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Gene information
General information
TC033958
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006030,TC006031
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 2379764..2386141
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033958
    iBeetle screen iB_00981
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 50.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033958 RA
      MPRSRYPFD VENMEEEGD FGGEVSVVD VYDIASDIG 
      KECEKIIDQ YGADAVTSL MPKVINALE LLENLATRN 
      ERENTQLHE LRLKIAQLE NDKLEKAEY RQKFEKELE 
      AIEEQWRSE TKELLSVVT RLQEENRRL AKDNSTSPH 
      RQVPPLSPP PNGDMLQKL KDSVEKQRD EIRLKDNLL 
      QEKCHDIEN LKSQVERLN NTSRELRRK HKLFQSQVR 
      TLCDERADF LVQLQDQQR DIVGLRQRL GLAQKENED 
      LAKCDPPVP VNKAVYDLD DPNRPRFTT QELKDILHE 
      RNELKARVS DLEDELETY RPKNKSESP VEEDAPVQG 
      PLPYEPDDA PWKKCSESG IRKFFRKIF SESSSGSSF 
      PRRSLSTLS KMALSATNE PVPV
    CDS sequence
    • >TC033958 RA
      atgcctcga tcccggtac cccttcgac gttgaaaat 
      atggaagaa gagggtgat ttcggtggg gaagtcagt 
      gttgtcgat gtctatgat attgcttcc gatattggc 
      aaagaatgc gaaaaaatc attgatcaa tacggtgct 
      gatgctgtg acttcgtta atgcccaaa gttattaac 
      gccttagaa ttgctcgaa aatctggcc acacgcaac 
      gaacgcgaa aacacacaa ttgcatgag cttcgactg 
      aaaatagca caattggaa aatgacaag ttggaaaag 
      gcggaatat cggcaaaaa tttgaaaaa gaactggaa 
      gccattgaa gaacaatgg cgttctgaa accaaagaa 
      ttgctttct gtggtcaca cgtctccag gaggaaaac 
      cggcgattg gccaaagac aactccacg tctccccat 
      cgacaggtg ccccccttg tcgccgccg cctaacggc 
      gacatgtta cagaagctg aaagacagt gtggagaaa 
      caacgcgat gaaatccgc ctcaaagac aatctccta 
      caagagaaa tgccacgat attgaaaat ttgaaatcg 
      caagttgaa cgtctgaac aacacgagt cgagagctt 
      cgccgcaaa cataagtta ttccaaagc caagtgcgc 
      acgttatgc gacgaaagg gccgatttt ctcgtccaa 
      cttcaagac caacaacga gatatcgtc gggttgagg 
      caaagattg ggtttggcg cagaaggaa aacgaagat 
      cttgcaaaa tgtgatccc ccggttccg gttaacaaa 
      gccgtctat gatttggac gatccgaac cggccgagg 
      ttcactact caggagctt aaagacata ttacacgaa 
      cggaacgaa ctcaaagcg cgtgttagt gacttggag 
      gacgaactc gagacgtac agaccgaaa aacaagtcg 
      gaaagtccg gttgaggag gatgcgcct gttcaaggg 
      cctctgccg tatgagccc gacgacgcc ccctggaag 
      aagtgttca gagtcgggg attaggaaa ttttttcgc 
      aagatcttt tccgaaagc agcagtggg agtagtttt 
      cctcggcgt agcttatcg accttgtcg aagatggct 
      ttatcggct actaacgaa ccagttcca gtttaa
    mRNA sequence
    • >TC033958 RA
      gatcaataa ggtgtagtg tgaggagtt tttgcaaaa 
      agttttgat gcctcgatc ccggtaccc cttcgacgt 
      tgaaaatat ggaagaaga gggtgattt cggtgggga 
      agtcagtgt tgtcgatgt ctatgatat tgcttccga 
      tattggcaa agaatgcga aaaaatcat tgatcaata 
      cggtgctga tgctgtgac ttcgttaat gcccaaagt 
      tattaacgc cttagaatt gctcgaaaa tctggccac 
      acgcaacga acgcgaaaa cacacaatt gcatgagct 
      tcgactgaa aatagcaca attggaaaa tgacaagtt 
      ggaaaaggc ggaatatcg gcaaaaatt tgaaaaaga 
      actggaagc cattgaaga acaatggcg ttctgaaac 
      caaagaatt gctttctgt ggtcacacg tctccagga 
      ggaaaaccg gcgattggc caaagacaa ctccacgtc 
      tccccatcg acaggtgcc ccccttgtc gccgccgcc 
      taacggcga catgttaca gaagctgaa agacagtgt 
      ggagaaaca acgcgatga aatccgcct caaagacaa 
      tctcctaca agagaaatg ccacgatat tgaaaattt 
      gaaatcgca agttgaacg tctgaacaa cacgagtcg 
      agagcttcg ccgcaaaca taagttatt ccaaagcca 
      agtgcgcac gttatgcga cgaaagggc cgattttct 
      cgtccaact tcaagacca acaacgaga tatcgtcgg 
      gttgaggca aagattggg tttggcgca gaaggaaaa 
      cgaagatct tgcaaaatg tgatccccc ggttccggt 
      taacaaagc cgtctatga tttggacga tccgaaccg 
      gccgaggtt cactactca ggagcttaa agacatatt 
      acacgaacg gaacgaact caaagcgcg tgttagtga 
      cttggagga cgaactcga gacgtacag accgaaaaa 
      caagtcgga aagtccggt tgaggagga tgcgcctgt 
      tcaagggcc tctgccgta tgagcccga cgacgcccc 
      ctggaagaa gtgttcaga gtcggggat taggaaatt 
      ttttcgcaa gatcttttc cgaaagcag cagtgggag 
      tagttttcc tcggcgtag cttatcgac cttgtcgaa 
      gatggcttt atcggctac taacgaacc agttccagt 
      ttaacgacg atttttttc caaaattcg aaattgatt 
      tggctggta acgtgcagc actcattgt gatagtttt 
      agaggtttt tttttaacc gagaaatta ttgtggacc 
      acttttttt ttactagtc aattgcgaa tatttgcac 
      atatcttat gcttccagt ttgatttcc gacagtatt 
      ttagcgcgt agaaaagaa ccaagcgaa ttgtggtat 
      tagggtccc ctccctatt tttttactt tttttatta 
      tcgtaaagc agtgttggc gaacctgca ggaaattaa 
      gaaaaaata aaattctag tttaagttc gaaacaccg 
      ttccctcca tatcgaatg acactgaat cataaaact 
      gtaaggtat tttttttaa tcgagtatt tccccgaac 
      tagctttct tcgttgtgc aactgttaa agaagaaac 
      ttttttgcg tttcttttt gtgcgaaat ctaacgaga 
      aactggatt gtttcacac aatttcctc aaaaataat 
      tcgaaaaat cattattca attatttaa aaaatagcc 
      tgatttgtt tttaaagtg attaatttg ggtcgaaat 
      ttctttaaa accgtctaa aaaatcgcc aaaataagc 
      cgaaaataa catttttat cgttttaca accagcaac 
      acatccgag aaatcatga aatttggtt caaacgtcg 
      ttattcttg ctttttttg agctaaaaa ctcagtttt 
      ttttggaag attttatga aatttttcg aaatttttt 
      ttaaaaact ggtgcaaat tcgcctcta ggtaaattt 
      aattttttt ggcttgttt tactcgatt ttttgtttg 
      atttttaac tagaggaca cgtgtaaga cac