Status
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Gene information
General information
TC034001
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005924
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5500071..5507686
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034001
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_00961

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    pupal molt delayed (still larva) - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    eclosion delayed (still pupa) - Number of animals showing the phenotype: 2 (16 dpi)
    Days post injection



    delayed (still pupa) - Number of animals showing the phenotype: 2 (19 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (22 dpi)
    Days post injection


    Pupal morphology
    antenna orientation bent - Number of animals showing the phenotype: 2

    wings shortened - Number of animals showing the phenotype: 2

    elytra pattern irregular - Number of animals showing the phenotype: 2

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034001 RA
      MAPEKASQV ILETINLDA EQYRLGKTK VFFRAGVLG 
      QMEELRDER LGKIVTWMQ SWVRGYLSR KEFKRLQEQ 
      RLALQVCQR NLRKYLKLR TWPWYKLWQ KVKPLLNVT 
      RIEDEIAKL EEKAAKAQE AYEREAKAK KELEGLYSK 
      LLAEKTELL SSLEGEKGS LSEVQERAN KLQAQKSDL 
      ESQLSETQD RLSQEEDAR NQLMQQKKK LEQEISGYK 
      KDIEDLELN LQKSEQDKA TKDHQIRNL NDEIAHQDE 
      LINKLNKEK KLSGENSQR VSEELQAAE DKVNHLNKV 
      KAKLEQTLD ELEDSLERE KKLRGDVEK SKRKVEGDL 
      KLTQEAVAD LERNKKELE QTIQRKDKE ISSLTAKLE 
      DEQSVVGKL QKQIKELQA RIEELEEEV EAERQARAK 
      AEKQRADLA RELEELGER LEEAGGATS AQIELNKKR 
      EAELAKLRR DLEESNIQH ESTLANLRK KHNDAVSEM 
      GEQIDQLNK LKAKAEKEK AAYFGELND LRASVDHLA 
      NEKAAVEKV SKQLQQQLN DVQGKLDET NRTLNDFDA 
      AKKKLSIEN SDLLRQLEE AESQVSQLS KIKVSLTTQ 
      LEDTKRLAD EEGRERATL LGKFRNLEH DLDNIREQV 
      EEEAEAKAD IQRQLSKAN AEAQLWRQK YESEGVAKS 
      EELEEAKRK LQARLAEAE ETIESLNQK VVALEKTKQ 
      RLATEVEDL QIEVDRANA IANAAEKKQ KAFDKIIGE 
      WKLKVDDLA AELDASQKE CRNYSTELF RLKGAYEEG 
      QEQLEAVRR ENKNLADEV KDLLDQIGE GGRNIHEIE 
      KARKRLEAE KDELQAALE EAEAALEQE ENKVLRSQL 
      ELSQVRQEI DRRIQEKEE EFENTRKNH QRALDSMQA 
      SLEAEAKGK AEALRMKKK LEADINELE IALDHANKA 
      NAEAQKTIK RYQQQLKDT QTALEEEQR ARDEAREQL 
      GISERRANA LQNELEESR TLLEQADRA RRQAEQELG 
      DAHEQLNDL SAQNSSLSA AKRKLETEL QTLHSDLDE 
      LLNEAKNSE EKAKKAMVD AARLADELR AEQDHAQTQ 
      EKLRKALET QIKDLQVRL DEAEANALK GGKKLIQKL 
      EQRVRELEN ELDGEQRRH ADAQKNLRK SERRIKELS 
      FQAEEDRKN HERMQDLVD KLQQKIKTY KRQIEEAEE 
      IAALNLAKF RKAQQELEE AEERADLAE QAIAKFRAK 
      GRGGSVARG GSPAPPRQR PQMDGLTFP PRFDLAPDE 
      I
    CDS sequence
    • >TC034001 RA
      atggcacca gaaaaagca tcgcaagtt attctggaa 
      acgatcaac cttgatgct gaacagtac cgacttggt 
      aaaactaag gtgttcttc cgcgctggt gtgttgggt 
      cagatggaa gagttgcgt gatgagcgt cttggcaag 
      atcgtcacc tggatgcaa tcttgggtg cgtggttac 
      ttgtccagg aaggagttc aagaggttg caggagcaa 
      cgtttggcc ctccaagtg tgccagagg aacctccgc 
      aaatacctc aaactccgc acttggcct tggtacaag 
      ttgtggcag aaagtcaag cctctcctc aacgtcact 
      cgcatcgag gatgagatt gcgaaattg gaggaaaaa 
      gcagccaaa gcacaagaa gcctatgaa cgggaagcc 
      aaagccaag aaggaactg gaaggactt tactccaaa 
      cttttggcc gagaagacc gaactcttg tccagtctt 
      gagggagag aagggatcc ctttccgaa gtacaagaa 
      agggccaac aaactccaa gcccagaag agcgatctt 
      gagtcacaa ttatctgaa actcaagac cgcctcagc 
      caggaggag gatgctcgc aaccagttg atgcaacag 
      aagaagaag cttgagcaa gaaatctcc ggttacaag 
      aaagacatc gaagatctc gaactcaac ctccagaaa 
      tccgagcaa gacaaggcc accaaagac caccagatc 
      cgcaacttg aacgacgaa atcgcccac caagatgag 
      ctcatcaac aagctcaac aaagaaaag aaactttcg 
      ggcgagaac agccaaagg gtgtctgag gaactccaa 
      gccgccgaa gacaaagtc aaccacttg aacaaagtt 
      aaggccaaa ctcgaacaa accctcgac gagctcgaa 
      gactccctc gaacgcgag aagaaactc cgcggggac 
      gtcgaaaag tccaaacgc aaggttgag ggcgacctc 
      aaattgacc caggaagcc gtcgccgac ctcgaacgc 
      aacaagaag gaactcgaa cagaccatc caacgcaaa 
      gacaaggaa atctcctcc ttgaccgcc aaacttgaa 
      gacgagcaa tccgtcgtc ggcaagctc cagaaacag 
      atcaaggag ctccaagcc aggattgag gaattggaa 
      gaggaagtc gaagccgaa cgccaagcc cgcgccaag 
      gccgagaaa cagagagcc gatttggcc agggaattg 
      gaagagttg ggcgaacgt ttggaggaa gctggcggt 
      gccacttcc gcccagatt gaactcaac aagaagcgc 
      gaagccgaa cttgcgaaa ttgaggagg gatttggaa 
      gaatcaaac atccaacat gagtcgact ttggccaac 
      ttgaggaag aagcacaac gatgccgtg tctgaaatg 
      ggtgaacaa atcgaccag ttgaacaaa ctcaaggca 
      aaggctgaa aaagaaaag gctgcatac tttggagaa 
      ctgaacgac cttcgtgcc tccgtcgac catttggcc 
      aacgaaaag gccgctgtt gagaaagtc tccaaacaa 
      ctccaacaa caactcaac gatgtccaa ggcaaactc 
      gacgagacc aaccgcact cttaatgac ttcgacgcc 
      gccaagaag aagctttca atcgaaaac tccgatctt 
      ttgcgccaa ttggaagaa gccgagagc caggtgtcc 
      caattgagc aaaatcaaa gtctcactc accacccaa 
      ttggaagat accaagaga ctcgccgat gaagaaggt 
      cgcgaaagg gccactctt ttgggcaaa ttccgcaat 
      ttggagcac gatttggac aacatccgc gaacaggtt 
      gaagaagaa gctgaagct aaagccgac attcaacgc 
      cagttgagc aaagccaat gccgaagcc caattgtgg 
      agacagaaa tacgaatcc gaaggtgtc gccaagtct 
      gaggaactt gaagaggcc aagaggaaa ctccaagcc 
      aggcttgct gaagccgag gaaaccatc gaatccctc 
      aaccagaag gttgtagct ttggaaaag accaaacag 
      aggctcgcc accgaagtc gaagatttg caaatcgaa 
      gtcgaccgt gctaacgcc atcgccaac gctgctgag 
      aagaaacag aaagcgttc gacaagatc atcggcgaa 
      tggaaactc aaagtcgat gatttggcc gctgaactc 
      gacgccagc cagaaggaa tgccgcaac tactccacc 
      gagctcttc cgtctcaag ggagcctat gaagaaggc 
      caagaacag cttgaagcc gtccgtcgc gaaaacaag 
      aacttggcc gatgaagtt aaggacctc cttgaccaa 
      atcggtgaa ggcggccgc aacatccac gagatcgaa 
      aaggcccgc aaacggctc gaagccgaa aaagacgaa 
      ctccaagcc gctttggaa gaagccgaa gccgctttg 
      gaacaagaa gagaacaaa gttttgcgt tcgcaactc 
      gagttgtct caagttcgt caagaaatc gaccgcagg 
      atccaagag aaggaagaa gaattcgaa aacacccgc 
      aagaaccac caacgcgcc ctcgactcc atgcaagcc 
      tccctcgag gccgaagcc aaaggcaag gctgaggcg 
      ctccgcatg aagaagaag ttggaagcc gacatcaac 
      gagctcgag attgctctc gaccacgcc aacaaggct 
      aacgccgag gcccagaag accatcaaa cgctaccaa 
      cagcaactc aaggacacc caaaccgcc cttgaggag 
      gagcaacgt gctcgtgac gaagcccgc gaacagctc 
      ggaatctcc gaacgccga gccaacgct ctccagaac 
      gagctcgaa gagagcagg actctcctc gagcaagcc 
      gaccgtgct cgccgccaa gccgagcaa gaactgggc 
      gatgctcac gagcaactc aacgacttg tcggcccaa 
      aactcctcc ctttccgcc gccaagagg aaactcgag 
      accgagctc cagactctt cactccgat cttgacgag 
      cttttgaac gaggctaag aactctgaa gagaaggcc 
      aagaaggcc atggtcgac gctgctcgc cttgctgac 
      gagctccgc gccgagcaa gaccacgcc caaacccag 
      gagaagctc cgcaaggcc cttgagacc caaatcaag 
      gacttgcaa gtccgtttg gacgaagcc gaagccaat 
      gctctcaaa ggcggcaag aaactcatc cagaagttg 
      gaacaacgc gtccgcgaa ttggagaac gagctcgat 
      ggcgagcaa cgaagacac gccgatgct cagaagaac 
      ttgaggaag tccgaacgc cgcattaag gaattgagc 
      ttccaagcg gaagaagac cgcaagaac cacgaacgc 
      atgcaggat ttggttgac aaattgcag cagaagatc 
      aagacctac aagaggcag attgaggaa gctgaggaa 
      atcgcagct ctcaatttg gccaagttc aggaaggct 
      caacaggag ttggaagaa gccgaagaa cgtgctgat 
      cttgccgaa caagccatt gctaaattc cgcgcgaag 
      ggacgtgga ggatctgta gccagagga ggcagccca 
      gcgcccccg agacagcgc ccccagatg gacggcctc 
      accttcccc ccaaggttc gacctggct cctgacgag 
      atctaa
    mRNA sequence
    • >TC034001 RA
      ggtcgtggt aagaaggga ggtggtttt gcgacagtg 
      tcgtcagcc tataaggaa caattgaac aaccttatg 
      accactctg cgttcgacc caaccccac ttcgtccgt 
      tgcatcatc cccaatgag ttgaaacag cccggagtc 
      attgattcc cacttggtg atgcaccag ctgacttgt 
      aacggtgta cttgaaggt atccgtatt tgccggaaa 
      ggattcccc aacaggatg gtctacccc gacttcaaa 
      ctccggtga gcctatttt caatcctac tttagggaa 
      ttaagtccg atccttgat taaaaacct tgcgtagct 
      acatgatcc tagctcctg ccacaatgg cagccgaaa 
      aagacccca aggaagcgg cccggaaat gtctggaag 
      aggtcggct tagacccgg actcgtacc ggatcggac 
      ataccaagg caagggttg attgtaacc cctgtcact 
      tgtctttgc accttttgt tacatgtaa attaacctt 
      tgaccttta gttacaaga tcttgaatc ctgccgccg 
      tcgataagg aatctgatc caaagaaat gtgccgatc 
      ttatccttc aggccacag gtcttgacg ctgacctat 
      accgtttag gtcacacca aggcatgtt ttcctcttg 
      ttgttttgt tattgtttc tttgaccgg agtcggcag 
      actgacgtc cggattttt tcccgggtg tcacattta 
      gggacttga cgacaccca atactaacc aaacacttt 
      gcacctttg ttttcgtta ttcactagt ttagttctt 
      gcgtcaaac agatacaaa attctggcc gcaagcgct 
      attagggat gatatggca ccagaaaaa gcatcgcaa 
      gttattctg gaaacgatc aaccttgat gctgaacag 
      taccgactt ggtaaaact aaggtgttc ttccgcgct 
      ggtgtgttg ggtcagatg gaagagttg cgtgatgag 
      cgtcttggc aagatcgtc acctggatg caatcttgg 
      gtgcgtggt tacttgtcc aggaaggag ttcaagagg 
      ttgcaggag caacgtttg gccctccaa gtgtgccag 
      aggaacctc cgcaaatac ctcaaactc cgcacttgg 
      ccttggtac aagttgtgg cagaaagtc aagcctctc 
      ctcaacgtc actcgcatc gaggatgag attgcgaaa 
      ttggaggaa aaagcagcc aaagcacaa gaagcctat 
      gaacgggaa gccaaagcc aagaaggaa ctggaagga 
      ctttactcc aaacttttg gccgagaag accgaactc 
      ttgtccagt cttgaggga gagaaggga tccctttcc 
      gaagtacaa gaaagggcc aacaaactc caagcccag 
      aagagcgat cttgagtca caattatct gaaactcaa 
      gaccgcctc agccaggag gaggatgct cgcaaccag 
      ttgatgcaa cagaagaag aagcttgag caagaaatc 
      tccggttac aagaaagac atcgaagat ctcgaactc 
      aacctccag aaatccgag caagacaag gccaccaaa 
      gaccaccag atccgcaac ttgaacgac gaaatcgcc 
      caccaagat gagctcatc aacaagctc aacaaagaa 
      aagaaactt tcgggcgag aacagccaa agggtgtct 
      gaggaactc caagccgcc gaagacaaa gtcaaccac 
      ttgaacaaa gttaaggcc aaactcgaa caaaccctc 
      gacgagctc gaagactcc ctcgaacgc gagaagaaa 
      ctccgcggg gacgtcgaa aagtccaaa cgcaaggtt 
      gagggcgac ctcaaattg acccaggaa gccgtcgcc 
      gacctcgaa cgcaacaag aaggaactc gaacagacc 
      atccaacgc aaagacaag gaaatctcc tccttgacc 
      gccaaactt gaagacgag caatccgtc gtcggcaag 
      ctccagaaa cagatcaag gagctccaa gccaggatt 
      gaggaattg gaagaggaa gtcgaagcc gaacgccaa 
      gcccgcgcc aaggccgag aaacagaga gccgatttg 
      gccagggaa ttggaagag ttgggcgaa cgtttggag 
      gaagctggc ggtgccact tccgcccag attgaactc 
      aacaagaag cgcgaagcc gaacttgcg aaattgagg 
      agggatttg gaagaatca aacatccaa catgagtcg 
      actttggcc aacttgagg aagaagcac aacgatgcc 
      gtgtctgaa atgggtgaa caaatcgac cagttgaac 
      aaactcaag gcaaaggct gaaaaagaa aaggctgca 
      tactttgga gaactgaac gaccttcgt gcctccgtc 
      gaccatttg gccaacgaa aaggccgct gttgagaaa 
      gtctccaaa caactccaa caacaactc aacgatgtc 
      caaggcaaa ctcgacgag accaaccgc actcttaat 
      gacttcgac gccgccaag aagaagctt tcaatcgaa 
      aactccgat cttttgcgc caattggaa gaagccgag 
      agccaggtg tcccaattg agcaaaatc aaagtctca 
      ctcaccacc caattggaa gataccaag agactcgcc 
      gatgaagaa ggtcgcgaa agggccact cttttgggc 
      aaattccgc aatttggag cacgatttg gacaacatc 
      cgcgaacag gttgaagaa gaagctgaa gctaaagcc 
      gacattcaa cgccagttg agcaaagcc aatgccgaa 
      gcccaattg tggagacag aaatacgaa tccgaaggt 
      gtcgccaag tctgaggaa cttgaagag gccaagagg 
      aaactccaa gccaggctt gctgaagcc gaggaaacc 
      atcgaatcc ctcaaccag aaggttgta gctttggaa 
      aagaccaaa cagaggctc gccaccgaa gtcgaagat 
      ttgcaaatc gaagtcgac cgtgctaac gccatcgcc 
      aacgctgct gagaagaaa cagaaagcg ttcgacaag 
      atcatcggc gaatggaaa ctcaaagtc gatgatttg 
      gccgctgaa ctcgacgcc agccagaag gaatgccgc 
      aactactcc accgagctc ttccgtctc aagggagcc 
      tatgaagaa ggccaagaa cagcttgaa gccgtccgt 
      cgcgaaaac aagaacttg gccgatgaa gttaaggac 
      ctccttgac caaatcggt gaaggcggc cgcaacatc 
      cacgagatc gaaaaggcc cgcaaacgg ctcgaagcc 
      gaaaaagac gaactccaa gccgctttg gaagaagcc 
      gaagccgct ttggaacaa gaagagaac aaagttttg 
      cgttcgcaa ctcgagttg tctcaagtt cgtcaagaa 
      atcgaccgc aggatccaa gagaaggaa gaagaattc 
      gaaaacacc cgcaagaac caccaacgc gccctcgac 
      tccatgcaa gcctccctc gaggccgaa gccaaaggc 
      aaggctgag gcgctccgc atgaagaag aagttggaa 
      gccgacatc aacgagctc gagattgct ctcgaccac 
      gccaacaag gctaacgcc gaggcccag aagaccatc 
      aaacgctac caacagcaa ctcaaggac acccaaacc 
      gcccttgag gaggagcaa cgtgctcgt gacgaagcc 
      cgcgaacag ctcggaatc tccgaacgc cgagccaac 
      gctctccag aacgagctc gaagagagc aggactctc 
      ctcgagcaa gccgaccgt gctcgccgc caagccgag 
      caagaactg ggcgatgct cacgagcaa ctcaacgac 
      ttgtcggcc caaaactcc tccctttcc gccgccaag 
      aggaaactc gagaccgag ctccagact cttcactcc 
      gatcttgac gagcttttg aacgaggct aagaactct 
      gaagagaag gccaagaag gccatggtc gacgctgct 
      cgccttgct gacgagctc cgcgccgag caagaccac 
      gcccaaacc caggagaag ctccgcaag gcccttgag 
      acccaaatc aaggacttg caagtccgt ttggacgaa 
      gccgaagcc aatgctctc aaaggcggc aagaaactc 
      atccagaag ttggaacaa cgcgtccgc gaattggag 
      aacgagctc gatggcgag caacgaaga cacgccgat 
      gctcagaag aacttgagg aagtccgaa cgccgcatt 
      aaggaattg agcttccaa gcggaagaa gaccgcaag 
      aaccacgaa cgcatgcag gatttggtt gacaaattg 
      cagcagaag atcaagacc tacaagagg cagattgag 
      gaagctgag gaaatcgca gctctcaat ttggccaag 
      ttcaggaag gctcaacag gagttggaa gaagccgaa 
      gaacgtgct gatcttgcc gaacaagcc attgctaaa 
      ttccgcgcg aagggacgt ggaggatct gtagccaga 
      ggaggcagc ccagcgccc ccgagacag cgcccccag 
      atggacggc ctcaccttc cccccaagg ttcgacctg 
      gctcctgac gagatctaa gcgctgtgt taacgaact 
      tttcggcgg ataaaacca ccacaatcc gcctttaaa 
      cacacattc atttttttt cttttattt tttattttt 
      tgtttaggg ttgtatttt gaatgattc cgttgccca 
      ttatgcata cgagatagg ggaaataag ggtaaaaag 
      agacacgta cttccatcc aaacagccc ttacagtct 
      ccgagttct cattgccat cttatattt tatattagg 
      ttctaaaat tgtattgta tgtgtgaca tgtatggct 
      taaggcacc aataaacaa gttatatcg ttacaaaat 
      ctttttttt ccatacaaa accgtctgt aattatttc 
      aatgtcatt gttatttat ttattgtac aataaattc 
      tattgaaaa aa
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