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Gene information
General information
TC006114 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 2291177..2295696
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006114
    iBeetle screen iB_00998
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 50.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006114 RA
      MSDSDDNAN NLPDGQMEN GHISVDSQF NFSRERHSG 
      TNQASDQFD IDLPTSHRY LGKLDNVTG YTFYDDGEV 
      ATLAAIQTN TLVFPGFTL PLVLNNDLE INMLQRHTS 
      KKNAFVLLT PDKTFKGFF TYGVIMEIF ETHLRNSVL 
      YLKARSRQR CRLVPGKEI KTLIGDLYQ VTVEVMLEP 
      PPTSPLCNT QLNSLKIRK LFNCTEYRE FYKYRKFRL 
      FHLSQFPLA SWVYDKHEI CFLHHKLVN ALSNYYLIE 
      FIPDDPVQL SYWFVQNFQ LRHEERLRI MVLRTAVER 
      LRLELKYLN MKRLICCAK CDAEIAQHS NVFPMSKDG 
      VQSNYCNPE GYVFETVTV SKATNFTLF GSPSAQFSW 
      FPGYSWTIM HCKMCSSHL GWKFSSVNL KPAVFYGLA 
      KSGFDIKIV KEESEVL
    CDS sequence
    • >TC006114 RA
      atgtcggac tcggacgac aacgcgaac aatttgccc 
      gacgggcag atggaaaac gggcacatt tcagtcgac 
      tcgcagttc aatttttca cgcgagaga cactctggc 
      acgaaccaa gcgtccgac cagttcgat attgattta 
      ccgacctcg caccgttat ttgggcaag ttggacaat 
      gtcacaggt tacacgttt tacgacgac ggtgaggtc 
      gccactttg gcggccatt cagacaaac acgctggtt 
      ttccccggt ttcacgtta ccgctagtg ttaaataac 
      gatctggag ataaacatg ctacagaga cacacttcg 
      aagaaaaac gcctttgtt ttactgacc cctgacaaa 
      acttttaag ggctttttc acgtacggg gtcattatg 
      gagattttc gagacgcat ttgcgcaac agtgtgctc 
      tatttgaag gcgaggagt cggcaaagg tgccgatta 
      gttcccggg aaggagatt aaaaccctg atcggtgac 
      ttgtatcaa gtcaccgtg gaggtcatg ctggagcca 
      ccgccaact tcgcccctt tgcaacaca cagctgaac 
      tcgcttaaa atccgcaaa ctgttcaac tgtacggaa 
      tatagagag ttttacaag taccgcaag ttcagattg 
      tttcacctc agccagttt ccgctagcg agctgggtt 
      tatgacaaa cacgagatt tgctttctc caccataaa 
      cttgtaaac gcgttgtct aactattat ttaatcgag 
      tttattccg gacgatccg gtgcagttg tcgtattgg 
      tttgtgcag aactttcag ttaaggcac gaggaaaga 
      ctgaggatt atggtgttg aggacggcc gttgagcgg 
      ctgaggctt gaactcaaa tatctcaac atgaaaaga 
      ctgatttgt tgtgcgaaa tgtgacgcg gaaattgcg 
      cagcatagt aacgttttt ccaatgtcg aaggacggc 
      gttcagagc aattactgc aatccggaa ggatacgtt 
      tttgagacg gtgacggtc tcaaaagct acaaatttc 
      acacttttt gggtcgcct tcggctcag ttttcgtgg 
      ttccctggg tattcctgg actatcatg cattgtaaa 
      atgtgcagt tcgcacctg gggtggaaa ttctcgtcg 
      gtgaatcta aaaccggcc gttttttac ggccttgcc 
      aaaagtggt ttcgatatt aagatcgtg aaagaggaa 
      tctgaggtt ttgtaa
    mRNA sequence
    • >TC006114 RA
      ttttgtcaa taattgagg tgttattta cacgcaaaa 
      ttgcaaaac aacaaataa tagaccatg tcggactcg 
      gacgacaac gcgaacaat ttgcccgac gggcagatg 
      gaaaacggg cacatttca gtcgactcg cagttcaat 
      ttttcacgc gagagacac tctggcacg aaccaagcg 
      tccgaccag ttcgatatt gatttaccg acctcgcac 
      cgttatttg ggcaagttg gacaatgtc acaggttac 
      acgttttac gacgacggt gaggtcgcc actttggcg 
      gccattcag acaaacacg ctggttttc cccggtttc 
      acgttaccg ctagtgtta aataacgat ctggagata 
      aacatgcta cagagacac acttcgaag aaaaacgcc 
      tttgtttta ctgacccct gacaaaact tttaagggc 
      tttttcacg tacggggtc attatggag attttcgag 
      acgcatttg cgcaacagt gtgctctat ttgaaggcg 
      aggagtcgg caaaggtgc cgattagtt cccgggaag 
      gagattaaa accctgatc ggtgacttg tatcaagtc 
      accgtggag gtcatgctg gagccaccg ccaacttcg 
      cccctttgc aacacacag ctgaactcg cttaaaatc 
      cgcaaactg ttcaactgt acggaatat agagagttt 
      tacaagtac cgcaagttc agattgttt cacctcagc 
      cagtttccg ctagcgagc tgggtttat gacaaacac 
      gagatttgc tttctccac cataaactt gtaaacgcg 
      ttgtctaac tattattta atcgagttt attccggac 
      gatccggtg cagttgtcg tattggttt gtgcagaac 
      tttcagtta aggcacgag gaaagactg aggattatg 
      gtgttgagg acggccgtt gagcggctg aggcttgaa 
      ctcaaatat ctcaacatg aaaagactg atttgttgt 
      gcgaaatgt gacgcggaa attgcgcag catagtaac 
      gtttttcca atgtcgaag gacggcgtt cagagcaat 
      tactgcaat ccggaagga tacgttttt gagacggtg 
      acggtctca aaagctaca aatttcaca ctttttggg 
      tcgccttcg gctcagttt tcgtggttc cctgggtat 
      tcctggact atcatgcat tgtaaaatg tgcagttcg 
      cacctgggg tggaaattc tcgtcggtg aatctaaaa 
      ccggccgtt ttttacggc cttgccaaa agtggtttc 
      gatattaag atcgtgaaa gaggaatct gaggttttg 
      taattacga gtattcgtt gaatggtga tgatgaagt 
      aggggctgt gaaatgcta ttcattgtg taattctca 
      cctaattag gaaacacgt gtatttagg tgtaaggtc 
      gcaataaga gaacagtga gtatttcaa gatttattt 
      taaaaaaat aataaagcg cttatgtat ataaatatt 
      tgccatcta ataataacg aacctgttc agctctctc 
      tccggtaaa aaaataata aaggttaga ttaacttaa 
      caaaaataa atagtttcc atgcga