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Gene information
General information
TC006147 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 3807895..3811807
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006147
    iBeetle screen iB_01002

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 10 (16 dpi)
    Days post injection


    pupal molt not fulfilled (died as L7 or prepupa) - Number of animals showing the phenotype: 10 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006147 RA
      MDSDASDNY EDLYSEEDA SKHDKLVQK VLSLNKTQQ 
      LKKPSRTEP TLQISEFNL VKSITGHKG AVHLNDLAK 
      TLKSRKSRL KISENVKKI QRHSRTLPK PLKKPQANR 
      IRRSVAYEK TRLELDRWE ATVTANRVA TNLSFPLDN 
      DYKLEKKEN KNFTSMWRV KSALQQELE KIEPTVEEY 
      HIDLEKEKF PLTMKEMLV RRQEAAKLR AYQGYREAK 
      ARRQRKIKS KKYHRIQKR EKIKKQLKE FELLQKTNP 
      EEALKKLEE IEKTRIEER FSLRHKSTG KWARNQQVR 
      AKYDKESRQ VLAQQLTLS RELTQKLKN NNSDSDEDE 
      AKEEEKVPE VIDKDNPWV NGIKPGKDV ADFISGYRK 
      YWMEKNKEG ETGQLQEDA QENVTVSEE IQMENNKQL 
      EEEASTIDE GYRVEKNKD EEEKTEQSQ EDSPEATLT 
      ISEEKVTKK KKQSKVGKK KNSTEDLKQ SKSTSDWEI 
      SVVDNATDV EDMFNKLEK KLQRTMLKR VKKLKHKIN 
      KEDKTKTEP KRKKVDNKV DLTLNPQKK IPIIDEELI 
      ENTNSDAEN SQNNETLSN LKNISTKEV AQNESNIDP 
      DKYMKVKPT NLSTAIPDI LTEDNEEPT QREIISEAF 
      EDDDIVQEF AKEKQDEVD RDKPKDIDL SLPGWGSWG 
      GTNIKVNKR KKRRFTIKA PPKVPRRDD NKGTLIINE 
      KAQNKIKEH MVSEVPFPF KTVKDFEAS MRAPISNTF 
      VPETAYRRL IKPAVTTKM GTIIEPISK DILINKRN
    CDS sequence
    • >TC006147 RA
      atggacagt gacgcgagt gataactat gaggatttg 
      tacagcgag gaagacgcc tcaaagcac gacaaattg 
      gtgcagaag gtgctaagt ttaaataag acgcaacag 
      ctcaagaag ccctcccgg accgaaccg accctccaa 
      atctccgaa ttcaatttg gtgaagtcc ataaccggc 
      cataaaggt gcagtccac ttaaatgac ttggccaaa 
      acgttaaaa tcgcgaaaa agtcggctc aaaatcagc 
      gaaaacgtc aaaaaaatc cagagacac tcgcgcacc 
      ctccccaag cctttgaag aagccccaa gccaaccgt 
      atccggcga agtgttgcg tatgagaag acgcgcctc 
      gaactggac cggtgggag gccacagtc actgcaaat 
      cgcgtcgcg actaatttg tccttcccc ttagacaat 
      gattataaa ttggagaaa aaagaaaat aagaatttt 
      actagcatg tggcgcgtg aaatcggcc cttcaacag 
      gaattggag aagattgag cccacagtt gaggagtat 
      catattgat ttagagaag gagaagttt ccactaaca 
      atgaaggaa atgttggtt cgacggcaa gaagcggca 
      aagttgagg gcgtatcaa gggtataga gaagccaaa 
      gcgcgtcga cagaggaaa atcaagagc aagaagtat 
      cacaggata cagaaacgt gaaaaaatt aagaaacag 
      ttgaaggag tttgagctg ttgcaaaaa acgaatcca 
      gaggaggcg ctcaaaaaa ttggaggag attgagaag 
      acgaggatt gaggagagg tttagtttg aggcataaa 
      agtacgggg aagtgggcg aggaatcag caagtcaga 
      gccaaatat gacaaagag agtcggcaa gttttagca 
      caacaatta acattaagt cgcgagctt acgcaaaaa 
      ttaaagaat aataatagc gactctgat gaagatgaa 
      gcaaaagaa gaagaaaaa gttcctgaa gtgattgat 
      aaagataat ccatgggtg aacggaatc aagccagga 
      aaagatgtt gccgatttt atcagcggt tatcggaaa 
      tattggatg gagaaaaat aaagaagga gaaactggg 
      caattacaa gaagacgct caagaaaat gtgactgtt 
      agtgaagaa atccagatg gagaataat aaacaattg 
      gaagaagaa gcttcgact attgacgaa ggatatcgg 
      gtggagaag aataaagat gaagaagaa aaaacagaa 
      caatctcaa gaagacagt cctgaagca actttgact 
      attagtgaa gaaaaagta acaaaaaag aaaaaacaa 
      tcaaaggtt gggaagaaa aagaattca actgaagat 
      ttaaaacaa tccaaatca acatctgac tgggaaatt 
      agtgttgtt gataatgct acagacgtc gaagacatg 
      tttaataaa ttagaaaaa aagcttcaa agaacaatg 
      ttgaaaaga gtgaagaaa ttaaaacat aaaataaat 
      aaagaagac aaaacgaag acagaacct aaaaggaaa 
      aaagttgat aacaaagtc gatttaact ctaaatccc 
      caaaagaaa attccaata attgacgaa gaattgatc 
      gaaaacacg aattccgat gccgaaaac tcccaaaat 
      aacgaaacc ttgtcaaac ctaaaaaac atctcaact 
      aaagaagtg gcccaaaac gagtcaaac atagacccc 
      gacaaatac atgaaagtc aaaccaaca aatctctca 
      actgcaatt cccgacatt ttaacagaa gataacgaa 
      gaaccaact caacgcgaa atcatttct gaagcattt 
      gaagacgac gatatcgtc caagaattc gcaaaagaa 
      aaacaggac gaagtcgac cgagacaag ccgaaagat 
      atcgatctg agtttgcca ggttggggc agttggggt 
      ggtaccaac ataaaagta aacaagcgc aaaaagcga 
      agattcaca atcaaagcg ccgccaaaa gtccctaga 
      agggacgat aataaagga accctgata attaatgag 
      aaagcgcaa aataaaatt aaagaacac atggtgtct 
      gaagttccg ttccctttc aaaactgtg aaggatttt 
      gaggcgagc atgagggca cctattagt aacactttt 
      gtaccggaa acggcctac aggaggttg atcaaacca 
      gcggttacg accaagatg ggtacaatc atcgaacct 
      ataagcaaa gatatactt ataaataaa cgtaattga
    mRNA sequence
    • >TC006147 RA
      gtgcaacac ttaagttgg tcgattgtg attttcacg 
      tcacaaacg tcaaatcga cgaacataa cctccaaat 
      acacgtgta ttaatttgt ttaaataat tcaataaaa 
      tggacagtg acgcgagtg ataactatg aggatttgt 
      acagcgagg aagacgcct caaagcacg acaaattgg 
      tgcagaagg tgctaagtt taaataaga cgcaacagc 
      tcaagaagc cctcccgga ccgaaccga ccctccaaa 
      tctccgaat tcaatttgg tgaagtcca taaccggcc 
      ataaaggtg cagtccact taaatgact tggccaaaa 
      cgttaaaat cgcgaaaaa gtcggctca aaatcagcg 
      aaaacgtca aaaaaatcc agagacact cgcgcaccc 
      tccccaagc ctttgaaga agccccaag ccaaccgta 
      tccggcgaa gtgttgcgt atgagaaga cgcgcctcg 
      aactggacc ggtgggagg ccacagtca ctgcaaatc 
      gcgtcgcga ctaatttgt ccttcccct tagacaatg 
      attataaat tggagaaaa aagaaaata agaatttta 
      ctagcatgt ggcgcgtga aatcggccc ttcaacagg 
      aattggaga agattgagc ccacagttg aggagtatc 
      atattgatt tagagaagg agaagtttc cactaacaa 
      tgaaggaaa tgttggttc gacggcaag aagcggcaa 
      agttgaggg cgtatcaag ggtatagag aagccaaag 
      cgcgtcgac agaggaaaa tcaagagca agaagtatc 
      acaggatac agaaacgtg aaaaaatta agaaacagt 
      tgaaggagt ttgagctgt tgcaaaaaa cgaatccag 
      aggaggcgc tcaaaaaat tggaggaga ttgagaaga 
      cgaggattg aggagaggt ttagtttga ggcataaaa 
      gtacgggga agtgggcga ggaatcagc aagtcagag 
      ccaaatatg acaaagaga gtcggcaag ttttagcac 
      aacaattaa cattaagtc gcgagctta cgcaaaaat 
      taaagaata ataatagcg actctgatg aagatgaag 
      caaaagaag aagaaaaag ttcctgaag tgattgata 
      aagataatc catgggtga acggaatca agccaggaa 
      aagatgttg ccgatttta tcagcggtt atcggaaat 
      attggatgg agaaaaata aagaaggag aaactgggc 
      aattacaag aagacgctc aagaaaatg tgactgtta 
      gtgaagaaa tccagatgg agaataata aacaattgg 
      aagaagaag cttcgacta ttgacgaag gatatcggg 
      tggagaaga ataaagatg aagaagaaa aaacagaac 
      aatctcaag aagacagtc ctgaagcaa ctttgacta 
      ttagtgaag aaaaagtaa caaaaaaga aaaaacaat 
      caaaggttg ggaagaaaa agaattcaa ctgaagatt 
      taaaacaat ccaaatcaa catctgact gggaaatta 
      gtgttgttg ataatgcta cagacgtcg aagacatgt 
      ttaataaat tagaaaaaa agcttcaaa gaacaatgt 
      tgaaaagag tgaagaaat taaaacata aaataaata 
      aagaagaca aaacgaaga cagaaccta aaaggaaaa 
      aagttgata acaaagtcg atttaactc taaatcccc 
      aaaagaaaa ttccaataa ttgacgaag aattgatcg 
      aaaacacga attccgatg ccgaaaact cccaaaata 
      acgaaacct tgtcaaacc taaaaaaca tctcaacta 
      aagaagtgg cccaaaacg agtcaaaca tagaccccg 
      acaaataca tgaaagtca aaccaacaa atctctcaa 
      ctgcaattc ccgacattt taacagaag ataacgaag 
      aaccaactc aacgcgaaa tcatttctg aagcatttg 
      aagacgacg atatcgtcc aagaattcg caaaagaaa 
      aacaggacg aagtcgacc gagacaagc cgaaagata 
      tcgatctga gtttgccag gttggggca gttggggtg 
      gtaccaaca taaaagtaa acaagcgca aaaagcgaa 
      gattcacaa tcaaagcgc cgccaaaag tccctagaa 
      gggacgata ataaaggaa ccctgataa ttaatgaga 
      aagcgcaaa ataaaatta aagaacaca tggtgtctg 
      aagttccgt tccctttca aaactgtga aggattttg 
      aggcgagca tgagggcac ctattagta acacttttg 
      taccggaaa cggcctaca ggaggttga tcaaaccag 
      cggttacga ccaagatgg gtacaatca tcgaaccta 
      taagcaaag atatactta taaataaac gtaattgaa 
      ctcatttcg ttgttttat tcgttttct gacccttta 
      gagtttagt tttttttga aattatatc atattgatg 
      tctagaaat taaaaacta aaaaaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY