| General information | |||
| TC005952 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 4893520..4912504 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC005952 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_00967 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC005952 RA
MFGRRRRRS STIRVMQLF HELKQQFPT VPDHVVNAC IASYTHSNS PHKDATIRE LLEAAAQNQ EPMGRSPPT SQPSPTPSQ HPPPATSER TEMNASAGD INQNVVKYV KKHESVSVC PESKSQFFV KRPDTLELQ PSGKCKDVH KLLNSEHVS DNKPPRSPK RGTRRTPEA KRLQDEGKK ETASTPTQT TDTLLGGNS VNLSLNVNV DVLPSPTRR KSVLQVTPQ QPWLEEPLS PRSYTSVNL TLRPPSSEP QAPIDITSQ NSSLTYSTR SYDSQKGLQ SRLQITVGP GGGSVSSTR ARPRSSYHP EQAVEEVVP YRAGSLTNL AASSEPPVI LKQQARIDR LKIELRTEK AKLEVMQKE VIDLEGRNR PLAIQPTDA EVEKQLRRE IKHLQYQCE QLALEVDKN SDLQEDFYH NIYTGQRGP LNVEGRRFT QPNAFQDNE GPKWNCHLC TFLNHPDLD KCEQCEMPR ILHGKSPSN PNINYQNPT NYNLVPSYS LPNFHEMSR GPVVVNRPI GFLLNENND ITRPN
- >TC005952 RB
MFGRRRRRS STIRVMQLF HELKQQFPT VPDHVVNAC IASYTHSNS PHKDATIRE LLEAAAQNQ EPMGRSPPT SQPSPTPSQ HPPPATSER TEMNASAGD INQNVVKYV KKHESVSVC PESKSQFFV KRPDTLELQ PSGKCKDVH KLLNSEHVS DNKPPRSPK RGTRRTPEA KRLQDEGKK ETASTPTQT TDTLLGGNS VNLSLNVNV DVLPSPTRR KSVLQVTPQ QPWLEEPLS PRSYTSVNL TLRPPSSEP QAPIDITSQ NSSLTYSTR SYDSQKGLQ SRLQITVGP GGGSVSSTR ARPRSSYHP EQAVEEVVP YRAGSLTNL AASSEPPVI LKQQARIDR LKIELRTEK AKLEVMQKE VIDLEGRNR PLAIQPTDA EVEKQLRRE IKHLQYQCE QLALEVDKN SDLQEDFYH NIYTGQRGP LNVEGRRFT QPNAFQDNE GPKWNCHLC TFLNHPDLD KCEQCEMPR ILHVSAAPG DNIHIHVTP RLSRRVVHS WVL
- >TC005952 RA
atgtttggc agaaggaga cgcaggtcg tccaccatc cgtgtgatg cagctattc catgaacta aaacagcaa tttcccacc gttcccgac cacgtcgta aacgcctgt atcgcctcc tacacccat tcaaactcc cctcacaaa gacgccacc atccgggag ttgctggaa gctgccgcc caaaaccaa gagccgatg ggccgttct cctccaaca tctcaaccc tcaccaact ccctcccaa caccctcca ccagcaacc agcgaacga accgaaatg aacgcgagt gcaggtgac ataaatcaa aatgtagtc aaatacgtt aaaaagcac gaatcggtg agtgtgtgc ccggaatcg aaaagtcaa ttcttcgtc aaaagaccg gacactttg gaactccaa ccgtcggga aaatgcaaa gacgttcac aaactctta aactcggaa catgtgagt gataataag ccgccacgg tccccaaaa aggggcacc cggaggact cctgaggcg aaaagactg caagatgaa ggcaagaag gaaacagcg tcgactcca acccaaacc accgacact ctccttgga gggaacagt gtcaacctc tccctcaac gttaacgtg gacgtcctc ccgagccca acccgtcgc aaatccgtc ctccaagtc accccacag cagccctgg ttagaggag cccctcagt ccaaggagt tacacaagt gttaatcta actctgcgg ccaccttcg tccgaaccc caagccccc atagacata acttctcaa aactcgagt ctgacttac tccacacgt agttatgac tcgcaaaag gggctccag agccggctt cagattact gtggggcct gggggtggg agtgtttct tcaacaagg gcgaggccg aggagtagt taccaccca gagcaagct gtggaggag gtggtaccg tacagggcc ggctctttg actaatctt gcggcaagc agtgaaccc cccgtgata ttaaaacaa caggcccga atcgatcgt ctcaaaatc gagttaaga accgaaaaa gccaagctc gaagtgatg caaaaagaa gtgatcgat ttggagggc cgtaaccgc ccccttgcc atccagccc actgatgca gaggtggag aaacaactg cgacgggaa atcaagcat ttgcagtat cagtgcgag cagctagct ctcgaagtg gacaaaaat agtgatttg caggaggat ttctaccat aacatttac acgggtcag aggggccct ctgaacgta gaagggcgc cgcttcacc caaccaaat gcgtttcaa gacaacgaa ggtccgaaa tggaattgt cacttgtgt acattcctt aatcatcca gacttggat aaatgtgaa cagtgcgaa atgccgagg atattacat ggtaaaagt ccctcaaat ccgaatatt aattaccaa aatcctact aattacaat cttgtgcct tcctatagt cttccgaat tttcacgaa atgagtaga ggaccggtt gtggtcaac agaccaatc ggttttctc ctcaatgaa aataatgat atcacaagg cctaattga
- >TC005952 RB
atgtttggc agaaggaga cgcaggtcg tccaccatc cgtgtgatg cagctattc catgaacta aaacagcaa tttcccacc gttcccgac cacgtcgta aacgcctgt atcgcctcc tacacccat tcaaactcc cctcacaaa gacgccacc atccgggag ttgctggaa gctgccgcc caaaaccaa gagccgatg ggccgttct cctccaaca tctcaaccc tcaccaact ccctcccaa caccctcca ccagcaacc agcgaacga accgaaatg aacgcgagt gcaggtgac ataaatcaa aatgtagtc aaatacgtt aaaaagcac gaatcggtg agtgtgtgc ccggaatcg aaaagtcaa ttcttcgtc aaaagaccg gacactttg gaactccaa ccgtcggga aaatgcaaa gacgttcac aaactctta aactcggaa catgtgagt gataataag ccgccacgg tccccaaaa aggggcacc cggaggact cctgaggcg aaaagactg caagatgaa ggcaagaag gaaacagcg tcgactcca acccaaacc accgacact ctccttgga gggaacagt gtcaacctc tccctcaac gttaacgtg gacgtcctc ccgagccca acccgtcgc aaatccgtc ctccaagtc accccacag cagccctgg ttagaggag cccctcagt ccaaggagt tacacaagt gttaatcta actctgcgg ccaccttcg tccgaaccc caagccccc atagacata acttctcaa aactcgagt ctgacttac tccacacgt agttatgac tcgcaaaag gggctccag agccggctt cagattact gtggggcct gggggtggg agtgtttct tcaacaagg gcgaggccg aggagtagt taccaccca gagcaagct gtggaggag gtggtaccg tacagggcc ggctctttg actaatctt gcggcaagc agtgaaccc cccgtgata ttaaaacaa caggcccga atcgatcgt ctcaaaatc gagttaaga accgaaaaa gccaagctc gaagtgatg caaaaagaa gtgatcgat ttggagggc cgtaaccgc ccccttgcc atccagccc actgatgca gaggtggag aaacaactg cgacgggaa atcaagcat ttgcagtat cagtgcgag cagctagct ctcgaagtg gacaaaaat agtgatttg caggaggat ttctaccat aacatttac acgggtcag aggggccct ctgaacgta gaagggcgc cgcttcacc caaccaaat gcgtttcaa gacaacgaa ggtccgaaa tggaattgt cacttgtgt acattcctt aatcatcca gacttggat aaatgtgaa cagtgcgaa atgccgagg atattacat gtttctgca gctccaggt gataatatt cacattcat gtaactcca agactatct agacgtgtt gtacactct tgggtttta taa
- >TC005952 RA
cacctgtcc gtggcgtcg ccactgtcg cactttcat ttcacgtga cagacagtt ctatttgtg tttcaccaa cttcagtct ggcacaaca tacgatccg aaggttgca ttgtaaatt cgccaagtt ctccctcaa accctcaaa aattcataa aacagccgt ccctcaacc cttcctctc gagtgtgca gtgctagtg agtgcgctt cgaccatgt ttcccccgc tggaacccc tcaaacagg cagttgtta tgtgatttc gctacccca taatggccg gccatgttt ggcagaagg agacgcagg tcgtccacc atccgtgtg atgcagcta ttccatgaa ctaaaacag caatttccc accgttccc gaccacgtc gtaaacgcc tgtatcgcc tcctacacc cattcaaac tcccctcac aaagacgcc accatccgg gagttgctg gaagctgcc gcccaaaac caagagccg atgggccgt tctcctcca acatctcaa ccctcacca actccctcc caacaccct ccaccagca accagcgaa cgaaccgaa atgaacgcg agtgcaggt gacataaat caaaatgta gtcaaatac gttaaaaag cacgaatcg gtgagtgtg tgcccggaa tcgaaaagt caattcttc gtcaaaaga ccggacact ttggaactc caaccgtcg ggaaaatgc aaagacgtt cacaaactc ttaaactcg gaacatgtg agtgataat aagccgcca cggtcccca aaaaggggc acccggagg actcctgag gcgaaaaga ctgcaagat gaaggcaag aaggaaaca gcgtcgact ccaacccaa accaccgac actctcctt ggagggaac agtgtcaac ctctccctc aacgttaac gtggacgtc ctcccgagc ccaacccgt cgcaaatcc gtcctccaa gtcacccca cagcagccc tggttagag gagcccctc agtccaagg agttacaca agtgttaat ctaactctg cggccacct tcgtccgaa ccccaagcc cccatagac ataacttct caaaactcg agtctgact tactccaca cgtagttat gactcgcaa aaggggctc cagagccgg cttcagatt actgtgggg cctgggggt gggagtgtt tcttcaaca agggcgagg ccgaggagt agttaccac ccagagcaa gctgtggag gaggtggta ccgtacagg gccggctct ttgactaat cttgcggca agcagtgaa ccccccgtg atattaaaa caacaggcc cgaatcgat cgtctcaaa atcgagtta agaaccgaa aaagccaag ctcgaagtg atgcaaaaa gaagtgatc gatttggag ggccgtaac cgccccctt gccatccag cccactgat gcagaggtg gagaaacaa ctgcgacgg gaaatcaag catttgcag tatcagtgc gagcagcta gctctcgaa gtggacaaa aatagtgat ttgcaggag gatttctac cataacatt tacacgggt cagaggggc cctctgaac gtagaaggg cgccgcttc acccaacca aatgcgttt caagacaac gaaggtccg aaatggaat tgtcacttg tgtacattc cttaatcat ccagacttg gataaatgt gaacagtgc gaaatgccg aggatatta catggtaaa agtccctca aatccgaat attaattac caaaatcct actaattac aatcttgtg ccttcctat agtcttccg aattttcac gaaatgagt agaggaccg gttgtggtc aacagacca atcggtttt ctcctcaat gaaaataat gatatcaca aggcctaat tgactggct gtgattgta ctttttcaa ctgtgatag tttgaaaaa ttgttattt tagtacctt atgtatgtt tgttgaagc aataaagtt attgtattg tt
- >TC005952 RB
cacctgtcc gtggcgtcg ccactgtcg cactttcat ttcacgtga cagacagtt ctatttgtg tttcaccaa cttcagtct ggcacaaca tacgatccg aaggttgca ttgtaaatt cgccaagtt ctccctcaa accctcaaa aattcataa aacagccgt ccctcaacc cttcctctc gagtgtgca gtgctagtg agtgcgctt cgaccatgt ttcccccgc tggaacccc tcaaacagg cagttgtta tgtgatttc gctacccca taatggccg gccatgttt ggcagaagg agacgcagg tcgtccacc atccgtgtg atgcagcta ttccatgaa ctaaaacag caatttccc accgttccc gaccacgtc gtaaacgcc tgtatcgcc tcctacacc cattcaaac tcccctcac aaagacgcc accatccgg gagttgctg gaagctgcc gcccaaaac caagagccg atgggccgt tctcctcca acatctcaa ccctcacca actccctcc caacaccct ccaccagca accagcgaa cgaaccgaa atgaacgcg agtgcaggt gacataaat caaaatgta gtcaaatac gttaaaaag cacgaatcg gtgagtgtg tgcccggaa tcgaaaagt caattcttc gtcaaaaga ccggacact ttggaactc caaccgtcg ggaaaatgc aaagacgtt cacaaactc ttaaactcg gaacatgtg agtgataat aagccgcca cggtcccca aaaaggggc acccggagg actcctgag gcgaaaaga ctgcaagat gaaggcaag aaggaaaca gcgtcgact ccaacccaa accaccgac actctcctt ggagggaac agtgtcaac ctctccctc aacgttaac gtggacgtc ctcccgagc ccaacccgt cgcaaatcc gtcctccaa gtcacccca cagcagccc tggttagag gagcccctc agtccaagg agttacaca agtgttaat ctaactctg cggccacct tcgtccgaa ccccaagcc cccatagac ataacttct caaaactcg agtctgact tactccaca cgtagttat gactcgcaa aaggggctc cagagccgg cttcagatt actgtgggg cctgggggt gggagtgtt tcttcaaca agggcgagg ccgaggagt agttaccac ccagagcaa gctgtggag gaggtggta ccgtacagg gccggctct ttgactaat cttgcggca agcagtgaa ccccccgtg atattaaaa caacaggcc cgaatcgat cgtctcaaa atcgagtta agaaccgaa aaagccaag ctcgaagtg atgcaaaaa gaagtgatc gatttggag ggccgtaac cgccccctt gccatccag cccactgat gcagaggtg gagaaacaa ctgcgacgg gaaatcaag catttgcag tatcagtgc gagcagcta gctctcgaa gtggacaaa aatagtgat ttgcaggag gatttctac cataacatt tacacgggt cagaggggc cctctgaac gtagaaggg cgccgcttc acccaacca aatgcgttt caagacaac gaaggtccg aaatggaat tgtcacttg tgtacattc cttaatcat ccagacttg gataaatgt gaacagtgc gaaatgccg aggatatta catgtttct gcagctcca ggtgataat attcacatt catgtaact ccaagacta tctagacgt gttgtacac tcttgggtt ttataatag gatcaggta attaagaga atgacgaac tgctatttt tttattttc tttacaaat ttgttgtca aacaaataa ttgctcatt taaatacac aatgtgtaa atga
