| General information | |||
| TC006139 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 3431645..3443090 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC006139 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_01001 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 5 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 80.0% (includes death as larva, prepupa, pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (16 dpi)
- >TC006139 RA
MYGACLCIL ASVKILLLA PLFIVGLKA QYSSQPYDR NRTDNQYDP YNSQYDRQN PPYDRQNPP YDRQNPPYD RQNPPYDRR NPPYGQPPY DRNDRYNSP PYDRNQPYN QSQYGRQNQ SQYGPSYDN RDPYNRDRL DNRDQYYVG NRDQYNRDR DQYGSNRDR DQYGGNRDQ YGGNRDQYG GNRDQYGGN RDQYGGNRD QYGGNRDQY GGNRDQYGG NRDQYGNRD QYGGNRDQY DRDRDRYDS DGDRDRYGS PLFETNSDC CAEYCYAKD QNQYVQFAT KTAYQIAFS KSTNQQHRV PDCTPIQFW SINRHGTRY PSARTIERL RQLYKIQRE IVRNYQERN SYPNNGRLC PEDLDLIKG WRWNETVNE RNANALTYQ GVTDMKFLA RRYASKFDE LLREPYNEM TYSFQYTDT DRTHDSYQA YIEGLFKEK AYQVHANVF NNDRLIKPT RSCNAWLRE VDQNPQTFN EYMKFKHNR EYQQMVRDV FRRLGFRYT LNDTVLSDM YDMCRFEKA WNLDAMSPW CIVFNKEQL KLLEYAEDL KYYYKSGYG NEVNRQIGC PPVKDLYEK FERTVNNGG TPTGNKVTV FFTHSVTIQ TFLTAMGIA KDHQPLTAE NYYQQQHRK WRTSKIDPF ASNLAAVLY QCRSGERYR VMFFLNEEP VNYPECSVG LCNWSTVQN KLRGVVDNC NLDFCDRNA ATAVQYNYI IYFSSLIAM FLSVKI
- >TC006139 RA
atgtatggt gcatgctta tgcatactg gcctcagtc aagatactc ttgctggcg ccacttttc atagttggt ctcaaagct cagtacagc tcacaaccc tacgatcga aatcgtacc gacaatcag tacgatcct tataactca caatatgac cgtcaaaac ccgccatac gatcgacaa aatccgcca tacgaccgc caaaacccg ccatacgac cgccaaaac cctccatat gaccgacgc aacccacca tatggacaa cctccatat gaccgcaat gatcgctac aattcgccc ccatatgac cggaaccag ccatacaac cagtcgcag tatggccgc caaaatcaa agccagtat ggaccttca tacgacaat cgcgatcca tacaatcgt gatcgtttg gataaccgt gatcagtac tatgtagga aatcgtgat cagtataat cgtgatcgt gatcaatac ggaagtaat cgtgatcgc gatcagtat ggaggaaat cgtgatcag tatggaggg aaccgtgat caatatgga ggaaaccgt gatcaatat ggaggcaat cgtgatcag tatggagga aatcgcgat cagtatgga ggcaatagg gatcaatat gggggcaat cgggatcag tatggagga aacagggat cagtatgga aatcgagac cagtatgga ggaaatcgg gatcagtat gaccgcgat cgtgatcga tatgatagt gatggtgat cgtgatcgt tacggttcg cccttgttt gaaacaaac tctgattgt tgtgcggaa tattgctat gcgaaagat caaaatcaa tatgttcag tttgcgacc aagacggcc tatcagatt gcgttttct aagtcgaca aatcagcag cacagagta ccagactgt acaccaatt caattttgg agcatcaat agacacggt acgagatat cctagtgct agaaccatc gaacggttg agacaactg tataaaatt caacgcgaa attgtgcgc aactatcag gaaagaaat tcgtatccc aacaacgga cgactctgt cctgaagat ttagatctc atcaaaggc tggcgctgg aatgaaact gtcaacgaa cgcaatgcc aacgccttg acttaccag ggagtaaca gatatgaaa tttttggcg cgccgttac gcttcaaag tttgacgag ttgttgcga gaaccctat aacgaaatg acctacagt tttcaatat actgacact gatcggact cacgatagc taccaggct tacattgaa gggcttttc aaggaaaaa gcttatcaa gttcatgct aatgttttt aataatgac agactgatc aagcctaca cgatcatgc aacgcctgg ctgagagaa gtggaccaa aatccgcaa acattcaat gaatacatg aaatttaaa cacaaccga gagtatcag caaatggtc agagacgtg ttccgaaga ctaggtttc agatacaca ctgaatgac actgtttta tccgatatg tacgacatg tgccgcttt gaaaaagct tggaatttg gatgctatg tctccttgg tgcatagtt tttaacaaa gaacaacta aaacttcta gaatacgcc gaagatttg aaatactat tacaaaagc ggttacggc aatgaagtt aaccgacaa attggttgt ccacctgtg aaagatctt tacgaaaaa ttcgaacgt acagtcaat aacggggga acccccact ggaaacaag gttacagtt ttcttcact cattctgtg acgattcag acgttttta acggcgatg ggtattgca aaggaccat caaccatta actgcggaa aattactac cagcagcaa caccgcaag tggcgtact tcgaaaatt gatccgttt gcgtcaaat ttggctgca gttttatat caatgccgt agcggagaa cgttatcgc gtcatgttc ttcttgaac gaggaacca gtgaattat ccagaatgt tcagtcggt ctttgcaac tggagtacc gtccaaaat aaattgcga ggtgttgta gacaattgt aatttagat ttctgcgat cgtaacgct gcaactgcc gtccaatac aattacatc atttatttc tcaagtctc atcgccatg tttctaagt gttaaaatc taa
- >TC006139 RA
gcggtagag aaaataagt atgtatggt gcatgctta tgcatactg gcctcagtc aagatactc ttgctggcg ccacttttc atagttggt ctcaaagct cagtacagc tcacaaccc tacgatcga aatcgtacc gacaatcag tacgatcct tataactca caatatgac cgtcaaaac ccgccatac gatcgacaa aatccgcca tacgaccgc caaaacccg ccatacgac cgccaaaac cctccatat gaccgacgc aacccacca tatggacaa cctccatat gaccgcaat gatcgctac aattcgccc ccatatgac cggaaccag ccatacaac cagtcgcag tatggccgc caaaatcaa agccagtat ggaccttca tacgacaat cgcgatcca tacaatcgt gatcgtttg gataaccgt gatcagtac tatgtagga aatcgtgat cagtataat cgtgatcgt gatcaatac ggaagtaat cgtgatcgc gatcagtat ggaggaaat cgtgatcag tatggaggg aaccgtgat caatatgga ggaaaccgt gatcaatat ggaggcaat cgtgatcag tatggagga aatcgcgat cagtatgga ggcaatagg gatcaatat gggggcaat cgggatcag tatggagga aacagggat cagtatgga aatcgagac cagtatgga ggaaatcgg gatcagtat gaccgcgat cgtgatcga tatgatagt gatggtgat cgtgatcgt tacggttcg cccttgttt gaaacaaac tctgattgt tgtgcggaa tattgctat gcgaaagat caaaatcaa tatgttcag tttgcgacc aagacggcc tatcagatt gcgttttct aagtcgaca aatcagcag cacagagta ccagactgt acaccaatt caattttgg agcatcaat agacacggt acgagatat cctagtgct agaaccatc gaacggttg agacaactg tataaaatt caacgcgaa attgtgcgc aactatcag gaaagaaat tcgtatccc aacaacgga cgactctgt cctgaagat ttagatctc atcaaaggc tggcgctgg aatgaaact gtcaacgaa cgcaatgcc aacgccttg acttaccag ggagtaaca gatatgaaa tttttggcg cgccgttac gcttcaaag tttgacgag ttgttgcga gaaccctat aacgaaatg acctacagt tttcaatat actgacact gatcggact cacgatagc taccaggct tacattgaa gggcttttc aaggaaaaa gcttatcaa gttcatgct aatgttttt aataatgac agactgatc aagcctaca cgatcatgc aacgcctgg ctgagagaa gtggaccaa aatccgcaa acattcaat gaatacatg aaatttaaa cacaaccga gagtatcag caaatggtc agagacgtg ttccgaaga ctaggtttc agatacaca ctgaatgac actgtttta tccgatatg tacgacatg tgccgcttt gaaaaagct tggaatttg gatgctatg tctccttgg tgcatagtt tttaacaaa gaacaacta aaacttcta gaatacgcc gaagatttg aaatactat tacaaaagc ggttacggc aatgaagtt aaccgacaa attggttgt ccacctgtg aaagatctt tacgaaaaa ttcgaacgt acagtcaat aacggggga acccccact ggaaacaag gttacagtt ttcttcact cattctgtg acgattcag acgttttta acggcgatg ggtattgca aaggaccat caaccatta actgcggaa aattactac cagcagcaa caccgcaag tggcgtact tcgaaaatt gatccgttt gcgtcaaat ttggctgca gttttatat caatgccgt agcggagaa cgttatcgc gtcatgttc ttcttgaac gaggaacca gtgaattat ccagaatgt tcagtcggt ctttgcaac tggagtacc gtccaaaat aaattgcga ggtgttgta gacaattgt aatttagat ttctgcgat cgtaacgct gcaactgcc gtccaatac aattacatc atttatttc tcaagtctc atcgccatg tttctaagt gttaaaatc taaatgtta agttaggtt agtacaaag ttactgtta cttattttg tatatttta ttgttgatg tcatgctgt acaaaagga taggacccg ccatttgtt cgtaattgg tgccagtgt gaagcaaaa atgtgtcta agtagtttt agaaataaa aatacagtt ttttaacag aa
