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Gene information
General information
TC006138 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 3069205..3085451
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006138
    iBeetle screen iB_01000
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 10.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006138 RA
      MKSWLVCAF MLACLELGE SYSKYGRTC KDIGCLTNE 
      VCVMETDPC SYYQRQNEC GSYPTCKKM DNAGPPTCK 
      SYVCPPTKV CRMDGATPK CVDDHSKVG RVGYDTTEL 
      KSADQPSGN IYPNLPRGE TTPRPNIGY QGPPGQGYQ 
      GGQIGSGYP GQAKPYPQG YPQQTYPQQ PYPGYNGYN 
      NQQGGYRGG YPQQNGYPN YNYNGQYPN NYQGGYNPY 
      GQNRYGQNS GASSGGFVD SLTSALKKY GTNLLKDAI 
      TKQIAGGNN RN
    • >TC006138 RB
      MKSWLVCAF MLACLELGE SYSKYGRTC KDIGCLTNE 
      VCVMETDPC SYYQRQNEC GSYPTCKKM DNAGPPTCK 
      SYVCPPTKV CRMDGATPK CVDDHSKVG RVGYDTTEL 
      KSADQPSGN IYPNLPRGE TTPRPNIGY QGPPGQGYQ 
      GGQIGSGYP GQAKPYPQG YPQQTYPQQ PYPGYNGYN 
      NQQGGYRGG YPQQNGYPN YNYNGQYPN NYQGGYNPY 
      GQNRYGQNS GASSGGFVD SLTSALKKY GLFYMIGAF 
      YYQRIWG
    CDS sequence
    • >TC006138 RA
      atgaagtcg tggctcgta tgtgcattc atgctcgcg 
      tgcctggaa ttaggagaa tcttattca aaatatgga 
      cgcacgtgt aaagacatc ggttgtctc accaacgaa 
      gtatgcgta atggaaact gacccttgt tcttattac 
      caaagacaa aatgaatgt ggatcttac cccacttgt 
      aagaaaatg gacaatgct ggccctccg acgtgtaaa 
      agctacgtc tgtccgccc acgaaagta tgcagaatg 
      gacggcgcc acccctaaa tgtgtcgac gatcacagc 
      aaagtgggc cgtgtcggg tatgacacc accgagcta 
      aaaagcgcc gaccaaccc tctggaaac atctacccc 
      aaccttccc cgtggtgaa acaacccct cgacccaac 
      atcgggtac caaggacct cccggtcaa ggttaccaa 
      ggcggtcaa atcggttcg ggttaccct ggccaggcc 
      aaaccgtac ccacagggt tacccccaa caaacctac 
      ccccaacaa ccctacccg ggttacaac gggtataac 
      aaccagcaa gggggttac cgaggaggg tatccacaa 
      caaaacggt taccctaac tataactac aacggccag 
      tacccgaat aattaccaa gggggttac aacccttat 
      ggacagaac agatacgga cagaatagt ggagcttct 
      tccggcggt ttcgtcgat tcgcttaca agcgctttg 
      aagaaatat ggtacgaac ttgctcaaa gacgctata 
      accaagcag attgccggt ggtaataat cgtaattaa
    • >TC006138 RB
      atgaagtcg tggctcgta tgtgcattc atgctcgcg 
      tgcctggaa ttaggagaa tcttattca aaatatgga 
      cgcacgtgt aaagacatc ggttgtctc accaacgaa 
      gtatgcgta atggaaact gacccttgt tcttattac 
      caaagacaa aatgaatgt ggatcttac cccacttgt 
      aagaaaatg gacaatgct ggccctccg acgtgtaaa 
      agctacgtc tgtccgccc acgaaagta tgcagaatg 
      gacggcgcc acccctaaa tgtgtcgac gatcacagc 
      aaagtgggc cgtgtcggg tatgacacc accgagcta 
      aaaagcgcc gaccaaccc tctggaaac atctacccc 
      aaccttccc cgtggtgaa acaacccct cgacccaac 
      atcgggtac caaggacct cccggtcaa ggttaccaa 
      ggcggtcaa atcggttcg ggttaccct ggccaggcc 
      aaaccgtac ccacagggt tacccccaa caaacctac 
      ccccaacaa ccctacccg ggttacaac gggtataac 
      aaccagcaa gggggttac cgaggaggg tatccacaa 
      caaaacggt taccctaac tataactac aacggccag 
      tacccgaat aattaccaa gggggttac aacccttat 
      ggacagaac agatacgga cagaatagt ggagcttct 
      tccggcggt ttcgtcgat tcgcttaca agcgctttg 
      aagaaatat gggctattc tatatgatt ggtgcgttt 
      tattatcag aggatttgg ggatga
    mRNA sequence
    • >TC006138 RA
      tggttttaa tgacgtcaa gctaaatcg ttggcagaa 
      atcaaaggt tgttctcag ttgattctt tgtagtggt 
      agacagcag tgcaccaca tttttgttg acagttgtg 
      acaacgtga taaacgaaa aacaattga ccaggctct 
      tcaagatga agtcgtggc tcgtatgtg cattcatgc 
      tcgcgtgcc tggaattag gagaatctt attcaaaat 
      atggacgca cgtgtaaag acatcggtt gtctcacca 
      acgaagtat gcgtaatgg aaactgacc cttgttctt 
      attaccaaa gacaaaatg aatgtggat cttacccca 
      cttgtaaga aaatggaca atgctggcc ctccgacgt 
      gtaaaagct acgtctgtc cgcccacga aagtatgca 
      gaatggacg gcgccaccc ctaaatgtg tcgacgatc 
      acagcaaag tgggccgtg tcgggtatg acaccaccg 
      agctaaaaa gcgccgacc aaccctctg gaaacatct 
      accccaacc ttccccgtg gtgaaacaa cccctcgac 
      ccaacatcg ggtaccaag gacctcccg gtcaaggtt 
      accaaggcg gtcaaatcg gttcgggtt accctggcc 
      aggccaaac cgtacccac agggttacc cccaacaaa 
      cctaccccc aacaaccct acccgggtt acaacgggt 
      ataacaacc agcaagggg gttaccgag gagggtatc 
      cacaacaaa acggttacc ctaactata actacaacg 
      gccagtacc cgaataatt accaagggg gttacaacc 
      cttatggac agaacagat acggacaga atagtggag 
      cttcttccg gcggtttcg tcgattcgc ttacaagcg 
      ctttgaaga aatatggta cgaacttgc tcaaagacg 
      ctataacca agcagattg ccggtggta ataatcgta 
      attaatttt ctgtgttac agggctatt ctatatgat 
      tggtgcgtt ttattatca gaggatttg gggatgatt 
      ttgtaacgt taacataat tttatgtgt tgatatttt 
      ttaataaag tttgaaaat cctttgagt gtttccttg 
      gtgagtatt tggtggtag tctgtcact aactgcaca 
      gctgagaat agcctttta acacaactt cttgccatt 
      ttctatgtg ttgtgtttt tctaaagta atacaagta 
      ggtacgaaa ttaatttgt atgctaata agctgcctt 
      ctagcccct tctcctcat tttcctccg cttgatgta 
      gtagttttt gtatttatt ttgtttgtc cgataataa 
      attaaaata cgtccc
    • >TC006138 RB
      tggttttaa tgacgtcaa gctaaatcg ttggcagaa 
      atcaaaggt tgttctcag ttgattctt tgtagtggt 
      agacagcag tgcaccaca tttttgttg acagttgtg 
      acaacgtga taaacgaaa aacaattga ccaggctct 
      tcaagatga agtcgtggc tcgtatgtg cattcatgc 
      tcgcgtgcc tggaattag gagaatctt attcaaaat 
      atggacgca cgtgtaaag acatcggtt gtctcacca 
      acgaagtat gcgtaatgg aaactgacc cttgttctt 
      attaccaaa gacaaaatg aatgtggat cttacccca 
      cttgtaaga aaatggaca atgctggcc ctccgacgt 
      gtaaaagct acgtctgtc cgcccacga aagtatgca 
      gaatggacg gcgccaccc ctaaatgtg tcgacgatc 
      acagcaaag tgggccgtg tcgggtatg acaccaccg 
      agctaaaaa gcgccgacc aaccctctg gaaacatct 
      accccaacc ttccccgtg gtgaaacaa cccctcgac 
      ccaacatcg ggtaccaag gacctcccg gtcaaggtt 
      accaaggcg gtcaaatcg gttcgggtt accctggcc 
      aggccaaac cgtacccac agggttacc cccaacaaa 
      cctaccccc aacaaccct acccgggtt acaacgggt 
      ataacaacc agcaagggg gttaccgag gagggtatc 
      cacaacaaa acggttacc ctaactata actacaacg 
      gccagtacc cgaataatt accaagggg gttacaacc 
      cttatggac agaacagat acggacaga atagtggag 
      cttcttccg gcggtttcg tcgattcgc ttacaagcg 
      ctttgaaga aatatgggc tattctata tgattggtg 
      cgttttatt atcagagga tttggggat gattttgta 
      acgttaaca taattttat gtgttgata ttttttaat 
      aaagtttga aaatccttt gagtgtttc cttggtgag 
      tatttggtg gtagtctgt cactaactg cacagctga 
      gaatagcct tttaacaca acttcttgc cattttcta 
      tgtgttgtg tttttctaa agtaataca agtaggtac 
      gaaattaat ttgtatgct aataagctg ccttctagc 
      cccttctcc tcattttcc tccgcttga tgtagtagt 
      ttttgtatt tattttgtt tgtccgata ataaattaa 
      aatacgtcc ctgccggag tttcatttc cccggaata 
      aattactca cgcactc