General information | |||
TC006034 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 2621995..2629352 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs | TC006034 |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00982 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)
- >TC006034 RA
MKCIVLLLL LAQAKTQLI SPCPRLFQY EPQGSENDR WYATVTLIS DAELSGVWL RLIFDKPSI QLGNWFGEV VTTDNKEYL IKNRNHKLA ANTPYKLRF YLKYNPGEK PPQLVMFRL NARLACPEN GVTTEAPVT TGQLITSSL LSTTRNPLA NSNSNNNNN NNQGTTFVK PGGAFNQQT TNSGDDDDF FHGDFAIFH RPKPSLDAG AACGTVVMP PRPLITHGQ ATHEGEFPW HAALYHATG IDLTYICGA SLITRYHLL TVAHCVTKP KSQEKLDPG SLVVYLGKY YLKRWSNPG IQDKHVDKI TVHPDYKSQ VFTNDIAIL RMASPVELT NYVRPVCLW DENTQLQAV INKAGTVVG WGFDENGRV TEQLTKAKM PVVSQETCI YSFPDFYSR FTSDKTYCA GFKNGTSVC NGDSGGGMV FPKSNSNLN NPTWQLRGL VSISVALQN QLKCDASHY VVFTDVAKY LDWIKASLN
- >TC006034 RA
atgaagtgt atcgtcttg ttgctgctc ctggcccaa gctaaaacc cagcttatc tccccctgc cccagactg ttccaatac gagccccag ggatcagaa aacgaccga tggtacgcc actgtcaca ctcataagc gatgcggaa ttatcaggg gtttggttg cgcttaatt ttcgacaaa ccctcaatt cagctcgga aactggttc ggcgaagtt gtgacaaca gacaacaag gaatattta atcaaaaat cgcaaccac aaactggcc gccaacaca ccgtacaaa ctccgattt tacctcaaa tacaacccg ggggaaaaa ccgccacag ttggtcatg tttcggcta aatgcgcgg ttggcgtgc cccgaaaat ggggtcacc accgaagcc cccgtcacg acgggacaa ctcattaca agtagtttg ctttcaacg acacgtaac ccgttggcg aatagtaac agtaacaat aataataac aacaatcaa gggactact ttcgtcaaa ccaggaggg gcttttaac caacaaacg acaaattcg ggcgatgat gacgatttt ttccacggg gattttgcc atttttcat cgcccgaaa ccctcactg gacgcgggg gctgcttgt ggcaccgtt gttatgccc ccacgtccg ctcataacc cacggacag gccacccac gagggcgag ttcccctgg catgcagcc ctctaccac gccactggc atcgacctg acctacatc tgtggggcc tcccttatc acccgttat cacctctta accgtagcc cactgtgtc accaaacct aaatctcag gaaaaactc gatcctggc agtttagta gtttattta gggaaatac tatttgaaa agatggtca aatccaggg attcaagac aaacacgtc gacaaaatc acagtccat cccgattac aaatcgcaa gttttcaca aacgatatt gcaatcctt cggatggct tcgcctgta gaactcact aattatgtc cgaccggtg tgtctttgg gacgaaaat acgcaattg caggctgtt attaacaaa gctggaacg gtggtgggc tggggtttt gacgaaaac ggccgagtg accgagcag ctgaccaag gccaaaatg ccggtcgtg tcgcaagaa acgtgcatt tactcgttc cctgatttc tactcgcgt ttcacttcg gacaaaacc tactgtgct ggatttaaa aatggtact tcagtgtgc aatggggac tcggggggc ggtatggtg ttccccaaa tcaaactcg aatttgaat aatccgacg tggcaattg aggggcctt gtctcgata agtgtggcc ttgcaaaac caactcaaa tgtgatgcc tcgcattat gttgttttc acagatgtg gcgaagtat ctcgactgg attaaagcc tcgcttaat taa
- >TC006034 RA
attggtgcg cttggattt ttgaaaatt gttaaaata aaacttttt ttctttaac attccttgt ttgtcttcc aaacccaca aataacaca taaatattt aaattgcgg cgccaaatt caaaaagaa gacgattta actgtactt taagtgacc gttattgac ccatgacca tcaccaacg gggattcct accttgtcc ttgactgca accattaaa ttcacgcaa aacaggttt caagatttt accactgtc ataacaaac gcagataag atccaaact taagccctg atgctcctc gtgcctccc agaaaatga acgtagtga ttaaacgat aaaaaacgg tgtgttttt agtgttggc tatcgtcca gaaagtaag ttttttcaa tcagcaaaa cgccttgat atttaccaa acgagttat tttcgaaaa aacactgca ccaagtaag tcgaagaac aaaaaacga atttcaaaa gaatgaata atcgctaaa aatatttac tacaaaaac acgcaaagg aatccgcca acgttacca tgaagtgta tcgtcttgt tgctgctcc tggcccaag ctaaaaccc agcttatct ccccctgcc ccagactgt tccaatacg agccccagg gatcagaaa acgaccgat ggtacgcca ctgtcacac tcataagcg atgcggaat tatcagggg tttggttgc gcttaattt tcgacaaac cctcaattc agctcggaa actggttcg gcgaagttg tgacaacag acaacaagg aatatttaa tcaaaaatc gcaaccaca aactggccg ccaacacac cgtacaaac tccgatttt acctcaaat acaacccgg gggaaaaac cgccacagt tggtcatgt ttcggctaa atgcgcggt tggcgtgcc ccgaaaatg gggtcacca ccgaagccc ccgtcacga cgggacaac tcattacaa gtagtttgc tttcaacga cacgtaacc cgttggcga atagtaaca gtaacaata ataataaca acaatcaag ggactactt tcgtcaaac caggagggg cttttaacc aacaaacga caaattcgg gcgatgatg acgattttt tccacgggg attttgcca tttttcatc gcccgaaac cctcactgg acgcggggg ctgcttgtg gcaccgttg ttatgcccc cacgtccgc tcataaccc acggacagg ccacccacg agggcgagt tcccctggc atgcagccc tctaccacg ccactggca tcgacctga cctacatct gtggggcct cccttatca cccgttatc acctcttaa ccgtagccc actgtgtca ccaaaccta aatctcagg aaaaactcg atcctggca gtttagtag tttatttag ggaaatact atttgaaaa gatggtcaa atccaggga ttcaagaca aacacgtcg acaaaatca cagtccatc ccgattaca aatcgcaag ttttcacaa acgatattg caatccttc ggatggctt cgcctgtag aactcacta attatgtcc gaccggtgt gtctttggg acgaaaata cgcaattgc aggctgtta ttaacaaag ctggaacgg tggtgggct ggggttttg acgaaaacg gccgagtga ccgagcagc tgaccaagg ccaaaatgc cggtcgtgt cgcaagaaa cgtgcattt actcgttcc ctgatttct actcgcgtt tcacttcgg acaaaacct actgtgctg gatttaaaa atggtactt cagtgtgca atggggact cggggggcg gtatggtgt tccccaaat caaactcga atttgaata atccgacgt ggcaattga ggggccttg tctcgataa gtgtggcct tgcaaaacc aactcaaat gtgatgcct cgcattatg ttgttttca cagatgtgg cgaagtatc tcgactgga ttaaagcct cgcttaatt aaatgaatt atgttttta tatggtgat aaataaaca c
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
