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Gene information
General information
TC006047 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 2172813..2178044
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006047
    iBeetle screen iB_00985
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 8 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 90.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 9 (11 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006047 RA
      MLIKEYRVT LPLTVEEYQ VAQLFSVAE ASKDNTGGG 
      EGIEVLKNE PFSNYPLLG GKYTSGQYT YKIYHLASK 
      VPAFIRLLA PRGSLEIHE EAWNAYPYC RTVITNPAY 
      MKEKFRIVI ETLHAPGVG DENNNPGYM KENFVICIE 
      SMHIGDTGN QDNVHELPP DKLKQREVV HIDIANDPI 
      AASDYKEKE DPTKFKSEK TGRGPLVGP NWKNNVQPV 
      MTCYKLVTV EFKWFGLQN RVESFIQKS ERRLFTNFH 
      RQVFCWMDL WHGLTMEDI RAIEDRTQQ ELEALRQKG 
      EVRGMKAET D
    CDS sequence
    • >TC006047 RA
      atgctaatt aaagaatac cgagtgact ttgccccta 
      accgtcgaa gagtaccaa gtcgctcag ttgttcagc 
      gtcgccgag gcttccaaa gacaacacg ggcggcggc 
      gagggcatc gaagtgctg aagaatgag cccttcagc 
      aactacccc ctgctgggg ggcaagtac acctcgggc 
      cagtacacc tacaagatc taccacctg gccagcaaa 
      gtgcccgcc ttcatccgg ctgctggcc cccaggggc 
      agcctcgag atacacgag gaggcctgg aacgcctac 
      ccctactgc cgcaccgtg atcacgaac ccggcgtac 
      atgaaagag aaatttcgt attgttatc gaaactttg 
      catgcaccg ggtgtcgga gacgagaac aataaccct 
      ggatacatg aaagagaac ttcgttatc tgtatcgaa 
      agtatgcac attggggac acgggaaat caagataac 
      gtccacgag cttccgccc gacaagctc aagcagcgg 
      gaggtggtg cacatagac atcgccaac gacccgatc 
      gccgccagc gactacaaa gagaaagag gatccgacg 
      aaattcaag tcggagaag accggtcgg gggcccctg 
      gtgggcccc aattggaaa aacaacgtc cagccggtc 
      atgacctgc tacaaactg gtaaccgtc gagttcaaa 
      tggttcggt ttgcaaaac cgtgtagaa agttttatt 
      caaaagtcg gagcgacgg ctgttcacc aacttccat 
      aggcaagtt ttctgttgg atggacctg tggcacggc 
      ctgaccatg gaggacatc cgggccatc gaggacagg 
      acgcagcaa gaactcgaa gcgctgcgc cagaagggc 
      gaggtgcga ggcatgaaa gcagaaacg gactaa
    mRNA sequence
    • >TC006047 RA
      tcaccaagt tgcgtcatt gcgcctgcg ccgtatgca 
      acccaatta acagcagtc caagtcgag tgaatcaga 
      attacttgt gataacgaa aagctagta ttcacatgt 
      gcacattat ccaaagtga ccgaatcaa gatgctaat 
      taaagaata ccgagtgac tttgcccct aaccgtcga 
      agagtacca agtcgctca gttgttcag cgtcgccga 
      ggcttccaa agacaacac gggcggcgg cgagggcat 
      cgaagtgct gaagaatga gcccttcag caactaccc 
      cctgctggg gggcaagta cacctcggg ccagtacac 
      ctacaagat ctaccacct ggccagcaa agtgcccgc 
      cttcatccg gctgctggc ccccagggg cagcctcga 
      gatacacga ggaggcctg gaacgccta cccctactg 
      ccgcaccgt gatcacgaa cccggcgta catgaaaga 
      gaaatttcg tattgttat cgaaacttt gcatgcacc 
      gggtgtcgg agacgagaa caataaccc tggatacat 
      gaaagagaa cttcgttat ctgtatcga aagtatgca 
      cattgggga cacgggaaa tcaagataa cgtccacga 
      gcttccgcc cgacaagct caagcagcg ggaggtggt 
      gcacataga catcgccaa cgacccgat cgccgccag 
      cgactacaa agagaaaga ggatccgac gaaattcaa 
      gtcggagaa gaccggtcg ggggcccct ggtgggccc 
      caattggaa aaacaacgt ccagccggt catgacctg 
      ctacaaact ggtaaccgt cgagttcaa atggttcgg 
      tttgcaaaa ccgtgtaga aagttttat tcaaaagtc 
      ggagcgacg gctgttcac caacttcca taggcaagt 
      tttctgttg gatggacct gtggcacgg cctgaccat 
      ggaggacat ccgggccat cgaggacag gacgcagca 
      agaactcga agcgctgcg ccagaaggg cgaggtgcg 
      aggcatgaa agcagaaac ggactaaat aaattttgc 
      agttgtagt gaatttaat ttcatttaa acgaactgc 
      ccgcaacgg cacgtagca aattaattt tcgttgttt 
      tattaattg ataagtcta gaaatgcat ttaacattg 
      tgatttggt cgatttgtg gaatagtta ccacgggtt 
      ttgttctta tctagtggt cggtgcagt tcgttgttg 
      ttttaaacc ggtgaaaat aatgaaagc tttcgaaat 
      tttatattt tatttattt attttcatt attttccaa 
      taaactcat tccttttac gacgctccg attttgatt 
      tctctccgg aacgacgaa tctgttgca atattcaga 
      aagccattt tgacgggtt tttctctca tctgtataa 
      atgttatcg agtagttgt taaaattat tgttagaat 
      ttgggatca tgttgacaa tatatctat cttgtataa 
      agtgtcgta gttagcgcc gggcgcctc ggaggaatt 
      tttctatgg caaatgggg cccccggtt cggtcaaaa 
      tggttttag cgaaaaagt caattatga ttgtttagt 
      ccggtccta acgaggcgc aacaactac ttaatttcg 
      acgtagtat taatagtta ggtttttta acgctgatt 
      tactcccgg ctgcagttc catatccat gttggcatt 
      ttaaatgtg tacgtgtaa aattcattt aaattctag 
      tttctagtt caagtatgt atgtaataa tgtgtaaaa 
      agttgtaca caaaacgtt aataaaact agaagtaac 
      ca