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Gene information
General information
TC006041 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2550825..2552328
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006041
    iBeetle screen iB_00984

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: >5 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 70.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006041 RA
      MGTEIDVFI SNYTLVDPE IYQLWVEGH TASEAVSIL 
      NQRGLGQVT GASLELIAS DVLDHYRTY SLLEKLLSN 
      PNKLQEQLA FQIEPQTRQ LLIEKYYDF DDVVVRELI 
      GKKLSSKHR KDLDEVAEK TRVPLKSCR RQFDNVKRI 
      YKMVEDMPG PVVQNIQNH FYLSEPLAK KYACIVFLA 
      CIRFETKRK LNHITFTCW KRCTEAIMD NWTNKLAGV 
      DNFDTELDK DFFTELKDI KIMFEKERE HKQLVCVSL 
      KATLLHKSY TDLDANFKN YNRALLQPA SNMHRPRDV 
      KNMFLELNS LSDSLKQAN WSGQELEIF LNGYTQCGL 
      EIDVIRDSA PMKILWEKY MKVISECLL AMY
    CDS sequence
    • >TC006041 RA
      atgggcacc gaaatcgac gtttttatc agtaattac 
      accctcgtg gaccccgag atttaccag ttatgggtc 
      gagggtcac acagctagc gaagctgtc tcgattctc 
      aaccagagg ggcttggga caggtcact ggtgcctct 
      ctcgagttg atcgcaagc gatgtttta gaccactac 
      cgtacttac tctcttctg gaaaaacta ctcagtaac 
      cccaataaa ttacaggag caattggcg ttccagatt 
      gagcctcag actcggcaa ttattgatc gagaaatac 
      tacgatttt gacgatgtg gtcgtccgg gaactgata 
      gggaaaaaa ttgtcaagt aaacacagg aaggattta 
      gacgaagtg gccgaaaag acaagagtg cccctcaag 
      tcgtgtcgg cgccaattc gataatgtc aaacgtatt 
      tacaagatg gtggaggat atgccgggc cccgttgtg 
      caaaacata caaaatcac ttctacttg agtgaacct 
      ttggccaaa aaatacgcg tgtattgtc tttcttgct 
      tgtattcgc tttgagact aaacggaag ctcaaccac 
      attacattc acgtgttgg aaacgctgc actgaggca 
      atcatggac aactggacg aataaattg gccggggtt 
      gataacttt gatacagaa ctcgacaaa gacttcttc 
      actgaattg aaagacatc aaaatcatg tttgagaag 
      gaacgagaa cacaaacag ttggtgtgc gtgtccctc 
      aaggccaca ctattgcac aagtcttac accgacttg 
      gacgcaaat tttaaaaac tacaatcgc gcactttta 
      caacccgcc agtaacatg cataggccc agagacgtc 
      aaaaacatg ttcctcgaa ttaaactca ctctcagat 
      tctctaaaa caagccaat tggagcgga caagaattg 
      gaaattttc ctcaacggt tatacgcaa tgtggactc 
      gaaattgac gttataagg gactcggcc cccatgaag 
      atactttgg gagaaatac atgaaagtc atttcagaa 
      tgtctccta gctatgtac taa
    mRNA sequence
    • >TC006041 RA
      ttcggtttc ttgcgcaaa aagagcagt tggcgccac 
      aatcggcga tcaattttt ggcaaaaat gagtttatt 
      ttagcccca tgggcaccg aaatcgacg tttttatca 
      gtaattaca ccctcgtgg accccgaga tttaccagt 
      tatgggtcg agggtcaca cagctagcg aagctgtct 
      cgattctca accagaggg gcttgggac aggtcactg 
      gtgcctctc tcgagttga tcgcaagcg atgttttag 
      accactacc gtacttact ctcttctgg aaaaactac 
      tcagtaacc ccaataaat tacaggagc aattggcgt 
      tccagattg agcctcaga ctcggcaat tattgatcg 
      agaaatact acgattttg acgatgtgg tcgtccggg 
      aactgatag ggaaaaaat tgtcaagta aacacagga 
      aggatttag acgaagtgg ccgaaaaga caagagtgc 
      ccctcaagt cgtgtcggc gccaattcg ataatgtca 
      aacgtattt acaagatgg tggaggata tgccgggcc 
      ccgttgtgc aaaacatac aaaatcact tctacttga 
      gtgaacctt tggccaaaa aatacgcgt gtattgtct 
      ttcttgctt gtattcgct ttgagacta aacggaagc 
      tcaaccaca ttacattca cgtgttgga aacgctgca 
      ctgaggcaa tcatggaca actggacga ataaattgg 
      ccggggttg ataactttg atacagaac tcgacaaag 
      acttcttca ctgaattga aagacatca aaatcatgt 
      ttgagaagg aacgagaac acaaacagt tggtgtgcg 
      tgtccctca aggccacac tattgcaca agtcttaca 
      ccgacttgg acgcaaatt ttaaaaact acaatcgcg 
      cacttttac aacccgcca gtaacatgc ataggccca 
      gagacgtca aaaacatgt tcctcgaat taaactcac 
      tctcagatt ctctaaaac aagccaatt ggagcggac 
      aagaattgg aaattttcc tcaacggtt atacgcaat 
      gtggactcg aaattgacg ttataaggg actcggccc 
      ccatgaaga tactttggg agaaataca tgaaagtca 
      tttcagaat gtctcctag ctatgtact aatactact 
      aattttata gtatgtatg tcttcatct gtttcgcag 
      atgccactt gttagagtt ttattaata ttttgggag 
      accctttgt gcaatcact cacttctcc cttccaaaa 
      tcaaaaaaa aatcagatg taaatttgc gtcagtccc 
      cagtactga acacattgt atactataa aaaaatgta 
      actttgtgg tttaataaa cttacattt ctaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY