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Gene information
General information
TC006024 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2850645..2869212
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006024
    iBeetle screen iB_00978

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head partially shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: A dent near the labrum
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006024 RA
      MAFLRSLSR SSASRSYGL LVELSRLRL AAAKSTAAV 
      VQDAAAAPV AGKRRDPLD ITFEDAKAA FKSKTNWEL 
      MRAYIVYTL CSFETLVEN NMQLMKLCK GILGERLFI 
      MLMRATFYG HFVAGEDQY KIRPTLERL RSFGVKPIL 
      DYSVEEDLS QEEAEKREV EASVPEAGD QVEQGSLKQ 
      YHVDKTFAD RRYKVQSAR TYFYLNEAT CERNMETFI 
      KCLEAVADF QKNPSGATY GTGITAIKL TALGRPQLL 
      LQLSEVIMK ARKYMTEIV GGEGNVLSH HMTIQDLER 
      KLSKMKDQE SVKKFLSQI TADEKGILH LFPWSGIID 
      ENQELSSTF RVPDLTTGR MSRLITQLS PKEEEMFRN 
      MIRRMNTIV KAAQDMDVR IMVDAEQTY FQPAITRIT 
      LEFMRKYNK DKAIVFNTY QCYLRNAFD EVTTDMEQA 
      KRQNFYFGA KLVRGAYLE QERARAAAL GYPDPTNPN 
      FEATSDMYH KTLTECLRR IKAFKDLGE EKKIAIMVA 
      SHNEDTVRF AIQKMKEFG IEPEDKVIC FGQLLAMCD 
      YITFPLGQS GYSAYKYIP YGPVNEVLP YLSRRAHEN 
      KGVLKKIKK EKRLLGSEI LRRVATGQW WYTPKGDYT 
      PV
    CDS sequence
    • >TC006024 RA
      atggctttt ctgcgttcg ctgtcccgg agcagcgcg 
      tccaggtcg tacggcctg ctggtggag ctctcccgc 
      ctccgtctc gccgccgcc aagtcgacg gccgccgtg 
      gtgcaggac gccgccgcc gcccccgtc gccggcaag 
      cggcgcgac cccctcgac atcaccttc gaggacgcc 
      aaggccgcc ttcaagagc aagaccaac tgggagctg 
      atgcgcgcc tacatcgtc tacacgctc tgcagcttc 
      gagaccctc gtcgagaac aacatgcag ctgatgaaa 
      ctatgtaaa ggtatccta ggagaaaga ctgtttata 
      atgctgatg cgagcaacg ttctacggg cacttcgtt 
      gccggcgaa gatcaatac aaaatacga ccgactttg 
      gagcgattg cgatcgttc ggcgtcaag ccgatcctc 
      gactactcg gtcgaagag gacctatcg caagaagag 
      gccgaaaaa cgcgaagtc gaagcttcg gttcccgaa 
      gccggcgat caggtcgaa cagggttcg ttgaaacag 
      taccacgtg gataaaaca ttcgccgat cgacgttat 
      aaggtccaa tcagcacgc acgtacttc tacttgaac 
      gaagcgacc tgcgaacgc aacatggag accttcatc 
      aagtgcttg gaggccgtc gcggacttt cagaagaat 
      ccgtcaggt gccacatac ggcactggc atcaccgcc 
      attaaatta acggccctt ggcagacct caactattg 
      cttcaatta tcggaagta atcatgaaa gctcgtaaa 
      tacatgacg gaaatcgtg ggcggcgaa ggcaacgtc 
      ctctcccat cacatgacc atccaagat ctcgaacgt 
      aaactttcg aaaatgaaa gatcaagaa tcggtgaaa 
      aagttcctg tcgcaaata acggccgac gaaaaaggt 
      atcctgcat ttgttcccg tggtcgggc atcatcgac 
      gaaaatcag gaattgagc agtacgttc cgggttccg 
      gatctcacc accggaaga atgtcgcgt cttattacg 
      caattatcg ccgaaagag gaagaaatg ttccggaac 
      atgattcgg cggatgaat accatcgtt aaggccgcc 
      caagatatg gatgttagg attatggtg gacgcggaa 
      caaacctat ttccagccg gctatcact cgtatcact 
      ctcgaattt atgcgaaaa tataataaa gataaagcg 
      attgttttc aatacgtat cagtgctat ttgaggaac 
      gctttcgat gaagtcacc accgatatg gaacaagcg 
      aaaagacaa aacttttat ttcggcgct aagttggtg 
      cgaggggct tatctcgaa caggaaagg gcccgcgcc 
      gccgccttg ggctacccc gaccccacc aaccccaat 
      ttcgaggcg acgtccgac atgtaccac aaaaccctg 
      accgagtgt ctgcgacga atcaaagcg ttcaaagat 
      ctaggcgaa gaaaagaag atcgccatc atggtggca 
      tcgcacaac gaagatacc gtacgtttc gccattcaa 
      aaaatgaaa gaattcgga atcgaacct gaagataaa 
      gtgatctgt ttcggccaa ttgttggcc atgtgtgat 
      tatatcacg tttccttta ggccaatcc gggtattct 
      gcgtacaag tacatcccg tacgggcct gtgaacgaa 
      gtcttgccc tatttgtcg cggagggcg cacgagaat 
      aagggcgtg ttgaagaaa attaagaag gagaagagg 
      cttctaggg agtgagatt ttgagacgt gtggcgacc 
      gggcaatgg tggtacaca ccgaaggga gactacacc 
      ccggtttaa
    mRNA sequence
    • >TC006024 RA
      ttcggcaag tgcgacatg tcgtcacca gagggcgtc 
      tagttaatt ttatctccg aaaaattta gtattgcca 
      accgaaccg gtcaacaat caagttgac cggtttgca 
      aaaattcga atttggcgc caatccgtt ggtcgccaa 
      gtgtgctgt gcttttcct tccatcagt cacgcaaac 
      gtcacctgt caccaccat cgaaaaaag tcaaaaaat 
      ctgtcatcc gtatcaaat ttcgcgtgt aaatccctt 
      ccggaagcg cgttattcc ccttccggt ggtcctgcg 
      cgtcccagt cgcgcactc cccgccgac gaatcccga 
      cgtcgcatt cagtcgctt gtgaaccag tgaaggtgt 
      ttgagatcc ggtcgtgtg atggctttt ctgcgttcg 
      ctgtcccgg agcagcgcg tccaggtcg tacggcctg 
      ctggtggag ctctcccgc ctccgtctc gccgccgcc 
      aagtcgacg gccgccgtg gtgcaggac gccgccgcc 
      gcccccgtc gccggcaag cggcgcgac cccctcgac 
      atcaccttc gaggacgcc aaggccgcc ttcaagagc 
      aagaccaac tgggagctg atgcgcgcc tacatcgtc 
      tacacgctc tgcagcttc gagaccctc gtcgagaac 
      aacatgcag ctgatgaaa ctatgtaaa ggtatccta 
      ggagaaaga ctgtttata atgctgatg cgagcaacg 
      ttctacggg cacttcgtt gccggcgaa gatcaatac 
      aaaatacga ccgactttg gagcgattg cgatcgttc 
      ggcgtcaag ccgatcctc gactactcg gtcgaagag 
      gacctatcg caagaagag gccgaaaaa cgcgaagtc 
      gaagcttcg gttcccgaa gccggcgat caggtcgaa 
      cagggttcg ttgaaacag taccacgtg gataaaaca 
      ttcgccgat cgacgttat aaggtccaa tcagcacgc 
      acgtacttc tacttgaac gaagcgacc tgcgaacgc 
      aacatggag accttcatc aagtgcttg gaggccgtc 
      gcggacttt cagaagaat ccgtcaggt gccacatac 
      ggcactggc atcaccgcc attaaatta acggccctt 
      ggcagacct caactattg cttcaatta tcggaagta 
      atcatgaaa gctcgtaaa tacatgacg gaaatcgtg 
      ggcggcgaa ggcaacgtc ctctcccat cacatgacc 
      atccaagat ctcgaacgt aaactttcg aaaatgaaa 
      gatcaagaa tcggtgaaa aagttcctg tcgcaaata 
      acggccgac gaaaaaggt atcctgcat ttgttcccg 
      tggtcgggc atcatcgac gaaaatcag gaattgagc 
      agtacgttc cgggttccg gatctcacc accggaaga 
      atgtcgcgt cttattacg caattatcg ccgaaagag 
      gaagaaatg ttccggaac atgattcgg cggatgaat 
      accatcgtt aaggccgcc caagatatg gatgttagg 
      attatggtg gacgcggaa caaacctat ttccagccg 
      gctatcact cgtatcact ctcgaattt atgcgaaaa 
      tataataaa gataaagcg attgttttc aatacgtat 
      cagtgctat ttgaggaac gctttcgat gaagtcacc 
      accgatatg gaacaagcg aaaagacaa aacttttat 
      ttcggcgct aagttggtg cgaggggct tatctcgaa 
      caggaaagg gcccgcgcc gccgccttg ggctacccc 
      gaccccacc aaccccaat ttcgaggcg acgtccgac 
      atgtaccac aaaaccctg accgagtgt ctgcgacga 
      atcaaagcg ttcaaagat ctaggcgaa gaaaagaag 
      atcgccatc atggtggca tcgcacaac gaagatacc 
      gtacgtttc gccattcaa aaaatgaaa gaattcgga 
      atcgaacct gaagataaa gtgatctgt ttcggccaa 
      ttgttggcc atgtgtgat tatatcacg tttccttta 
      ggccaatcc gggtattct gcgtacaag tacatcccg 
      tacgggcct gtgaacgaa gtcttgccc tatttgtcg 
      cggagggcg cacgagaat aagggcgtg ttgaagaaa 
      attaagaag gagaagagg cttctaggg agtgagatt 
      ttgagacgt gtggcgacc gggcaatgg tggtacaca 
      ccgaaggga gactacacc ccggtttaa atgtaagtc 
      agggttgcc aacgtggcc gagattagt tttttgggg 
      ttgtaggag atacgaatt ggagtactt ttttatcga 
      gcctccctg gggtctgag ttagataag tagttgcaa 
      aaaaattca aaagacagt ccaccggat aaaaagtcg 
      cctctgtgt gggagattt cgtccgtag gtataagca 
      aaagtctga ttttattaa ggacaatag tataagagt 
      aacaagctc gatgagggc gaaagtttc acacagatc 
      cggagtttt ttagcgctt agtaattaa taaatttag 
      ttagattta tgaaattat gtacagatg ttcagtgtg 
      ttttaaaaa ataaaatta accaaaaaa aatcgggtg 
      tttgtcggc gtgcttgtg gtaattcgt ttcaaaaat 
      tttatttgg aaggaggcg tcgcgtgga agataaatg 
      acggtgaat cttagtaat ttattagtt tgttgaaaa 
      atttgtatc atttttata gcctgtgat gatagtttt 
      ccttgtttt cagtgtcaa aatctgcct ttgagtatt 
      ttttaaact gtttagttg gtgtgattg aataaattt 
      aattgaagt aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY