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Gene information
General information
TC006065 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2069611..2088075
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006065
    iBeetle screen iB_00990

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006065 RA
      MAYRRLFYI VFLLVILLP RDFCYPGSK NLRPLCPRL 
      YERTFHGFV PQGNLTAGT FKEIEGVSK LKDCVLKCC 
      LQKQCNVAF MTDEKCYHI TCASDELCK PVLSLNPDS 
      KDRVSMVLV KPTDEDSWQ DVLAQDFST EHLDREVYN 
      VLNDKRRLQ DIYKDLVMD ESTYNYDLS CEVGTDNGC 
      APNEICVQN YPKSRAGTC TCKPRFIRN HSGDCVLLD 
      LKDMGSHSS EIMTERILD LANKSAPVT RKHLSVSAE 
      NKIVKLPDN EVTLIANVS PDDSNEDEK YQYEWTSLQ 
      QPDGSTAVK HQNGGQLQL SKLAEGLYT FKVSVSNSF 
      AYGETFVNV TVLPPSRIN KPPQIVITP ANQVIKLPN 
      NAAVLDAST STDDDGIVS WHWELQQGP LGYQPELKD 
      TATLQLNDL KKPGNYTFK LTVTDTDKA TSSATANVT 
      VLKFTDYPP DANAGEDKI IYLPHNNIT LNGNLSTDD 
      HAITTWEWT KSPDDAQKA VDMQDTRTP YLQLSNLEE 
      GMYTFSLKV TDSAGQSST AQVHVFVKP PTNKPPIAE 
      AGSNITISL PQTWVVLDA TNSSDDNKI TAFKWEQVE 
      GPSKVEFAN SSLSKTNVT GLTKGSYIF KVSVTDDNN 
      NVASDTVTV TVNQNVNQK PKANAGGDF EVELPLNAV 
      LVNGSKSKD DWAIVKWKW TRDDKSLAI GNVAEKSDE 
      SPILILTDV IAGKYIFNL TVFDEQGLS DTDTVTFTV 
      KNDPKLLNL VEITIDINA KHLSQAQYN TLTGKLALL 
      VIDGSKLKV RNVKPEVGS GKAMIIFYV ETPDGTAYP 
      ANDIVQHLR EKLRVDAGL LGFSVTKLQ TAICQNNCS 
      GHGVCNEQT RKCECEAFW MQDLFKVYL KTDEDSDCS 
      WSILYVVLG VVCTVLAFF GAMWGLVYL CYNWCSRRW 
      TGSKPTTYK LIEDLPPLV PRKGNLSDS DTDSDVVFE 
      SRSKNSRFS DSRNGHKPS RNGFIKGGR RVKT
    CDS sequence
    • >TC006065 RA
      atggcatac agacgctta ttttacatt gttttcctt 
      cttgtgata ctcttacca cgggatttt tgttatccg 
      gggtcgaag aatttacgc ccgttatgt ccaaggttg 
      tatgaaaga acgtttcat gggttcgtg ccccaaggc 
      aatctcacg gcaggtaca ttcaaggag attgaaggt 
      gttagcaag ttgaaagat tgtgtcctt aaatgttgt 
      ctgcagaaa cagtgcaat gttgcgttt atgaccgat 
      gagaaatgt tatcatatc acttgtgct agtgacgaa 
      ttgtgcaaa cctgtgttg agtttaaat cctgactca 
      aaggaccgc gtttcgatg gttcttgtg aagccgact 
      gacgaggat tcgtggcag gatgttttg gctcaagat 
      ttttcgact gaacatttg gaccgggaa gtttacaac 
      gtgttgaac gacaagaga agactgcag gacatctac 
      aaagatcta gtaatggac gaatccact tataattat 
      gatctcagc tgtgaagtg ggtacagat aatggatgt 
      gcccctaat gaaatttgc gtccaaaat tacccaaaa 
      tcgcgtgct ggaacgtgc acttgcaaa ccgcgattt 
      attcgaaac cattccggt gattgtgtt cttctcgac 
      ctaaaagat atgggatca cacagcagt gaaatcatg 
      actgagcga atcttagat cttgcaaat aagtcggct 
      cctgtcact cgtaaacac ttatcagtg agtgccgaa 
      aacaaaatt gtgaaattg ccagataat gaagtaact 
      ttgatcgcc aatgtctcg ccggatgat tcgaacgag 
      gacgaaaaa tatcagtac gaatggacg tcgttacaa 
      caaccagat ggcagcact gcggttaag catcaaaat 
      ggcgggcaa cttcagttg tcaaagttg gcagaagga 
      ttgtatact tttaaagtc agcgtttcg aattcgttt 
      gcttatggc gaaacgttt gtcaatgtg acagtctta 
      ccaccttca cggattaat aaaccgcca caaattgtt 
      attacgcca gctaatcaa gttattaag ttgccaaat 
      aatgcggct gttttggat gcttcaaca tcgactgat 
      gatgatggg atcgtgtcg tggcactgg gagctgcaa 
      caaggaccg ttaggttat caaccggag ctcaaagac 
      acagcgact ttacagttg aacgatttg aaaaaacct 
      gggaattac acgttcaag ttaacagta acagataca 
      gacaaggct accagctcc gctacagcc aacgtaaca 
      gtcttaaaa ttcaccgat tatccacca gatgcaaac 
      gctggtgaa gataaaatt atctatttg ccgcacaac 
      aacataaca ttaaacggc aatctcagc accgatgat 
      catgcaatc acgacttgg gaatggaca aaaagtccc 
      gacgatgct caaaaagct gttgatatg caagatacg 
      agaacccct tatcttcaa ctatcaaat ttagaggaa 
      ggaatgtat acgttttct ttaaaggtg accgatagt 
      gcgggccaa agcagtaca gctcaagtt catgttttt 
      gtgaaacct ccaactaat aaacctcca attgctgaa 
      gctggaagc aatataacg attagtttg cctcagact 
      tgggttgtt ttggatgct acgaatagt tcagatgat 
      aataaaatt actgctttt aaatgggaa caagttgag 
      ggaccttca aaagtcgaa tttgccaac agtagtttg 
      agtaaaaca aatgttacg ggactaacg aagggaagc 
      tacatattt aaagtcagc gttacggat gataataac 
      aatgttgcc agcgatact gtaacggtc actgtgaat 
      cagaatgtc aatcagaag cccaaagct aacgccggc 
      ggagacttt gaagtcgaa ttgcccctc aatgccgtc 
      ttagtcaac ggttcaaaa tcaaaagat gattgggcc 
      atagttaaa tggaaatgg acccgcgac gataaatcc 
      cttgccata ggaaacgta gctgaaaaa tctgacgaa 
      tcaccgatt ctaatttta acagacgtt atcgccggg 
      aaatacatc ttcaacttg accgtgttc gatgaacaa 
      ggtcttagc gatacagac actgttaca ttcaccgtc 
      aaaaatgac ccaaaactt ctcaacctc gtcgaaatc 
      accatcgac ataaacgcc aaacacctc tcacaagcc 
      caatacaac actctcacc ggaaaactc gcacttctt 
      gttatcgac ggttcgaag ttaaaagtg cgaaacgtt 
      aaacccgaa gtgggttcc ggcaaagca atgattatt 
      ttctatgtg gaaacaccc gatggcact gcatatccg 
      gcaaatgat atcgtgcaa catctcagg gagaagttg 
      cgagttgat gcgggactt ttgggtttt tcggtgacc 
      aagttgcag acggcaatt tgtcagaac aattgctca 
      gggcatggt gtttgtaac gagcagacg aggaaatgt 
      gaatgcgaa gcgttttgg atgcaggat ttgtttaaa 
      gtttatttg aaaactgat gaggattcg gattgtagt 
      tggagtatt ttgtatgtt gttttgggg gttgtttgt 
      actgttttg gccttcttt ggggcgatg tggggtttg 
      gtgtatcta tgctacaat tggtgttcg aggaggtgg 
      acgggctcg aagcctacg acgtacaag ttgattgaa 
      gacttacct ccacttgta ccacggaaa ggcaattta 
      tccgacagc gacaccgat tccgacgta gttttcgaa 
      tcaagatcg aagaattct cgatttagt gactcaaga 
      aacggccat aaaccgagt cgaaacggc tttatcaaa 
      ggagggcgt agggttaag acgtag
    mRNA sequence
    • >TC006065 RA
      ataaattca cgttgtgtt atcgagcct gtagttaaa 
      tgttaacaa tttccgcca acaaccatt ggaatggca 
      tacagacgc ttattttac attgttttc cttcttgtg 
      atactctta ccacgggat ttttgttat ccggggtcg 
      aagaattta cgcccgtta tgtccaagg ttgtatgaa 
      agaacgttt catgggttc gtgccccaa ggcaatctc 
      acggcaggt acattcaag gagattgaa ggtgttagc 
      aagttgaaa gattgtgtc cttaaatgt tgtctgcag 
      aaacagtgc aatgttgcg tttatgacc gatgagaaa 
      tgttatcat atcacttgt gctagtgac gaattgtgc 
      aaacctgtg ttgagttta aatcctgac tcaaaggac 
      cgcgtttcg atggttctt gtgaagccg actgacgag 
      gattcgtgg caggatgtt ttggctcaa gatttttcg 
      actgaacat ttggaccgg gaagtttac aacgtgttg 
      aacgacaag agaagactg caggacatc tacaaagat 
      ctagtaatg gacgaatcc acttataat tatgatctc 
      agctgtgaa gtgggtaca gataatgga tgtgcccct 
      aatgaaatt tgcgtccaa aattaccca aaatcgcgt 
      gctggaacg tgcacttgc aaaccgcga tttattcga 
      aaccattcc ggtgattgt gttcttctc gacctaaaa 
      gatatggga tcacacagc agtgaaatc atgactgag 
      cgaatctta gatcttgca aataagtcg gctcctgtc 
      actcgtaaa cacttatca gtgagtgcc gaaaacaaa 
      attgtgaaa ttgccagat aatgaagta actttgatc 
      gccaatgtc tcgccggat gattcgaac gaggacgaa 
      aaatatcag tacgaatgg acgtcgtta caacaacca 
      gatggcagc actgcggtt aagcatcaa aatggcggg 
      caacttcag ttgtcaaag ttggcagaa ggattgtat 
      acttttaaa gtcagcgtt tcgaattcg tttgcttat 
      ggcgaaacg tttgtcaat gtgacagtc ttaccacct 
      tcacggatt aataaaccg ccacaaatt gttattacg 
      ccagctaat caagttatt aagttgcca aataatgcg 
      gctgttttg gatgcttca acatcgact gatgatgat 
      gggatcgtg tcgtggcac tgggagctg caacaagga 
      ccgttaggt tatcaaccg gagctcaaa gacacagcg 
      actttacag ttgaacgat ttgaaaaaa cctgggaat 
      tacacgttc aagttaaca gtaacagat acagacaag 
      gctaccagc tccgctaca gccaacgta acagtctta 
      aaattcacc gattatcca ccagatgca aacgctggt 
      gaagataaa attatctat ttgccgcac aacaacata 
      acattaaac ggcaatctc agcaccgat gatcatgca 
      atcacgact tgggaatgg acaaaaagt cccgacgat 
      gctcaaaaa gctgttgat atgcaagat acgagaacc 
      ccttatctt caactatca aatttagag gaaggaatg 
      tatacgttt tctttaaag gtgaccgat agtgcgggc 
      caaagcagt acagctcaa gttcatgtt tttgtgaaa 
      cctccaact aataaacct ccaattgct gaagctgga 
      agcaatata acgattagt ttgcctcag acttgggtt 
      gttttggat gctacgaat agttcagat gataataaa 
      attactgct tttaaatgg gaacaagtt gagggacct 
      tcaaaagtc gaatttgcc aacagtagt ttgagtaaa 
      acaaatgtt acgggacta acgaaggga agctacata 
      tttaaagtc agcgttacg gatgataat aacaatgtt 
      gccagcgat actgtaacg gtcactgtg aatcagaat 
      gtcaatcag aagcccaaa gctaacgcc ggcggagac 
      tttgaagtc gaattgccc ctcaatgcc gtcttagtc 
      aacggttca aaatcaaaa gatgattgg gccatagtt 
      aaatggaaa tggacccgc gacgataaa tcccttgcc 
      ataggaaac gtagctgaa aaatctgac gaatcaccg 
      attctaatt ttaacagac gttatcgcc gggaaatac 
      atcttcaac ttgaccgtg ttcgatgaa caaggtctt 
      agcgataca gacactgtt acattcacc gtcaaaaat 
      gacccaaaa cttctcaac ctcgtcgaa atcaccatc 
      gacataaac gccaaacac ctctcacaa gcccaatac 
      aacactctc accggaaaa ctcgcactt cttgttatc 
      gacggttcg aagttaaaa gtgcgaaac gttaaaccc 
      gaagtgggt tccggcaaa gcaatgatt attttctat 
      gtggaaaca cccgatggc actgcatat ccggcaaat 
      gatatcgtg caacatctc agggagaag ttgcgagtt 
      gatgcggga cttttgggt ttttcggtg accaagttg 
      cagacggca atttgtcag aacaattgc tcagggcat 
      ggtgtttgt aacgagcag acgaggaaa tgtgaatgc 
      gaagcgttt tggatgcag gatttgttt aaagtttat 
      ttgaaaact gatgaggat tcggattgt agttggagt 
      attttgtat gttgttttg ggggttgtt tgtactgtt 
      ttggccttc tttggggcg atgtggggt ttggtgtat 
      ctatgctac aattggtgt tcgaggagg tggacgggc 
      tcgaagcct acgacgtac aagttgatt gaagactta 
      cctccactt gtaccacgg aaaggcaat ttatccgac 
      agcgacacc gattccgac gtagttttc gaatcaaga 
      tcgaagaat tctcgattt agtgactca agaaacggc 
      cataaaccg agtcgaaac ggctttatc aaaggaggg 
      cgtagggtt aagacgtag gcgaatcta caagagcac 
      aactctttc cttaccccg tgcctaatt acttaaata 
      gggtgtttg tatgtgtgt ttgatggaa atgtatcaa 
      ataactgtt gtattatct gcaagtgtt aaacacttt 
      tttttctat caggctgta atcaagtta cctatacac 
      tttatttat ttttttctt ttccacttt aattggggt 
      tgtgggatc tgttttaac attttttaa ttgatttaa 
      cggggtttt atttattgt tatacatag tttataact 
      atgcttaga tgtgtgtag aggaggtaa attagtcag 
      tgatgtagt gtaatttgc actttttca aaggaagtc 
      agtcagatt tttgtgata tgattaaaa agtatttta 
      agta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY