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Gene information
General information
TC005986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 4178198..4182331
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005986
    iBeetle screen iB_00971

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005986 RA
      MESFVLILV ISLFAEIRC KMEPIVLVH GGAGDIPDS 
      RVSLKITGV KKAASLGYK VLKNGGSVV EAVEEAVKS 
      MEDDEAFNA GYGSVLNLD GDVEMDASI MLGSNLLSG 
      GVTVVKDIA HPISLARLV MEKTPHFLL AGAGANRFA 
      KEQGVPSVP PGSLVSKYA KEALEHFMK YGDNGTEMS 
      GVGEVGTVG AVAIDSKGR LAAATSTGG ITGKMAGRS 
      SDTCIIGSG TYADDNVGA VSTTGHGES IARFCLAHA 
      IIYEMRNGS SASEATAKA LKEMKGRLP NTAGAITIS 
      KSGELGIDF TSRRMAWAY QVKDEMHFG IEHGQHTKE 
      KV
    CDS sequence
    • >TC005986 RA
      atggaatct ttcgttcta attttagtt atatcactg 
      tttgcagag attaggtgt aaaatggag ccgattgtc 
      ctcgtccac ggtggtgca ggtgatatt cccgattcc 
      agggtttcc ctcaaaatc acaggagtg aaaaaagcc 
      gcatctttg ggttacaaa gttttgaaa aatggcggg 
      agcgttgtc gaggctgtg gaagaagct gtcaagtcg 
      atggaagat gacgaagct tttaatgcc ggatatggg 
      tccgttttg aatctggac ggcgacgtg gaaatggac 
      gcctccatc atgcttggt tcaaacctc ctctccggg 
      ggcgtaacc gtcgtcaaa gacatcgcc caccccatt 
      tccctggcc cgccttgtc atggaaaaa acgccccac 
      tttctcctc gccggagct ggggccaac cgctttgcc 
      aaagaacaa ggcgttccc tccgtcccc cccggcagt 
      ctagtttca aaatacgca aaagaagcg ttggaacac 
      ttcatgaaa tacggcgat aacggcacc gaaatgagt 
      ggtgtggga gaggtgggg acagttggg gcggtggca 
      atcgattcg aaaggacga ttggctgcg gccacctcc 
      acgggcggg atcaccggc aaaatggcg gggcgaagt 
      agcgatacc tgtatcatt gggagtggc acttatgct 
      gacgataac gtgggggct gtctcaaca actggacat 
      ggggaaagt atcgccaga ttttgtctc gcccatgcc 
      attatctac gaaatgagg aatgggtca agtgccagt 
      gaggccacg gctaaggca ttgaaggaa atgaagggg 
      cggttgccc aacactgct ggggctatc accatttcg 
      aagagtggg gagttgggg attgatttt acgagtagg 
      agaatggcg tgggcatac caggtcaag gacgagatg 
      cattttggc atcgaacac gggcaacac accaaagaa 
      aaggtttaa
    mRNA sequence
    • >TC005986 RA
      actaaacca gttctcctc agatttctt cccgagacc 
      ttgcctact gctcttaag actcacaat ttgtagtgc 
      catggaatc tttcgttct aattttagt tatatcact 
      gtttgcaga gattaggtg taaaatgga gccgattgt 
      cctcgtcca cggtggtgc aggtgatat tcccgattc 
      cagggtttc cctcaaaat cacaggagt gaaaaaagc 
      cgcatcttt gggttacaa agttttgaa aaatggcgg 
      gagcgttgt cgaggctgt ggaagaagc tgtcaagtc 
      gatggaaga tgacgaagc ttttaatgc cggatatgg 
      gtccgtttt gaatctgga cggcgacgt ggaaatgga 
      cgcctccat catgcttgg ttcaaacct cctctccgg 
      gggcgtaac cgtcgtcaa agacatcgc ccaccccat 
      ttccctggc ccgccttgt catggaaaa aacgcccca 
      ctttctcct cgccggagc tggggccaa ccgctttgc 
      caaagaaca aggcgttcc ctccgtccc ccccggcag 
      tctagtttc aaaatacgc aaaagaagc gttggaaca 
      cttcatgaa atacggcga taacggcac cgaaatgag 
      tggtgtggg agaggtggg gacagttgg ggcggtggc 
      aatcgattc gaaaggacg attggctgc ggccacctc 
      cacgggcgg gatcaccgg caaaatggc ggggcgaag 
      tagcgatac ctgtatcat tgggagtgg cacttatgc 
      tgacgataa cgtgggggc tgtctcaac aactggaca 
      tggggaaag tatcgccag attttgtct cgcccatgc 
      cattatcta cgaaatgag gaatgggtc aagtgccag 
      tgaggccac ggctaaggc attgaagga aatgaaggg 
      gcggttgcc caacactgc tggggctat caccatttc 
      gaagagtgg ggagttggg gattgattt tacgagtag 
      gagaatggc gtgggcata ccaggtcaa ggacgagat 
      gcattttgg catcgaaca cgggcaaca caccaaaga 
      aaaggttta attgttaaa acaatctac ttgtattgt 
      atttagact agaaataaa aattgttta aaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY