Status
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Gene information
General information
TC006152 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 3878874..3887328
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006152
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_01003

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Fertility
    vitellogenic egg chamber number increased - Number of animals showing the phenotype: 4

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 83.33% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    Pupal morphology
    wings slightly shortened - Number of animals showing the phenotype: 8
    Adult morphology
    wings not closed - Number of animals showing the phenotype: 5


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006152 RA
      MPLPKRVIE PVHVARGTL PEDFPLVSE LEAVTNGTL 
      ANTVRQLSS LSKHAEDLF GELARDAQQ LYDRSNSLQ 
      ARIDRLAIK VTQLDSTVE EVSLQDIHM RKAFKSTVA 
      FDQQIFSRA TMPTAMLET YHSCDKPPP LHKLNCYRD 
      DGKDGLKFY TDPNYFFEL WRQEMLNDT ERMMHDRGK 
      KPHRPRTEG GQGNRHKKR VRQPHNTRE RQKQIAVGH 
      GEYIMPQQN TIYRTPQPI IQQQQQMLP TEEMIEPRP 
      PRPNSIEIR RSYQQETTD GLHSPTYPP YEEYGQGPL 
      SSESLYQGG TPTRGGKVR PSQPPPAPP SNTSTPSSN 
      TPTRGRSLS GRDNLPPPP PPPDTTMSP PNGIPAHLA 
      TQSRSNSPH QATPPEPVQ DLPPPPPIP KQASPPKQQ 
      TPQAPPPPP PPPMPLLNG PTTSPPLVN GDLAKIINS 
      ASPPQLTPV KDRPKQAPP IVDPRNDLL KAIRDGIKL 
      RKVEKIEQK EVERGNGFH DVASILARR VAVEFSDSD 
      SGSDSECDS EGWGENETS A
    CDS sequence
    • >TC006152 RA
      atgcctctg ccaaagagg gtgatcgaa ccggtccac 
      gtggccaga ggcacactt ccagaggat tttcctcta 
      gtgtccgaa ttggaagcc gtcaccaac ggaacttta 
      gcgaatacc gtacgacaa ctttcctca ctctcaaaa 
      catgccgaa gatttattc ggagaattg gccagagat 
      gcccaacaa ctctacgac agatcgaat tcactgcaa 
      gcgagaatc gacagactc gctatcaaa gtcacacaa 
      ttggacagc accgtcgag gaggtctct ttgcaggat 
      atccacatg aggaaggcg ttcaagagc acggtggct 
      ttcgatcaa caaatattc tcccgagcg actatgcca 
      acggccatg ttggaaacg tatcattcc tgtgacaaa 
      ccgccccca ctccacaaa ctcaattgt taccgtgac 
      gacggcaaa gacggtctg aaattctac accgatccg 
      aactacttc ttcgaatta tggcgtcaa gaaatgctc 
      aacgacacc gaacgtatg atgcacgac cgtggcaag 
      aaaccacac cgaccacga accgaaggc ggccaagga 
      aaccgccac aaaaaacgc gtccgtcaa ccacacaac 
      acccgcgaa agacaaaag caaatcgcc gtaggccac 
      ggcgaatac atcatgcca caacaaaac accatctat 
      cgcacccca caacccatc atccagcaa caacaacaa 
      atgttacca accgaagaa atgattgaa ccacgccca 
      ccacgaccg aactcaatc gaaatacga cgctcgtac 
      caacaggaa accaccgac ggtctccat tcccccact 
      taccccccg tacgaggag tatggccaa ggcccactc 
      agttccgaa tcattatac caaggaggc acgcccaca 
      cgaggcggt aaagtccgc ccaagccag cccccaccc 
      gcaccacct agcaacacg agcacgccc tcgtccaat 
      accccgacg cgagggcgg agcctctcc ggccgcgac 
      aacctacca ccaccgccc ccaccaccc gacacgacc 
      atgtcaccg cccaacggc atccccgca catctcgcc 
      acccaatcg cgttcaaac agtccgcat caagccaca 
      cctcccgaa ccggtgcaa gacttacct ccaccaccg 
      cccatacct aagcaagcc tcgcccccc aaacaacaa 
      actccacaa gccccgcct ccgccacca cctccacca 
      atgccgtta ctgaacggc ccgacgact agtccccca 
      ttggttaac ggcgatttg gcgaaaatt ataaatagt 
      gcgtcgccg ccgcaattg acacctgtc aaagatcgg 
      ccaaagcag gcgccgcct atcgtcgat cccaggaat 
      gatttactc aaagctatc cgagatggg attaagttg 
      cgcaaggtg gagaagatt gaacagaag gaggtggag 
      agagggaac ggtttccac gatgttgcg agtatttta 
      gctcggagg gttgcagtc gagtttagc gattcggat 
      tcaggaagc gattcggag tgtgatagt gagggttgg 
      ggcgaaaat gaaacgtcg gcgtga
    mRNA sequence
    • >TC006152 RA
      gtcctgctc aatcgtgtt tgttgggtg taccgcttc 
      gccattttc tgcgatatt tattaaatt atcgccgtt 
      tttaacgaa acaattagt gtcgtgtgt gcaaaaaca 
      tgtgtggca acgtttgcg ccccccagt gagtgtccc 
      agaccatgc cgagtgaac actaaacaa ccatgcctc 
      tgccaaaga gggtgatcg aaccggtcc acgtggcca 
      gaggcacac ttccagagg attttcctc tagtgtccg 
      aattggaag ccgtcacca acggaactt tagcgaata 
      ccgtacgac aactttcct cactctcaa aacatgccg 
      aagatttat tcggagaat tggccagag atgcccaac 
      aactctacg acagatcga attcactgc aagcgagaa 
      tcgacagac tcgctatca aagtcacac aattggaca 
      gcaccgtcg aggaggtct ctttgcagg atatccaca 
      tgaggaagg cgttcaaga gcacggtgg ctttcgatc 
      aacaaatat tctcccgag cgactatgc caacggcca 
      tgttggaaa cgtatcatt cctgtgaca aaccgcccc 
      cactccaca aactcaatt gttaccgtg acgacggca 
      aagacggtc tgaaattct acaccgatc cgaactact 
      tcttcgaat tatggcgtc aagaaatgc tcaacgaca 
      ccgaacgta tgatgcacg accgtggca agaaaccac 
      accgaccac gaaccgaag gcggccaag gaaaccgcc 
      acaaaaaac gcgtccgtc aaccacaca acacccgcg 
      aaagacaaa agcaaatcg ccgtaggcc acggcgaat 
      acatcatgc cacaacaaa acaccatct atcgcaccc 
      cacaaccca tcatccagc aacaacaac aaatgttac 
      caaccgaag aaatgattg aaccacgcc caccacgac 
      cgaactcaa tcgaaatac gacgctcgt accaacagg 
      aaaccaccg acggtctcc attccccca cttaccccc 
      cgtacgagg agtatggcc aaggcccac tcagttccg 
      aatcattat accaaggag gcacgccca cacgaggcg 
      gtaaagtcc gcccaagcc agcccccac ccgcaccac 
      ctagcaaca cgagcacgc cctcgtcca ataccccga 
      cgcgagggc ggagcctct ccggccgcg acaacctac 
      caccaccgc ccccaccac ccgacacga ccatgtcac 
      cgcccaacg gcatccccg cacatctcg ccacccaat 
      cgcgttcaa acagtccgc atcaagcca cacctcccg 
      aaccggtgc aagacttac ctccaccac cgcccatac 
      ctaagcaag cctcgcccc ccaaacaac aaactccac 
      aagccccgc ctccgccac cacctccac caatgccgt 
      tactgaacg gcccgacga ctagtcccc cattggtta 
      acggcgatt tggcgaaaa ttataaata gtgcgtcgc 
      cgccgcaat tgacacctg tcaaagatc ggccaaagc 
      aggcgccgc ctatcgtcg atcccagga atgatttac 
      tcaaagcta tccgagatg ggattaagt tgcgcaagg 
      tggagaaga ttgaacaga aggaggtgg agagaggga 
      acggtttcc acgatgttg cgagtattt tagctcgga 
      gggttgcag tcgagttta gcgattcgg attcaggaa 
      gcgattcgg agtgtgata gtgagggtt ggggcgaaa 
      atgaaacgt cggcgtgac atagaagga aggctagca 
      cggctagtc aagtgtatt gaagcaacg aattttcaa 
      tttttaacg agcaaactt tagcaatat gccaaattt 
      ccagtatta ttgaggcat tgcatgata agcttatta 
      gattgcgtt tacagtgga tggttttga cttgggttg 
      agatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY