Status
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Gene information
General information
TC033978
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005990,TC005989
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4139140..4161686
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033978
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00973

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 77.78% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 10 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033978 RA
      MKVKDVERT ANVAWSPKN QSPILLAAG TAAQQLDAS 
      FSTNATLEI YSLNLGELG PDMVLKAAT PSEHRFHKI 
      IWGAYESHG TIVGGCDGG LIQIYSASK LMNGENGLI 
      CRQNKHTGP VHALDFNPF QQNLFATGA GDSEIFIWD 
      LNNTNTPMS PGSKSQPLE DVLAISWNR QVQHILAST 
      FASKCVIWD LRKNEPIIK LTDTVSRVR WKMIAWHPE 
      VATQLCLAS EEDHSPIIQ LWDLRFATS PLKTLENHQ 
      RGVLSVAWC ADDPDLLVS CGKDNRIIA WNPNSNEPN 
      GEVLSEIAK TNQWNFDVQ WCPRNPAII ACPSFDGHV 
      SVYSLMGGK TQQIQTTNK IADSFPGMD GYVQAPVLQ 
      QSSAPVSVD LSRPPKWLK RPVGARFGF GGKLISFDN 
      TKPDVDQIS HRTVQISQV ITEPELVQK STELEQALE 
      YGNFTEYCS RKAETTNDQ HTKYIWHFL RANFEENPR 
      AELLNLLGY RIEDVNNKL NQFVHKNKV DGVSERLED 
      LDPFDAIVD NKTVTKPFK IKTGDDTEG LITQALLLG 
      NVEAAVELC LEAKRFADA IIIAMTGGS DLLARTQHA 
      YLEQCEGYV SSLISALVS EDWASVINN CDLSSWKEA 
      LAATLTHST DEALPLLCE QLGNRLSHG DAKLKQDSQ 
      LCYICAGSF DNLVNSWSG NAQNSTSDL QDLVELVVF 
      LQKAVERQG RQVQISGAL ADLLGRYAS ILASQGNLS 
      TALSYLGSS QDEKVAALR ERLYISLGQ KPGYGAQRQ 
      AQSRRLSQR QSFSSYPNM GAQFNSGLP NAAVPVSQP 
      WQPQPQMGG FNQQPPART FSPAPPSQP TRPPSVGSN 
      HGGSGTGLP SRKYVLDPS VTSNPYGAP TRNMYAPQP 
      VSNFQNNNY GAPSPITPL VPSNNNYVP NPVPMVPNP 
      PLNPTPLVP NIQPPPPMT SEMGQPATE KYFPSSAPG 
      WNDPPPLNK PLRPQPKPE ASVPSQPIT HPLYGSAPT 
      NNGYGTEPQ MFQPQPTNN FAPPNNFTP PTTFNPTGF 
      KSNAVNFNQ VQNQTPVVP EKPQPIQKP PIPEEHIHM 
      QTVFDELKY QCSCAANNP QTKRKLEDV GRKLESLYD 
      LLREQKLSP NTLGALHQM VQLVQNGDY TGGLNLHTQ 
      LVSGPDFAQ IASFMPGLK VLLQCALQL QVYLR
    CDS sequence
    • >TC033978 RA
      atgaaagtg aaagacgtg gagcgcacc gccaacgta 
      gcgtggtcc cccaagaat cagagcccc attttgtta 
      gcagccggg acagccgcc caacagctc gacgcctcc 
      ttcagcacc aatgccacc ttggagatc tattccctc 
      aacttgggg gagttgggt cccgatatg gtgctcaag 
      gctgcgacc ccgagcgag caccgcttt cataaaatc 
      atctggggg gcttacgag agtcatggg accatcgtg 
      ggtgggtgt gatgggggc ctgatccag atttacagc 
      gcttccaag ttgatgaat ggggagaat gggctgatt 
      tgtcggcag aataagcac acagggcct gtgcatgcg 
      cttgacttt aaccccttt cagcagaat ttgttcgca 
      acgggggcc ggggattcc gagattttt atctgggat 
      ttgaataac accaacacg cccatgagt cctgggtcc 
      aagtcgcag ccgctggag gacgttctg gcgatttcg 
      tggaatagg caagtgcag catatttta gcgtcgact 
      tttgcctcc aagtgtgtc atttgggat ttgaggaag 
      aacgaaccg attattaaa ttaacggac actgtctcg 
      agggttagg tggaaaatg attgcatgg catccggaa 
      gtggccacg cagttgtgt ttagcttct gaggaggat 
      cactctccc attatccaa ttatgggac ctccgtttt 
      gccacatcc ccccttaaa acactcgag aatcaccaa 
      agaggcgta ttatcagtg gcgtggtgt gccgatgat 
      cccgattta ttagtcagt tgtggtaaa gataatcgt 
      attattgcc tggaatcca aattcaaat gaaccaaac 
      ggagaggtt ctctccgaa attgcaaaa acgaatcaa 
      tggaatttt gatgtacag tggtgtcct agaaatcca 
      gccattatt gcatgtccc agttttgat ggacatgtt 
      tctgtttat tctttaatg gggggcaag acccaacag 
      atacaaacc acgaataaa attgctgat agttttccg 
      ggaatggat ggatatgtt caagctcct gttctacag 
      caaagctcg gcaccagtc agtgtggat ttaagtaga 
      ccaccaaag tggcttaag agaccagtt ggagccaga 
      tttgggttt ggtggcaaa ttaatttcg ttcgataac 
      actaaaccc gacgttgat caaatcagc caccgaacg 
      gttcaaatc agtcaagtt ataaccgaa ccagagcta 
      gtccagaaa tcaaccgaa ctggaacaa gctctcgaa 
      tacggcaac ttcactgaa tactgttcc cgcaaagcc 
      gaaaccaca aacgatcaa cacaccaaa tacatttgg 
      cactttctg cgtgccaac ttcgaggaa aaccctcgc 
      gcggaacta ttgaattta ttgggctat cgcatcgaa 
      gacgtcaat aacaaactc aaccaattc gtccacaaa 
      aacaaagtt gacggagta tcggaacgt ttagaggac 
      ttggatcca tttgacgca attgtcgat aataaaaca 
      gtgacaaaa ccgttcaaa atcaaaact ggtgacgac 
      accgaaggc ttaatcact caagcgctg cttttgggg 
      aacgtcgaa gcggcggtt gagttgtgt ttggaggcg 
      aagcgcttc gccgacgct atcattatt gcaatgact 
      gggggctcg gatttattg gctcggacc caacacgcg 
      tacttggag caatgcgag ggctatgtt tcgtcgctg 
      atttcggct ttggtgtcc gaagactgg gcttctgtt 
      atcaataac tgcgacctg agcagttgg aaggaggca 
      cttgcggcc actctgacg cactccacc gacgaggcg 
      ttgcccctt ctgtgcgaa cagctcgga aatcgcctc 
      agccacggc gacgccaaa ctcaaacaa gactcccag 
      ctgtgctac atctgcgct gggagtttc gacaatctg 
      gtcaactcc tggagcggc aatgcgcag aattccacc 
      agcgatttg caggatttg gtcgagttg gtggtgttt 
      ttgcaaaag gcggtggaa aggcaaggg cggcaggtg 
      cagatttcg ggcgctttg gccgatttg ctgggcagg 
      tatgcgtcg attttggcc tcgcagggg aatttgtcg 
      acggcgttg agttacttg gggagttcg caagacgag 
      aaggttgcg gcgttgagg gagcgcttg tatatcagt 
      ttggggcag aagccgggg tatggggcg cagagacag 
      gcacagagt cggaggttg agtcagaga cagtcgttt 
      tcgagttat ccgaacatg ggggcacag tttaattca 
      ggactgccg aatgcagcg gttccggtg tctcagccg 
      tggcaaccg cagccccaa atggggggt tttaatcaa 
      caaccaccg gcgaggacg ttttcgccg gcgccgccg 
      agtcagccg acgaggccc ccaagtgtg ggatctaac 
      catggtggt tctggcacg ggcttgcct tcgcgtaaa 
      tacgttctg gacccctct gtaacctcc aacccctac 
      ggagcgcct acgaggaac atgtacgcc ccccaacct 
      gtctccaac ttccagaac aataactat ggggcccca 
      tcaccaata acacccttg gttccttcc aataataat 
      tacgtaccc aatcctgta ccaatggta cccaatcca 
      ccactcaat cccactcct ttggtacca aatatccaa 
      ccaccgccg cctatgacg agcgagatg ggccaacct 
      gccacagaa aaatacttt ccttctagt gcccccggc 
      tggaacgat ccacctcca ctaaacaaa ccattgaga 
      ccacagccc aaaccagag gcatccgtc ccatctcaa 
      cccataacg cacccattg tatgggtcg gcgcccacc 
      aacaacggt tacggaacc gaaccacaa atgttccaa 
      ccacagcca actaataat tttgcccca cctaacaat 
      ttcacaccg cctactacg ttcaatccg actggtttt 
      aagtcaaat gcggtcaat tttaaccag gtccaaaac 
      caaactcct gtggttcca gagaaacca caaccaatt 
      caaaaacca ccaattccc gaagaacac atacacatg 
      caaaccgtt tttgacgag ttgaaatat cagtgcagt 
      tgtgcggct aataatccg caaaccaag cgaaaattg 
      gaggatgtt ggccgaaag ttagagtcg ttgtatgat 
      ttgttaagg gaacagaaa ctgtcaccc aacacttta 
      ggagcgtta catcaaatg gtacagttg gtgcaaaac 
      ggagattat acaggagga ctgaattta cacactcag 
      ctagtgtca gggcctgat ttcgctcaa attgccagt 
      tttatgccc gggcttaaa gttttgtta cagtgcgct 
      ttacaattg caagtttat ttaagataa
    mRNA sequence
    • >TC033978 RA
      tgggaaagt gaaagaatg tatatcaaa ttttaaatt 
      ttttgaaac atcgataaa acagaatcc tgcgttacc 
      gaccagtct ttccaatta aaaattccc aaaatttga 
      ttaatttgt ctaaaatcg tatcgctaa tttaattat 
      tcgctttac caccttacg ttagtgtct cataaattt 
      tattgcccc ttgataaca actaaacca atgaaaaaa 
      taagttcgg ttaggtcgt gttgacgtg ttcctgtca 
      tctgattgt cagtcgtaa aataagtta agccacgtt 
      ggatgtctt cccgtgtgt tttacgtta aataagtgc 
      ttaaccatg aaagtgaaa gacgtggag cgcaccgcc 
      aacgtagcg tggtccccc aagaatcag agccccatt 
      ttgttagca gccgggaca gccgcccaa cagctcgac 
      gcctccttc agcaccaat gccaccttg gagatctat 
      tccctcaac ttgggggag ttgggtccc gatatggtg 
      ctcaaggct gcgaccccg agcgagcac cgctttcat 
      aaaatcatc tggggggct tacgagagt catgggacc 
      atcgtgggt gggtgtgat gggggcctg atccagatt 
      tacagcgct tccaagttg atgaatggg gagaatggg 
      ctgatttgt cggcagaat aagcacaca gggcctgtg 
      catgcgctt gactttaac ccctttcag cagaatttg 
      ttcgcaacg ggggccggg gattccgag atttttatc 
      tgggatttg aataacacc aacacgccc atgagtcct 
      gggtccaag tcgcagccg ctggaggac gttctggcg 
      atttcgtgg aataggcaa gtgcagcat attttagcg 
      tcgactttt gcctccaag tgtgtcatt tgggatttg 
      aggaagaac gaaccgatt attaaatta acggacact 
      gtctcgagg gttaggtgg aaaatgatt gcatggcat 
      ccggaagtg gccacgcag ttgtgttta gcttctgag 
      gaggatcac tctcccatt atccaatta tgggacctc 
      cgttttgcc acatccccc cttaaaaca ctcgagaat 
      caccaaaga ggcgtatta tcagtggcg tggtgtgcc 
      gatgatccc gatttatta gtcagttgt ggtaaagat 
      aatcgtatt attgcctgg aatccaaat tcaaatgaa 
      ccaaacgga gaggttctc tccgaaatt gcaaaaacg 
      aatcaatgg aattttgat gtacagtgg tgtcctaga 
      aatccagcc attattgca tgtcccagt tttgatgga 
      catgtttct gtttattct ttaatgggg ggcaagacc 
      caacagata caaaccacg aataaaatt gctgatagt 
      tttccggga atggatgga tatgttcaa gctcctgtt 
      ctacagcaa agctcggca ccagtcagt gtggattta 
      agtagacca ccaaagtgg cttaagaga ccagttgga 
      gccagattt gggtttggt ggcaaatta atttcgttc 
      gataacact aaacccgac gttgatcaa atcagccac 
      cgaacggtt caaatcagt caagttata accgaacca 
      gagctagtc cagaaatca accgaactg gaacaagct 
      ctcgaatac ggcaacttc actgaatac tgttcccgc 
      aaagccgaa accacaaac gatcaacac accaaatac 
      atttggcac tttctgcgt gccaacttc gaggaaaac 
      cctcgcgcg gaactattg aatttattg ggctatcgc 
      atcgaagac gtcaataac aaactcaac caattcgtc 
      cacaaaaac aaagttgac ggagtatcg gaacgttta 
      gaggacttg gatccattt gacgcaatt gtcgataat 
      aaaacagtg acaaaaccg ttcaaaatc aaaactggt 
      gacgacacc gaaggctta atcactcaa gcgctgctt 
      ttggggaac gtcgaagcg gcggttgag ttgtgtttg 
      gaggcgaag cgcttcgcc gacgctatc attattgca 
      atgactggg ggctcggat ttattggct cggacccaa 
      cacgcgtac ttggagcaa tgcgagggc tatgtttcg 
      tcgctgatt tcggctttg gtgtccgaa gactgggct 
      tctgttatc aataactgc gacctgagc agttggaag 
      gaggcactt gcggccact ctgacgcac tccaccgac 
      gaggcgttg ccccttctg tgcgaacag ctcggaaat 
      cgcctcagc cacggcgac gccaaactc aaacaagac 
      tcccagctg tgctacatc tgcgctggg agtttcgac 
      aatctggtc aactcctgg agcggcaat gcgcagaat 
      tccaccagc gatttgcag gatttggtc gagttggtg 
      gtgtttttg caaaaggcg gtggaaagg caagggcgg 
      caggtgcag atttcgggc gctttggcc gatttgctg 
      ggcaggtat gcgtcgatt ttggcctcg caggggaat 
      ttgtcgacg gcgttgagt tacttgggg agttcgcaa 
      gacgagaag gttgcggcg ttgagggag cgcttgtat 
      atcagtttg gggcagaag ccggggtat ggggcgcag 
      agacaggca cagagtcgg aggttgagt cagagacag 
      tcgttttcg agttatccg aacatgggg gcacagttt 
      aattcagga ctgccgaat gcagcggtt ccggtgtct 
      cagccgtgg caaccgcag ccccaaatg gggggtttt 
      aatcaacaa ccaccggcg aggacgttt tcgccggcg 
      ccgccgagt cagccgacg aggccccca agtgtggga 
      tctaaccat ggtggttct ggcacgggc ttgccttcg 
      cgtaaatac gttctggac ccctctgta acctccaac 
      ccctacgga gcgcctacg aggaacatg tacgccccc 
      caacctgtc tccaacttc cagaacaat aactatggg 
      gccccatca ccaataaca cccttggtt ccttccaat 
      aataattac gtacccaat cctgtacca atggtaccc 
      aatccacca ctcaatccc actcctttg gtaccaaat 
      atccaacca ccgccgcct atgacgagc gagatgggc 
      caacctgcc acagaaaaa tactttcct tctagtgcc 
      cccggctgg aacgatcca cctccacta aacaaacca 
      ttgagacca cagcccaaa ccagaggca tccgtccca 
      tctcaaccc ataacgcac ccattgtat gggtcggcg 
      cccaccaac aacggttac ggaaccgaa ccacaaatg 
      ttccaacca cagccaact aataatttt gccccacct 
      aacaatttc acaccgcct actacgttc aatccgact 
      ggttttaag tcaaatgcg gtcaatttt aaccaggtc 
      caaaaccaa actcctgtg gttccagag aaaccacaa 
      ccaattcaa aaaccacca attcccgaa gaacacata 
      cacatgcaa accgttttt gacgagttg aaatatcag 
      tgcagttgt gcggctaat aatccgcaa accaagcga 
      aaattggag gatgttggc cgaaagtta gagtcgttg 
      tatgatttg ttaagggaa cagaaactg tcacccaac 
      actttagga gcgttacat caaatggta cagttggtg 
      caaaacgga gattataca ggaggactg aatttacac 
      actcagcta gtgtcaggg cctgatttc gctcaaatt 
      gccagtttt atgcccggg cttaaagtt ttgttacag 
      tgcgcttta caattgcaa gtttattta agataaggt 
      ctaaccatg tattatttt gttttagat gtttatttt 
      tttctattg atagttgga tataatcaa atatataag 
      ctggcgctt ttttaaata aaatataac aaatgca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY