| General information | |||
| TC033978
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 4139140..4161686 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC033978 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00973 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 100.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
- >TC033978 RA
MKVKDVERT ANVAWSPKN QSPILLAAG TAAQQLDAS FSTNATLEI YSLNLGELG PDMVLKAAT PSEHRFHKI IWGAYESHG TIVGGCDGG LIQIYSASK LMNGENGLI CRQNKHTGP VHALDFNPF QQNLFATGA GDSEIFIWD LNNTNTPMS PGSKSQPLE DVLAISWNR QVQHILAST FASKCVIWD LRKNEPIIK LTDTVSRVR WKMIAWHPE VATQLCLAS EEDHSPIIQ LWDLRFATS PLKTLENHQ RGVLSVAWC ADDPDLLVS CGKDNRIIA WNPNSNEPN GEVLSEIAK TNQWNFDVQ WCPRNPAII ACPSFDGHV SVYSLMGGK TQQIQTTNK IADSFPGMD GYVQAPVLQ QSSAPVSVD LSRPPKWLK RPVGARFGF GGKLISFDN TKPDVDQIS HRTVQISQV ITEPELVQK STELEQALE YGNFTEYCS RKAETTNDQ HTKYIWHFL RANFEENPR AELLNLLGY RIEDVNNKL NQFVHKNKV DGVSERLED LDPFDAIVD NKTVTKPFK IKTGDDTEG LITQALLLG NVEAAVELC LEAKRFADA IIIAMTGGS DLLARTQHA YLEQCEGYV SSLISALVS EDWASVINN CDLSSWKEA LAATLTHST DEALPLLCE QLGNRLSHG DAKLKQDSQ LCYICAGSF DNLVNSWSG NAQNSTSDL QDLVELVVF LQKAVERQG RQVQISGAL ADLLGRYAS ILASQGNLS TALSYLGSS QDEKVAALR ERLYISLGQ KPGYGAQRQ AQSRRLSQR QSFSSYPNM GAQFNSGLP NAAVPVSQP WQPQPQMGG FNQQPPART FSPAPPSQP TRPPSVGSN HGGSGTGLP SRKYVLDPS VTSNPYGAP TRNMYAPQP VSNFQNNNY GAPSPITPL VPSNNNYVP NPVPMVPNP PLNPTPLVP NIQPPPPMT SEMGQPATE KYFPSSAPG WNDPPPLNK PLRPQPKPE ASVPSQPIT HPLYGSAPT NNGYGTEPQ MFQPQPTNN FAPPNNFTP PTTFNPTGF KSNAVNFNQ VQNQTPVVP EKPQPIQKP PIPEEHIHM QTVFDELKY QCSCAANNP QTKRKLEDV GRKLESLYD LLREQKLSP NTLGALHQM VQLVQNGDY TGGLNLHTQ LVSGPDFAQ IASFMPGLK VLLQCALQL QVYLR
- >TC033978 RA
atgaaagtg aaagacgtg gagcgcacc gccaacgta gcgtggtcc cccaagaat cagagcccc attttgtta gcagccggg acagccgcc caacagctc gacgcctcc ttcagcacc aatgccacc ttggagatc tattccctc aacttgggg gagttgggt cccgatatg gtgctcaag gctgcgacc ccgagcgag caccgcttt cataaaatc atctggggg gcttacgag agtcatggg accatcgtg ggtgggtgt gatgggggc ctgatccag atttacagc gcttccaag ttgatgaat ggggagaat gggctgatt tgtcggcag aataagcac acagggcct gtgcatgcg cttgacttt aaccccttt cagcagaat ttgttcgca acgggggcc ggggattcc gagattttt atctgggat ttgaataac accaacacg cccatgagt cctgggtcc aagtcgcag ccgctggag gacgttctg gcgatttcg tggaatagg caagtgcag catatttta gcgtcgact tttgcctcc aagtgtgtc atttgggat ttgaggaag aacgaaccg attattaaa ttaacggac actgtctcg agggttagg tggaaaatg attgcatgg catccggaa gtggccacg cagttgtgt ttagcttct gaggaggat cactctccc attatccaa ttatgggac ctccgtttt gccacatcc ccccttaaa acactcgag aatcaccaa agaggcgta ttatcagtg gcgtggtgt gccgatgat cccgattta ttagtcagt tgtggtaaa gataatcgt attattgcc tggaatcca aattcaaat gaaccaaac ggagaggtt ctctccgaa attgcaaaa acgaatcaa tggaatttt gatgtacag tggtgtcct agaaatcca gccattatt gcatgtccc agttttgat ggacatgtt tctgtttat tctttaatg gggggcaag acccaacag atacaaacc acgaataaa attgctgat agttttccg ggaatggat ggatatgtt caagctcct gttctacag caaagctcg gcaccagtc agtgtggat ttaagtaga ccaccaaag tggcttaag agaccagtt ggagccaga tttgggttt ggtggcaaa ttaatttcg ttcgataac actaaaccc gacgttgat caaatcagc caccgaacg gttcaaatc agtcaagtt ataaccgaa ccagagcta gtccagaaa tcaaccgaa ctggaacaa gctctcgaa tacggcaac ttcactgaa tactgttcc cgcaaagcc gaaaccaca aacgatcaa cacaccaaa tacatttgg cactttctg cgtgccaac ttcgaggaa aaccctcgc gcggaacta ttgaattta ttgggctat cgcatcgaa gacgtcaat aacaaactc aaccaattc gtccacaaa aacaaagtt gacggagta tcggaacgt ttagaggac ttggatcca tttgacgca attgtcgat aataaaaca gtgacaaaa ccgttcaaa atcaaaact ggtgacgac accgaaggc ttaatcact caagcgctg cttttgggg aacgtcgaa gcggcggtt gagttgtgt ttggaggcg aagcgcttc gccgacgct atcattatt gcaatgact gggggctcg gatttattg gctcggacc caacacgcg tacttggag caatgcgag ggctatgtt tcgtcgctg atttcggct ttggtgtcc gaagactgg gcttctgtt atcaataac tgcgacctg agcagttgg aaggaggca cttgcggcc actctgacg cactccacc gacgaggcg ttgcccctt ctgtgcgaa cagctcgga aatcgcctc agccacggc gacgccaaa ctcaaacaa gactcccag ctgtgctac atctgcgct gggagtttc gacaatctg gtcaactcc tggagcggc aatgcgcag aattccacc agcgatttg caggatttg gtcgagttg gtggtgttt ttgcaaaag gcggtggaa aggcaaggg cggcaggtg cagatttcg ggcgctttg gccgatttg ctgggcagg tatgcgtcg attttggcc tcgcagggg aatttgtcg acggcgttg agttacttg gggagttcg caagacgag aaggttgcg gcgttgagg gagcgcttg tatatcagt ttggggcag aagccgggg tatggggcg cagagacag gcacagagt cggaggttg agtcagaga cagtcgttt tcgagttat ccgaacatg ggggcacag tttaattca ggactgccg aatgcagcg gttccggtg tctcagccg tggcaaccg cagccccaa atggggggt tttaatcaa caaccaccg gcgaggacg ttttcgccg gcgccgccg agtcagccg acgaggccc ccaagtgtg ggatctaac catggtggt tctggcacg ggcttgcct tcgcgtaaa tacgttctg gacccctct gtaacctcc aacccctac ggagcgcct acgaggaac atgtacgcc ccccaacct gtctccaac ttccagaac aataactat ggggcccca tcaccaata acacccttg gttccttcc aataataat tacgtaccc aatcctgta ccaatggta cccaatcca ccactcaat cccactcct ttggtacca aatatccaa ccaccgccg cctatgacg agcgagatg ggccaacct gccacagaa aaatacttt ccttctagt gcccccggc tggaacgat ccacctcca ctaaacaaa ccattgaga ccacagccc aaaccagag gcatccgtc ccatctcaa cccataacg cacccattg tatgggtcg gcgcccacc aacaacggt tacggaacc gaaccacaa atgttccaa ccacagcca actaataat tttgcccca cctaacaat ttcacaccg cctactacg ttcaatccg actggtttt aagtcaaat gcggtcaat tttaaccag gtccaaaac caaactcct gtggttcca gagaaacca caaccaatt caaaaacca ccaattccc gaagaacac atacacatg caaaccgtt tttgacgag ttgaaatat cagtgcagt tgtgcggct aataatccg caaaccaag cgaaaattg gaggatgtt ggccgaaag ttagagtcg ttgtatgat ttgttaagg gaacagaaa ctgtcaccc aacacttta ggagcgtta catcaaatg gtacagttg gtgcaaaac ggagattat acaggagga ctgaattta cacactcag ctagtgtca gggcctgat ttcgctcaa attgccagt tttatgccc gggcttaaa gttttgtta cagtgcgct ttacaattg caagtttat ttaagataa
- >TC033978 RA
tgggaaagt gaaagaatg tatatcaaa ttttaaatt ttttgaaac atcgataaa acagaatcc tgcgttacc gaccagtct ttccaatta aaaattccc aaaatttga ttaatttgt ctaaaatcg tatcgctaa tttaattat tcgctttac caccttacg ttagtgtct cataaattt tattgcccc ttgataaca actaaacca atgaaaaaa taagttcgg ttaggtcgt gttgacgtg ttcctgtca tctgattgt cagtcgtaa aataagtta agccacgtt ggatgtctt cccgtgtgt tttacgtta aataagtgc ttaaccatg aaagtgaaa gacgtggag cgcaccgcc aacgtagcg tggtccccc aagaatcag agccccatt ttgttagca gccgggaca gccgcccaa cagctcgac gcctccttc agcaccaat gccaccttg gagatctat tccctcaac ttgggggag ttgggtccc gatatggtg ctcaaggct gcgaccccg agcgagcac cgctttcat aaaatcatc tggggggct tacgagagt catgggacc atcgtgggt gggtgtgat gggggcctg atccagatt tacagcgct tccaagttg atgaatggg gagaatggg ctgatttgt cggcagaat aagcacaca gggcctgtg catgcgctt gactttaac ccctttcag cagaatttg ttcgcaacg ggggccggg gattccgag atttttatc tgggatttg aataacacc aacacgccc atgagtcct gggtccaag tcgcagccg ctggaggac gttctggcg atttcgtgg aataggcaa gtgcagcat attttagcg tcgactttt gcctccaag tgtgtcatt tgggatttg aggaagaac gaaccgatt attaaatta acggacact gtctcgagg gttaggtgg aaaatgatt gcatggcat ccggaagtg gccacgcag ttgtgttta gcttctgag gaggatcac tctcccatt atccaatta tgggacctc cgttttgcc acatccccc cttaaaaca ctcgagaat caccaaaga ggcgtatta tcagtggcg tggtgtgcc gatgatccc gatttatta gtcagttgt ggtaaagat aatcgtatt attgcctgg aatccaaat tcaaatgaa ccaaacgga gaggttctc tccgaaatt gcaaaaacg aatcaatgg aattttgat gtacagtgg tgtcctaga aatccagcc attattgca tgtcccagt tttgatgga catgtttct gtttattct ttaatgggg ggcaagacc caacagata caaaccacg aataaaatt gctgatagt tttccggga atggatgga tatgttcaa gctcctgtt ctacagcaa agctcggca ccagtcagt gtggattta agtagacca ccaaagtgg cttaagaga ccagttgga gccagattt gggtttggt ggcaaatta atttcgttc gataacact aaacccgac gttgatcaa atcagccac cgaacggtt caaatcagt caagttata accgaacca gagctagtc cagaaatca accgaactg gaacaagct ctcgaatac ggcaacttc actgaatac tgttcccgc aaagccgaa accacaaac gatcaacac accaaatac atttggcac tttctgcgt gccaacttc gaggaaaac cctcgcgcg gaactattg aatttattg ggctatcgc atcgaagac gtcaataac aaactcaac caattcgtc cacaaaaac aaagttgac ggagtatcg gaacgttta gaggacttg gatccattt gacgcaatt gtcgataat aaaacagtg acaaaaccg ttcaaaatc aaaactggt gacgacacc gaaggctta atcactcaa gcgctgctt ttggggaac gtcgaagcg gcggttgag ttgtgtttg gaggcgaag cgcttcgcc gacgctatc attattgca atgactggg ggctcggat ttattggct cggacccaa cacgcgtac ttggagcaa tgcgagggc tatgtttcg tcgctgatt tcggctttg gtgtccgaa gactgggct tctgttatc aataactgc gacctgagc agttggaag gaggcactt gcggccact ctgacgcac tccaccgac gaggcgttg ccccttctg tgcgaacag ctcggaaat cgcctcagc cacggcgac gccaaactc aaacaagac tcccagctg tgctacatc tgcgctggg agtttcgac aatctggtc aactcctgg agcggcaat gcgcagaat tccaccagc gatttgcag gatttggtc gagttggtg gtgtttttg caaaaggcg gtggaaagg caagggcgg caggtgcag atttcgggc gctttggcc gatttgctg ggcaggtat gcgtcgatt ttggcctcg caggggaat ttgtcgacg gcgttgagt tacttgggg agttcgcaa gacgagaag gttgcggcg ttgagggag cgcttgtat atcagtttg gggcagaag ccggggtat ggggcgcag agacaggca cagagtcgg aggttgagt cagagacag tcgttttcg agttatccg aacatgggg gcacagttt aattcagga ctgccgaat gcagcggtt ccggtgtct cagccgtgg caaccgcag ccccaaatg gggggtttt aatcaacaa ccaccggcg aggacgttt tcgccggcg ccgccgagt cagccgacg aggccccca agtgtggga tctaaccat ggtggttct ggcacgggc ttgccttcg cgtaaatac gttctggac ccctctgta acctccaac ccctacgga gcgcctacg aggaacatg tacgccccc caacctgtc tccaacttc cagaacaat aactatggg gccccatca ccaataaca cccttggtt ccttccaat aataattac gtacccaat cctgtacca atggtaccc aatccacca ctcaatccc actcctttg gtaccaaat atccaacca ccgccgcct atgacgagc gagatgggc caacctgcc acagaaaaa tactttcct tctagtgcc cccggctgg aacgatcca cctccacta aacaaacca ttgagacca cagcccaaa ccagaggca tccgtccca tctcaaccc ataacgcac ccattgtat gggtcggcg cccaccaac aacggttac ggaaccgaa ccacaaatg ttccaacca cagccaact aataatttt gccccacct aacaatttc acaccgcct actacgttc aatccgact ggttttaag tcaaatgcg gtcaatttt aaccaggtc caaaaccaa actcctgtg gttccagag aaaccacaa ccaattcaa aaaccacca attcccgaa gaacacata cacatgcaa accgttttt gacgagttg aaatatcag tgcagttgt gcggctaat aatccgcaa accaagcga aaattggag gatgttggc cgaaagtta gagtcgttg tatgatttg ttaagggaa cagaaactg tcacccaac actttagga gcgttacat caaatggta cagttggtg caaaacgga gattataca ggaggactg aatttacac actcagcta gtgtcaggg cctgatttc gctcaaatt gccagtttt atgcccggg cttaaagtt ttgttacag tgcgcttta caattgcaa gtttattta agataaggt ctaaccatg tattatttt gttttagat gtttatttt tttctattg atagttgga tataatcaa atatataag ctggcgctt ttttaaata aaatataac aaatgca
