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Gene information
General information
TC005946 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5078871..5093501
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005946
    iBeetle screen iB_00965

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005946 RA
      MDNGVKVRM SGYLHLKTK KRMISSWKN YWFVLEDRL 
      LLYYRSKAE YETISPCKG SINLGPPCM VKSLPSVTN 
      SFQVETRSL TYTLKADNS DDQKKWMQA ILAVLNQNQ 
      NFPSESPKR LSHFRYSLD DLFPQTEPN DPQTPPKAP 
      LERQNTLPH RLKLEPKKS NEIIQRLQK LGAQSYGGS 
      LGAISKIAT KKVTKEEKC KNVSQGSLP SCLFNEEDE 
      FEEVVRPTT NNREENIYE RISGDSQYS GESVKYSVP 
      LRRGFSVSE QRDKGATSR ETKSLLVEN DQYATTRHV 
      QSDEVIYAE PKLDCNKNS LLVENTFYA SCDVVGHTN 
      DNIYEDLDK YNPYSEPVL DSSQSSGKK EKIKKSKSF 
      IRRVWKKKY RKQDEKKVE EEIYAEVDP TVVNKVDMS 
      DDSAVQMLS ELQNILENK KAQLRAKAS NNPTAETAE 
      STAKPDEEE EEEEAPKLP PKIHPPEQF RSLDEILED 
      LDREKLESK GKVQLLIEK FTESEPDVI LRKHDKSGY 
      RYSDDLNLL LDELAKVTS APILTPGVT TSLVNPNLT 
      DEEWLKLLP VRQRRLSEP DYDIPRPLK PVQTPEKSE 
      NTDVIPATR FFGPVLPPT IIDNKDESL CVSMTPDSL 
      EIDSQKFQT LCSYTSHNY TDLKCFQKK DDVIYALPK 
      SYLSTHIVN TCHNLCGGN LDECQIIYK NVKNIEHEE 
      IFNDSLELC TETKTEF
    CDS sequence
    • >TC005946 RA
      atggacaat ggagtcaaa gtgcggatg tccggatat 
      ctccacttg aagaccaaa aagcgcatg atatcatca 
      tggaaaaat tactggttt gtgttggaa gatcggtta 
      ttgctgtat tacaggtcg aaggccgaa tatgagaca 
      atttcgccc tgcaagggc tccattaat ctggggccc 
      ccttgcatg gtcaaatct ctgccttcg gtaacgaac 
      tcctttcaa gtggagaca cgaagtttg acctacact 
      ttgaaagcg gacaactcc gacgaccag aaaaaatgg 
      atgcaagcc atcctggcc gtccttaac cagaaccaa 
      aacttcccc agcgaaagt cccaaacga ctgagccac 
      ttccgctac tccctcgac gacttattc cctcaaact 
      gaacccaac gacccccag acaccgcca aaagcgccc 
      ctcgaacgc caaaacaca ctcccccat cgactaaaa 
      ttagaaccc aaaaaaagt aatgaaata atccagagg 
      ttacagaaa ctaggggcg caaagctat ggcggtagt 
      ttgggcgca atcagtaaa atagcgacg aaaaaagtc 
      accaaggag gagaaatgc aaaaatgtg tctcagggc 
      tcgttacca agctgcctt ttcaacgaa gaggacgag 
      ttcgaggaa gttgttaga ccaacaacc aacaacagg 
      gaggaaaat atttacgag cggatttcg ggcgattcg 
      caatattcg ggggaaagt gtaaagtac agtgttccg 
      ttgaggcgg ggcttcagt gtgagtgag caaagggac 
      aagggggcc acatctcgc gaaacgaag agtttattg 
      gtggaaaat gaccaatac gcaaccact agacatgtc 
      caaagtgac gaagttatt tacgctgag cccaagttg 
      gactgtaat aaaaatagt cttttggtt gagaacacg 
      ttttatgct agttgcgat gttgtgggg cacacgaat 
      gataatatt tacgaggat ttggataag tataaccct 
      tacagtgag ccagttttg gattctagt caaagttcc 
      ggcaaaaaa gagaaaatc aaaaaatcg aaaagtttc 
      attcggcga gtttggaag aagaagtat cgaaaacaa 
      gacgagaaa aaggttgag gaggaaatt tacgcagag 
      gttgacccg actgtagtt aacaaagtc gatatgagc 
      gatgacagc gctgttcaa atgttgtca gagcttcaa 
      aacatttta gaaaataag aaagcacag ttgagggcc 
      aaggcctcc aataaccca acggccgaa accgcggaa 
      tcaacagca aaaccagac gaagaagaa gaagaagaa 
      gaagccccg aaattgccc cccaaaata caccctccc 
      gaacagttc cgaagtctc gacgaaatc cttgaagat 
      ttagaccgg gaaaagctc gaatccaag ggcaaagtg 
      cagctccta attgaaaaa tttacggag tcggaaccg 
      gacgtaatt ttgcgaaaa catgacaaa tctgggtat 
      cgatattcg gatgatctt aatttatta ctggatgaa 
      cttgcgaaa gtcacttcg gcccccatt ttgacgcct 
      ggcgtgacc acaagtctc gtcaacccc aatcttacc 
      gatgaagag tggctgaaa ctcctcccc gtgcggcag 
      agaaggtta tcagaaccg gactacgat attccgcgc 
      ccccttaag ccggtccag accccggaa aagtcggaa 
      aataccgat gtcatcccc gcgactcgg tttttcgga 
      ccagtgcta ccacccaca atcattgac aacaaagac 
      gagtctttg tgtgttagt atgactcca gattcgctg 
      gaaatcgat tcgcaaaaa ttccagact ttgtgttcc 
      tacacgtcc cataattat accgatttg aaatgtttt 
      caaaaaaaa gacgacgtt atttacgca ctgcccaag 
      tcatacctc tccacgcat attgtaaac acgtgtcac 
      aacttgtgc ggtggaaat ttggacgag tgtcagatt 
      atttacaaa aatgtgaag aatatcgaa catgaagaa 
      atcttcaac gattccctc gaactttgt accgaaact 
      aagacagag ttctaa
    mRNA sequence
    • >TC005946 RA
      atacgctac aattccaca acataaacg cgcaactat 
      cgcaacgga agtttactt ccgcaaggg tccgactgg 
      caaaagacc acgacgtag ccttgtgct gactgtgcg 
      ctccgtttg aatttccga gtaacagtt ttgaaaaga 
      tgcgccttt ttcaaatcg tgctgcgaa tgtgagcgg 
      cgagagtgt cacagttca ggccaatca tggacaatg 
      gagtcaaag tgcggatgt ccggatatc tccacttga 
      agaccaaaa agcgcatga tatcatcat ggaaaaatt 
      actggtttg tgttggaag atcggttat tgctgtatt 
      acaggtcga aggccgaat atgagacaa tttcgccct 
      gcaagggct ccattaatc tggggcccc cttgcatgg 
      tcaaatctc tgccttcgg taacgaact cctttcaag 
      tggagacac gaagtttga cctacactt tgaaagcgg 
      acaactccg acgaccaga aaaaatgga tgcaagcca 
      tcctggccg tccttaacc agaaccaaa acttcccca 
      gcgaaagtc ccaaacgac tgagccact tccgctact 
      ccctcgacg acttattcc ctcaaactg aacccaacg 
      acccccaga caccgccaa aagcgcccc tcgaacgcc 
      aaaacacac tcccccatc gactaaaat tagaaccca 
      aaaaaagta atgaaataa tccagaggt tacagaaac 
      taggggcgc aaagctatg gcggtagtt tgggcgcaa 
      tcagtaaaa tagcgacga aaaaagtca ccaaggagg 
      agaaatgca aaaatgtgt ctcagggct cgttaccaa 
      gctgccttt tcaacgaag aggacgagt tcgaggaag 
      ttgttagac caacaacca acaacaggg aggaaaata 
      tttacgagc ggatttcgg gcgattcgc aatattcgg 
      gggaaagtg taaagtaca gtgttccgt tgaggcggg 
      gcttcagtg tgagtgagc aaagggaca agggggcca 
      catctcgcg aaacgaaga gtttattgg tggaaaatg 
      accaatacg caaccacta gacatgtcc aaagtgacg 
      aagttattt acgctgagc ccaagttgg actgtaata 
      aaaatagtc ttttggttg agaacacgt tttatgcta 
      gttgcgatg ttgtggggc acacgaatg ataatattt 
      acgaggatt tggataagt ataaccctt acagtgagc 
      cagttttgg attctagtc aaagttccg gcaaaaaag 
      agaaaatca aaaaatcga aaagtttca ttcggcgag 
      tttggaaga agaagtatc gaaaacaag acgagaaaa 
      aggttgagg aggaaattt acgcagagg ttgacccga 
      ctgtagtta acaaagtcg atatgagcg atgacagcg 
      ctgttcaaa tgttgtcag agcttcaaa acattttag 
      aaaataaga aagcacagt tgagggcca aggcctcca 
      ataacccaa cggccgaaa ccgcggaat caacagcaa 
      aaccagacg aagaagaag aagaagaag aagccccga 
      aattgcccc ccaaaatac accctcccg aacagttcc 
      gaagtctcg acgaaatcc ttgaagatt tagaccggg 
      aaaagctcg aatccaagg gcaaagtgc agctcctaa 
      ttgaaaaat ttacggagt cggaaccgg acgtaattt 
      tgcgaaaac atgacaaat ctgggtatc gatattcgg 
      atgatctta atttattac tggatgaac ttgcgaaag 
      tcacttcgg cccccattt tgacgcctg gcgtgacca 
      caagtctcg tcaacccca atcttaccg atgaagagt 
      ggctgaaac tcctccccg tgcggcaga gaaggttat 
      cagaaccgg actacgata ttccgcgcc cccttaagc 
      cggtccaga ccccggaaa agtcggaaa ataccgatg 
      tcatccccg cgactcggt ttttcggac cagtgctac 
      cacccacaa tcattgaca acaaagacg agtctttgt 
      gtgttagta tgactccag attcgctgg aaatcgatt 
      cgcaaaaat tccagactt tgtgttcct acacgtccc 
      ataattata ccgatttga aatgttttc aaaaaaaag 
      acgacgtta tttacgcac tgcccaagt catacctct 
      ccacgcata ttgtaaaca cgtgtcaca acttgtgcg 
      gtggaaatt tggacgagt gtcagatta tttacaaaa 
      atgtgaaga atatcgaac atgaagaaa tcttcaacg 
      attccctcg aactttgta ccgaaacta agacagagt 
      tctaatctg ttttttact catttatgt at
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