Status
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Gene information
General information
TC005949 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5015642..5035537
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC005949
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00966

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     head appendages shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax segment number increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment potentially transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005949 RA
      MTYIQGNFC NSMVDADLY LLQIACTRM NYNDFISLI 
IDKFHVTES LSLAERPPQ NPYLKPEHN NRMLESFLT 
FLATLVTVR TNIGLSETE LNRLEMATL LCMSDRTHS 
QLMELMPER CGTSQTRDF ETLLAELAD YKPPVLDPS 
GGNLQQGMY SAKPHVWEN LYDPIHVLL RAVHRRDFH 
TSMDRYTDY VKDANKLKG GISPWLPFR EPTSCSKAY 
DDPRLILKS RVFHAVILV ILQKAVRGQ IPEDVTALI 
IFLLDQAVT ISERKEEQA EPKFYHHQS KPLNDMNFD 
NWYSSDCIF ENLATPIAK IVLTPEPEV SPMTYSSDS 
DIEWENSEV DTEMSDVNP DGNNILMLQ DSAGWDQSG 
TDLMVFVPQ DSSPGFEPT NPVLALPPS TSMDVDNMT 
VAVTTRSVV PAHSEPPAL PAITAGNEV ARPWTGNEL 
VPSTSSASG HRRPYRRRP TLEGGGATP PTVELNESI 
ISLLLKLHS QLSGVPDSF KGEEEDDEG RGLIGDGPF 
FIGRLLRKI SRLDEECRR CVLEARIKL WPSQEERDE 
AKRQRENRE KEERRRRAK ERQQKLMAE FASKQKQFM 
EAMETDDNV LNSDEELFV SKQEYDCVI CNQATPSTE 
EKPMGLVVW IQATSVIGH RRRHGQSVL PTCDDERTS 
LRRDDTLSA EFDRRLEEL NQYFDPKSW YLSVNVGWD 
GGVHVQTCG HHLHLDCLN SYLQTLRSQ QRGEYFCPL 
CRQLANSVL PLAPQLGDC AQMVRSNPS SMSQILDEL 
SDFLRENDQ KPSNTSMAE AIGKIMEVM TSSTQLKPM 
LRSKSDRPI PQSLFLFVT SIARTNLET ELSQRGGTL 
VMSRDPPNP LLPKRDCIV PLLHVLSVH ARVARVLAM 
WPAWMTFQQ LCGLPPKPP SDTALAPVE NEVPLLLRD 
PVALLLQFI LLLPLHLDQ NYFITTVQM VYNLLYYQV 
VAQISCGLT GAERARATA GANEGKICT LTDALALIN 
RCLGHTALY MEDDEECTE KPQVNMVAL ELQVQKLCL 
PYLRIAALL KYHIYQQAL PDVRAPETE FVCLVHYLE 
LVTEQVTWD CNAAAALNW PIETQMYTA SPQSWCNQY 
AVFVAKSQI AARSFLVDQ HITWHPPRL LKLPREYEK 
IFTYYHKRP CSQCQSVPQ ETGICLMCG TIVCLKQSC 
CKQQNICEA TAHASECGA GTGVFLVVT STYIIVIRR 
HRACLWGSL YLDDFEEED RDLKRGKPL YLSRERYQL 
LEQQWLAHR FDHIEKTWI QHGNAL
    CDS sequence
    • >TC005949 RA
      atgacttac atccaagga aatttctgt aattcgatg 
gtggacgcc gatttgtat ctcctccaa atcgcatgt 
acgcgaatg aattacaac gacttcatc tctcttatt 
atcgacaag ttccacgta accgaatct ctgagtctt 
gctgaacga cctcctcaa aacccctac cttaaaccc 
gaacataac aatcgaatg ttggagagt ttcttgacg 
tttttggcc actcttgta acagttcgg acgaatatt 
ggattgtcc gaaaccgaa ctgaatcgt ctcgaaatg 
gcaacgctg ttgtgtatg agtgatcgg acgcattcg 
caactaatg gaattgatg ccggaacgt tgtggtacg 
tctcaaacg cgcgatttt gagacactt ttggccgaa 
ctggccgat tataaaccg ccagttttg gatcccagt 
ggtggtaat ttacagcaa gggatgtat tcggctaag 
ccacacgtt tgggagaat ttatacgat ccaattcat 
gtgctgtta cgtgccgta catcgacgt gatttccac 
acgtcgatg gatcgctac acggattat gtcaaagat 
gcgaataaa ttgaaaggt gggatcagt ccgtggcta 
ccattccgc gaacccacc tcatgttcc aaagcctac 
gacgatccg agactgatt ttaaaatca agggtcttc 
catgccgtt attttggtc attttacaa aaggccgtt 
cgaggacaa attcccgaa gatgtaaca gctttaatt 
attttccta ttggatcaa gcggtgacc attagcgag 
cgaaaagaa gaacaggcg gagccaaag ttctaccac 
caccaatca aaaccacta aacgatatg aatttcgac 
aattggtac tcgtctgat tgcattttt gaaaatttg 
gccacgccc atcgctaaa attgtgtta accccagaa 
cctgaagtc tcccccatg acgtatagt agtgatagc 
gacatcgag tgggaaaat tcggaagtt gatacggaa 
atgtccgat gttaatccg gatgggaat aatattttg 
atgctgcaa gattcagcc ggttgggac caatcaggt 
accgatttg atggtgttt gttccgcaa gactcctcc 
cccggtttt gagccgacc aatcccgtg ctagctctc 
ccaccgtcg acgtcaatg gacgtggat aatatgact 
gttgccgtg acgacgcgt tctgttgtc cccgcccat 
tcggaaccg cctgcactt ccggcgatt acggccgga 
aatgaagtt gcgagacct tggactggg aatgagttg 
gttccgtca acgtcaagc gcctcgggt cataggagg 
ccgtacagg aggcggccg actttggaa gggggcggg 
gcgactccg cccactgtt gaactgaac gaaagtata 
atttcgttg ttgttaaag ctgcattcg caattgtcg 
ggcgttccg gatagtttt aaaggggag gaggaggat 
gatgaaggg cgtggtttg attggcgac gggccgttt 
tttattgga cgtttgttg aggaaaatc tcgcgattg 
gatgaggag tgtcgaagg tgtgtcttg gaggcgagg 
ataaagttg tggcccagt caggaagaa cgggacgaa 
gcgaaacga caaagagag aatcgcgag aaggaggaa 
agaaggagg agagcaaaa gaaagacaa cagaaatta 
atggcagag tttgcttca aaacaaaaa cagtttatg 
gaagcgatg gaaacggat gataacgtt ttgaattcg 
gacgaggag ttgtttgtg agtaaacag gagtacgat 
tgtgtgatt tgtaaccaa gccacgccc agtactgag 
gagaaaccg atggggttg gttgtttgg attcaagcg 
actagtgtt atcgggcat aggcggaga catggtcag 
tcagtgttg ccaacttgt gatgatgaa agaacgtca 
ttgagacga gatgatacg ttgagtgcc gagtttgac 
cgacggttg gaggaattg aatcaatat tttgatcct 
aaatcttgg tacttatca gtgaacgtt ggatgggat 
ggaggtgtt catgtgcaa acttgtggc caccatctt 
cacctagac tgtcttaat tcgtatttg caaactttg 
aggtcacaa caacgtggt gagtatttt tgtccgctt 
tgtcggcaa ttggcgaac tcggtcctg cccctggcg 
ccccaattg ggcgattgt gcccaaatg gttcgctcg 
aatccttcc agtatgtcg caaattttg gacgaattg 
agcgatttt ttgcgtgaa aacgaccaa aaacctagt 
aatacaagt atggcggaa gcgatcgga aaaataatg 
gaagttatg acaagtagt acacaattg aaaccgatg 
cttcgatcg aaaagtgat cgtcctata cctcaaagt 
ctcttccta tttgtcaca tcaatcgct cggaccaat 
ctcgagacg gaattgagt caacgaggc gggacttta 
gtgatgtca cgtgaccca cctaatcca ctcctacca 
aaacgcgat tgtatagtt cctctactt catgtactt 
tcggtccac gctcgcgtg gctcgagtg ttggctatg 
tggccggct tggatgacc ttccagcag ctgtgtggt 
ttgcccccg aaaccccca agtgatacg gcactggcg 
ccggtcgaa aacgaagtg ccgctactg ctcagggat 
ccagtggcg cttcttttg caatttatc ctgcttttg 
ccgctacat ctagatcaa aattatttc ataacgaca 
gtccaaatg gtatacaat ttattgtat taccaagtg 
gtggcgcag atatcctgc ggtttaacc ggagccgag 
cgcgcccga gccacggcg ggcgctaac gaaggcaaa 
atttgtacg ttaaccgat gcccttgcc cttatcaat 
cggtgtttg ggacacact gcgttatac atggaggac 
gatgaagag tgcaccgaa aaacctcaa gtcaatatg 
gtggctctt gagctacaa gttcaaaaa ttatgtctg 
ccttatcta cgaatcgct gctcttctc aaatatcac 
atctatcag caagccttg cccgatgtg agggcaccc 
gagaccgaa ttcgtttgt ttggtgcat tatttggaa 
ttggttact gaacaggtc acgtgggac tgtaatgcc 
gccgctgct ttgaattgg ccaatcgaa acacagatg 
tatacagca tcaccacaa tcgtggtgt aaccagtat 
gcggttttc gttgctaaa agtcagata gcagcacgg 
tcgttcctt gtggatcaa cacatcacg tggcatccg 
ccacgccta cttaaattg ccgagggag tacgagaag 
attttcacc tactatcac aagaggccg tgtagtcaa 
tgccagtct gtcccgcaa gaaacgggc atttgttta 
atgtgtggc accatcgtg tgtttgaaa cagtcatgt 
tgcaaacaa cagaacatc tgtgaggcg actgcgcat 
gcgtcggag tgtggtgcc ggcaccggt gtttttctc 
gtagttaca tcgacttat attattgta ataagaagg 
cacagggcg tgtctttgg ggttcgtta tacttggat 
gattttgaa gaggaagat cgggatttg aagcgcggg 
aaaccgttg tatttgagt agggagagg taccaactg 
ttggagcag caatggcta gcgcatcgg tttgaccac 
atcgaaaaa acgtggatc cagcacgga aatgctctt 
taa
    mRNA sequence
    • >TC005949 RA
      caagaacga aatcttttt gagtaaaaa ttcttattt 
ttttttcag gtggcgttt tacgaaatt ctgaacgac 
ttgtgggtc cgcaacggt ctccaaatc aagggccaa 
gccatgact tacatccaa ggaaatttc tgtaattcg 
atggtggac gccgatttg tatctcctc caaatcgca 
tgtacgcga atgaattac aacgacttc atctctctt 
attatcgac aagttccac gtaaccgaa tctctgagt 
cttgctgaa cgacctcct caaaacccc taccttaaa 
cccgaacat aacaatcga atgttggag agtttcttg 
acgtttttg gccactctt gtaacagtt cggacgaat 
attggattg tccgaaacc gaactgaat cgtctcgaa 
atggcaacg ctgttgtgt atgagtgat cggacgcat 
tcgcaacta atggaattg atgccggaa cgttgtggt 
acgtctcaa acgcgcgat tttgagaca cttttggcc 
gaactggcc gattataaa ccgccagtt ttggatccc 
agtggtggt aatttacag caagggatg tattcggct 
aagccacac gtttgggag aatttatac gatccaatt 
catgtgctg ttacgtgcc gtacatcga cgtgatttc 
cacacgtcg atggatcgc tacacggat tatgtcaaa 
gatgcgaat aaattgaaa ggtgggatc agtccgtgg 
ctaccattc cgcgaaccc acctcatgt tccaaagcc 
tacgacgat ccgagactg attttaaaa tcaagggtc 
ttccatgcc gttattttg gtcatttta caaaaggcc 
gttcgagga caaattccc gaagatgta acagcttta 
attattttc ctattggat caagcggtg accattagc 
gagcgaaaa gaagaacag gcggagcca aagttctac 
caccaccaa tcaaaacca ctaaacgat atgaatttc 
gacaattgg tactcgtct gattgcatt tttgaaaat 
ttggccacg cccatcgct aaaattgtg ttaacccca 
gaacctgaa gtctccccc atgacgtat agtagtgat 
agcgacatc gagtgggaa aattcggaa gttgatacg 
gaaatgtcc gatgttaat ccggatggg aataatatt 
ttgatgctg caagattca gccggttgg gaccaatca 
ggtaccgat ttgatggtg tttgttccg caagactcc 
tcccccggt tttgagccg accaatccc gtgctagct 
ctcccaccg tcgacgtca atggacgtg gataatatg 
actgttgcc gtgacgacg cgttctgtt gtccccgcc 
cattcggaa ccgcctgca cttccggcg attacggcc 
ggaaatgaa gttgcgaga ccttggact gggaatgag 
ttggttccg tcaacgtca agcgcctcg ggtcatagg 
aggccgtac aggaggcgg ccgactttg gaagggggc 
ggggcgact ccgcccact gttgaactg aacgaaagt 
ataatttcg ttgttgtta aagctgcat tcgcaattg 
tcgggcgtt ccggatagt tttaaaggg gaggaggag 
gatgatgaa gggcgtggt ttgattggc gacgggccg 
ttttttatt ggacgtttg ttgaggaaa atctcgcga 
ttggatgag gagtgtcga aggtgtgtc ttggaggcg 
aggataaag ttgtggccc agtcaggaa gaacgggac 
gaagcgaaa cgacaaaga gagaatcgc gagaaggag 
gaaagaagg aggagagca aaagaaaga caacagaaa 
ttaatggca gagtttgct tcaaaacaa aaacagttt 
atggaagcg atggaaacg gatgataac gttttgaat 
tcggacgag gagttgttt gtgagtaaa caggagtac 
gattgtgtg atttgtaac caagccacg cccagtact 
gaggagaaa ccgatgggg ttggttgtt tggattcaa 
gcgactagt gttatcggg cataggcgg agacatggt 
cagtcagtg ttgccaact tgtgatgat gaaagaacg 
tcattgaga cgagatgat acgttgagt gccgagttt 
gaccgacgg ttggaggaa ttgaatcaa tattttgat 
cctaaatct tggtactta tcagtgaac gttggatgg 
gatggaggt gttcatgtg caaacttgt ggccaccat 
cttcaccta gactgtctt aattcgtat ttgcaaact 
ttgaggtca caacaacgt ggtgagtat ttttgtccg 
ctttgtcgg caattggcg aactcggtc ctgcccctg 
gcgccccaa ttgggcgat tgtgcccaa atggttcgc 
tcgaatcct tccagtatg tcgcaaatt ttggacgaa 
ttgagcgat tttttgcgt gaaaacgac caaaaacct 
agtaataca agtatggcg gaagcgatc ggaaaaata 
atggaagtt atgacaagt agtacacaa ttgaaaccg 
atgcttcga tcgaaaagt gatcgtcct atacctcaa 
agtctcttc ctatttgtc acatcaatc gctcggacc 
aatctcgag acggaattg agtcaacga ggcgggact 
ttagtgatg tcacgtgac ccacctaat ccactccta 
ccaaaacgc gattgtata gttcctcta cttcatgta 
ctttcggtc cacgctcgc gtggctcga gtgttggct 
atgtggccg gcttggatg accttccag cagctgtgt 
ggtttgccc ccgaaaccc ccaagtgat acggcactg 
gcgccggtc gaaaacgaa gtgccgcta ctgctcagg 
gatccagtg gcgcttctt ttgcaattt atcctgctt 
ttgccgcta catctagat caaaattat ttcataacg 
acagtccaa atggtatac aatttattg tattaccaa 
gtggtggcg cagatatcc tgcggttta accggagcc 
gagcgcgcc cgagccacg gcgggcgct aacgaaggc 
aaaatttgt acgttaacc gatgccctt gcccttatc 
aatcggtgt ttgggacac actgcgtta tacatggag 
gacgatgaa gagtgcacc gaaaaacct caagtcaat 
atggtggct cttgagcta caagttcaa aaattatgt 
ctgccttat ctacgaatc gctgctctt ctcaaatat 
cacatctat cagcaagcc ttgcccgat gtgagggca 
cccgagacc gaattcgtt tgtttggtg cattatttg 
gaattggtt actgaacag gtcacgtgg gactgtaat 
gccgccgct gctttgaat tggccaatc gaaacacag 
atgtataca gcatcacca caatcgtgg tgtaaccag 
tatgcggtt ttcgttgct aaaagtcag atagcagca 
cggtcgttc cttgtggat caacacatc acgtggcat 
ccgccacgc ctacttaaa ttgccgagg gagtacgag 
aagattttc acctactat cacaagagg ccgtgtagt 
caatgccag tctgtcccg caagaaacg ggcatttgt 
ttaatgtgt ggcaccatc gtgtgtttg aaacagtca 
tgttgcaaa caacagaac atctgtgag gcgactgcg 
catgcgtcg gagtgtggt gccggcacc ggtgttttt 
ctcgtagtt acatcgact tatattatt gtaataaga 
aggcacagg gcgtgtctt tggggttcg ttatacttg 
gatgatttt gaagaggaa gatcgggat ttgaagcgc 
gggaaaccg ttgtatttg agtagggag aggtaccaa 
ctgttggag cagcaatgg ctagcgcat cggtttgac 
cacatcgaa aaaacgtgg atccagcac ggaaatgct 
ctttaattg tatatttat gaattgtga taaagataa 
atgatgtct tttttcgaa tgttatgta tattttcgt 
ggtgtttac ttttattct agtctattg gtaggttga 
ctaacacag ggcctaatt ctttttacg tattttttt 
tttgttaga ttttagagt gtttttctt gttgggaga 
gttcatcaa gattaattc aaaattatt atgtacata 
ttagagtta cgatagtat tgttcacac gtaaagtga 
tttatttta gtgtggaag tactgtaat ggcttgtga 
taatcacgt aggtttaag ttatgatgt gggtcagtt 
ctaatacgt ccatatcag tgtcttcaa acacatgtg 
aatttgttc atacgcgcg ctaattatt tattttgag 
gttattacg atcaaggag cgccacgaa ataactagc 
tcgttcatt tctttgaaa tatctgtaa atgataaat 
aaataacct tgtacataa tatggaaaa gcacagctt 
tcttgatca gtgaagaat gaaggaagg tgagtaaca 
ttctttttt tctgttttc cggagtact ttgggttta 
tcaagtgac caaataaat ttgtaatga atttgggta 
acagttttc aggttggta aaacttggc aacttgact 
atcaaattg tcaaaatga cgataactc acttttgcc 
aatatattt ttttgacac aatctttta tggtcaccc 
tgtatatat tagctgtat aaactatat aaacgctaa 
cgtcgcatt ttaccaaat tggataata cgtcaattg 
aaaactgaa aataaaatt ttatttgtt gttccaata 
aaaatttat tttttttta atacaatga cagaaccca 
gaacgagaa taagcacaa attaggtgt ccggtgaat 
actaatctg aactctact tgtacggaa ttttttcgg 
atcctaaaa atgaggata tcttaaaaa cagcttatt 
tttcgaaga aataaattt tggcgagtg atctcttag 
ttcacagat caggttaag atgcttttt gattttctt 
tggttgaga aaacaaaga aaagaagac aaaactggc 
agaagcgat aaacggtct cagaaaaca gtaagaaga 
tagtaaaag aaggagata ggagggatg acaaaagtg 
gaattaaag aaagattag tgactggaa gataagaat 
attgaacaa ggaaagaag atagaacac aaaggttaa 
aaatgatag aagagaata tagaagatt ttgataaaa 
aaaaagaaa tatttctat ttaatgtca gatagagaa 
taacgcgtg aaatagaaa ataaatata ggggaaata 
aagcataaa agctaaacg ggaaaggag aaacagaaa 
acagcaaaa atgcaagaa atagactat ttaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY