Status
Please wait ...
Gene information
General information
TC006203 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4956594..4977508
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006203
    iBeetle screen iB_01013

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands anterior content darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands potentially content not present - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006203 RA
      MCETPPPVI KSTSAGDLL NNTMTLLPE SPVPQSVEL 
      SKLKRTFTL PRNPFNASK RRPKDSDPE DKKVFRRPS 
      IRKFINKIA QHIGGVPVI NGDSRVPPV GLQTWGPDD 
      TPGVTGLKN HGNTCFINA VLQCISHTD VLAEYFVLD 
      RYKIDLSRR NKLNSKKFG TKGEITEQL ALLLKALWA 
      CQYSPELST SFKQVVERH GSQYKGNQQ HDALEFLQW 
      LLDKVHEDL NTASKKKYK LIKTSGRSD EVIAAETLE 
      NYKRCNNSF IQGVFQAQY RSSLSCSRC RTQSNTFDP 
      FQCISVQLP QFSKQSIYV TVLYTSQQP RQVQIGLSL 
      PSGATVSEL RDILESDTS IERANMLIT EIGETGFLR 
      TFTDTQSVN IISEIDPIY CIEVAQLKD LEEDTTSAY 
      ILLCWINVV QEGDGFKRF GSPYTMQVS RETSYEDLQ 
      KLLLKEMAP ILHDDILTS AQPKGVFKI RISDPACND 
      NEPPCYLEP ELEHPLFME DVDQALALC SEEGGPPHV 
      KLVIEFTQS AKNAIIADD TDIIEEHSS VKQLKAQAL 
      QGGAPLTLE ECLRDYTEA ETLTDAWRC PHCQQYQPV 
      VKTLDMWSL PDILVVHFK RFRQQTLRG CSSAKLINM 
      VDFPVYNLD MSPHLANKG QNISEDQMI MTNGWSPFK 
      RTRKQSNAN DNMYDLYGI CYHHGSDIE TGHYTAACK 
      NPYDNQWYL YDDAKVTNL SKQTDDISA LLVNNSAYI 
      LFYQRRNVG SSTSSAAST SSVGSSTDH WVSRMPKFV 
      PPKNIKIEK KPKVDEETK TSEEVALRN SQNTLDSAA 
      NPRKSLDKS TSTEEVKLY TTSIYINSC GEMDKEVSE 
      GEVKVTMTE PEVRKVYTT TAAVHRHSD NVSRTRLNW 
      MSPQPVRKL NQSQQSV
    CDS sequence
    • >TC006203 RA
      atgtgcgaa actccacct cccgtgata aaatcaaca 
      tcagctggc gacctactc aacaacacg atgacactc 
      ctcccagag tcccccgtt ccccaatcc gtggaactc 
      tccaaactc aagcggacc ttcaccctg ccccggaac 
      cccttcaac gcgtcgaaa cgccgcccc aaagactcc 
      gaccccgag gacaagaaa gtcttcaga cggccgtcc 
      atccgcaag ttcatcaac aaaatcgcg cagcacatc 
      gggggcgtc cccgtcatc aacggggac tcgcgcgtg 
      ccgcccgtg gggctccag acgtggggc cccgacgac 
      acgccgggc gtcacaggg ctcaagaac cacgggaat 
      acgtgcttc atcaacgct gtgctgcaa tgcatctcg 
      catacggac gtcttggcc gagtatttc gttctggat 
      cggtataaa atcgacctc tcgaggcgg aataaactc 
      aattcgaaa aagttcggg acgaagggc gagattacg 
      gaacagttg gcgctgctg ctgaaggcc ctgtgggcc 
      tgtcagtac agccccgag ctgagcacc agcttcaag 
      caagtggtg gaaagacac gggagccaa tacaagggc 
      aaccaacag cacgacgcc ctcgagttc ttgcagtgg 
      ctgctggat aaggtgcac gaggacctc aatacggct 
      tccaagaag aagtataag ctgattaag acttccggc 
      cgttcggac gaggtgatc gccgccgaa accctcgaa 
      aactacaaa cggtgcaac aattccttc atccagggc 
      gtgttccag gcccagtac cggtcatcg ttgagttgt 
      tcccgttgc cgcacccaa tccaacacc ttcgatccg 
      ttccaatgt atctccgtg caattgccc caattcagc 
      aaacaatcg atctatgtc acggtgttg tatacgtcg 
      cagcagcca agacaggtc caaatcggt ttgagttta 
      ccatcggga gctaccgtt tcagaattg agggatatc 
      ctcgaatcg gacacgtcc atcgaacgt gccaatatg 
      ttaattacc gaaattgga gaaacgggg tttttgagg 
      acttttacc gacacgcaa tcggtgaat attataagt 
      gagattgat ccgatctat tgcatcgaa gtagcgcaa 
      ttgaaggat ctggaagag gacacgacc agtgcgtat 
      attttgttg tgttggatt aatgtggtt caagaggga 
      gatggcttt aagaggttc ggcagcccc tacacgatg 
      caagtctcc cgcgaaacc tcctacgag gacctccaa 
      aaactcctc ctcaaagaa atggccccc atcctccac 
      gacgacatt ctcacctca gctcaacca aaaggcgtt 
      ttcaaaata cgcatttcg gatccggct tgtaacgac 
      aacgagcct ccttgttat ctcgaaccc gaactcgag 
      catccactt tttatggaa gatgtcgat caagcgctg 
      gctttatgt agtgaagaa gggggccca ccacatgtc 
      aaactcgtc atcgaattt acacagagt gccaaaaat 
      gcaatcatc gccgacgat acggacatc atcgaggaa 
      cattcgtcc gtgaaacaa ttgaaagcg caagcgcta 
      caagggggc gcacctttg actttggag gagtgttta 
      agggattac accgaagcg gagactttg acggacgcg 
      tggcggtgc ccccattgt cagcagtac caaccggtg 
      gtgaaaaca ctggacatg tggtcgttg ccggacatt 
      ttagtcgtg cattttaag cggtttcga cagcaaact 
      ttgcggggc tgtagcagc gccaagttg atcaatatg 
      gtggatttc ccggtttat aacctcgac atgagcccc 
      catttggcc aataagggc caaaacatc tccgaggac 
      cagatgata atgaccaat ggttggagt ccgtttaaa 
      cgtacccgt aaacaatcg aacgccaac gacaacatg 
      tacgacctt tacggtatt tgctaccat catggtagt 
      gatatcgaa accggtcat tacaccgcc gcctgtaaa 
      aacccctac gacaaccag tggtacctc tacgacgac 
      gcaaaagtc accaacttg tcgaaacaa accgacgac 
      atcagcgcc ctcttggtc aacaacagt gcctacatc 
      ctgttttac caacggcgt aacgttggc tcgagtacc 
      agcagtgcg gccagtacc agttccgtg ggctcctcc 
      accgaccat tgggtgtcc agaatgccc aaatttgta 
      ccaccgaaa aacatcaaa atcgagaaa aagccgaaa 
      gttgacgaa gaaacgaaa acgagcgaa gaagtcgcg 
      ttgcgtaat tcacagaat acgctcgac agtgctgcc 
      aatccacga aaatctcta gacaagtcg acgagtacc 
      gaagaggtt aagttgtat acgacgagt atttatatt 
      aattcttgc ggcgaaatg gacaaggag gtgtcagag 
      ggtgaggtc aaggttacg atgacggag cccgaggtc 
      aggaaagtg tacacaacg acggctgcg gtgcaccgg 
      cactcggat aatgtgtca aggacgagg ctcaattgg 
      atgtcgcca cagccggta cgaaaattg aaccaatcg 
      caacagtcg gtttga
    mRNA sequence
    • >TC006203 RA
      gacatttga caagtgtcg tctggcgcg tcttgtgct 
      aagtgtctt tcgtaatgc cctcggtgc aaagtcacc 
      ctttccgat gatgtgatt atgtgcgaa actccacct 
      cccgtgata aaatcaaca tcagctggc gacctactc 
      aacaacacg atgacactc ctcccagag tcccccgtt 
      ccccaatcc gtggaactc tccaaactc aagcggacc 
      ttcaccctg ccccggaac cccttcaac gcgtcgaaa 
      cgccgcccc aaagactcc gaccccgag gacaagaaa 
      gtcttcaga cggccgtcc atccgcaag ttcatcaac 
      aaaatcgcg cagcacatc gggggcgtc cccgtcatc 
      aacggggac tcgcgcgtg ccgcccgtg gggctccag 
      acgtggggc cccgacgac acgccgggc gtcacaggg 
      ctcaagaac cacgggaat acgtgcttc atcaacgct 
      gtgctgcaa tgcatctcg catacggac gtcttggcc 
      gagtatttc gttctggat cggtataaa atcgacctc 
      tcgaggcgg aataaactc aattcgaaa aagttcggg 
      acgaagggc gagattacg gaacagttg gcgctgctg 
      ctgaaggcc ctgtgggcc tgtcagtac agccccgag 
      ctgagcacc agcttcaag caagtggtg gaaagacac 
      gggagccaa tacaagggc aaccaacag cacgacgcc 
      ctcgagttc ttgcagtgg ctgctggat aaggtgcac 
      gaggacctc aatacggct tccaagaag aagtataag 
      ctgattaag acttccggc cgttcggac gaggtgatc 
      gccgccgaa accctcgaa aactacaaa cggtgcaac 
      aattccttc atccagggc gtgttccag gcccagtac 
      cggtcatcg ttgagttgt tcccgttgc cgcacccaa 
      tccaacacc ttcgatccg ttccaatgt atctccgtg 
      caattgccc caattcagc aaacaatcg atctatgtc 
      acggtgttg tatacgtcg cagcagcca agacaggtc 
      caaatcggt ttgagttta ccatcggga gctaccgtt 
      tcagaattg agggatatc ctcgaatcg gacacgtcc 
      atcgaacgt gccaatatg ttaattacc gaaattgga 
      gaaacgggg tttttgagg acttttacc gacacgcaa 
      tcggtgaat attataagt gagattgat ccgatctat 
      tgcatcgaa gtagcgcaa ttgaaggat ctggaagag 
      gacacgacc agtgcgtat attttgttg tgttggatt 
      aatgtggtt caagaggga gatggcttt aagaggttc 
      ggcagcccc tacacgatg caagtctcc cgcgaaacc 
      tcctacgag gacctccaa aaactcctc ctcaaagaa 
      atggccccc atcctccac gacgacatt ctcacctca 
      gctcaacca aaaggcgtt ttcaaaata cgcatttcg 
      gatccggct tgtaacgac aacgagcct ccttgttat 
      ctcgaaccc gaactcgag catccactt tttatggaa 
      gatgtcgat caagcgctg gctttatgt agtgaagaa 
      gggggccca ccacatgtc aaactcgtc atcgaattt 
      acacagagt gccaaaaat gcaatcatc gccgacgat 
      acggacatc atcgaggaa cattcgtcc gtgaaacaa 
      ttgaaagcg caagcgcta caagggggc gcacctttg 
      actttggag gagtgttta agggattac accgaagcg 
      gagactttg acggacgcg tggcggtgc ccccattgt 
      cagcagtac caaccggtg gtgaaaaca ctggacatg 
      tggtcgttg ccggacatt ttagtcgtg cattttaag 
      cggtttcga cagcaaact ttgcggggc tgtagcagc 
      gccaagttg atcaatatg gtggatttc ccggtttat 
      aacctcgac atgagcccc catttggcc aataagggc 
      caaaacatc tccgaggac cagatgata atgaccaat 
      ggttggagt ccgtttaaa cgtacccgt aaacaatcg 
      aacgccaac gacaacatg tacgacctt tacggtatt 
      tgctaccat catggtagt gatatcgaa accggtcat 
      tacaccgcc gcctgtaaa aacccctac gacaaccag 
      tggtacctc tacgacgac gcaaaagtc accaacttg 
      tcgaaacaa accgacgac atcagcgcc ctcttggtc 
      aacaacagt gcctacatc ctgttttac caacggcgt 
      aacgttggc tcgagtacc agcagtgcg gccagtacc 
      agttccgtg ggctcctcc accgaccat tgggtgtcc 
      agaatgccc aaatttgta ccaccgaaa aacatcaaa 
      atcgagaaa aagccgaaa gttgacgaa gaaacgaaa 
      acgagcgaa gaagtcgcg ttgcgtaat tcacagaat 
      acgctcgac agtgctgcc aatccacga aaatctcta 
      gacaagtcg acgagtacc gaagaggtt aagttgtat 
      acgacgagt atttatatt aattcttgc ggcgaaatg 
      gacaaggag gtgtcagag ggtgaggtc aaggttacg 
      atgacggag cccgaggtc aggaaagtg tacacaacg 
      acggctgcg gtgcaccgg cactcggat aatgtgtca 
      aggacgagg ctcaattgg atgtcgcca cagccggta 
      cgaaaattg aaccaatcg caacagtcg gtttgaata 
      cttgtaaat aagcactaa gtagttgcc tttgttaag 
      tgggtttta atcagatat ttaagagat ttgttttat 
      tttttaata ttttattta ccgtttaag taaatattt 
      attttttgt gagggtatt tataccgtt tttgttagt 
      ggtgatatt aactgagct tgtgtaatc ttgcaatta 
      atcaccgac aataagttt tcagttctt ttgatggct 
      ttattttgt tttttgtta aattactag tcatcattg 
      aaataccaa ataaaagta tttccactt attatagga 
      ttaaaatag tagcagtcg gtactgtaa cgtgtaacg 
      tgccttaaa ttggaataa acattatta cccttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY