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Gene information
General information
TC006217 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5179078..5192167
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006217
    iBeetle screen iB_01017

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber not present - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 6 (11 dpi)
    Days post injection


    pupal molt delayed (still prepupa) - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection



    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006217 RA
      MSNITSSDS NGTKNYFVN YNQDSTSLV LGSKSGYRL 
      YSLNNVDDL ELIYSNDVE ETYMVERLF SSSLVATVA 
      LSSPRKLKV CHFKKGTEI CNYSYSNTI LAVRMNRSR 
      LVVCLEESL YIHNIRDMK VLHTIRDTP PNPQGLCAL 
      SINHDNCLL AYPGSATIG EVQIFDADH LHAKTMIPA 
      HDSPLAALA FSPNGTRIA TASEKGTVI RVFSSADGQ 
      KLYEFRRGV KRCVDISSL AFSTCSQFL CCSSNTETV 
      HVFKFEEPK ESPKRNVEE SSWMGYLSS YLPTQVTDV 
      FTQGRAFAA AHLPTQGLR NVISIMTAQ NQLRLLVAT 
      EEAKLYVYN LDPNEGGDL TLYKQHRLD DSRDTRDSS 
      KGTAMSEPQ SIDGESEKL REMAAATES PPKGPFHFN 
      DDQEYPPLT QNSD
    CDS sequence
    • >TC006217 RA
      atgagtaat ataacttca tccgactct aatgggact 
      aagaattat ttcgtgaac tacaaccag gacagcacg 
      tcactagtc ctcggcagt aaaagcggt tatcgtctc 
      tactccctc aacaacgtc gacgacttg gaacttatc 
      tattccaac gatgtcgaa gaaacctac atggtggaa 
      cgtctcttc agcagtagt ctggtggca actgtggct 
      ctctcatcc ccccgaaag ttgaaagtt tgtcatttc 
      aaaaaagga acggaaata tgcaactac tcatactcg 
      aacacgatt ctcgccgtt cgcatgaac cggtcgcgc 
      cttgtcgtc tgtctcgaa gaaagcctc tacatccac 
      aacatccgc gatatgaaa gtcctccac acgatccga 
      gacacgccc cctaatccg caaggcctt tgcgccttg 
      agcataaac cacgacaat tgtcttttg gcgtatcct 
      ggatcggca acgatcggg gaagtacag attttcgat 
      gccgatcat ttgcatgct aaaaccatg attcccgct 
      catgatagt ccgctggcg gctctagcg tttagtcca 
      aatggcaca cgaattgcc acagcttcc gaaaaagga 
      acagtcatt cgtgttttt tcatcagct gatgggcaa 
      aaattgtac gaatttcga cgaggagtg aagcgttgt 
      gttgatatc tcgtctttg gcgttcagc acttgtagt 
      caatttttg tgctgcagt agcaatacg gaaacggtg 
      catgtgttt aagtttgag gaacccaaa gagagtccg 
      aaacgtaat gtggaggag agtagttgg atgggttat 
      ctttcgagt tatttgcca acgcaagtt actgatgtt 
      tttacgcaa gggagggct ttcgcagcg gcgcatttg 
      cccacgcaa gggttgagg aatgttatc tcgattatg 
      acggcccaa aaccagttg cgattgctt gtggcgacc 
      gaagaggca aagttgtac gtttacaac ttggacccg 
      aacgaaggt ggcgacctc acattgtac aaacagcac 
      cgtctcgac gactcacgt gacactcgt gactcatca 
      aaaggtaca gctatgagc gaaccgcaa agcatcgac 
      ggagagtcg gagaagttg agggagatg gcggctgcc 
      accgaatcg ccccccaaa gggcccttc cacttcaac 
      gacgaccag gaatacccg cccttgacg cagaattcg 
      gactga
    mRNA sequence
    • >TC006217 RA
      tgattataa attgtcgat taaaactgg gtccggtca 
      cgtctgtga cagcccaaa tcgttgtca aaatcccca 
      gttttgaat ttggcgcgg ttgcgccgc tgcgcgcga 
      ttccgcact tatcagctg ttcaataca atatggacg 
      tgtagtggt gttggtgtt tgtttggaa aagcaaggt 
      cagcctttg tgtgagaaa taagaagaa agtcgaagt 
      aacaagttg taaatcttt cagatcgcg tcgtagttt 
      tagtgtttt tcgataaaa accgtgtaa tcatcactt 
      cgaactcat gagtaatat aacttcatc cgactctaa 
      tgggactaa gaattattt cgtgaacta caaccagga 
      cagcacgtc actagtcct cggcagtaa aagcggtta 
      tcgtctcta ctccctcaa caacgtcga cgacttgga 
      acttatcta ttccaacga tgtcgaaga aacctacat 
      ggtggaacg tctcttcag cagtagtct ggtggcaac 
      tgtggctct ctcatcccc ccgaaagtt gaaagtttg 
      tcatttcaa aaaaggaac ggaaatatg caactactc 
      atactcgaa cacgattct cgccgttcg catgaaccg 
      gtcgcgcct tgtcgtctg tctcgaaga aagcctcta 
      catccacaa catccgcga tatgaaagt cctccacac 
      gatccgaga cacgccccc taatccgca aggcctttg 
      cgccttgag cataaacca cgacaattg tcttttggc 
      gtatcctgg atcggcaac gatcgggga agtacagat 
      tttcgatgc cgatcattt gcatgctaa aaccatgat 
      tcccgctca tgatagtcc gctggcggc tctagcgtt 
      tagtccaaa tggcacacg aattgccac agcttccga 
      aaaaggaac agtcattcg tgttttttc atcagctga 
      tgggcaaaa attgtacga atttcgacg aggagtgaa 
      gcgttgtgt tgatatctc gtctttggc gttcagcac 
      ttgtagtca atttttgtg ctgcagtag caatacgga 
      aacggtgca tgtgtttaa gtttgagga acccaaaga 
      gagtccgaa acgtaatgt ggaggagag tagttggat 
      gggttatct ttcgagtta tttgccaac gcaagttac 
      tgatgtttt tacgcaagg gagggcttt cgcagcggc 
      gcatttgcc cacgcaagg gttgaggaa tgttatctc 
      gattatgac ggcccaaaa ccagttgcg attgcttgt 
      ggcgaccga agaggcaaa gttgtacgt ttacaactt 
      ggacccgaa cgaaggtgg cgacctcac attgtacaa 
      acagcaccg tctcgacga ctcacgtga cactcgtga 
      ctcatcaaa aggtacagc tatgagcga accgcaaag 
      catcgacgg agagtcgga gaagttgag ggagatggc 
      ggctgccac cgaatcgcc ccccaaagg gcccttcca 
      cttcaacga cgaccagga atacccgcc cttgacgca 
      gaattcgga ctgaggtca cgatacgct caaggacgt 
      cgataggta ccaactcat gttcgggct ggttctgct 
      gattcattt cgtcgtcag gattagcga ggatttgat 
      tttctatct gttcaattt gaacggatg attcatttt 
      atttctaat taacttagt ttttttaaa tttgtattc 
      aagtattgt catttgttg cgggcaaaa attcatatc 
      aattgactc tatttttat ttagtgaat taaaggaca 
      cagttaggt aaaatgtca tggtagtat ttactttga 
      gtgtgctca gagtgaaac tgccggaaa atgtctaat 
      ttattctga aatactgta atgattaag ccacggttt 
      gccccttat tttcgatac atgagggag tgtctgact 
      tgttacagt aggtttttt tccaattat ttgcgcaca 
      ttgtgctgt gtacataca tagttttag gagtgctag 
      tgtgtttat atgaaacga gtaaaatct gtgactaga 
      aaattgtaa agtggacga atgctgtgt gataaataa 
      taatcattt catggataa acttgtttt tattgagtt 
      atgtacgac ggttatttt taaaaattt atcaccgta 
      caatagaac attaaaata ataaaacaa gactcgact 
      c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY