 
GKLSEEEQR SMEELQVYM IGDEGSWAL SDGFLTFIG 
RLLHDKNLP NEVRVRTLN ILALAALKD DVILVLHQD 
RKDHVLMNY AFDIDRHSP EEQEALALF VANMFENLS 
SSEWLMYIS EWSYNNQQT SNIRVTTKV AVHSLLSDL 
PLLQERGTA IIHNLACKE VKSVVCICI LSSALAIIY 
GKKLNKYEL FKILLEYP
    CDS sequence
    • >TC006219 RA
      atgacctca aacaaggtg gagttgtac atttacgac 
ctgtccaaa gggatggca gcggccctt tcgcctatg 
ctcataaac aaaaaaatc gacggaatt tggcacaca 
gcgattgtc gtttacggt cgcgagtat tttttcggc 
tcacatggc atttccagt tgtaatccg ggtacgacc 
gctttgggg cagcccttg cgcgtgtta actcttggc 
gagacccag gtcccttat tcagtattc atcgattac 
ataaacggt ctcagcgaa tcaacttgg gcgggccca 
acttacgat ctttttcac cacaactgc aacaacttc 
tcggaggaa atcgcccag tttttgtgc ggttgtagc 
atcccgaaa tatatcctc gatttacca aacgaggtt 
cttagttcg tctctagga ccagcgata ccgcttttg 
gtgtcacag ttggagaaa agtgcaaga cctatagcc 
gaagaacag gaaagggcg agggagaat agccccgat 
ttggaacag ttgaattca caaattgaa gaagtcagg 
taccggtca tttttgctc gaacagcgc cgcaaaaca 
atcaaagaa aagctcgct aagaaagac cggaagaag 
gaaaaaaaa cgcaagaag ttgcaaagt caaggtagt 
gcaatcccg gtcgaatta gaagaagtc gcaatggcc 
gaacctgaa gtaaacggc gccgagagt cggctacca 
accgaccaa gtcatggat ttggaagag gaagaaaga 
cgcgaagaa gaagaaaag aaaaaatcg cgggaaccc 
ccggtcgtc tacaaagac ctacttgat gccaaaacc 
gagttcgac gctcttctg ggtttggtc gatgggaag 
ttgtccgaa gaggagcag cgatcgatg gaagagctg 
caagtttac atgattggg gatgagggg agttgggcc 
ttaagtgac gggtttttg acttttatt gggcgtttg 
cttcacgac aaaaacttg ccaaatgag gtgcgtgtc 
cgcactttg aatattctg gcccttgct gcgttgaag 
gacgatgtc attttggtt ttgcatcag gatcgcaaa 
gaccatgtt ttgatgaac tatgccttt gatattgat 
aggcacagc cctgaggag caggaggct ttggctctt 
tttgttgcc aacatgttt gaaaatttg agttcgtca 
gaatggctg atgtatatc tcggagtgg agttacaat 
aaccaacaa actagcaat attagggtc actactaaa 
gtggcagtt cattcgttg ttgtccgac ttgcctctc 
ctgcaagaa cggggcact gcgatcatc cataatctc 
gcctgcaag gaggtaaaa tcagtggtc tgtatctgt 
atcttatct tcagcttta gcgattatc tacgggaaa 
aaattaaac aaatatgaa ttatttaaa atcctatta 
gaatatcct tag
    • >TC006219 RB
      atgacctca aacaaggtg gagttgtac atttacgac 
ctgtccaaa gggatggca gcggccctt tcgcctatg 
ctcataaac aaaaaaatc gacggaatt tggcacaca 
gcgattgtc gtttacggt cgcgagtat tttttcggc 
tcacatggc atttccagt tgtaatccg atctttggt 
acgaccgct ttggggcag cccttgcgc gtgttaact 
cttggcgag acccaggtc ccttattca gtattcatc 
gattacata aacggtctc agcgaatca acttgggcg 
ggcccaact tacgatctt tttcaccac aactgcaac 
aacttctcg gaggaaatc gcccagttt ttgtgcggt 
tgtagcatc ccgaaatat atcctcgat ttaccaaac 
gaggttctt agttcgtct ctaggacca gcgataccg 
cttttggtg tcacagttg gagaaaagt gcaagacct 
atagccgaa gaacaggaa agggcgagg gagaatagc 
cccgatttg gaacagttg aattcacaa attgaagaa 
gtcaggtac cggtcattt ttgctcgaa cagcgccgc 
aaaacaatc aaagaaaag ctcgctaag aaagaccgg 
aagaaggaa aaaaaacgc aagaagttg caaagtcaa 
ggtagtgca atcccggtc gaattagaa gaagtcgca 
atggccgaa cctgaagta aacggcgcc gagagtcgg 
ctaccaacc gaccaagtc atggatttg gaagaggaa 
gaaagacgc gaagaagaa gaaaagaaa aaatcgcgg 
gaacccccg gtcgtctac aaagaccta cttgatgcc 
aaaaccgag ttcgacgct cttctgggt ttggtcgat 
gggaagttg tccgaagag gagcagcga tcgatggaa 
gagctgcaa gtttacatg attggggat gaggggagt 
tgggcctta agtgacggg tttttgact tttattggg 
cgtttgctt cacgacaaa aacttgcca aatgaggtg 
cgtgtccgc actttgaat attctggcc cttgctgcg 
ttgaaggac gatgtcatt ttggttttg catcaggat 
cgcaaagac catgttttg atgaactat gcctttgat 
attgatagg cacagccct gaggagcag gaggctttg 
gctcttttt gttgccaac atgtttgaa aatttgagt 
tcgtcagaa tggctgatg tatatctcg gagtggagt 
tacaataac caacaaact agcaatatt agggtcact 
actaaagtg gcagttcat tcgttgttg tccgacttg 
cctctcctg caagaacgg ggcactgcg atcatccat 
aatctcgcc tgcaaggag gtaaaatca gtggtctgt 
atctgtatc ttatcttca gctttagcg attatctac 
gggaaaaaa ttaaacaaa tatgaatta tttaaaatc 
ctattagaa tatccttag
    mRNA sequence
    • >TC006219 RA
      ccgagcctg cagcgcgtt tcgcaacat aattaattt 
gtgcgtggt tttaatcaa ttgtagtca aaatcagtt 
cgctagttt ttgaaaaaa atccagtta aaaaaatac 
ccaaatttg gtgataaac cggaacccg gagaacccg 
gagtcacct gtcaaatcg tcgaagatt ccagatctc 
tgtgtctgt gtcattata aaacggttc aaatcttgc 
gattagtta gtgagttag cagaattat tgcgccaaa 
atgacctca aacaaggtg gagttgtac atttacgac 
ctgtccaaa gggatggca gcggccctt tcgcctatg 
ctcataaac aaaaaaatc gacggaatt tggcacaca 
gcgattgtc gtttacggt cgcgagtat tttttcggc 
tcacatggc atttccagt tgtaatccg ggtacgacc 
gctttgggg cagcccttg cgcgtgtta actcttggc 
gagacccag gtcccttat tcagtattc atcgattac 
ataaacggt ctcagcgaa tcaacttgg gcgggccca 
acttacgat ctttttcac cacaactgc aacaacttc 
tcggaggaa atcgcccag tttttgtgc ggttgtagc 
atcccgaaa tatatcctc gatttacca aacgaggtt 
cttagttcg tctctagga ccagcgata ccgcttttg 
gtgtcacag ttggagaaa agtgcaaga cctatagcc 
gaagaacag gaaagggcg agggagaat agccccgat 
ttggaacag ttgaattca caaattgaa gaagtcagg 
taccggtca tttttgctc gaacagcgc cgcaaaaca 
atcaaagaa aagctcgct aagaaagac cggaagaag 
gaaaaaaaa cgcaagaag ttgcaaagt caaggtagt 
gcaatcccg gtcgaatta gaagaagtc gcaatggcc 
gaacctgaa gtaaacggc gccgagagt cggctacca 
accgaccaa gtcatggat ttggaagag gaagaaaga 
cgcgaagaa gaagaaaag aaaaaatcg cgggaaccc 
ccggtcgtc tacaaagac ctacttgat gccaaaacc 
gagttcgac gctcttctg ggtttggtc gatgggaag 
ttgtccgaa gaggagcag cgatcgatg gaagagctg 
caagtttac atgattggg gatgagggg agttgggcc 
ttaagtgac gggtttttg acttttatt gggcgtttg 
cttcacgac aaaaacttg ccaaatgag gtgcgtgtc 
cgcactttg aatattctg gcccttgct gcgttgaag 
gacgatgtc attttggtt ttgcatcag gatcgcaaa 
gaccatgtt ttgatgaac tatgccttt gatattgat 
aggcacagc cctgaggag caggaggct ttggctctt 
tttgttgcc aacatgttt gaaaatttg agttcgtca 
gaatggctg atgtatatc tcggagtgg agttacaat 
aaccaacaa actagcaat attagggtc actactaaa 
gtggcagtt cattcgttg ttgtccgac ttgcctctc 
ctgcaagaa cggggcact gcgatcatc cataatctc 
gcctgcaag gaggtaaaa tcagtggtc tgtatctgt 
atcttatct tcagcttta gcgattatc tacgggaaa 
aaattaaac aaatatgaa ttatttaaa atcctatta 
gaatatcct tagctccac aagccaaaa ttttttaac 
tatttaaat aaattcata aaaccaata aatttaaag 
gtaatcaat gtaacatta ataaaagga attctcgaa 
ccctacaag aaaagtaag aataacatc ctgaataca 
aacaaccat gttgactga aagagtata aaaacaatg 
aatatacag ccccaaaat tt
    • >TC006219 RB
      ccgagcctg cagcgcgtt tcgcaacat aattaattt 
gtgcgtggt tttaatcaa ttgtagtca aaatcagtt 
cgctagttt ttgaaaaaa atccagtta aaaaaatac 
ccaaatttg gtgataaac cggaacccg gagaacccg 
gagtcacct gtcaaatcg tcgaagatt ccagatctc 
tgtgtctgt gtcattata aaacggttc aaatcttgc 
gattagtta gtgagttag cagaattat tgcgccaaa 
atgacctca aacaaggtg gagttgtac atttacgac 
ctgtccaaa gggatggca gcggccctt tcgcctatg 
ctcataaac aaaaaaatc gacggaatt tggcacaca 
gcgattgtc gtttacggt cgcgagtat tttttcggc 
tcacatggc atttccagt tgtaatccg atctttggt 
acgaccgct ttggggcag cccttgcgc gtgttaact 
cttggcgag acccaggtc ccttattca gtattcatc 
gattacata aacggtctc agcgaatca acttgggcg 
ggcccaact tacgatctt tttcaccac aactgcaac 
aacttctcg gaggaaatc gcccagttt ttgtgcggt 
tgtagcatc ccgaaatat atcctcgat ttaccaaac 
gaggttctt agttcgtct ctaggacca gcgataccg 
cttttggtg tcacagttg gagaaaagt gcaagacct 
atagccgaa gaacaggaa agggcgagg gagaatagc 
cccgatttg gaacagttg aattcacaa attgaagaa 
gtcaggtac cggtcattt ttgctcgaa cagcgccgc 
aaaacaatc aaagaaaag ctcgctaag aaagaccgg 
aagaaggaa aaaaaacgc aagaagttg caaagtcaa 
ggtagtgca atcccggtc gaattagaa gaagtcgca 
atggccgaa cctgaagta aacggcgcc gagagtcgg 
ctaccaacc gaccaagtc atggatttg gaagaggaa 
gaaagacgc gaagaagaa gaaaagaaa aaatcgcgg 
gaacccccg gtcgtctac aaagaccta cttgatgcc 
aaaaccgag ttcgacgct cttctgggt ttggtcgat 
gggaagttg tccgaagag gagcagcga tcgatggaa 
gagctgcaa gtttacatg attggggat gaggggagt 
tgggcctta agtgacggg tttttgact tttattggg 
cgtttgctt cacgacaaa aacttgcca aatgaggtg 
cgtgtccgc actttgaat attctggcc cttgctgcg 
ttgaaggac gatgtcatt ttggttttg catcaggat 
cgcaaagac catgttttg atgaactat gcctttgat 
attgatagg cacagccct gaggagcag gaggctttg 
gctcttttt gttgccaac atgtttgaa aatttgagt 
tcgtcagaa tggctgatg tatatctcg gagtggagt 
tacaataac caacaaact agcaatatt agggtcact 
actaaagtg gcagttcat tcgttgttg tccgacttg 
cctctcctg caagaacgg ggcactgcg atcatccat 
aatctcgcc tgcaaggag gtaaaatca gtggtctgt 
atctgtatc ttatcttca gctttagcg attatctac 
gggaaaaaa ttaaacaaa tatgaatta tttaaaatc 
ctattagaa tatccttag ctccacaag ccaaaattt 
tttaactat ttaaataaa ttcataaaa ccaataaat 
ttaaaggta atcaatgta acattaata aaaggaatt 
ctcgaaccc tacaagaaa agtaagaat aacatcctg 
aatacaaac aaccatgtt gactgaaag agtataaaa 
acaatgaat atacagccc caaaattt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY