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Gene information
General information
TC006025 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2843039..2847843
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC006025
    iBeetle screen iB_00979

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%
    larval musculature irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection

    Fertility
    egg production: number no/almost no eggs -
    Comment: No phenotype found!
    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006025 RA
      MSKKAIADQ EITIKDSLF EFVKTHITD ADLSLIDDI 
VLSYVVAIL EDVSSDPVF NVEDFCEMM TAYFPEFES 
INHATVCQW IFELAAKLR VEEPEEKSV IEISLPEIV 
PKTKPRQLD NRDSFDLPK RVHRLSEMS DGGSTDSSC 
CDFLDEIDV LQEMFPHVC SIEVKHCLA IASGDIERA 
TQILIDRQE NGQCLSQNN SLTIHVNKT TIDDAELKN 
RIIERYSYI DKDAFTKEH RPVAPKVEP KKLVRYRDN 
KIVSLKGER FTEVKKGED VDDISLKKN KKGHSTP
    CDS sequence
    • >TC006025 RA
      atgtccaaa aaggccatc gccgatcag gaaatcacc 
atcaaagac tcccttttc gagttcgtt aagacccac 
ataacggac gccgacttg tccctcatc gacgatatc 
gtcctgtct tatgtcgtt gcgatcctg gaggacgtc 
agttcggac cctgtcttc aacgttgaa gatttctgc 
gaaatgatg actgcgtac ttccccgaa ttcgagtcg 
atcaatcat gcgactgtc tgtcagtgg atttttgaa 
ttggcggcg aagttgagg gttgaggaa ccggaagag 
aagtccgtg attgagatt tcgttaccg gaaattgta 
ccgaagaca aaaccgaga caattggat aacagggat 
agctttgat ttgcccaaa agagtgcac aggttgtcg 
gaaatgagc gatgggggc tccacggac agctcctgc 
tgcgatttt ttggacgaa atcgatgtc ttgcaagaa 
atgttcccc cacgtttgc tccattgag gtgaaacat 
tgcttggcc atcgccagc ggggacata gagcgcgcc 
acccagatt ctgatcgat cgtcaagaa aacggccaa 
tgcctcagc cagaacaat tcgttgacg atccacgtc 
aacaagaca acaatcgac gacgcagaa ttgaagaac 
cgcatcatc gagcggtac tcctacatc gacaaggac 
gccttcacc aaggagcac cggccggtg gcccccaaa 
gtggagccc aagaagctg gtccgttac cgcgacaac 
aaaatcgta agtttgaag ggcgagcgc ttcaccgaa 
gtgaagaag ggcgaggac gtcgacgac atctcgctg 
aagaagaac aagaagggc cacagcacg ccgtaa
    mRNA sequence
    • >TC006025 RA
      caggaggat taaacagga atagggttt ttcgccgct 
tgacattcg ccaccagtg gcgccccct actccgtca 
cttttgttg tgttattga ttgaatcga gtaaatcaa 
aaatatccg gttttgtgg caaaacggg cacaagtcg 
ggtcttcgc cgccaagtc cgaggtaca aaaggtcga 
gtttcattg ataagcccc caaaccagc cctcagaac 
gccacttta acccccaac tcgtttccc tgattccgg 
ggcgaattt cactcattt cccgtcgaa aatgtccaa 
aaaggccat cgccgatca ggaaatcac catcaaaga 
ctccctttt cgagttcgt taagaccca cataacgga 
cgccgactt gtccctcat cgacgatat cgtcctgtc 
ttatgtcgt tgcgatcct ggaggacgt cagttcgga 
ccctgtctt caacgttga agatttctg cgaaatgat 
gactgcgta cttccccga attcgagtc gatcaatca 
tgcgactgt ctgtcagtg gatttttga attggcggc 
gaagttgag ggttgagga accggaaga gaagtccgt 
gattgagat ttcgttacc ggaaattgt accgaagac 
aaaaccgag acaattgga taacaggga tagctttga 
tttgcccaa aagagtgca caggttgtc ggaaatgag 
cgatggggg ctccacgga cagctcctg ctgcgattt 
tttggacga aatcgatgt cttgcaaga aatgttccc 
ccacgtttg ctccattga ggtgaaaca ttgcttggc 
catcgccag cggggacat agagcgcgc cacccagat 
tctgatcga tcgtcaaga aaacggcca atgcctcag 
ccagaacaa ttcgttgac gatccacgt caacaagac 
aacaatcga cgacgcaga attgaagaa ccgcatcat 
cgagcggta ctcctacat cgacaagga cgccttcac 
caaggagca ccggccggt ggcccccaa agtggagcc 
caagaagct ggtccgtta ccgcgacaa caaaatcgt 
aagtttgaa gggcgagcg cttcaccga agtgaagaa 
gggcgagga cgtcgacga catctcgct gaagaagaa 
caagaaggg ccacagcac gccgtaacc aacaaccga 
cgttcggat gaataaaaa tagttttaa aattctgat 
tagagtagg gtatattta tgtaatgtc tggcactct 
taactagct atagggagc aagtgttgg gtaggggat 
agcgtccgg aacccaaca acttgctcc tcgtctaca 
cacaccggt agtattatt attataagg ttcaagact 
agtcaaaat cgcggaggt ggctcgtgc gtatcctac 
ttcatttcg aatacctgt gattctcgt aaagtaaat 
gtattttcg tacatttta tctcaatca gtgatatta 
tcaagcatt tggttgctt cggttttaa cgttcaaga 
cttgtgttt tgtgttgtt tatcccttt tttaccttt 
ctcgattat aataaatat gttaagtgt aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY