Status
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Gene information
General information
TC005949 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5015642..5035537
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005949
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00966

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax segment number increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005949 RA
      MTYIQGNFC NSMVDADLY LLQIACTRM NYNDFISLI 
      IDKFHVTES LSLAERPPQ NPYLKPEHN NRMLESFLT 
      FLATLVTVR TNIGLSETE LNRLEMATL LCMSDRTHS 
      QLMELMPER CGTSQTRDF ETLLAELAD YKPPVLDPS 
      GGNLQQGMY SAKPHVWEN LYDPIHVLL RAVHRRDFH 
      TSMDRYTDY VKDANKLKG GISPWLPFR EPTSCSKAY 
      DDPRLILKS RVFHAVILV ILQKAVRGQ IPEDVTALI 
      IFLLDQAVT ISERKEEQA EPKFYHHQS KPLNDMNFD 
      NWYSSDCIF ENLATPIAK IVLTPEPEV SPMTYSSDS 
      DIEWENSEV DTEMSDVNP DGNNILMLQ DSAGWDQSG 
      TDLMVFVPQ DSSPGFEPT NPVLALPPS TSMDVDNMT 
      VAVTTRSVV PAHSEPPAL PAITAGNEV ARPWTGNEL 
      VPSTSSASG HRRPYRRRP TLEGGGATP PTVELNESI 
      ISLLLKLHS QLSGVPDSF KGEEEDDEG RGLIGDGPF 
      FIGRLLRKI SRLDEECRR CVLEARIKL WPSQEERDE 
      AKRQRENRE KEERRRRAK ERQQKLMAE FASKQKQFM 
      EAMETDDNV LNSDEELFV SKQEYDCVI CNQATPSTE 
      EKPMGLVVW IQATSVIGH RRRHGQSVL PTCDDERTS 
      LRRDDTLSA EFDRRLEEL NQYFDPKSW YLSVNVGWD 
      GGVHVQTCG HHLHLDCLN SYLQTLRSQ QRGEYFCPL 
      CRQLANSVL PLAPQLGDC AQMVRSNPS SMSQILDEL 
      SDFLRENDQ KPSNTSMAE AIGKIMEVM TSSTQLKPM 
      LRSKSDRPI PQSLFLFVT SIARTNLET ELSQRGGTL 
      VMSRDPPNP LLPKRDCIV PLLHVLSVH ARVARVLAM 
      WPAWMTFQQ LCGLPPKPP SDTALAPVE NEVPLLLRD 
      PVALLLQFI LLLPLHLDQ NYFITTVQM VYNLLYYQV 
      VAQISCGLT GAERARATA GANEGKICT LTDALALIN 
      RCLGHTALY MEDDEECTE KPQVNMVAL ELQVQKLCL 
      PYLRIAALL KYHIYQQAL PDVRAPETE FVCLVHYLE 
      LVTEQVTWD CNAAAALNW PIETQMYTA SPQSWCNQY 
      AVFVAKSQI AARSFLVDQ HITWHPPRL LKLPREYEK 
      IFTYYHKRP CSQCQSVPQ ETGICLMCG TIVCLKQSC 
      CKQQNICEA TAHASECGA GTGVFLVVT STYIIVIRR 
      HRACLWGSL YLDDFEEED RDLKRGKPL YLSRERYQL 
      LEQQWLAHR FDHIEKTWI QHGNAL
    CDS sequence
    • >TC005949 RA
      atgacttac atccaagga aatttctgt aattcgatg 
      gtggacgcc gatttgtat ctcctccaa atcgcatgt 
      acgcgaatg aattacaac gacttcatc tctcttatt 
      atcgacaag ttccacgta accgaatct ctgagtctt 
      gctgaacga cctcctcaa aacccctac cttaaaccc 
      gaacataac aatcgaatg ttggagagt ttcttgacg 
      tttttggcc actcttgta acagttcgg acgaatatt 
      ggattgtcc gaaaccgaa ctgaatcgt ctcgaaatg 
      gcaacgctg ttgtgtatg agtgatcgg acgcattcg 
      caactaatg gaattgatg ccggaacgt tgtggtacg 
      tctcaaacg cgcgatttt gagacactt ttggccgaa 
      ctggccgat tataaaccg ccagttttg gatcccagt 
      ggtggtaat ttacagcaa gggatgtat tcggctaag 
      ccacacgtt tgggagaat ttatacgat ccaattcat 
      gtgctgtta cgtgccgta catcgacgt gatttccac 
      acgtcgatg gatcgctac acggattat gtcaaagat 
      gcgaataaa ttgaaaggt gggatcagt ccgtggcta 
      ccattccgc gaacccacc tcatgttcc aaagcctac 
      gacgatccg agactgatt ttaaaatca agggtcttc 
      catgccgtt attttggtc attttacaa aaggccgtt 
      cgaggacaa attcccgaa gatgtaaca gctttaatt 
      attttccta ttggatcaa gcggtgacc attagcgag 
      cgaaaagaa gaacaggcg gagccaaag ttctaccac 
      caccaatca aaaccacta aacgatatg aatttcgac 
      aattggtac tcgtctgat tgcattttt gaaaatttg 
      gccacgccc atcgctaaa attgtgtta accccagaa 
      cctgaagtc tcccccatg acgtatagt agtgatagc 
      gacatcgag tgggaaaat tcggaagtt gatacggaa 
      atgtccgat gttaatccg gatgggaat aatattttg 
      atgctgcaa gattcagcc ggttgggac caatcaggt 
      accgatttg atggtgttt gttccgcaa gactcctcc 
      cccggtttt gagccgacc aatcccgtg ctagctctc 
      ccaccgtcg acgtcaatg gacgtggat aatatgact 
      gttgccgtg acgacgcgt tctgttgtc cccgcccat 
      tcggaaccg cctgcactt ccggcgatt acggccgga 
      aatgaagtt gcgagacct tggactggg aatgagttg 
      gttccgtca acgtcaagc gcctcgggt cataggagg 
      ccgtacagg aggcggccg actttggaa gggggcggg 
      gcgactccg cccactgtt gaactgaac gaaagtata 
      atttcgttg ttgttaaag ctgcattcg caattgtcg 
      ggcgttccg gatagtttt aaaggggag gaggaggat 
      gatgaaggg cgtggtttg attggcgac gggccgttt 
      tttattgga cgtttgttg aggaaaatc tcgcgattg 
      gatgaggag tgtcgaagg tgtgtcttg gaggcgagg 
      ataaagttg tggcccagt caggaagaa cgggacgaa 
      gcgaaacga caaagagag aatcgcgag aaggaggaa 
      agaaggagg agagcaaaa gaaagacaa cagaaatta 
      atggcagag tttgcttca aaacaaaaa cagtttatg 
      gaagcgatg gaaacggat gataacgtt ttgaattcg 
      gacgaggag ttgtttgtg agtaaacag gagtacgat 
      tgtgtgatt tgtaaccaa gccacgccc agtactgag 
      gagaaaccg atggggttg gttgtttgg attcaagcg 
      actagtgtt atcgggcat aggcggaga catggtcag 
      tcagtgttg ccaacttgt gatgatgaa agaacgtca 
      ttgagacga gatgatacg ttgagtgcc gagtttgac 
      cgacggttg gaggaattg aatcaatat tttgatcct 
      aaatcttgg tacttatca gtgaacgtt ggatgggat 
      ggaggtgtt catgtgcaa acttgtggc caccatctt 
      cacctagac tgtcttaat tcgtatttg caaactttg 
      aggtcacaa caacgtggt gagtatttt tgtccgctt 
      tgtcggcaa ttggcgaac tcggtcctg cccctggcg 
      ccccaattg ggcgattgt gcccaaatg gttcgctcg 
      aatccttcc agtatgtcg caaattttg gacgaattg 
      agcgatttt ttgcgtgaa aacgaccaa aaacctagt 
      aatacaagt atggcggaa gcgatcgga aaaataatg 
      gaagttatg acaagtagt acacaattg aaaccgatg 
      cttcgatcg aaaagtgat cgtcctata cctcaaagt 
      ctcttccta tttgtcaca tcaatcgct cggaccaat 
      ctcgagacg gaattgagt caacgaggc gggacttta 
      gtgatgtca cgtgaccca cctaatcca ctcctacca 
      aaacgcgat tgtatagtt cctctactt catgtactt 
      tcggtccac gctcgcgtg gctcgagtg ttggctatg 
      tggccggct tggatgacc ttccagcag ctgtgtggt 
      ttgcccccg aaaccccca agtgatacg gcactggcg 
      ccggtcgaa aacgaagtg ccgctactg ctcagggat 
      ccagtggcg cttcttttg caatttatc ctgcttttg 
      ccgctacat ctagatcaa aattatttc ataacgaca 
      gtccaaatg gtatacaat ttattgtat taccaagtg 
      gtggcgcag atatcctgc ggtttaacc ggagccgag 
      cgcgcccga gccacggcg ggcgctaac gaaggcaaa 
      atttgtacg ttaaccgat gcccttgcc cttatcaat 
      cggtgtttg ggacacact gcgttatac atggaggac 
      gatgaagag tgcaccgaa aaacctcaa gtcaatatg 
      gtggctctt gagctacaa gttcaaaaa ttatgtctg 
      ccttatcta cgaatcgct gctcttctc aaatatcac 
      atctatcag caagccttg cccgatgtg agggcaccc 
      gagaccgaa ttcgtttgt ttggtgcat tatttggaa 
      ttggttact gaacaggtc acgtgggac tgtaatgcc 
      gccgctgct ttgaattgg ccaatcgaa acacagatg 
      tatacagca tcaccacaa tcgtggtgt aaccagtat 
      gcggttttc gttgctaaa agtcagata gcagcacgg 
      tcgttcctt gtggatcaa cacatcacg tggcatccg 
      ccacgccta cttaaattg ccgagggag tacgagaag 
      attttcacc tactatcac aagaggccg tgtagtcaa 
      tgccagtct gtcccgcaa gaaacgggc atttgttta 
      atgtgtggc accatcgtg tgtttgaaa cagtcatgt 
      tgcaaacaa cagaacatc tgtgaggcg actgcgcat 
      gcgtcggag tgtggtgcc ggcaccggt gtttttctc 
      gtagttaca tcgacttat attattgta ataagaagg 
      cacagggcg tgtctttgg ggttcgtta tacttggat 
      gattttgaa gaggaagat cgggatttg aagcgcggg 
      aaaccgttg tatttgagt agggagagg taccaactg 
      ttggagcag caatggcta gcgcatcgg tttgaccac 
      atcgaaaaa acgtggatc cagcacgga aatgctctt 
      taa
    mRNA sequence
    • >TC005949 RA
      caagaacga aatcttttt gagtaaaaa ttcttattt 
      ttttttcag gtggcgttt tacgaaatt ctgaacgac 
      ttgtgggtc cgcaacggt ctccaaatc aagggccaa 
      gccatgact tacatccaa ggaaatttc tgtaattcg 
      atggtggac gccgatttg tatctcctc caaatcgca 
      tgtacgcga atgaattac aacgacttc atctctctt 
      attatcgac aagttccac gtaaccgaa tctctgagt 
      cttgctgaa cgacctcct caaaacccc taccttaaa 
      cccgaacat aacaatcga atgttggag agtttcttg 
      acgtttttg gccactctt gtaacagtt cggacgaat 
      attggattg tccgaaacc gaactgaat cgtctcgaa 
      atggcaacg ctgttgtgt atgagtgat cggacgcat 
      tcgcaacta atggaattg atgccggaa cgttgtggt 
      acgtctcaa acgcgcgat tttgagaca cttttggcc 
      gaactggcc gattataaa ccgccagtt ttggatccc 
      agtggtggt aatttacag caagggatg tattcggct 
      aagccacac gtttgggag aatttatac gatccaatt 
      catgtgctg ttacgtgcc gtacatcga cgtgatttc 
      cacacgtcg atggatcgc tacacggat tatgtcaaa 
      gatgcgaat aaattgaaa ggtgggatc agtccgtgg 
      ctaccattc cgcgaaccc acctcatgt tccaaagcc 
      tacgacgat ccgagactg attttaaaa tcaagggtc 
      ttccatgcc gttattttg gtcatttta caaaaggcc 
      gttcgagga caaattccc gaagatgta acagcttta 
      attattttc ctattggat caagcggtg accattagc 
      gagcgaaaa gaagaacag gcggagcca aagttctac 
      caccaccaa tcaaaacca ctaaacgat atgaatttc 
      gacaattgg tactcgtct gattgcatt tttgaaaat 
      ttggccacg cccatcgct aaaattgtg ttaacccca 
      gaacctgaa gtctccccc atgacgtat agtagtgat 
      agcgacatc gagtgggaa aattcggaa gttgatacg 
      gaaatgtcc gatgttaat ccggatggg aataatatt 
      ttgatgctg caagattca gccggttgg gaccaatca 
      ggtaccgat ttgatggtg tttgttccg caagactcc 
      tcccccggt tttgagccg accaatccc gtgctagct 
      ctcccaccg tcgacgtca atggacgtg gataatatg 
      actgttgcc gtgacgacg cgttctgtt gtccccgcc 
      cattcggaa ccgcctgca cttccggcg attacggcc 
      ggaaatgaa gttgcgaga ccttggact gggaatgag 
      ttggttccg tcaacgtca agcgcctcg ggtcatagg 
      aggccgtac aggaggcgg ccgactttg gaagggggc 
      ggggcgact ccgcccact gttgaactg aacgaaagt 
      ataatttcg ttgttgtta aagctgcat tcgcaattg 
      tcgggcgtt ccggatagt tttaaaggg gaggaggag 
      gatgatgaa gggcgtggt ttgattggc gacgggccg 
      ttttttatt ggacgtttg ttgaggaaa atctcgcga 
      ttggatgag gagtgtcga aggtgtgtc ttggaggcg 
      aggataaag ttgtggccc agtcaggaa gaacgggac 
      gaagcgaaa cgacaaaga gagaatcgc gagaaggag 
      gaaagaagg aggagagca aaagaaaga caacagaaa 
      ttaatggca gagtttgct tcaaaacaa aaacagttt 
      atggaagcg atggaaacg gatgataac gttttgaat 
      tcggacgag gagttgttt gtgagtaaa caggagtac 
      gattgtgtg atttgtaac caagccacg cccagtact 
      gaggagaaa ccgatgggg ttggttgtt tggattcaa 
      gcgactagt gttatcggg cataggcgg agacatggt 
      cagtcagtg ttgccaact tgtgatgat gaaagaacg 
      tcattgaga cgagatgat acgttgagt gccgagttt 
      gaccgacgg ttggaggaa ttgaatcaa tattttgat 
      cctaaatct tggtactta tcagtgaac gttggatgg 
      gatggaggt gttcatgtg caaacttgt ggccaccat 
      cttcaccta gactgtctt aattcgtat ttgcaaact 
      ttgaggtca caacaacgt ggtgagtat ttttgtccg 
      ctttgtcgg caattggcg aactcggtc ctgcccctg 
      gcgccccaa ttgggcgat tgtgcccaa atggttcgc 
      tcgaatcct tccagtatg tcgcaaatt ttggacgaa 
      ttgagcgat tttttgcgt gaaaacgac caaaaacct 
      agtaataca agtatggcg gaagcgatc ggaaaaata 
      atggaagtt atgacaagt agtacacaa ttgaaaccg 
      atgcttcga tcgaaaagt gatcgtcct atacctcaa 
      agtctcttc ctatttgtc acatcaatc gctcggacc 
      aatctcgag acggaattg agtcaacga ggcgggact 
      ttagtgatg tcacgtgac ccacctaat ccactccta 
      ccaaaacgc gattgtata gttcctcta cttcatgta 
      ctttcggtc cacgctcgc gtggctcga gtgttggct 
      atgtggccg gcttggatg accttccag cagctgtgt 
      ggtttgccc ccgaaaccc ccaagtgat acggcactg 
      gcgccggtc gaaaacgaa gtgccgcta ctgctcagg 
      gatccagtg gcgcttctt ttgcaattt atcctgctt 
      ttgccgcta catctagat caaaattat ttcataacg 
      acagtccaa atggtatac aatttattg tattaccaa 
      gtggtggcg cagatatcc tgcggttta accggagcc 
      gagcgcgcc cgagccacg gcgggcgct aacgaaggc 
      aaaatttgt acgttaacc gatgccctt gcccttatc 
      aatcggtgt ttgggacac actgcgtta tacatggag 
      gacgatgaa gagtgcacc gaaaaacct caagtcaat 
      atggtggct cttgagcta caagttcaa aaattatgt 
      ctgccttat ctacgaatc gctgctctt ctcaaatat 
      cacatctat cagcaagcc ttgcccgat gtgagggca 
      cccgagacc gaattcgtt tgtttggtg cattatttg 
      gaattggtt actgaacag gtcacgtgg gactgtaat 
      gccgccgct gctttgaat tggccaatc gaaacacag 
      atgtataca gcatcacca caatcgtgg tgtaaccag 
      tatgcggtt ttcgttgct aaaagtcag atagcagca 
      cggtcgttc cttgtggat caacacatc acgtggcat 
      ccgccacgc ctacttaaa ttgccgagg gagtacgag 
      aagattttc acctactat cacaagagg ccgtgtagt 
      caatgccag tctgtcccg caagaaacg ggcatttgt 
      ttaatgtgt ggcaccatc gtgtgtttg aaacagtca 
      tgttgcaaa caacagaac atctgtgag gcgactgcg 
      catgcgtcg gagtgtggt gccggcacc ggtgttttt 
      ctcgtagtt acatcgact tatattatt gtaataaga 
      aggcacagg gcgtgtctt tggggttcg ttatacttg 
      gatgatttt gaagaggaa gatcgggat ttgaagcgc 
      gggaaaccg ttgtatttg agtagggag aggtaccaa 
      ctgttggag cagcaatgg ctagcgcat cggtttgac 
      cacatcgaa aaaacgtgg atccagcac ggaaatgct 
      ctttaattg tatatttat gaattgtga taaagataa 
      atgatgtct tttttcgaa tgttatgta tattttcgt 
      ggtgtttac ttttattct agtctattg gtaggttga 
      ctaacacag ggcctaatt ctttttacg tattttttt 
      tttgttaga ttttagagt gtttttctt gttgggaga 
      gttcatcaa gattaattc aaaattatt atgtacata 
      ttagagtta cgatagtat tgttcacac gtaaagtga 
      tttatttta gtgtggaag tactgtaat ggcttgtga 
      taatcacgt aggtttaag ttatgatgt gggtcagtt 
      ctaatacgt ccatatcag tgtcttcaa acacatgtg 
      aatttgttc atacgcgcg ctaattatt tattttgag 
      gttattacg atcaaggag cgccacgaa ataactagc 
      tcgttcatt tctttgaaa tatctgtaa atgataaat 
      aaataacct tgtacataa tatggaaaa gcacagctt 
      tcttgatca gtgaagaat gaaggaagg tgagtaaca 
      ttctttttt tctgttttc cggagtact ttgggttta 
      tcaagtgac caaataaat ttgtaatga atttgggta 
      acagttttc aggttggta aaacttggc aacttgact 
      atcaaattg tcaaaatga cgataactc acttttgcc 
      aatatattt ttttgacac aatctttta tggtcaccc 
      tgtatatat tagctgtat aaactatat aaacgctaa 
      cgtcgcatt ttaccaaat tggataata cgtcaattg 
      aaaactgaa aataaaatt ttatttgtt gttccaata 
      aaaatttat tttttttta atacaatga cagaaccca 
      gaacgagaa taagcacaa attaggtgt ccggtgaat 
      actaatctg aactctact tgtacggaa ttttttcgg 
      atcctaaaa atgaggata tcttaaaaa cagcttatt 
      tttcgaaga aataaattt tggcgagtg atctcttag 
      ttcacagat caggttaag atgcttttt gattttctt 
      tggttgaga aaacaaaga aaagaagac aaaactggc 
      agaagcgat aaacggtct cagaaaaca gtaagaaga 
      tagtaaaag aaggagata ggagggatg acaaaagtg 
      gaattaaag aaagattag tgactggaa gataagaat 
      attgaacaa ggaaagaag atagaacac aaaggttaa 
      aaatgatag aagagaata tagaagatt ttgataaaa 
      aaaaagaaa tatttctat ttaatgtca gatagagaa 
      taacgcgtg aaatagaaa ataaatata ggggaaata 
      aagcataaa agctaaacg ggaaaggag aaacagaaa 
      acagcaaaa atgcaagaa atagactat ttaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY