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Gene information
General information
TC006009 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 3106577..3119751
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006009
    iBeetle screen iB_00975
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006009 RA
      MGQPMTVER VTTRAQLGQ NLTVATQQS SPYLERAQK 
      WFFSIKFIS GALQNCDIL FPRVLLYRS TLTRVKMAK 
      KKVDDKNSL NGNNTKNES DLSSNDDEP NFSDPEGYV 
      DNITDEELL ADILKQKPK ETDGVESVI VVDGVPQVG 
      PDRIEKLKS VINKIFVKF GSIVNEYYP VTENGHTKG 
      YIFLEYSSP VNAAEAVKL TNNFKLDKQ HTFQVNLFT 
      DFTKYEAIP EEWDPPKPQ PYEGQGDVH HYLLEPDAY 
      DQFAVVLGQ GQSVQIWQN TQPDPSLLE ERHAWTHAY 
      IKWSPLGTY LATFHKQGI ALWVGQKFS QYKRYAHAN 
      AQFIDFSPC EKYLVTYSP QGDPQHPQQ KGIIIWDIR 
      TGQEKRSFN PEGPSSWPI FRWSHDDKY FGRVGNDVL 
      SIYETPSFG LLEKKSIKI SGIRDFSWS PTDNIVAYW 
      VAEDKDVPA SVTLLEIPN RNEIRKKNL FNVADCKIH 
      WQKSGDYLC VKVDRYSKV RKEKNETKY SGMYCNFEI 
      FHMREKQIP VDSVESKEP IQAFAWEPV GSKFAVIHG 
      ESPNINVSF YEVRVGQAP ILLKKFEKR ACNHLFWSP 
      GGQFIVLAG LGSNGGGSL EFVDTHEFL VMNTTDHFQ 
      MSDVEWDPT GRYVTTSVS YWKTKVDTG YWIWSFQGK 
      ILKRVNLEK FAQLLWRPR PATLLSEEK QKEIKKNLK 
      KYYPQFESK DRMRQSKAS KELIEKRAA LMKEFNEYR 
      SKRIKEYNE NKQRRLQLR NNIDTDELD ADPQNVEEE 
      IVEFFIKEE TTILD
    CDS sequence
    • >TC006009 RA
      atggggcaa cccatgacc gtcgaaagg gtcaccacg 
      cgagctcaa ttgggccaa aatttaaca gtagcgaca 
      cagcagtca agtccatac ttggaaagg gcccaaaag 
      tggtttttc tccattaaa ttcatttct ggcgcctta 
      caaaactgt gacatttta ttcccgcgc gtccttctt 
      taccggtcg accctcacg cgtgtcaaa atggcgaaa 
      aagaaagtg gacgacaaa aacagcctt aacgggaat 
      aatacgaag aacgagagc gatttatcg tccaacgat 
      gatgaacca aacttcagc gatcccgag ggatacgtc 
      gacaacatc accgatgaa gagctcctg gccgatatt 
      ttgaagcag aagccgaag gagactgac ggtgttgag 
      agcgtgatt gtggtcgac ggtgtcccg caagtgggc 
      cccgaccgt atcgagaaa ttgaaatcc gtcattaat 
      aagattttc gtcaagttc ggatccata gtcaatgag 
      tactatcca gtgaccgaa aacggtcac accaaaggc 
      tacattttc ctggaatac tcctctcca gtcaacgca 
      gctgaagcc gtgaagctc accaataat ttcaagctt 
      gataagcag cacactttc caagtcaat ctcttcact 
      gactttacc aaatatgag gcgattccg gaggagtgg 
      gaccccccg aagccgcag ccctacgag ggccagggg 
      gacgtccac cactatttg ctcgagcct gatgcttac 
      gaccaattt gccgtggtt ttgggacag ggacagtcg 
      gttcaaatt tggcaaaat acgcagcct gatccttcc 
      ttacttgag gagagacat gcttggaca catgcttat 
      atcaagtgg tctccgtta ggtacgtat ttagccact 
      ttccataag caaggtatc gcgttatgg gtgggtcag 
      aagtttagc caatacaaa aggtatgcc catgccaat 
      gcacaattt attgatttt tcgccgtgc gagaagtat 
      ttggtgacg tactcgcct cagggagat ccgcagcat 
      ccgcaacag aaagggatc attatttgg gatataaga 
      actggccaa gaaaagcgt tctttcaac cctgaaggc 
      ccctcctca tggccgatt ttccgctgg tctcacgac 
      gacaaatac ttcggacgc gtgggcaac gacgtcctc 
      tccatctac gaaaccccc tcgttcggt ctccttgag 
      aaaaaatca atcaaaatc agcggcatc cgcgacttc 
      agctggtcg cccaccgac aacatcgtc gcctattgg 
      gtggcagaa gacaaagac gttccagcc agcgtaacc 
      ctccttgaa atccccaac cgcaacgaa atccgcaag 
      aagaacctc ttcaacgtc gccgattgc aaaatccac 
      tggcaaaaa tctggcgat tatctatgc gtcaaagtc 
      gaccgttac tcgaaagtt cgcaaagag aaaaatgag 
      acgaagtac agcggaatg tactgcaac ttcgagatc 
      ttccacatg agggaaaaa cagatcccg gtggatagc 
      gtcgagagt aaggaaccc atccaggcg ttcgcttgg 
      gaaccggtc ggatcgaag tttgcggtc atacatgga 
      gaatcgccg aatattaat gtcagtttt tacgaagtg 
      agagtggga caagcccca attctgctt aaaaagttc 
      gagaaaagg gcgtgtaat cacctgttt tggtcgccc 
      ggaggccag tttattgtt ttggcgggg ttgggatcg 
      aatggtggt ggatcattg gagtttgtt gatacgcac 
      gagtttttg gttatgaat acgacggat catttccaa 
      atgtcggat gtcgagtgg gatccgacg ggaagatac 
      gtcacgacg tccgtttcg tactggaaa accaaagtt 
      gatacaggt tattggatt tggtcgttc caaggaaaa 
      atcctgaaa cgtgttaat ttggaaaaa ttcgcccag 
      ttgttgtgg cgtccacga cctgcaact ttattgtct 
      gaagaaaag cagaaagaa atcaaaaag aacctcaaa 
      aagtattat ccacagttt gagagcaaa gacagaatg 
      agacaatcg aaagcttca aaggaactt atcgaaaaa 
      cgtgccgct ttaatgaag gagtttaac gaatacaga 
      agtaaaagg attaaggaa tataacgag aataagcaa 
      agacgactt caacttaga aataatatt gataccgat 
      gaattagat gccgaccca caaaacgta gaagaagaa 
      attgtagaa ttctttatt aaagaggaa acaaccatt 
      ttggactaa
    mRNA sequence
    • >TC006009 RA
      cgattgcaa ttgtgcaaa attgataaa aatgcaatc 
      agttcacta cttacattt tgggggccg ttgataagg 
      cggaaaata cgttttaag caccaccgc ccggattaa 
      accacggag cgcgaatcg cttcacttt atggtcaat 
      acagaacag gttgaagaa atataaagg agcttcaag 
      tttgactct atcccacct atttcttaa ccacaacac 
      acctacatg gacagagtt acaatcgac atttcatgc 
      tagcctgag cagcgcatt aatttacct gtgatgggg 
      caacccatg accgtcgaa agggtcacc acgcgagct 
      caattgggc caaaattta acagtagcg acacagcag 
      tcaagtcca tacttggaa agggcccaa aagtggttt 
      ttctccatt aaattcatt tctggcgcc ttacaaaac 
      tgtgacatt ttattcccg cgcgtcctt ctttaccgg 
      tcgaccctc acgcgtgtc aaaatggcg aaaaagaaa 
      gtggacgac aaaaacagc cttaacggg aataatacg 
      aagaacgag agcgattta tcgtccaac gatgatgaa 
      ccaaacttc agcgatccc gagggatac gtcgacaac 
      atcaccgat gaagagctc ctggccgat attttgaag 
      cagaagccg aaggagact gacggtgtt gagagcgtg 
      attgtggtc gacggtgtc ccgcaagtg ggccccgac 
      cgtatcgag aaattgaaa tccgtcatt aataagatt 
      ttcgtcaag ttcggatcc atagtcaat gagtactat 
      ccagtgacc gaaaacggt cacaccaaa ggctacatt 
      ttcctggaa tactcctct ccagtcaac gcagctgaa 
      gccgtgaag ctcaccaat aatttcaag cttgataag 
      cagcacact ttccaagtc aatctcttc actgacttt 
      accaaatat gaggcgatt ccggaggag tgggacccc 
      ccgaagccg cagccctac gagggccag ggggacgtc 
      caccactat ttgctcgag cctgatgct tacgaccaa 
      tttgccgtg gttttggga cagggacag tcggttcaa 
      atttggcaa aatacgcag cctgatcct tccttactt 
      gaggagaga catgcttgg acacatgct tatatcaag 
      tggtctccg ttaggtacg tatttagcc actttccat 
      aagcaaggt atcgcgtta tgggtgggt cagaagttt 
      agccaatac aaaaggtat gcccatgcc aatgcacaa 
      tttattgat ttttcgccg tgcgagaag tatttggtg 
      acgtactcg cctcaggga gatccgcag catccgcaa 
      cagaaaggg atcattatt tgggatata agaactggc 
      caagaaaag cgttctttc aaccctgaa ggcccctcc 
      tcatggccg attttccgc tggtctcac gacgacaaa 
      tacttcgga cgcgtgggc aacgacgtc ctctccatc 
      tacgaaacc ccctcgttc ggtctcctt gagaaaaaa 
      tcaatcaaa atcagcggc atccgcgac ttcagctgg 
      tcgcccacc gacaacatc gtcgcctat tgggtggca 
      gaagacaaa gacgttcca gccagcgta accctcctt 
      gaaatcccc aaccgcaac gaaatccgc aagaagaac 
      ctcttcaac gtcgccgat tgcaaaatc cactggcaa 
      aaatctggc gattatcta tgcgtcaaa gtcgaccgt 
      tactcgaaa gttcgcaaa gagaaaaat gagacgaag 
      tacagcgga atgtactgc aacttcgag atcttccac 
      atgagggaa aaacagatc ccggtggat agcgtcgag 
      agtaaggaa cccatccag gcgttcgct tgggaaccg 
      gtcggatcg aagtttgcg gtcatacat ggagaatcg 
      ccgaatatt aatgtcagt ttttacgaa gtgagagtg 
      ggacaagcc ccaattctg cttaaaaag ttcgagaaa 
      agggcgtgt aatcacctg ttttggtcg cccggaggc 
      cagtttatt gttttggcg gggttggga tcgaatggt 
      ggtggatca ttggagttt gttgatacg cacgagttt 
      ttggttatg aatacgacg gatcatttc caaatgtcg 
      gatgtcgag tgggatccg acgggaaga tacgtcacg 
      acgtccgtt tcgtactgg aaaaccaaa gttgataca 
      ggttattgg atttggtcg ttccaagga aaaatcctg 
      aaacgtgtt aatttggaa aaattcgcc cagttgttg 
      tggcgtcca cgacctgca actttattg tctgaagaa 
      aagcagaaa gaaatcaaa aagaacctc aaaaagtat 
      tatccacag tttgagagc aaagacaga atgagacaa 
      tcgaaagct tcaaaggaa cttatcgaa aaacgtgcc 
      gctttaatg aaggagttt aacgaatac agaagtaaa 
      aggattaag gaatataac gagaataag caaagacga 
      cttcaactt agaaataat attgatacc gatgaatta 
      gatgccgac ccacaaaac gtagaagaa gaaattgta 
      gaattcttt attaaagag gaaacaacc attttggac 
      taagacaat tgaggttga agtgttggt tcctgtgtg 
      cattcaacg tgtgcacgg gatttatag tgactgtga 
      attcataaa cagccttaa ataagaaaa tttacacca 
      attt