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Gene information
General information
TC006180 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4575310..4579588
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006180
    iBeetle screen iB_01007

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006180 RA
      MSHLTEHLI IGTVLSTLF IIFNYSLHR IEEGHVGVY 
      FRGGALLPV TSSPGYHMM IPLLTIYKS VQVTLQTDE 
      VKNVPCGTS GGVMIYFDR IEVVNHLNA NSVMDIVRN 
      YTADYDKTL IFNKIHHEL NQFCSIHTL HEVYIDLFD 
      QIDENLKQA LQRDLLEMA PGLTIQAVR VTKPKIPEV 
      IRKNYELME GEKTKLLIA TQHQKVVEK DAETERKRA 
      VIEAEKEAQ VAKIQYQQK IMEKESLQR IAQIEDEMH 
      LARQKSHAD AEFYRMKQQ AEVNKLLYT PEYIELKKY 
      ESLSQNNKV YFGKDIPQT FISSGCSGE TFADKKKLE 
      GRP
    CDS sequence
    • >TC006180 RA
      atgtcacac ttgactgaa catttaata attggaact 
      gtgttatca actcttttt atcattttt aattattcc 
      ttgcatcgg atagaagag ggacatgtt ggggtgtat 
      tttagggga ggcgcactt ttgcctgta acaagctca 
      ccagggtat catatgatg atacccttg ctgacaata 
      tacaaatcg gttcaggtt actcttcag actgacgaa 
      gtcaagaat gtcccttgc ggcaccagc ggcggtgtt 
      atgatttat ttcgataga atagaagtt gttaatcat 
      ttaaatgcc aatagcgtt atggatatt gtccgaaac 
      tacactgcc gactacgac aagacccta atcttcaac 
      aaaatccac cacgagttg aatcaattt tgcagtatt 
      cacacccta cacgaggtt tacatcgat ttgttcgac 
      caaatcgac gagaacctc aaacaagcc ctccagcgc 
      gatttgctt gaaatggcc cctggcctc accatccaa 
      gccgtccgc gtaacaaaa ccaaagatc cccgaagtc 
      attcgcaaa aactacgaa ttgatggaa ggcgaaaag 
      accaaattg ttgatcgcg acgcaacat caaaaagtc 
      gttgaaaaa gatgccgaa actgaacga aaacgagct 
      gtgattgaa gctgaaaaa gaagcgcaa gttgcaaaa 
      atccaatat cagcaaaaa attatggag aaagagtca 
      ttgcaacga attgctcaa atcgaagac gaaatgcat 
      ttagcaagg cagaaaagt cacgcagat gctgaattc 
      taccgaatg aaacaacaa gctgaagtt aataaattg 
      ttgtacacg ccggaatac atcgaactg aaaaagtac 
      gagtccttg tcgcaaaac aacaaagtt tacttcggc 
      aaagacatc ccgcaaact tttatcagc tcggggtgt 
      tccggcgaa actttcgcc gataagaag aagttagaa 
      gggcgaccc tga
    mRNA sequence
    • >TC006180 RA
      aggaaaaaa ttttgaact ttttgttat cttgtgcct 
      atttcttta aaataaaat aggccccaa ccacgcgtg 
      actttgaac gtaagaaac tccaattct gcgacagtg 
      aagcaaaaa gtggatgtg gattcattt tgtaaaaat 
      tcaattttt tgtgaaaaa atttgattt tttgttatt 
      tattttcta gtgttcact caagtatgt gttcagaat 
      caatattgt gtttgtgct tggtggagt gtagttttc 
      ccggttgtt ctttctcaa cttacataa cctcaaaaa 
      tgtcccaag tagaaatta aagatttcc gataagtgt 
      tattagatg atgtgttca tattaatat ttaaccagt 
      cgattgctt tatttgcat aaaaatgtc acacttgac 
      tgaacattt aataattgg aactgtgtt atcaactct 
      ttttatcat ttttaatta ttccttgca tcggataga 
      agagggaca tgttggggt gtattttag gggaggcgc 
      acttttgcc tgtaacaag ctcaccagg gtatcatat 
      gatgatacc cttgctgac aatatacaa atcggttca 
      ggttactct tcagactga cgaagtcaa gaatgtccc 
      ttgcggcac cagcggcgg tgttatgat ttatttcga 
      tagaataga agttgttaa tcatttaaa tgccaatag 
      cgttatgga tattgtccg aaactacac tgccgacta 
      cgacaagac cctaatctt caacaaaat ccaccacga 
      gttgaatca attttgcag tattcacac cctacacga 
      ggtttacat cgatttgtt cgaccaaat cgacgagaa 
      cctcaaaca agccctcca gcgcgattt gcttgaaat 
      ggcccctgg cctcaccat ccaagccgt ccgcgtaac 
      aaaaccaaa gatccccga agtcattcg caaaaacta 
      cgaattgat ggaaggcga aaagaccaa attgttgat 
      cgcgacgca acatcaaaa agtcgttga aaaagatgc 
      cgaaactga acgaaaacg agctgtgat tgaagctga 
      aaaagaagc gcaagttgc aaaaatcca atatcagca 
      aaaaattat ggagaaaga gtcattgca acgaattgc 
      tcaaatcga agacgaaat gcatttagc aaggcagaa 
      aagtcacgc agatgctga attctaccg aatgaaaca 
      acaagctga agttaataa attgttgta cacgccgga 
      atacatcga actgaaaaa gtacgagtc cttgtcgca 
      aaacaacaa agtttactt cggcaaaga catcccgca 
      aacttttat cagctcggg gtgttccgg cgaaacttt 
      cgccgataa gaagaagtt agaagggcg accctgatt 
      gtgatttaa ttctaagtt accgaaata aagttttgt 
      cgatttc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY