Status
Please wait ...
Gene information
General information
TC006034 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2621995..2629352
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006034
    iBeetle screen iB_00982

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 40.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006034 RA
      MKCIVLLLL LAQAKTQLI SPCPRLFQY EPQGSENDR 
      WYATVTLIS DAELSGVWL RLIFDKPSI QLGNWFGEV 
      VTTDNKEYL IKNRNHKLA ANTPYKLRF YLKYNPGEK 
      PPQLVMFRL NARLACPEN GVTTEAPVT TGQLITSSL 
      LSTTRNPLA NSNSNNNNN NNQGTTFVK PGGAFNQQT 
      TNSGDDDDF FHGDFAIFH RPKPSLDAG AACGTVVMP 
      PRPLITHGQ ATHEGEFPW HAALYHATG IDLTYICGA 
      SLITRYHLL TVAHCVTKP KSQEKLDPG SLVVYLGKY 
      YLKRWSNPG IQDKHVDKI TVHPDYKSQ VFTNDIAIL 
      RMASPVELT NYVRPVCLW DENTQLQAV INKAGTVVG 
      WGFDENGRV TEQLTKAKM PVVSQETCI YSFPDFYSR 
      FTSDKTYCA GFKNGTSVC NGDSGGGMV FPKSNSNLN 
      NPTWQLRGL VSISVALQN QLKCDASHY VVFTDVAKY 
      LDWIKASLN
    CDS sequence
    • >TC006034 RA
      atgaagtgt atcgtcttg ttgctgctc ctggcccaa 
      gctaaaacc cagcttatc tccccctgc cccagactg 
      ttccaatac gagccccag ggatcagaa aacgaccga 
      tggtacgcc actgtcaca ctcataagc gatgcggaa 
      ttatcaggg gtttggttg cgcttaatt ttcgacaaa 
      ccctcaatt cagctcgga aactggttc ggcgaagtt 
      gtgacaaca gacaacaag gaatattta atcaaaaat 
      cgcaaccac aaactggcc gccaacaca ccgtacaaa 
      ctccgattt tacctcaaa tacaacccg ggggaaaaa 
      ccgccacag ttggtcatg tttcggcta aatgcgcgg 
      ttggcgtgc cccgaaaat ggggtcacc accgaagcc 
      cccgtcacg acgggacaa ctcattaca agtagtttg 
      ctttcaacg acacgtaac ccgttggcg aatagtaac 
      agtaacaat aataataac aacaatcaa gggactact 
      ttcgtcaaa ccaggaggg gcttttaac caacaaacg 
      acaaattcg ggcgatgat gacgatttt ttccacggg 
      gattttgcc atttttcat cgcccgaaa ccctcactg 
      gacgcgggg gctgcttgt ggcaccgtt gttatgccc 
      ccacgtccg ctcataacc cacggacag gccacccac 
      gagggcgag ttcccctgg catgcagcc ctctaccac 
      gccactggc atcgacctg acctacatc tgtggggcc 
      tcccttatc acccgttat cacctctta accgtagcc 
      cactgtgtc accaaacct aaatctcag gaaaaactc 
      gatcctggc agtttagta gtttattta gggaaatac 
      tatttgaaa agatggtca aatccaggg attcaagac 
      aaacacgtc gacaaaatc acagtccat cccgattac 
      aaatcgcaa gttttcaca aacgatatt gcaatcctt 
      cggatggct tcgcctgta gaactcact aattatgtc 
      cgaccggtg tgtctttgg gacgaaaat acgcaattg 
      caggctgtt attaacaaa gctggaacg gtggtgggc 
      tggggtttt gacgaaaac ggccgagtg accgagcag 
      ctgaccaag gccaaaatg ccggtcgtg tcgcaagaa 
      acgtgcatt tactcgttc cctgatttc tactcgcgt 
      ttcacttcg gacaaaacc tactgtgct ggatttaaa 
      aatggtact tcagtgtgc aatggggac tcggggggc 
      ggtatggtg ttccccaaa tcaaactcg aatttgaat 
      aatccgacg tggcaattg aggggcctt gtctcgata 
      agtgtggcc ttgcaaaac caactcaaa tgtgatgcc 
      tcgcattat gttgttttc acagatgtg gcgaagtat 
      ctcgactgg attaaagcc tcgcttaat taa
    mRNA sequence
    • >TC006034 RA
      attggtgcg cttggattt ttgaaaatt gttaaaata 
      aaacttttt ttctttaac attccttgt ttgtcttcc 
      aaacccaca aataacaca taaatattt aaattgcgg 
      cgccaaatt caaaaagaa gacgattta actgtactt 
      taagtgacc gttattgac ccatgacca tcaccaacg 
      gggattcct accttgtcc ttgactgca accattaaa 
      ttcacgcaa aacaggttt caagatttt accactgtc 
      ataacaaac gcagataag atccaaact taagccctg 
      atgctcctc gtgcctccc agaaaatga acgtagtga 
      ttaaacgat aaaaaacgg tgtgttttt agtgttggc 
      tatcgtcca gaaagtaag ttttttcaa tcagcaaaa 
      cgccttgat atttaccaa acgagttat tttcgaaaa 
      aacactgca ccaagtaag tcgaagaac aaaaaacga 
      atttcaaaa gaatgaata atcgctaaa aatatttac 
      tacaaaaac acgcaaagg aatccgcca acgttacca 
      tgaagtgta tcgtcttgt tgctgctcc tggcccaag 
      ctaaaaccc agcttatct ccccctgcc ccagactgt 
      tccaatacg agccccagg gatcagaaa acgaccgat 
      ggtacgcca ctgtcacac tcataagcg atgcggaat 
      tatcagggg tttggttgc gcttaattt tcgacaaac 
      cctcaattc agctcggaa actggttcg gcgaagttg 
      tgacaacag acaacaagg aatatttaa tcaaaaatc 
      gcaaccaca aactggccg ccaacacac cgtacaaac 
      tccgatttt acctcaaat acaacccgg gggaaaaac 
      cgccacagt tggtcatgt ttcggctaa atgcgcggt 
      tggcgtgcc ccgaaaatg gggtcacca ccgaagccc 
      ccgtcacga cgggacaac tcattacaa gtagtttgc 
      tttcaacga cacgtaacc cgttggcga atagtaaca 
      gtaacaata ataataaca acaatcaag ggactactt 
      tcgtcaaac caggagggg cttttaacc aacaaacga 
      caaattcgg gcgatgatg acgattttt tccacgggg 
      attttgcca tttttcatc gcccgaaac cctcactgg 
      acgcggggg ctgcttgtg gcaccgttg ttatgcccc 
      cacgtccgc tcataaccc acggacagg ccacccacg 
      agggcgagt tcccctggc atgcagccc tctaccacg 
      ccactggca tcgacctga cctacatct gtggggcct 
      cccttatca cccgttatc acctcttaa ccgtagccc 
      actgtgtca ccaaaccta aatctcagg aaaaactcg 
      atcctggca gtttagtag tttatttag ggaaatact 
      atttgaaaa gatggtcaa atccaggga ttcaagaca 
      aacacgtcg acaaaatca cagtccatc ccgattaca 
      aatcgcaag ttttcacaa acgatattg caatccttc 
      ggatggctt cgcctgtag aactcacta attatgtcc 
      gaccggtgt gtctttggg acgaaaata cgcaattgc 
      aggctgtta ttaacaaag ctggaacgg tggtgggct 
      ggggttttg acgaaaacg gccgagtga ccgagcagc 
      tgaccaagg ccaaaatgc cggtcgtgt cgcaagaaa 
      cgtgcattt actcgttcc ctgatttct actcgcgtt 
      tcacttcgg acaaaacct actgtgctg gatttaaaa 
      atggtactt cagtgtgca atggggact cggggggcg 
      gtatggtgt tccccaaat caaactcga atttgaata 
      atccgacgt ggcaattga ggggccttg tctcgataa 
      gtgtggcct tgcaaaacc aactcaaat gtgatgcct 
      cgcattatg ttgttttca cagatgtgg cgaagtatc 
      tcgactgga ttaaagcct cgcttaatt aaatgaatt 
      atgttttta tatggtgat aaataaaca c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY