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Gene information
General information
TC006055 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2406856..2414846
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006055
    iBeetle screen iB_00988

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature dorsal partiallyrounded - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature lateral partiallyrounded - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    urogomphi size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006055 RA
      MSVEYLQVA EDEGEEPIE LPTEDDGTL LLSTLAAQF 
      PGSSGLKYR NPETKAMRG VRLSEGRLH PPPEIGWGT 
      QIFYCVFPK ENKRKSDDN LENSTAKTK RMETKLRCT 
      DLIVLGLPW KTTEQNLRE YFETFGEVL MAQVKKDPK 
      TGQSKGFGF IRFASYESQ MRVLAQRHM IDGRWCDVK 
      VPNSKEGLI QQVPCKVFI GRCTEELTA DDLREYFGK 
      YGEVTDVFI PKPFRAFSF VTFLDPEVA QSLCGEDHI 
      IKGVSVHVS NAAPKSETR GGGYGGGGP RGANHGNNR 
      SGPGPDGPG MYQQRYNPG PNQGNWSNQ GPRGNIDMP 
      NLQALGITG QGQQGSNQQ SCNPLGLGL NLSALPMNP 
      ALVAAALNQ AGWGLIGNL QNQPEPNFN QGFNNGPNN 
      NSGNTPNGS NQGGSSNPG FLSWINQGG GQNQDGGQW 
      PRPPPQQAQ ENKGFLKYD
    CDS sequence
    • >TC006055 RA
      atgtcggtg gagtacctc caagtggcc gaggacgag 
      ggcgaggag cccatcgag ttgcccacc gaagatgac 
      ggaactctg cttctgagc actctggcg gcacagttc 
      ccgggctcc tcggggctc aagtacagg aatcccgag 
      acgaaagcg atgagaggc gtgcgattg agcgagggg 
      cggctgcat ccgccacca gagatcggc tggggcacg 
      cagattttc tactgtgtt tttcccaaa gaaaacaaa 
      cggaaatcg gacgacaat ttagaaaat tcaactgcg 
      aaaacgaaa cgtatggaa acaaaacta agatgtaca 
      gacttgata gtgttagga ttaccttgg aaaacaaca 
      gaacagaat ttacgcgaa tatttcgaa acgttcggt 
      gaagtttta atggctcaa gtgaagaaa gaccccaaa 
      accggccaa tcaaaaggc ttcggtttc attcgcttc 
      gcctcgtac gaatcccag atgcgtgtc ttggcccaa 
      aggcatatg attgacggt cgatggtgt gatgtcaaa 
      gtacccaat tcaaaagaa ggtctgatc caacaagtg 
      ccctgcaaa gtgttcatc ggccgttgc actgaagaa 
      ttaaccgcc gacgatctt cgcgaatat ttcggcaaa 
      tatggcgaa gtcaccgat gttttcatc ccgaaaccg 
      tttcgcgca ttcagtttt gttacattt ctggacccc 
      gaagtggcc caaagtctc tgtggcgag gatcatata 
      atcaagggt gtgtcagtt catgtttca aacgccgcc 
      cccaaatcc gaaacacga ggcggtggt tatgggggc 
      ggcggacca cgaggtgcc aatcatggg aataacagg 
      agcggaccg ggacctgat gggcctggg atgtaccaa 
      caaaggtac aatccaggt ccgaatcag ggcaattgg 
      agcaatcag gggccgagg ggaaatatc gatatgccc 
      aatttacaa gctttaggt ataaccggt caaggtcag 
      caaggtagt aaccaacaa agctgtaat ccattgggt 
      ttgggtttg aatttgagc gctttaccc atgaatccc 
      gctctggtc gctgccgca ttgaatcaa gcaggctgg 
      gggcttatc ggtaattta cagaatcag cccgaaccg 
      aatttcaat cagggcttc aacaacggc cccaacaat 
      aacagcggc aacacgccg aacggcagc aaccagggg 
      ggtagcagc aatcctggt tttctcagt tggataaat 
      cagggcggc ggtcaaaac caagatggc ggccaatgg 
      cctcgaccg cccccccaa caggcgcag gagaacaag 
      ggatttctc aagtacgac taa
    mRNA sequence
    • >TC006055 RA
      gcccgtttg tgtcatctg tcacccgtc attcaaaac 
      cgctaattc gccgatttc gtcccgaaa tcgccccat 
      aacccgtca ttaaatcga aatgggtgc attattgac 
      tcaccgccc ccgttagcc gccccccgg gccgcgttt 
      tgggtcgca tccagtgtg cgagaaacc ggaaatacg 
      tcaaaaaac aaatacgca tctgcatcg gctgccatt 
      ttattttag tgacaagtg cgtgcaagc gtatttaca 
      aatacgctt aaattgtgt gtctgtgtg agggtgcgt 
      gagtgagaa tgtcggtgg agtacctcc aagtggccg 
      aggacgagg gcgaggagc ccatcgagt tgcccaccg 
      aagatgacg gaactctgc ttctgagca ctctggcgg 
      cacagttcc cgggctcct cggggctca agtacagga 
      atcccgaga cgaaagcga tgagaggcg tgcgattga 
      gcgaggggc ggctgcatc cgccaccag agatcggct 
      ggggcacgc agattttct actgtgttt ttcccaaag 
      aaaacaaac ggaaatcgg acgacaatt tagaaaatt 
      caactgcga aaacgaaac gtatggaaa caaaactaa 
      gatgtacag acttgatag tgttaggat taccttgga 
      aaacaacag aacagaatt tacgcgaat atttcgaaa 
      cgttcggtg aagttttaa tggctcaag tgaagaaag 
      accccaaaa ccggccaat caaaaggct tcggtttca 
      ttcgcttcg cctcgtacg aatcccaga tgcgtgtct 
      tggcccaaa ggcatatga ttgacggtc gatggtgtg 
      atgtcaaag tacccaatt caaaagaag gtctgatcc 
      aacaagtgc cctgcaaag tgttcatcg gccgttgca 
      ctgaagaat taaccgccg acgatcttc gcgaatatt 
      tcggcaaat atggcgaag tcaccgatg ttttcatcc 
      cgaaaccgt ttcgcgcat tcagttttg ttacatttc 
      tggaccccg aagtggccc aaagtctct gtggcgagg 
      atcatataa tcaagggtg tgtcagttc atgtttcaa 
      acgccgccc ccaaatccg aaacacgag gcggtggtt 
      atgggggcg gcggaccac gaggtgcca atcatggga 
      ataacagga gcggaccgg gacctgatg ggcctggga 
      tgtaccaac aaaggtaca atccaggtc cgaatcagg 
      gcaattgga gcaatcagg ggccgaggg gaaatatcg 
      atatgccca atttacaag ctttaggta taaccggtc 
      aaggtcagc aaggtagta accaacaaa gctgtaatc 
      cattgggtt tgggtttga atttgagcg ctttaccca 
      tgaatcccg ctctggtcg ctgccgcat tgaatcaag 
      caggctggg ggcttatcg gtaatttac agaatcagc 
      ccgaaccga atttcaatc agggcttca acaacggcc 
      ccaacaata acagcggca acacgccga acggcagca 
      accaggggg gtagcagca atcctggtt ttctcagtt 
      ggataaatc agggcggcg gtcaaaacc aagatggcg 
      gccaatggc ctcgaccgc ccccccaac aggcgcagg 
      agaacaagg gatttctca agtacgact aaatcaaat 
      aattttaaa tccaaccaa tcacaatca ctcctcatc 
      ccttacctt ccttcattc ttatttttc tattacgtt 
      tctctctgc ttacttagt gtgtgtctc tcaaataat 
      ataattcag cacatcatg ttgagaaaa gttttcgga 
      aatgttgtg tgtgaatga cgtcaggtg aagaagaaa 
      attcgagga ctagtcaat tttatacac aaaatatac 
      tcatttgtt ttattatgc cggaagtgt gtgaggtgg 
      ttcttttgt tttgtatat tagctggtt gaagtcgtt 
      tccggaagc tttgctcct actgtagat tagtctagg 
      gaaaaagaa aatgtattg aatttctgt tggtggttt 
      gccggagtt ggcagtttt gattattcc cccacgtgc 
      atcacgacg atcgcatcg taagagttt taaaagata 
      gtgactgta catttactt gtgtttgac tgggttttt 
      aaggataat tatgtaaaa gaggtgata atattatat 
      gagtgcaga tgatctgtg ttttgtttt tatttaatt 
      tgaagacga tactccttg actgtcgta gagacggcg 
      ccacttgca cttgggtca ggtcaggtc aggtcaagt 
      gtgcgccgt cgtagtgtt cctttctcc ctgtgtctt 
      gacagattg catgaagag acttaaaca aaagtgtgg 
      tgaatgagt gtagtaaat ggggacgaa tggtgggac 
      atgtgaaga cgtctgact gagtaagtt tgattcaaa 
      tttttggac aaaatacac tgatgatgg acga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY