Status
Please wait ...
Gene information
General information
TC006014 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 3113488..3114680
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006014
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
        ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
        {{goTerm.showAll ? '[-]' : '[+]'}}
        Status: {{ goTerm.status }}
        Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
        Reference: {{ goTerm.referenceText }}
        Quotation: {{ goTerm.quotation }}
        Submitter: {{ goTerm.name }}

      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_00976

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

      head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      abdomen segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: 40.0% (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: 60.0% (includes death as larva, prepupa, pupa, adult)

      larva lethality - Number of animals showing the phenotype: 4 (11 dpi)
      Days post injection


      eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (16 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (22 dpi)
      Days post injection


      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006014 RA
        MRRVAATRS KVQNSFSYP VPLLSGVPQ GSVLSPILY 
        IFYSSDFPV SDLHQTKTR FFADDTALW TSRTTAASA 
        SRTLQPLLN QISTWTRKW RVKINPTKS TAILFKHPN 
        LTRNKKFDP RDITITLDN TPIDLVPST RYLGITYTH 
        TCSLDTDLQ NTLKKVRNR SNLLRHLMG NFQGCHSRT 
        LLHTYNTFI RPVIEYRAP IYASLPHTK THRIATCER 
        QILRRIHRL DRFHASNSL HQDTNTTPI TDRLHKLQQ 
        NYIKRTLNN NNTIAQNTL NTSHKFLTR DNTLRNRVP 
        RIPRRKLPH PPTALLSAL YPDLPDNLQ LLVEETPLT 
        MRRH
      CDS sequence
      • >TC006014 RA
        atgcggcgt gtggctgcc acccgatca aaagtccaa 
        aattccttt tcttatccc gtccccctt ctctccggg 
        gtccctcaa ggctccgtt ctgtcgccc attctttac 
        attttttac agttctgat tttcctgtt tcagatctt 
        caccagacc aaaacccgc ttcttcgcc gacgacacc 
        gccctctgg acatcccgg accaccgcc gcctcagcc 
        tccaggacc ctgcagccc ctcctcaac cagatctcc 
        acatggact cgcaagtgg agagtgaag atcaacccc 
        acaaaatcc accgccatc ctcttcaaa caccccaac 
        ctcacacga aacaagaaa ttcgaccca cgcgacatc 
        accatcaca ctcgacaac acacccatc gaccttgtg 
        ccatccaca aggtaccta ggaatcacc tacacacac 
        acatgctca ctcgacacc gacctccag aacacgctc 
        aagaaagtc cgcaaccga tccaacctc ctccgccat 
        ctcatggga aacttccaa gggtgccat tcccgcact 
        cttctccac acctacaac acattcatc cgacccgtc 
        atcgagtac cgagccccc atctacgca tctctccca 
        cacaccaag acacatcgc attgccaca tgcgaacga 
        caaatcctt cgccgaatt caccgcctc gaccgcttc 
        cacgccagc aattccctc caccaggac acaaacacc 
        acccccatc acagaccgt cttcacaaa ctccaacaa 
        aactacatc aaacgcaca ctcaacaac aacaacaca 
        attgcccaa aacacactc aacacttca cataagttt 
        ctcacacgc gacaacact cttcgcaac cgagtcccc 
        cggatcccc agaagaaaa ctgccccat ccacccacg 
        gcgcttctc tcagcgctg taccccgac ctgcccgac 
        aatcttcaa cttctcgtt gaagagacg cccctcaca 
        atgagacgc cactga
      mRNA sequence
      • >TC006014 RA
        tgcactgta aaatgcggc gtgtggctg ccacccgat 
        caaaagtcc aaaattcct tttcttatc ccgtccccc 
        ttctctccg gggtccctc aaggctccg ttctgtcgc 
        ccattcttt acatttttt acagttctg attttcctg 
        tttcagatc ttcaccaga ccaaaaccc gcttcttcg 
        ccgacgaca ccgccctct ggacatccc ggaccaccg 
        ccgcctcag cctccagga ccctgcagc ccctcctca 
        accagatct ccacatgga ctcgcaagt ggagagtga 
        agatcaacc ccacaaaat ccaccgcca tcctcttca 
        aacacccca acctcacac gaaacaaga aattcgacc 
        cacgcgaca tcaccatca cactcgaca acacaccca 
        tcgaccttg tgccatcca caaggtacc taggaatca 
        cctacacac acacatgct cactcgaca ccgacctcc 
        agaacacgc tcaagaaag tccgcaacc gatccaacc 
        tcctccgcc atctcatgg gaaacttcc aagggtgcc 
        attcccgca ctcttctcc acacctaca acacattca 
        tccgacccg tcatcgagt accgagccc ccatctacg 
        catctctcc cacacacca agacacatc gcattgcca 
        catgcgaac gacaaatcc ttcgccgaa ttcaccgcc 
        tcgaccgct tccacgcca gcaattccc tccaccagg 
        acacaaaca ccaccccca tcacagacc gtcttcaca 
        aactccaac aaaactaca tcaaacgca cactcaaca 
        acaacaaca caattgccc aaaacacac tcaacactt 
        cacataagt ttctcacac gcgacaaca ctcttcgca 
        accgagtcc cccggatcc ccagaagaa aactgcccc 
        atccaccca cggcgcttc tctcagcgc tgtaccccg 
        acctgcccg acaatcttc aacttctcg ttgaagaga 
        cgcccctca caatgagac gccactgac gacaccgac 
        aagacaccc acgacgaca tccacatcc acagcttcc 
        tagtgcacc atgatttta acttgaact ttcaagttg 
        acaggtata ttttgtgtt cgttgtgcg tattacagt 
        tcgtttgtg tcgtgtttt tttttcttn aaaaatagc 
        gataccaat gctctagat ttaactaaa cgaagatcc 
        ggtgg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY