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Gene information
General information
TC006048 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2470149..2476176
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006048
    iBeetle screen iB_00986

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006048 RA
      MKFLSLKLL ESLSKCSTR RSPFLNVLT PLIAPTTHT 
      MGCSTSKVE VSRSTAKDE EYVEGVVCQ DSDVGENEM 
      KSFELGEGR VLLVRQKGK LSALGAKCT HYGAPLAAG 
      ALGDGRVRC QWHGACFNI LTGDIEDFP GMDSLPCYQ 
      VTVENNSVK VRAKRSELE TNKRVKSMT KRNPAESQH 
      IVVIGGGPS GAICVETLR QEGFAGQIT LVCKENCLP 
      YDRVLVSKA MDFDLSKAQ FRTEQFYKD HGIDVLKGV 
      KATGVNTTQ KSVNLSNGA VLTYDKLYI ATGMKANKV 
      AIPGADLQN VVVLRNYEH SKATNALIR PDLKVVVLG 
      SSFVALEAA AYCVEKGVK DVTIVARGS VPFKPFLGE 
      VVGGAFKAF FQEKGVKFI SNSGLSKCL DDGTGKVGK 
      VELKDGSTL EADLVIMGV GSSCLTDFL KGSGVELRP 
      DGTVEVNEF LQSNSPNVY VGGDIAYAP VWSHHGHKA 
      AIGHYPLAH YHGKIAALN ILGKGRRLQ AVPYFWTKL 
      FGKSLRYAG HGKFSDVVY IGDVDKFKF VAFYLDGDE 
      VVAAASVGW DPVVSQFAE VLALGKTLT RKDVENEDK 
      FTWTKLLQS T
    CDS sequence
    • >TC006048 RA
      atgaaattc ctttcactc aaactttta gaaagtttg 
      tccaagtgc agcacgcgc cggagcccc tttttaaat 
      gttttaacc ccacttatc gcccccaca acacacacc 
      atgggctgt tccacgtcc aaagttgaa gtttcgcgc 
      tccacggcc aaggatgag gagtacgtg gagggcgtc 
      gtgtgccag gactccgac gtcggcgag aacgagatg 
      aagagcttc gagctgggc gagggccgg gtgctgctg 
      gtgcgccag aagggcaag ctgagcgcg ctcggggcc 
      aagtgcacg cactacggc gcccccctg gccgcgggg 
      gccctcggc gacggccgc gtgcgctgc cagtggcac 
      ggcgcctgc ttcaacatc ctcacaggg gacatcgag 
      gactttcca ggcatggac tcgctccct tgctaccaa 
      gtcaccgtc gagaacaac tcggtcaaa gtccgcgcc 
      aaacgctcc gagctcgag acgaacaaa cgcgtcaaa 
      tccatgaca aaacgcaac ccggctgaa tcgcaacac 
      attgtcgtg atcggcggg gggccctca ggcgccatc 
      tgcgtcgaa accctccgt caggagggt ttcgccggc 
      caaataacc ctcgtctgc aaagaaaac tgtctcccc 
      tacgaccgc gtcctcgtc agcaaagcc atggacttc 
      gacctatcg aaagcccaa ttccgcacc gaacaattc 
      tacaaagac cacggaatc gacgtcctg aaaggcgtc 
      aaagccaca ggagtcaac accacgcaa aaatcggtc 
      aatttaagc aacggcgct gttttaacc tacgacaaa 
      ttgtacatt gccaccggg atgaaggcc aacaaggtg 
      gccattcca ggcgccgat ttgcagaac gtcgtagtc 
      ttgcgaaac tacgagcat tccaaagcc acaaacgcg 
      ctgattcgc cccgatttg aaggttgtt gtcttgggg 
      tcgagcttc gtggcgctt gaagcggcc gcctattgc 
      gtggaaaag ggggtgaaa gacgtgacg atcgtggca 
      cgggggagt gtgcccttt aagcccttc ctgggcgag 
      gtggtgggg ggcgccttc aaggcgttt ttccaggag 
      aaaggcgtc aaattcatc agtaattcc ggtttaagt 
      aaatgtcta gacgatggg accgggaaa gtgggcaaa 
      gtcgaactc aaggacggt tccaccctc gaggccgac 
      ttggtgatc atgggggtt gggagcagt tgtctcacg 
      gatttcctc aagggctcg ggggtggag ctgcgcccc 
      gacggtacg gtcgaagtg aacgagttc ttgcaaagt 
      aacagcccc aatgtgtac gtggggggc gacattgcc 
      tacgccccc gtgtggtcg catcacggc cacaaggcg 
      gcgatcggg cactacccc ctggcccac taccacggg 
      aagatcgca gcgttgaat atcctgggg aaggggcgg 
      aggctgcaa gcggtgcct tatttctgg accaagttg 
      tttgggaaa agtctgagg tatgcaggg catgggaaa 
      ttcagcgat gttgtgtat attggggac gtggataag 
      tttaaattt gtcgcgttt tatctcgat ggggacgag 
      gtggtggcg gcggcgtcg gtgggctgg gaccccgtt 
      gtgtcgcaa tttgccgaa gtcttggcg cttggtaag 
      acgctaaca cgtaaggat gtggaaaat gaggataaa 
      tttacttgg accaaattg ttgcaaagt acttaa
    mRNA sequence
    • >TC006048 RA
      atagcgcac aatgaaatt cctttcact caaactttt 
      agaaagttt gtccaagtg cagcacgcg ccggagccc 
      ctttttaaa tgttttaac cccacttat cgcccccac 
      aacacacac catgggctg ttccacgtc caaagttga 
      agtttcgcg ctccacggc caaggatga ggagtacgt 
      ggagggcgt cgtgtgcca ggactccga cgtcggcga 
      gaacgagat gaagagctt cgagctggg cgagggccg 
      ggtgctgct ggtgcgcca gaagggcaa gctgagcgc 
      gctcggggc caagtgcac gcactacgg cgcccccct 
      ggccgcggg ggccctcgg cgacggccg cgtgcgctg 
      ccagtggca cggcgcctg cttcaacat cctcacagg 
      ggacatcga ggactttcc aggcatgga ctcgctccc 
      ttgctacca agtcaccgt cgagaacaa ctcggtcaa 
      agtccgcgc caaacgctc cgagctcga gacgaacaa 
      acgcgtcaa atccatgac aaaacgcaa cccggctga 
      atcgcaaca cattgtcgt gatcggcgg ggggccctc 
      aggcgccat ctgcgtcga aaccctccg tcaggaggg 
      tttcgccgg ccaaataac cctcgtctg caaagaaaa 
      ctgtctccc ctacgaccg cgtcctcgt cagcaaagc 
      catggactt cgacctatc gaaagccca attccgcac 
      cgaacaatt ctacaaaga ccacggaat cgacgtcct 
      gaaaggcgt caaagccac aggagtcaa caccacgca 
      aaaatcggt caatttaag caacggcgc tgttttaac 
      ctacgacaa attgtacat tgccaccgg gatgaaggc 
      caacaaggt ggccattcc aggcgccga tttgcagaa 
      cgtcgtagt cttgcgaaa ctacgagca ttccaaagc 
      cacaaacgc gctgattcg ccccgattt gaaggttgt 
      tgtcttggg gtcgagctt cgtggcgct tgaagcggc 
      cgcctattg cgtggaaaa gggggtgaa agacgtgac 
      gatcgtggc acgggggag tgtgccctt taagccctt 
      cctgggcga ggtggtggg gggcgcctt caaggcgtt 
      tttccagga gaaaggcgt caaattcat cagtaattc 
      cggtttaag taaatgtct agacgatgg gaccgggaa 
      agtgggcaa agtcgaact caaggacgg ttccaccct 
      cgaggccga cttggtgat catgggggt tgggagcag 
      ttgtctcac ggatttcct caagggctc gggggtgga 
      gctgcgccc cgacggtac ggtcgaagt gaacgagtt 
      cttgcaaag taacagccc caatgtgta cgtgggggg 
      cgacattgc ctacgcccc cgtgtggtc gcatcacgg 
      ccacaaggc ggcgatcgg gcactaccc cctggccca 
      ctaccacgg gaagatcgc agcgttgaa tatcctggg 
      gaaggggcg gaggctgca agcggtgcc ttatttctg 
      gaccaagtt gtttgggaa aagtctgag gtatgcagg 
      gcatgggaa attcagcga tgttgtgta tattgggga 
      cgtggataa gtttaaatt tgtcgcgtt ttatctcga 
      tggggacga ggtggtggc ggcggcgtc ggtgggctg 
      ggaccccgt tgtgtcgca atttgccga agtcttggc 
      gcttggtaa gacgctaac acgtaagga tgtggaaaa 
      tgaggataa atttacttg gaccaaatt gttgcaaag 
      tacttaaac ggggctgtg atcgaagcc acacagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY