Status
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Gene information
General information
TC006108 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2514418..2523513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006108
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00996

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006108 RA
      MGDVSTTKS TLAQNEPAM KGWLFKWTN YLKGYQRRW 
      FVLQNGHLS YYRAENNGG VPTLSIRRR RRFKGNQAE 
      MAHTCRGSI SLHGALIYT VDACTFVIS NGGTQTFHI 
      RAASEVERQ SWVTALELA KAKAIRSME SEDDEEETD 
      EGSGSPEDW SSVVRKLEA QLNDIQTCS DLIQRHWKS 
      LVKPLTEME TNLDPDSVQ AKSKEVSER ATLFRISTN 
      AMINACGEY LKTAQTHGH KWIRLLQHE REQRQRLQE 
      MVETLAQQH SKLEQAANA HTNRPTVNP SDGEEEDEN 
      EFYDAVADS TAGVSDGHF TLNIPTGTG HRRNSSDSS 
      SEPDETTET KQVVVVTGK STNQARKVQ QSDSHVATI 
      STSKRQRRT RVPDKPHYP LNLWSIMKN CIGKDLSKI 
      PMPVNFSEP LSMLQRLTE DYEYAETLD VAAKCTDPC 
      EQLAYVAAF TISAYSTTS SRTGKPFNP LLGETYECD 
      RRDDLGWRA FNEQVSHHP PMVAQYCEG RDWKCWQEF 
      TMTSKFRGK YLQVIPLGT AQVEFDNGN KFSWRKVTT 
      TIHNIIVGK LWVDQHGDM EIIGKGAAA GIKCHLKYI 
      PYSYFTRDT QRRVKGVVM DVGGNVKWI INGTWDNQV 
      EIAPVTGST GSSSNPVYE TGPSVVAWK RRMPPPDAD 
      KYYGFTLLA AQLNEPEEG VAPTDSRLR PDQRLMEEG 
      RWDEANTEK VRLEEKQRA VRRRREAEA EKAAAEGRP 
      FEHYKPIWF EQVTDEDGV VVHVYKGDY WECKDMQTW 
      DRCPDIF
    CDS sequence
    • >TC006108 RA
      atgggcgac gtatcgacg acaaagtcc accctggcc 
      cagaacgag ccagccatg aagggctgg ttgttcaaa 
      tggaccaac tatctgaaa ggttaccaa cggcgatgg 
      ttcgtcctc caaaatgga catctgtca tactacagg 
      gccgagaat aacggaggg gtccccact ctgtcaatt 
      cggagacgt agaaggttc aaggggaac caggccgag 
      atggcgcac acctgccgc gggagcatc tccctgcac 
      ggggccctg atctacacc gtggacgca tgcacgttc 
      gtcatttcg aacgggggc acccagacg ttccacatc 
      cgggcggcg tccgaggtg gagcgccag tcgtgggtc 
      acggccctg gagctggcc aaggccaag gcaattcgc 
      agcatggag tcggaggac gacgaggag gagactgat 
      gaggggtcc ggcagcccc gaagactgg tccagtgtc 
      gtgcgcaag ttggaggcg caactcaac gacatccag 
      acgtgcagc gacctcatc cagaggcac tggaagtcg 
      ctggtcaag cctctgact gagatggag acgaatctg 
      gaccccgat agtgtccag gccaagtcg aaggaggtc 
      agcgaaagg gccactctc ttccggatc agcaccaac 
      gctatgata aacgcctgt ggcgagtat ttgaaaaca 
      gcacagaca cacggccac aagtggatc aggcttttg 
      caacatgaa cgggaacaa agacaaagg ttgcaagaa 
      atggtcgag accttagcc cagcaacat tcgaagttg 
      gagcaggcg gctaacgcc cacacgaat cggcccaca 
      gtgaatccg agcgatggg gaggaggag gacgaaaat 
      gagttttac gacgctgta gctgatagt acagcgggt 
      gtttcagac gggcatttt actttgaat ataccgacg 
      gggacggga catcgtagg aacagttcc gatagttcg 
      agtgaacca gatgaaacg actgaaact aaacaagtg 
      gtggtggtc actggcaaa tcgacaaac caagcacgc 
      aaagtccaa cagagcgac agccacgtc gccacaatt 
      tccaccagc aaaagacag aggcggact cgggtcccc 
      gacaagcct cactatcca ctcaaccta tggagcata 
      atgaaaaat tgcatcgga aaagactta tcgaaaatt 
      ccgatgcct gtcaatttc agcgaacct ctatcaatg 
      ctccaacgt ctcaccgaa gactacgaa tacgccgaa 
      acgctcgac gttgctgca aaatgcacc gatccgtgc 
      gaacaactg gcatacgtt gccgccttc acaatctcc 
      gcctattca acgaccagt tcgcgaact ggtaaaccg 
      ttcaacccc ctcttgggg gagacgtac gagtgcgat 
      aggagagac gacttgggc tggagggct ttcaacgaa 
      caagtatcg catcatccg ccgatggtg gcgcagtat 
      tgcgaaggc cgcgactgg aagtgttgg caagagttt 
      acgatgacg tcgaagttt agggggaag tacctgcag 
      gtgataccg ctagggacg gcacaagtc gagttcgat 
      aatggtaat aagttttcg tggaggaaa gtcaccact 
      acgatacat aacatcatc gtgggcaaa ttatgggtg 
      gatcagcat ggtgatatg gagattatc gggaaaggg 
      gctgcggcc gggatcaag tgtcattta aaatacata 
      ccgtattcg tatttcaca agagacacg cagaggcgg 
      gtcaagggg gtcgtgatg gatgtgggg gggaacgtc 
      aagtggatt atcaatggc acttgggac aaccaagtt 
      gagatagcc cccgtgacg ggtagtaca gggagctcg 
      agtaatccc gtgtacgag acggggccg tcggtggtg 
      gcgtggaag cggcgcatg ccgcccccc gacgcggat 
      aagtactac ggttttacg cttctcgct gctcaattg 
      aacgaaccg gaggagggc gtagccccg accgattcg 
      agactgcgc cccgatcaa cgacttatg gaagagggg 
      cgttgggac gaggcaaat accgagaaa gtgcgcctt 
      gaggagaag caaagggcc gttaggagg cggcgcgag 
      gcggaggcg gagaaggcg gcggcagaa gggaggccc 
      tttgagcat tataagccg atctggttt gagcaagtc 
      actgatgag gatggggtt gttgttcat gtctataaa 
      ggcgattat tgggagtgt aaggatatg caaacgtgg 
      gatcggtgt cctgatata ttctag
    mRNA sequence
    • >TC006108 RA
      cttggacga atctattca ttggaggca cacttttaa 
      gtcaacaca gtcacaaag acgcgcttt taaggcaat 
      tatttgggg ctaaaaccg ggcccaact caccggtaa 
      caattgaat ttggaacta gcggtgcca accttgcgc 
      cactcacgc ccctagaat ttcgtgcgt aaattgctc 
      aaacaattc gaatttaat taaaattgg gtgagtacg 
      gtggtgatt gtccctggg gcgtgtttg gccgccgtg 
      tccggctga tttcggccg ttttggccc cccatatgt 
      gactgtaat tagggttac gtcacttgg tttaaaaga 
      tggacgacg tgtcattgt gagtaccaa ataaaaaca 
      attctggta ccaaactag cgaacaatg ggcgacgta 
      tcgacgaca aagtccacc ctggcccag aacgagcca 
      gccatgaag ggctggttg ttcaaatgg accaactat 
      ctgaaaggt taccaacgg cgatggttc gtcctccaa 
      aatggacat ctgtcatac tacagggcc gagaataac 
      ggaggggtc cccactctg tcaattcgg agacgtaga 
      aggttcaag gggaaccag gccgagatg gcgcacacc 
      tgccgcggg agcatctcc ctgcacggg gccctgatc 
      tacaccgtg gacgcatgc acgttcgtc atttcgaac 
      gggggcacc cagacgttc cacatccgg gcggcgtcc 
      gaggtggag cgccagtcg tgggtcacg gccctggag 
      ctggccaag gccaaggca attcgcagc atggagtcg 
      gaggacgac gaggaggag actgatgag gggtccggc 
      agccccgaa gactggtcc agtgtcgtg cgcaagttg 
      gaggcgcaa ctcaacgac atccagacg tgcagcgac 
      ctcatccag aggcactgg aagtcgctg gtcaagcct 
      ctgactgag atggagacg aatctggac cccgatagt 
      gtccaggcc aagtcgaag gaggtcagc gaaagggcc 
      actctcttc cggatcagc accaacgct atgataaac 
      gcctgtggc gagtatttg aaaacagca cagacacac 
      ggccacaag tggatcagg cttttgcaa catgaacgg 
      gaacaaaga caaaggttg caagaaatg gtcgagacc 
      ttagcccag caacattcg aagttggag caggcggct 
      aacgcccac acgaatcgg cccacagtg aatccgagc 
      gatggggag gaggaggac gaaaatgag ttttacgac 
      gctgtagct gatagtaca gcgggtgtt tcagacggg 
      cattttact ttgaatata ccgacgggg acgggacat 
      cgtaggaac agttccgat agttcgagt gaaccagat 
      gaaacgact gaaactaaa caagtggtg gtggtcact 
      ggcaaatcg acaaaccaa gcacgcaaa gtccaacag 
      agcgacagc cacgtcgcc acaatttcc accagcaaa 
      agacagagg cggactcgg gtccccgac aagcctcac 
      tatccactc aacctatgg agcataatg aaaaattgc 
      atcggaaaa gacttatcg aaaattccg atgcctgtc 
      aatttcagc gaacctcta tcaatgctc caacgtctc 
      accgaagac tacgaatac gccgaaacg ctcgacgtt 
      gctgcaaaa tgcaccgat ccgtgcgaa caactggca 
      tacgttgcc gccttcaca atctccgcc tattcaacg 
      accagttcg cgaactggt aaaccgttc aaccccctc 
      ttgggggag acgtacgag tgcgatagg agagacgac 
      ttgggctgg agggctttc aacgaacaa gtatcgcat 
      catccgccg atggtggcg cagtattgc gaaggccgc 
      gactggaag tgttggcaa gagtttacg atgacgtcg 
      aagtttagg gggaagtac ctgcaggtg ataccgcta 
      gggacggca caagtcgag ttcgataat ggtaataag 
      ttttcgtgg aggaaagtc accactacg atacataac 
      atcatcgtg ggcaaatta tgggtggat cagcatggt 
      gatatggag attatcggg aaaggggct gcggccggg 
      atcaagtgt catttaaaa tacataccg tattcgtat 
      ttcacaaga gacacgcag aggcgggtc aagggggtc 
      gtgatggat gtggggggg aacgtcaag tggattatc 
      aatggcact tgggacaac caagttgag atagccccc 
      gtgacgggt agtacaggg agctcgagt aatcccgtg 
      tacgagacg gggccgtcg gtggtggcg tggaagcgg 
      cgcatgccg ccccccgac gcggataag tactacggt 
      tttacgctt ctcgctgct caattgaac gaaccggag 
      gagggcgta gccccgacc gattcgaga ctgcgcccc 
      gatcaacga cttatggaa gaggggcgt tgggacgag 
      gcaaatacc gagaaagtg cgccttgag gagaagcaa 
      agggccgtt aggaggcgg cgcgaggcg gaggcggag 
      aaggcggcg gcagaaggg aggcccttt gagcattat 
      aagccgatc tggtttgag caagtcact gatgaggat 
      ggggttgtt gttcatgtc tataaaggc gattattgg 
      gagtgtaag gatatgcaa acgtgggat cggtgtcct 
      gatatattc tagtaataa atgaagctt gattaaata 
      attttttta aaccgaggc tgaggtttt tttcccggg 
      tacggtatt ttaagtttt atgctacaa gtcaatgtt 
      ggaaacaat catatatcc tccttgtat atattcgtt 
      gtattttta gccagtttt agaatgtgg aacaagaaa 
      tttactcag ttttttttt tttgtaaaa agtcacttt 
      ccgcaatta tgtgatacc agagaatgt acataacgt 
      tacattgtt ttgtgtagt ggttttgag agtatggaa 
      aatcagatt gttgtatat attgtatca atcaatgct 
      ttgcccgat gtagaattc aataaattt tctaatgca 
      tc
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