Status
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Gene information
General information
TC005994 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 3976892..3997319
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005994
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00974

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 0.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 10.0% (includes death as larva, prepupa, pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (19 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005994 RA
      MRDSLTNPV HVYEGDDAL ITCVVRDIG ENTIMWKKE 
      DRERHSTRV LTAGDKRVT ADKRFAVLH DSSASSDET 
      SDVPSGGDV WVLVIKNAK PSDSGVYVC EVNSNPIVR 
      SFHKLSVLS KALLPPSNT TDTSQYEDQ KQQFSSKAH 
      NYTDCCIAK NVSSNCLGF CNIQSILEG STGQDPENC 
      ESDFPSIVR CMADGRNHV PCCIQERVP DICQDVCRG 
      EYTAITDNI KTHFSCSAY TEQTLACIV EGIELLPSP 
      PQNVEVEAL TEKSLKVSW TPSLSNSET VTNYVINVT 
      SLRAFDEHL IETTSTPKP PMSTHTVQV KVPPSENTT 
      TLNNLNPFT MYEVTVTAV NIHGSSLPS YTVRSLTLT 
      PGKMKTTKV GEAPQLPDI KTCCAKKGI AHKTCINKL 
      CDPALADTA EITDLMICA PWATDTFSC LTNGLDHTP 
      CCKARGLPE LCQQLCTGN VSSIDFNYF KCLRYMGEY 
      TNCLLQGYG VLPSAPTQV HITNIETEF AILHWSAPK 
      TLGDTVLHY NVHYRSVAT FDNEYKAIP KVHSPFILE 
      DLESDTDYE VYVEAVNTH GVGMPSSRI TFRTESKIV 
      EEKIEEAAA YNVTACCVE ADLSSVCLP LCSYNANMS 
      DIKLLAGVC GAEFHKLLR CGAGGRNHG GCCSRRGVP 
      PACLSLCSG VIVDSLLVT ATTCVPYIG NIAQCFEEG 
      TGLLPGPIS ELHATVVDE HSVLLEWEP PAEGVNVSD 
      YVVHYQKVD NTSMHETLL KLDNQVNVT GTTTTLTGL 
      EPESMYHIF VVSRNQHGT SLPSSIIVI KLVKSDGQW 
      VKAVTSPPH SLAVAAHSA TWVTISWQP PEFSHPSEQ 
      ITYRIYHKS TAEENFHVI NVTLTSHMI TNLNPNTQY 
      IIYVTAITE KGASMPSET LIAWTDPAY PAFVEPPTV 
      HPINLVIEG SSMTILCIA MGTPMPTIS LYISGRLVR 
      QETTRHMVT VIHNVTRDM DQISCYADN GYGTPMQAS 
      RKITISHPP HIQASGITM AALGDSVTL ECKVDAQPE 
      PKMIFWKNH QERTPVIQG GKYDINTMP MKDEEDKYV 
      MHLTIKQIT DMDVGDYFC HAENAFGSA TQAVSVRIR 
      NVAATHNVT QCCIEQNVT SSCMDACSF YLDMDAVID 
      KPECINDFD KLMKCAADG SDHRSCCTQ WGVPRRCLD 
      WCRGEPILN SKICVLSYT KQIMSCFHE GREKLPGPP 
      QNVRVENID SHSVNIIWD PPIKNPHTV ELYRVFWRL 
      ADSQGKGGL KGDTSETHL KISDLKEGA TYECVIKAG 
      NSKGTSTLT EPIKFTVGE KYITSAASH SDDSSHVGV 
      AVAVVLALV VVAAIVIAA VWFMRSKKM LIKNSNGVA 
      FENPNKMRE VNMDHIQVP QGQSDGPTV SNGAANGIS 
      VSSASGVQG WKNEALHVP AATEVNPTL YEELKLGQD 
      GAGFKRLKP
    CDS sequence
    • >TC005994 RA
      atgcgagac agtctgact aatcccgtt cacgtctac 
      gaaggcgac gatgctctt attacgtgc gtggttagg 
      gatattggg gagaacacc atcatgtgg aagaaggag 
      gacagggag cggcacagc accagggta ttgacggcg 
      ggggataag agggtgacg gctgataag agattcgcc 
      gtcttgcac gattcatcc gcttcgtcg gatgaaacc 
      tccgacgtg ccttcaggt ggagacgtt tgggtcctg 
      gttataaaa aatgctaaa ccatccgat tcgggagta 
      tacgtgtgt gaagtgaat agtaatccc attgttagg 
      agttttcat aaattaagt gtactgtcg aaagcactg 
      ctaccgccg agtaacaca acggatacc tcgcagtac 
      gaagaccag aagcaacag ttcagtagt aaagctcac 
      aactatacc gactgttgc attgcgaaa aatgtttcg 
      tccaactgt ctagggttt tgcaacatt cagagcatt 
      ctggaaggt agtactggc caagatccc gaaaactgc 
      gagtcagat tttccctca attgtgcga tgcatggca 
      gacggacgc aatcacgtc ccttgctgc atccaggag 
      agagtacca gacatatgc caggatgtg tgtcgcggc 
      gaatacact gcaattacc gacaacatc aaaacacac 
      ttttcttgt tctgcttac accgaacaa actttagct 
      tgcatcgtt gaaggcata gaactcctc cccagccct 
      ccacaaaac gtggaagtg gaagccttg actgaaaaa 
      tccctgaaa gtatcctgg accccttct ttatcaaac 
      tccgaaacc gtcacaaac tacgtaatc aacgtcaca 
      tcgctgcgc gcatttgac gaacactta atcgagaca 
      acttccacc cctaaaccc ccgatgagc acccacacc 
      gtccaggtg aaagtcccc ccaagtgaa aacaccaca 
      actttgaac aatttgaac ccattcaca atgtacgaa 
      gtgaccgta accgctgtc aacatccac ggctccagc 
      ctccctagc tacaccgtt cgctcccta acactcacc 
      cctggcaaa atgaagacc accaaagtg ggcgaagcc 
      ccccagctc cccgatatc aaaacttgc tgtgcaaag 
      aaaggaatc gctcataaa acttgcatc aacaaactt 
      tgtgatccg gctttggcc gacactgca gagattacc 
      gatttgatg atttgcgct ccgtgggcc accgacact 
      ttcagctgt ctgaccaat ggacttgac cacactccc 
      tgttgcaaa gcgaggggt ttgcccgaa ctgtgccaa 
      cagttatgc accgggaat gtctccagt atcgatttc 
      aactacttc aagtgtttg aggtacatg ggggagtac 
      acgaattgt ctccttcaa ggctatggc gttttgccc 
      agtgccccc actcaagtg catatcact aacattgag 
      actgagttt gcgattttg cattggtcg gcgcccaaa 
      actctagga gataccgta ctacattac aatgttcat 
      tatagaagt gttgccacc tttgataat gagtataaa 
      gcgattccg aaggttcac agtccgttt attttggaa 
      gatttggag agtgatacc gattatgag gtctatgtg 
      gaggctgtc aacacccat ggggttggg atgccgagc 
      agtcggatc acgttccgg acagaaagc aagattgtc 
      gaggaaaaa attgaagag gctgccgcg tacaacgtc 
      actgcatgt tgtgtggaa gccgatctc agctcagta 
      tgcctcccc ttgtgctct tacaacgcc aacatgagc 
      gatattaaa ctcttggcg ggagtgtgc ggtgccgaa 
      ttccataaa ctcctccgt tgtggagct gggggccgt 
      aaccatggt ggttgctgt agccgacga ggagtacca 
      ccagcgtgt ctttcgctt tgttctggt gttatagtc 
      gattcacta ctagtcact gctacaact tgtgtgcca 
      tatattgga aatatagct caatgtttt gaagaagga 
      actggactt ctccctgga cccatctcc gaattacat 
      gcaacggtg gtggacgaa cactcggtg ttgctggaa 
      tgggaacca ccagctgaa ggagtgaac gtcagcgac 
      tacgtggtg cattaccaa aaagtggac aacacgtcc 
      atgcatgaa acgctctta aaactagat aaccaagta 
      aatgtgact ggtactacg accactctc acgggactt 
      gaacccgag tcaatgtac cacattttc gtggtttca 
      cgcaaccaa catggtact tctctccca tcgtcgatt 
      atcgtgatc aaacttgta aaatcagat ggtcagtgg 
      gtcaaagct gtgacttca cctccgcat tccctggcc 
      gtggctgcg catagcgcc acctgggtg actatctcg 
      tggcaaccc ccggaattc agccacccc tcggagcaa 
      ataacgtat cgaatttac cacaaatca accgctgag 
      gagaatttc cacgttata aatgtcaca cttacctcg 
      cacatgatc acaaatcta aatcccaac acgcagtat 
      attatttac gttacggcc attacggaa aaaggggcg 
      agtatgccg tcggagact ttgattgct tggacggat 
      ccagcatac ccggctttt gtcgaaccg ccgacagta 
      catccgatc aatttggtg attgaggga agcagtatg 
      acaatcttg tgtattgcc atggggaca ccgatgcct 
      accatttcg ctttatatt tcgggaagg ttagtgcgg 
      caggaaact acgaggcat atggtcaca gtgattcac 
      aacgtgacg agggacatg gaccagatt tcgtgctat 
      gcggataat gggtatggc acgccgatg caagcctca 
      aggaaaatc actatcagt cacccaccc cacatccag 
      gccagtggc ataacaatg gcagctctg ggcgactcg 
      gtcactttg gagtgtaaa gtggacgcc caacctgaa 
      cctaagatg attttctgg aagaaccac caagaacga 
      acaccggtg atccaaggc gggaaatac gacataaac 
      actatgccg atgaaggac gaggaggat aaatatgta 
      atgcatttg acgatcaaa cagatcaca gatatggac 
      gttggtgat tatttctgt cacgctgaa aatgcattc 
      ggtagcgca actcaagcc gtttcggtg cgaattcgc 
      aacgttgcc gcaacacat aacgtaacg cagtgttgt 
      atcgaacag aacgtaacg tcgtcatgt atggacgcc 
      tgttccttc tacctagat atggacgca gtgattgat 
      aaacctgaa tgtatcaac gattttgat aagttaatg 
      aaatgtgca gctgatggt tcggaccac cgcagttgt 
      tgcacgcaa tggggagtt ccccgacgt tgtctcgac 
      tggtgccga ggagagccg atcctcaac agcaaaatc 
      tgcgtcctt tcttacacc aaacaaatc atgagttgt 
      ttccacgaa ggtcgcgag aaattacca ggacctcca 
      caaaacgta cgagttgaa aacatcgac tcacattcg 
      gttaacatc atctgggat ccccccatt aagaacccg 
      cacactgtc gagttgtat cgagtgttc tggcgattg 
      gctgacagt caaggcaag ggtggactg aagggtgat 
      acctcagaa acgcatttg aaaatcagt gatttgaaa 
      gaaggggca acgtatgag tgtgtgatt aaagcgggg 
      aatagtaaa ggaacgtcg actttgacc gaaccgatc 
      aagtttaca gttggtgaa aagtatatt acttcagcg 
      gccagtcat agtgatgat agtagtcat gtgggagtt 
      gctgttgct gttgttttg gcccttgtt gtagtggcg 
      gctattgtg atagcagcg gtttggttc atgaggagc 
      aagaagatg ttgattaag aattcgaat ggagtggct 
      ttcgaaaat ccaaacaaa atgagagag gtcaatatg 
      gaccacata caggtgcca caggggcag agtgacggc 
      cccaccgta tccaacggg gctgccaac ggcatttcg 
      gtgtcgtcc gcctctgga gtgcaaggc tggaagaac 
      gaagctctt cacgtacct gcagcgacc gaagtgaat 
      ccgactctt tacgaagaa ctgaaattg gggcaggat 
      ggtgccggt ttcaagcga cttaaaccc tag
    mRNA sequence
    • >TC005994 RA
      catactaaa cagtcttga aattttgga tttggaaaa 
      tattgttca cacgattgc cctcgatgc gagacagtc 
      tgactaatc ccgttcacg tctacgaag gcgacgatg 
      ctcttatta cgtgcgtgg ttagggata ttggggaga 
      acaccatca tgtggaaga aggaggaca gggagcggc 
      acagcacca gggtattga cggcggggg ataagaggg 
      tgacggctg ataagagat tcgccgtct tgcacgatt 
      catccgctt cgtcggatg aaacctccg acgtgcctt 
      caggtggag acgtttggg tcctggtta taaaaaatg 
      ctaaaccat ccgattcgg gagtatacg tgtgtgaag 
      tgaatagta atcccattg ttaggagtt ttcataaat 
      taagtgtac tgtcgaaag cactgctac cgccgagta 
      acacaacgg atacctcgc agtacgaag accagaagc 
      aacagttca gtagtaaag ctcacaact ataccgact 
      gttgcattg cgaaaaatg tttcgtcca actgtctag 
      ggttttgca acattcaga gcattctgg aaggtagta 
      ctggccaag atcccgaaa actgcgagt cagattttc 
      cctcaattg tgcgatgca tggcagacg gacgcaatc 
      acgtccctt gctgcatcc aggagagag taccagaca 
      tatgccagg atgtgtgtc gcggcgaat acactgcaa 
      ttaccgaca acatcaaaa cacactttt cttgttctg 
      cttacaccg aacaaactt tagcttgca tcgttgaag 
      gcatagaac tcctcccca gccctccac aaaacgtgg 
      aagtggaag ccttgactg aaaaatccc tgaaagtat 
      cctggaccc cttctttat caaactccg aaaccgtca 
      caaactacg taatcaacg tcacatcgc tgcgcgcat 
      ttgacgaac acttaatcg agacaactt ccaccccta 
      aacccccga tgagcaccc acaccgtcc aggtgaaag 
      tccccccaa gtgaaaaca ccacaactt tgaacaatt 
      tgaacccat tcacaatgt acgaagtga ccgtaaccg 
      ctgtcaaca tccacggct ccagcctcc ctagctaca 
      ccgttcgct ccctaacac tcacccctg gcaaaatga 
      agaccacca aagtgggcg aagcccccc agctccccg 
      atatcaaaa cttgctgtg caaagaaag gaatcgctc 
      ataaaactt gcatcaaca aactttgtg atccggctt 
      tggccgaca ctgcagaga ttaccgatt tgatgattt 
      gcgctccgt gggccaccg acactttca gctgtctga 
      ccaatggac ttgaccaca ctccctgtt gcaaagcga 
      ggggtttgc ccgaactgt gccaacagt tatgcaccg 
      ggaatgtct ccagtatcg atttcaact acttcaagt 
      gtttgaggt acatggggg agtacacga attgtctcc 
      ttcaaggct atggcgttt tgcccagtg cccccactc 
      aagtgcata tcactaaca ttgagactg agtttgcga 
      ttttgcatt ggtcggcgc ccaaaactc taggagata 
      ccgtactac attacaatg ttcattata gaagtgttg 
      ccacctttg ataatgagt ataaagcga ttccgaagg 
      ttcacagtc cgtttattt tggaagatt tggagagtg 
      ataccgatt atgaggtct atgtggagg ctgtcaaca 
      cccatgggg ttgggatgc cgagcagtc ggatcacgt 
      tccggacag aaagcaaga ttgtcgagg aaaaaattg 
      aagaggctg ccgcgtaca acgtcactg catgttgtg 
      tggaagccg atctcagct cagtatgcc tccccttgt 
      gctcttaca acgccaaca tgagcgata ttaaactct 
      tggcgggag tgtgcggtg ccgaattcc ataaactcc 
      tccgttgtg gagctgggg gccgtaacc atggtggtt 
      gctgtagcc gacgaggag taccaccag cgtgtcttt 
      cgctttgtt ctggtgtta tagtcgatt cactactag 
      tcactgcta caacttgtg tgccatata ttggaaata 
      tagctcaat gttttgaag aaggaactg gacttctcc 
      ctggaccca tctccgaat tacatgcaa cggtggtgg 
      acgaacact cggtgttgc tggaatggg aaccaccag 
      ctgaaggag tgaacgtca gcgactacg tggtgcatt 
      accaaaaag tggacaaca cgtccatgc atgaaacgc 
      tcttaaaac tagataacc aagtaaatg tgactggta 
      ctacgacca ctctcacgg gacttgaac ccgagtcaa 
      tgtaccaca ttttcgtgg tttcacgca accaacatg 
      gtacttctc tcccatcgt cgattatcg tgatcaaac 
      ttgtaaaat cagatggtc agtgggtca aagctgtga 
      cttcacctc cgcattccc tggccgtgg ctgcgcata 
      gcgccacct gggtgacta tctcgtggc aacccccgg 
      aattcagcc acccctcgg agcaaataa cgtatcgaa 
      tttaccaca aatcaaccg ctgaggaga atttccacg 
      ttataaatg tcacactta cctcgcaca tgatcacaa 
      atctaaatc ccaacacgc agtatatta tttacgtta 
      cggccatta cggaaaaag gggcgagta tgccgtcgg 
      agactttga ttgcttgga cggatccag catacccgg 
      cttttgtcg aaccgccga cagtacatc cgatcaatt 
      tggtgattg agggaagca gtatgacaa tcttgtgta 
      ttgccatgg ggacaccga tgcctacca tttcgcttt 
      atatttcgg gaaggttag tgcggcagg aaactacga 
      ggcatatgg tcacagtga ttcacaacg tgacgaggg 
      acatggacc agatttcgt gctatgcgg ataatgggt 
      atggcacgc cgatgcaag cctcaagga aaatcacta 
      tcagtcacc caccccaca tccaggcca gtggcataa 
      caatggcag ctctgggcg actcggtca ctttggagt 
      gtaaagtgg acgcccaac ctgaaccta agatgattt 
      tctggaaga accaccaag aacgaacac cggtgatcc 
      aaggcggga aatacgaca taaacacta tgccgatga 
      aggacgagg aggataaat atgtaatgc atttgacga 
      tcaaacaga tcacagata tggacgttg gtgattatt 
      tctgtcacg ctgaaaatg cattcggta gcgcaactc 
      aagccgttt cggtgcgaa ttcgcaacg ttgccgcaa 
      cacataacg taacgcagt gttgtatcg aacagaacg 
      taacgtcgt catgtatgg acgcctgtt ccttctacc 
      tagatatgg acgcagtga ttgataaac ctgaatgta 
      tcaacgatt ttgataagt taatgaaat gtgcagctg 
      atggttcgg accaccgca gttgttgca cgcaatggg 
      gagttcccc gacgttgtc tcgactggt gccgaggag 
      agccgatcc tcaacagca aaatctgcg tcctttctt 
      acaccaaac aaatcatga gttgtttcc acgaaggtc 
      gcgagaaat taccaggac ctccacaaa acgtacgag 
      ttgaaaaca tcgactcac attcggtta acatcatct 
      gggatcccc ccattaaga acccgcaca ctgtcgagt 
      tgtatcgag tgttctggc gattggctg acagtcaag 
      gcaagggtg gactgaagg gtgatacct cagaaacgc 
      atttgaaaa tcagtgatt tgaaagaag gggcaacgt 
      atgagtgtg tgattaaag cggggaata gtaaaggaa 
      cgtcgactt tgaccgaac cgatcaagt ttacagttg 
      gtgaaaagt atattactt cagcggcca gtcatagtg 
      atgatagta gtcatgtgg gagttgctg ttgctgttg 
      ttttggccc ttgttgtag tggcggcta ttgtgatag 
      cagcggttt ggttcatga ggagcaaga agatgttga 
      ttaagaatt cgaatggag tggctttcg aaaatccaa 
      acaaaatga gagaggtca atatggacc acatacagg 
      tgccacagg ggcagagtg acggcccca ccgtatcca 
      acggggctg ccaacggca tttcggtgt cgtccgcct 
      ctggagtgc aaggctgga agaacgaag ctcttcacg 
      tacctgcag cgaccgaag tgaatccga ctctttacg 
      aagaactga aattggggc aggatggtg ccggtttca 
      agcgactta aaccctagt atgaggcta gagtaggag 
      atgagtcga gattttagg aaaaaaatc ggtcacgtt 
      attgtgata tagtaataa aaaaatata gtattcgta 
      ccaccggaa aggtatttt acctaaata cctcatata 
      gtgtaatat ttttctctc ctaggtgat aaggttgta 
      aaattgtga ttgagatat atttgggac aggggggag 
      actagatta ttgttagtg aaaatgata aaaaaagat 
      gtaaaatag aaaattttt gtaaatgca cttgtagat 
      tgtaaatta gtaaaaaca gtccaaata tttgtatta 
      ttgataggt taagctaat aattgactg aatgtttta 
      tgatgtagt ggaaaacgt tcatcacgt gtgtgttaa 
      tttgagtga tatttgtta acattccag aattttgat 
      ttttgatca aggaacttg gtcttattg caaaatatc 
      aataccagc acataaata ataaacata gtaattgtt 
      aattaaaat aatatagct tctagtctt atgtaatat 
      aactgttat gtaatagaa ataaagcca tgtttcttg 
      attgtaggt gattcttaa tgagcgatg aatgaggat 
      ttaatatat agattaaaa aatacacgt attgtgcct 
      ttctcactt tcttgtaag gaaatctaa tttcaaaac 
      aacatgtag agattttta gatatttct ttgtacata 
      atttttaaa aatttgtaa aataatatg atgtcacag 
      atagcaaag gcaatagaa ataaatacg aaaaactga 
      a
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