Status
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Gene information
General information
TC031270
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006022,TC006021
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2874419..2918371
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031270
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00977

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    tropharium size decreased - Number of animals showing the phenotype: 4
    previtellogenic egg chamber number increased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber complete not present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 7 (11 dpi)
    Days post injection


    larva lethality late (L7) - Number of animals showing the phenotype: 7 (16 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031270 RA
      MSYSQRQPQ NHRPNHSQA TTDIYKPSR SPLSTTARY 
      PYPASPQLP QSQPPTISQ VQVSQRYPP GTAIYTGGY 
      RDYRPQRIS LLHPEYASG RSRESLPGG AGVVHLDSV 
      SQGQQPIKK IRLQEHKDN IQPLRIDTR EQPGTYNPQ 
      QEEQTFRTT KDELIQQIG KVDREIAKA ESQITILKK 
      KEKELEEIA NKPSVKSEV EEDAQPKHQ SLPQKIYAE 
      NRKKAQSAH AKLDSLGPK VEWPLYNQP SDTAVYHEN 
      KRKHMAFKK RLLEYFKRR HAEKDARNA YLTETYSKL 
      MQEWLRKVD KIEGSTKRK AKEAKNREF FEKVFPELR 
      KQREDKERF NRVGARVKS EADMEEIMD NLQEQAMED 
      KKMRSYAVI PPILLDSKE RKITYQNDN GFIEDMEMV 
      YKSRQFLNI WTPSEKEVF KEKYLQHPK NFGVIASFI 
      ERKSVADCV QYYYLSKKT ENYKQLLRK SRQRTRSSR 
      NNTQKVNSS ANTSVVDIL TTGVTTRLQ REQQQQRTV 
      VQQNREPVS EAPPTSTAP TSVPSSTTA ATTTSTTTT 
      ASSTPPSTS PSATSTTTV TVTTSATAT PTAAASSTT 
      STTTTTTVV STATPVMST AASVVTAAA PPTSPKPAD 
      NATADNNAA AAKTTYNEF NSTTTTTTF DDYRSQKFF 
      GDDAKGDGS ATAADAAHD GVVVTVKKE NEPSVQEVA 
      SGDQTTSAT PTAIAETKK KKERRKDKE NANVETSDE 
      ENADIPDKT IQGTCIVCQ AQLGPHLQS RPLVPSQAS 
      QYGLREDQV PGGGRVCNT CRCKCVRSR YTHCPLPSC 
      PNSRGRVKR LRSFPQRLQ DLPPDVREP LLAEFHVPS 
      GVTKCCSAC FNRIQRRLG PVEEWTEDE INQLKNCLT 
      ENGANWQLI GERLNKPAN QIRAFYSAN RKRLNLESC 
      LGDRKPTLT DEEESGSST SSCEEPMRD RHSSDTASA 
      AESPPQAPP TNQNPSKKE DYDSSATET ADEAQTPDH 
      YQGSATITP VTNDGQIRQ NASPLSVKD LVLNVIEFQ 
      LMKNPGGQV QNPPTSGMA PTISSILNN DSSEVTIVS 
      EYNLNTRPP RSDISLAKL VTPLIGATI TPVPATPHQ 
      PPPEPRNDL VVLSVQDRE PETLDLSIK KPREPPPPA 
      HSKHPPMHR EYLPHHAFP IRTQPKLPS PKPANPKSG 
      SITLGTPII NQPRYEGLL RQMAPETKM GSITQGTPI 
      HMPHLQEKR VYEFFNKRG PPPSGGYQY RQPAAYTVE 
      QQLNSRQII MNDYITSQQ MLGRREKPY YARTPPPSN 
      QNQQRQGVI QRHTRPYLP PGHEALSSL VDVAVQQPS 
      LPVPTAPHE GLGKTMADN IMEQPHRFQ IIQQQMRQQ 
      QRMEEQRRI VYQNSRHQS RNTETSLTA ASLIDAIIT 
      HQINQTSEG GREVINNPR EQRPGDLLF QSFHRDPPP 
      NNPPNDNER PPSVISIDL ESDTVNKNL TVKELTDSV 
      ISHDFSTRG PNYHYMRIQ KDDKRSATP QQDERQIIR 
      IAQPQKYHV EPVSPPDNH WGRYQQQQP QNQHISALD 
      YVKNRIVEV MRTEDDKKE DSQEKERSD SPGEMVIDE 
      EGQQFGHCY TANDSSRNN EPKPLLSAQ YDPLSDED
    CDS sequence
    • >TC031270 RA
      atgtcatat tcgcaaaga caaccccag aatcataga 
      ccgaaccac agtcaagcc acgacggac atttacaag 
      ccttcgcgt tcgccattg tcgacaaca gcccgctac 
      ccgtacccc gcatccccc caactcccc caatcgcag 
      cccccgacc atcagtcag gtgcaagtg tctcagcgg 
      taccccccg gggacggcg atctacacg ggcggttat 
      cgcgactac cgcccccaa cgcatctcc ctcctccat 
      cccgaatat gcgagcgga cggtcccgg gagagcctc 
      cctggaggc gccggcgtc gtccatctc gacagcgtc 
      tcccaggga caacagccc atcaagaaa atacgcctg 
      caggagcac aaggataac atacaacct ttgaggatc 
      gatacgagg gagcagccg ggcacctac aacccccaa 
      caggaggag cagacgttt cgcacgacc aaagacgaa 
      ctgatccag cagatcggg aaggtcgat cgtgagatt 
      gcgaaagcc gaaagtcag ataactatc ttgaagaag 
      aaggaaaaa gagttggaa gaaattgca aataagccg 
      tccgttaaa tccgaagtt gaagaagat gcacaaccg 
      aagcatcaa agtcttccg cagaagatc tatgcggag 
      aatcggaag aaggcacaa agtgcgcac gccaagttg 
      gattcgttg ggaccgaaa gtcgaatgg ccgctctac 
      aatcaaccg agtgataca gctgtgtat cacgaaaac 
      aagcggaaa catatggct ttcaaaaag cgacttttg 
      gagtatttc aagcgaaga catgccgaa aaagacgca 
      agaaatgcc tatttgacc gaaacgtac agtaagctg 
      atgcaagaa tggctgagg aaagtcgac aagattgaa 
      ggcagtacg aaacgaaag gccaaggag gcgaaaaat 
      cgcgagttt ttcgagaag gtttttccc gaattgcga 
      aagcaacgg gaggataag gaaagattt aatcgtgtc 
      ggcgctcgc gtgaagagc gaagccgac atggaggag 
      ataatggac aacctccaa gagcaagca atggaggac 
      aagaagatg cgtagttac gccgtgatc ccgcccatt 
      cttctcgat tcgaaagaa cgcaaaatc acgtatcag 
      aacgataac ggctttatc gaggacatg gaaatggtg 
      tataagagt cggcaattt ctcaatata tggacaccc 
      agtgaaaaa gaagttttc aaagagaaa tacctccaa 
      catccgaaa aatttcggt gtgatcgct tcgttcatt 
      gaacgaaaa tcggtcgcg gactgtgtt caatattat 
      tatcttagc aaaaagacc gaaaactat aaacaactt 
      ttaaggaag tcgagacag aggacgcgc tcgtcgagg 
      aacaacacg cagaaggtc aacagtagt gcgaatacc 
      tcggttgtg gatatttta acgacaggg gtgacgacc 
      cgcctccag cgcgagcag caacaacag cgcacggtg 
      gtgcaacag aaccgcgag ccggtttcg gaggcgccc 
      cccacgtcc accgccccc acgtccgtc ccgtcatct 
      acaacagcc gccaccacc acctccacc acaacgacg 
      gccagttcg acgccgccg tcgacaagt ccttcagct 
      acgtctaca acaacagtt actgttacg acatcggcg 
      acggccacc cccaccgcc gccgcctcc tcgacaacg 
      tcgacgacg actacgaca acggtggtc tccaccgcg 
      acgccggtc atgtcgacg gcggcgtca gtggtgacg 
      gctgcagcg ccgccaacg tcgcccaaa ccggccgat 
      aacgccacc gccgataac aacgccgcc gccgccaaa 
      accacctac aatgaattc aattcgacg acgacgacg 
      acaacgttt gacgattat cgaagtcaa aagtttttt 
      ggggacgac gccaagggg gacggtagc gcgacggcc 
      gccgacgcg gcccacgat ggcgtcgtc gtcacggta 
      aagaaagag aatgagccg tcggtacag gaagtcgcc 
      agtggcgac caaacgacc tctgcgact ccaacggcg 
      atcgccgaa accaaaaag aagaaagag agaagaaaa 
      gacaaagag aacgccaat gtggagact agtgacgag 
      gaaaacgcc gatataccg gataaaacc atccaaggc 
      acgtgtatc gtgtgtcaa gcacagctg ggccctcat 
      ctgcagtcg cgccctctg gtgccaagt caggcgtca 
      cagtacggc ttgagggag gatcaagta ccaggcggg 
      ggtcgagtg tgtaacact tgtcggtgt aaatgtgtg 
      aggtcgagg tacacacac tgccccctt ccctcatgc 
      cccaactca cgtgggcgg gtcaaacga ctccggtct 
      ttcccccaa cgattgcaa gatttacca ccggatgtg 
      cgggagccc ctccttgcg gagttccac gtcccgagt 
      ggcgttaca aagtgttgc tccgcctgt tttaaccga 
      attcaacgt cggttagga ccggttgaa gaatggacc 
      gaagacgaa atcaaccaa ttgaagaat tgtctaacc 
      gaaaacggt gctaactgg cagctgatt ggcgaacgt 
      ttaaacaaa ccggccaat caaatccgg gcgttctat 
      tccgccaat cgcaaacgt ctgaatttg gagagttgt 
      ctaggtgat cgtaaacca accttaacc gatgaggaa 
      gaaagtggc tcgagtacg tcaagctgt gaggaacca 
      atgagagac cgacattcc agtgatacg gcaagtgcg 
      gccgaaagc ccgccccaa gccccgccc accaatcag 
      aacccgtcg aaaaaggag gattacgat tcgtcggcg 
      accgaaaca gccgacgaa gcccaaacc cccgatcac 
      tatcaaggc tccgccacc ataacccca gtcaccaat 
      gatggacaa atccgacag aacgcctcc ccattaagt 
      gtcaaagac ttggtcctc aacgtcatc gaattccaa 
      ctaatgaag aatcccgga ggccaagtc cagaacccc 
      ccaactagt ggaatggcc cccaccata tcctcaatc 
      ctcaataac gactcctcc gaagtcaca atcgtgagt 
      gagtacaac ctcaataca cgcccacca cgatccgac 
      atttcattg gctaaactc gtaacgccc ctaatcgga 
      gcgacaata accccagtc ccggccacg ccccaccaa 
      cccccaccc gaaccccga aacgacctc gtcgtcctc 
      tccgtacaa gaccgcgaa ccggaaacg ctagacctc 
      agcattaaa aaaccaaga gaaccgcct cctcccgcc 
      cactcaaaa cacccacca atgcaccgc gagtatctc 
      ccacatcac gccttccca atccgcacc caacccaaa 
      ctcccctcc cctaaacca gccaatccc aaatcgggc 
      tccataact ctgggaacg cccattata aaccaacca 
      cgctacgag ggcttgctc cggcaaatg gcccccgag 
      accaaaatg ggctcgatc acgcaaggc acgcccatc 
      cacatgccc cacctccag gaaaaacgc gtctatgag 
      ttttttaat aaaaggggt cccccacct tcagggggc 
      tatcaatac cgacaaccg gcggcctac accgtcgaa 
      caacaatta aactcgcgc caaatcata atgaacgac 
      tatataaca tcgcaacag atgttgggg cggcgcgag 
      aaaccttac tacgcgcgg actccgccc cctagcaac 
      cagaaccaa caaagacaa ggggtgatt caaagacac 
      acccgcccg tatctgccc ccaggccat gaggcactt 
      tcgagtctc gtggacgtg gccgtacaa cagcccagt 
      ctgcccgtc ccgacagcc ccccacgag gggctcggc 
      aaaacaatg gccgacaac ataatggaa caaccccat 
      cgcttccaa atcatccaa caacagatg cgccaacaa 
      cagcgaatg gaggaacaa agacgtatt gtttatcaa 
      aactcgcgc caccaatcg cgcaacact gaaacctcc 
      ctgacagcg gccagcttg atcgacgcc atcatcacg 
      catcagatc aaccaaacg agtgaaggc ggccgtgaa 
      gtgataaac aacccgcgt gaacaacgt ccgggagac 
      ctacttttc cagagtttc catcgggac cccccgccc 
      aacaacccg ccgaatgac aacgagcgg ccgccgagt 
      gtcatcagt attgatctc gaatcggat actgtcaat 
      aagaatttg acggtgaaa gaattgaca gattcggtg 
      ataagtcac gattttagc acgcgcggt ccgaattat 
      cactatatg cggattcag aaggatgat aagcgctcg 
      gctacgcct cagcaggac gaacgccag attatacgt 
      attgcgcag ccgcagaag tatcacgtg gagccggtg 
      tcgccgcct gacaaccac tgggggcgc taccagcag 
      cagcagccg cagaatcag cacatctcg gcgctggat 
      tacgtgaag aaccgaatt gtggaggtt atgcggacg 
      gaggacgac aagaaggag gacagtcag gagaaggag 
      aggagcgac agtcctggc gaaatggtg atagacgag 
      gaggggcaa caattcggg cattgttac actgccaat 
      gacagttcg aggaataac gagccgaag ccgttgctt 
      tctgcgcag tacgatccg ttatcggac gaggattag
    mRNA sequence
    • >TC031270 RA
      ggacgctct ggggggctc tagtgccgc tcgagtctt 
      agcgagtgc ggttttgtg ccaaattgt cggtttttg 
      gccgtagtg agacggagg gcggtgtgt cggtgatag 
      tagcggcca gcgtctagc ttcatctcg cttgtagta 
      tgcgtcctc ccctttgga aaagatcga ctcatccgg 
      acctgtttt cgtttcagc ttcgaagtt taaccaaga 
      tgtcgggtt atggcggta tctatatta tagaatagt 
      gttccgagt gtttaaagc gtaaattcg gcccgaaat 
      aacccaccc tgtgttgtg tttcgcgtt atttccccc 
      ccagtcaag tgcgtgtgt gtcaaccac cctgcgtca 
      acaatcgac gatcggcca cgtttcgcc cccgaaaag 
      cgcgttttt gtccccaat ttggtgctt ttgcccgat 
      tcatgtgcg ctgagacac ccggggggc ccttcgtcg 
      ctacatacg tggccaagc gcgcgccag ctactttct 
      ccgttgcga tcgcggtgg cctggcata ccatgtaaa 
      atcgggggt ggttttccc aatttccag tgtctgagt 
      gcgtgcaga atatggggg gcagagtga aatcagtgg 
      gggccggac aatatagtg gcggttata cagtgtgtt 
      catgaattg agaaaatca ccagttttt cggcgtaaa 
      tgtatatga tatggaaat acattccgt gagaattga 
      acatgggcc tgtacaaac tccgacaat gtcatattc 
      gcaaagaca accccagaa tcatagacc gaaccacag 
      tcaagccac gacggacat ttacaagcc ttcgcgttc 
      gccattgtc gacaacagc ccgctaccc gtaccccgc 
      atcccccca actccccca atcgcagcc cccgaccat 
      cagtcaggt gcaagtgtc tcagcggta ccccccggg 
      gacggcgat ctacacggg cggttatcg cgactaccg 
      cccccaacg catctccct cctccatcc cgaatatgc 
      gagcggacg gtcccggga gagcctccc tggaggcgc 
      cggcgtcgt ccatctcga cagcgtctc ccagggaca 
      acagcccat caagaaaat acgcctgca ggagcacaa 
      ggataacat acaaccttt gaggatcga tacgaggga 
      gcagccggg cacctacaa cccccaaca ggaggagca 
      gacgtttcg cacgaccaa agacgaact gatccagca 
      gatcgggaa ggtcgatcg tgagattgc gaaagccga 
      aagtcagat aactatctt gaagaagaa ggaaaaaga 
      gttggaaga aattgcaaa taagccgtc cgttaaatc 
      cgaagttga agaagatgc acaaccgaa gcatcaaag 
      tcttccgca gaagatcta tgcggagaa tcggaagaa 
      ggcacaaag tgcgcacgc caagttgga ttcgttggg 
      accgaaagt cgaatggcc gctctacaa tcaaccgag 
      tgatacagc tgtgtatca cgaaaacaa gcggaaaca 
      tatggcttt caaaaagcg acttttgga gtatttcaa 
      gcgaagaca tgccgaaaa agacgcaag aaatgccta 
      tttgaccga aacgtacag taagctgat gcaagaatg 
      gctgaggaa agtcgacaa gattgaagg cagtacgaa 
      acgaaaggc caaggaggc gaaaaatcg cgagttttt 
      cgagaaggt ttttcccga attgcgaaa gcaacggga 
      ggataagga aagatttaa tcgtgtcgg cgctcgcgt 
      gaagagcga agccgacat ggaggagat aatggacaa 
      cctccaaga gcaagcaat ggaggacaa gaagatgcg 
      tagttacgc cgtgatccc gcccattct tctcgattc 
      gaaagaacg caaaatcac gtatcagaa cgataacgg 
      ctttatcga ggacatgga aatggtgta taagagtcg 
      gcaatttct caatatatg gacacccag tgaaaaaga 
      agttttcaa agagaaata cctccaaca tccgaaaaa 
      tttcggtgt gatcgcttc gttcattga acgaaaatc 
      ggtcgcgga ctgtgttca atattatta tcttagcaa 
      aaagaccga aaactataa acaactttt aaggaagtc 
      gagacagag gacgcgctc gtcgaggaa caacacgca 
      gaaggtcaa cagtagtgc gaatacctc ggttgtgga 
      tattttaac gacaggggt gacgacccg cctccagcg 
      cgagcagca acaacagcg cacggtggt gcaacagaa 
      ccgcgagcc ggtttcgga ggcgccccc cacgtccac 
      cgcccccac gtccgtccc gtcatctac aacagccgc 
      caccaccac ctccaccac aacgacggc cagttcgac 
      gccgccgtc gacaagtcc ttcagctac gtctacaac 
      aacagttac tgttacgac atcggcgac ggccacccc 
      caccgccgc cgcctcctc gacaacgtc gacgacgac 
      tacgacaac ggtggtctc caccgcgac gccggtcat 
      gtcgacggc ggcgtcagt ggtgacggc tgcagcgcc 
      gccaacgtc gcccaaacc ggccgataa cgccaccgc 
      cgataacaa cgccgccgc cgccaaaac cacctacaa 
      tgaattcaa ttcgacgac gacgacgac aacgtttga 
      cgattatcg aagtcaaaa gttttttgg ggacgacgc 
      caaggggga cggtagcgc gacggccgc cgacgcggc 
      ccacgatgg cgtcgtcgt cacggtaaa gaaagagaa 
      tgagccgtc ggtacagga agtcgccag tggcgacca 
      aacgacctc tgcgactcc aacggcgat cgccgaaac 
      caaaaagaa gaaagagag aagaaaaga caaagagaa 
      cgccaatgt ggagactag tgacgagga aaacgccga 
      tataccgga taaaaccat ccaaggcac gtgtatcgt 
      gtgtcaagc acagctggg ccctcatct gcagtcgcg 
      ccctctggt gccaagtca ggcgtcaca gtacggctt 
      gagggagga tcaagtacc aggcggggg tcgagtgtg 
      taacacttg tcggtgtaa atgtgtgag gtcgaggta 
      cacacactg cccccttcc ctcatgccc caactcacg 
      tgggcgggt caaacgact ccggtcttt cccccaacg 
      attgcaaga tttaccacc ggatgtgcg ggagcccct 
      ccttgcgga gttccacgt cccgagtgg cgttacaaa 
      gtgttgctc cgcctgttt taaccgaat tcaacgtcg 
      gttaggacc ggttgaaga atggaccga agacgaaat 
      caaccaatt gaagaattg tctaaccga aaacggtgc 
      taactggca gctgattgg cgaacgttt aaacaaacc 
      ggccaatca aatccgggc gttctattc cgccaatcg 
      caaacgtct gaatttgga gagttgtct aggtgatcg 
      taaaccaac cttaaccga tgaggaaga aagtggctc 
      gagtacgtc aagctgtga ggaaccaat gagagaccg 
      acattccag tgatacggc aagtgcggc cgaaagccc 
      gccccaagc cccgcccac caatcagaa cccgtcgaa 
      aaaggagga ttacgattc gtcggcgac cgaaacagc 
      cgacgaagc ccaaacccc cgatcacta tcaaggctc 
      cgccaccat aaccccagt caccaatga tggacaaat 
      ccgacagaa cgcctcccc attaagtgt caaagactt 
      ggtcctcaa cgtcatcga attccaact aatgaagaa 
      tcccggagg ccaagtcca gaacccccc aactagtgg 
      aatggcccc caccatatc ctcaatcct caataacga 
      ctcctccga agtcacaat cgtgagtga gtacaacct 
      caatacacg cccaccacg atccgacat ttcattggc 
      taaactcgt aacgcccct aatcggagc gacaataac 
      cccagtccc ggccacgcc ccaccaacc cccacccga 
      accccgaaa cgacctcgt cgtcctctc cgtacaaga 
      ccgcgaacc ggaaacgct agacctcag cattaaaaa 
      accaagaga accgcctcc tcccgccca ctcaaaaca 
      cccaccaat gcaccgcga gtatctccc acatcacgc 
      cttcccaat ccgcaccca acccaaact cccctcccc 
      taaaccagc caatcccaa atcgggctc cataactct 
      gggaacgcc cattataaa ccaaccacg ctacgaggg 
      cttgctccg gcaaatggc ccccgagac caaaatggg 
      ctcgatcac gcaaggcac gcccatcca catgcccca 
      cctccagga aaaacgcgt ctatgagtt ttttaataa 
      aaggggtcc cccaccttc agggggcta tcaataccg 
      acaaccggc ggcctacac cgtcgaaca acaattaaa 
      ctcgcgcca aatcataat gaacgacta tataacatc 
      gcaacagat gttggggcg gcgcgagaa accttacta 
      cgcgcggac tccgccccc tagcaacca gaaccaaca 
      aagacaagg ggtgattca aagacacac ccgcccgta 
      tctgccccc aggccatga ggcactttc gagtctcgt 
      ggacgtggc cgtacaaca gcccagtct gcccgtccc 
      gacagcccc ccacgaggg gctcggcaa aacaatggc 
      cgacaacat aatggaaca accccatcg cttccaaat 
      catccaaca acagatgcg ccaacaaca gcgaatgga 
      ggaacaaag acgtattgt ttatcaaaa ctcgcgcca 
      ccaatcgcg caacactga aacctccct gacagcggc 
      cagcttgat cgacgccat catcacgca tcagatcaa 
      ccaaacgag tgaaggcgg ccgtgaagt gataaacaa 
      cccgcgtga acaacgtcc gggagacct acttttcca 
      gagtttcca tcgggaccc cccgcccaa caacccgcc 
      gaatgacaa cgagcggcc gccgagtgt catcagtat 
      tgatctcga atcggatac tgtcaataa gaatttgac 
      ggtgaaaga attgacaga ttcggtgat aagtcacga 
      ttttagcac gcgcggtcc gaattatca ctatatgcg 
      gattcagaa ggatgataa gcgctcggc tacgcctca 
      gcaggacga acgccagat tatacgtat tgcgcagcc 
      gcagaagta tcacgtgga gccggtgtc gccgcctga 
      caaccactg ggggcgcta ccagcagca gcagccgca 
      gaatcagca catctcggc gctggatta cgtgaagaa 
      ccgaattgt ggaggttat gcggacgga ggacgacaa 
      gaaggagga cagtcagga gaaggagag gagcgacag 
      tcctggcga aatggtgat agacgagga ggggcaaca 
      attcgggca ttgttacac tgccaatga cagttcgag 
      gaataacga gccgaagcc gttgctttc tgcgcagta 
      cgatccgtt atcggacga ggattagaa gttaggtta 
      atgtccaat aggattcga ttaagttta attgtagat 
      taatgtaaa taacttgta taaggtttt atgtaaggt 
      tagggatta gttacataa cctcaaaat ttgatattt 
      atgtcaaaa tggcggcaa gtccagttt gcatttcct 
      aaacgtgtg ttgctatcg cgtgcaatc agtgtcgaa 
      ctttctttt tctggaaat gtaaataaa cccattcat 
      tatatatat aaatagagt gtaagggta ataacttgt 
      aaatacttg tgaattctt ttcctcttc tttttttaa 
      ttttgaatt ctgttttta cgaagtaat ttaagtctt 
      ttgacgctt tcacgtctg cgcaagacg aactgatga 
      tttttctac acactacaa ggacaaatt atacaaaaa 
      tgcttccaa aaagatgta tgtacactg tcgattttt 
      tagtttgta ttttcagat agaaatttg ttattttgt 
      tgaaataaa ttaaatatc agttt
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