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Gene information
General information
TC005903 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid52: 5491027..5496483
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005903
    iBeetle screen iB_00958
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    pupa eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    egg without cuticle embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 30.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 30.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005903 RA
      MSLNLFIRA PVSAGVKKS LKHQLQDYR LCHKLLNFS 
      SCFCTKTAP NNTQGEEKH EQIARNESP TPKFRRKLL 
      NGPNLKDFF RDENLKNQE NLQEEHVVP YLQDINRYG 
      QKRKVYFDV YGCQMNVND TEIIWSILQ KHNYLKTSN 
      LLEADVVLI VTCAIREGA ESKIWGRLE YLRGINKSK 
      ATFSSRFKV GVLGCMAER LKHKVLEKN KTVDLVAGP 
      DAYRDLPRL LALTENDQK SVNVLLSFD ETYADIMPV 
      RLNENSVSA FVSIMRGCD NMCTYCIVP FTRGKERSR 
      PVDSILREI ELLSQQGVK EVTLLGQNV NSYRDLSNS 
      GDIFINNQT NLAKGFKTV YKSKIGGRR FAHLLEKVA 
      DVNPEMRIR FTSPHPKDF PDEVLHVIK SRPNICKNL 
      HMPAQSGNT NVLERMRRG YTREAYIEL VDHIRYLLP 
      EVSLSSDFI CGFCGETEE EFEDTISLM EMVQYNNAY 
      LFPYSMREK TAAHRRYTD DVPAEVKQK RLERMIQTF 
      RFYAEKLNR AQIGRLQLI LIEGFSRRS RNQLAGRND 
      QNIKVVIPA GEVPQKDGR SSKELGPGD YVVVQINAA 
      NSQILKGIP LYHTTLSEH FLEYPDCFE NSCYSMM
    CDS sequence
    • >TC005903 RA
      atgagtttg aatttgttc atccgagcc ccagttagt 
      gccggtgtg aaaaaaagt ctgaaacac caacttcaa 
      gattatagg ttatgtcac aaactcctt aatttctcg 
      tcttgtttt tgtacaaaa accgctcca aataacaca 
      caaggtgaa gaaaagcat gagcaaatt gcacgaaac 
      gagtcacca actccaaaa tttcgtcgt aaattatta 
      aacggccct aacctcaaa gacttcttt cgggatgaa 
      aatttgaaa aatcaggaa aacctccag gaggagcac 
      gtagttccc tatttacag gacatcaat agatacggc 
      caaaaacgc aaagtttat tttgacgtc tatgggtgt 
      cagatgaat gtcaacgac accgaaata atttggtca 
      attttgcaa aagcacaac tatttgaag actagtaat 
      ttattggag gccgatgtc gtgcttatt gtcacttgc 
      gcgatacgc gaaggggcc gagtcgaaa atttggggc 
      cgtttggag tacctgcga gggattaat aagagcaaa 
      gcgacgttc agttcacgg tttaaggtc ggggtcttg 
      gggtgcatg gccgaacgg ttgaaacac aaagtcttg 
      gagaagaac aagacggtt gatttggtc gcggggcct 
      gacgcctat agggatcta ccaagactg ctggctttg 
      accgaaaat gaccaaaag tcggtcaat gttttactg 
      tcgtttgat gagacttac gccgatatc atgccggtg 
      agactcaac gagaactcc gtctcggct tttgtttcg 
      ataatgcgc ggttgtgac aacatgtgc acttactgc 
      atcgtgccc ttcacccgg ggcaaagaa cgttcccgt 
      cctgtggac tcaatctta cgcgaaatt gaattatta 
      tcgcaacaa ggcgtcaaa gaagtgact ctcttgggc 
      cagaacgta aacagttat agagactta tcaaactct 
      ggcgatatt tttattaac aatcagacg aacctggcc 
      aaaggtttc aaaaccgtc tacaagtcg aaaatcggc 
      ggtcgccgc ttcgcccac ttactagaa aaagtcgca 
      gacgtgaac cccgaaatg aggattcgg ttcacatcg 
      ccccacccg aaggacttc cccgatgaa gtcctccac 
      gtgataaaa tcgcgcccc aacatttgc aaaaacctc 
      cacatgccg gcgcagtcg ggcaacacc aatgtgctg 
      gagcggatg agaaggggg tacacgagg gaggcctac 
      atcgaattg gtcgaccac attcggtat ttgctgcca 
      gaagtcagc ttaagctcg gattttatc tgcggattt 
      tgcggcgaa acggaggaa gagttcgag gatacgatc 
      tcgttaatg gaaatggtc caatacaac aacgcgtat 
      ttattccca tacagcatg cgcgaaaaa acagctgcg 
      catcggcga tacactgat gatgtcccg gctgaagtc 
      aagcagaaa cgtctcgaa aggatgatt cagacgttt 
      aggttttac gccgagaag ctcaatagg gcccaaatc 
      gggcgctta caattgatt ttaattgaa ggttttagt 
      agaagatcg aggaatcaa ttggctgga cgaaacgat 
      caaaatatt aaagtcgtg atccctgct ggtgaagtg 
      ccgcagaaa gacggccga agtagcaaa gaactagga 
      cctggggat tacgtggtg gtgcaaata aacgctgca 
      aattcgcaa attctcaaa ggaataccg ttgtaccac 
      acaacttta agtgaacat tttctcgaa tatcccgat 
      tgtttcgag aattcttgt tatagcatg atgtaa
    mRNA sequence
    • >TC005903 RA
      cacgcgacc tctggtgtc aaaatatta ttttcaaaa 
      tgttttttt taataaatt tgtaatttt cgtgatgta 
      aatacgttc aaaatgagt ttgaatttg ttcatccga 
      gccccagtt agtgccggt gtgaaaaaa agtctgaaa 
      caccaactt caagattat aggttatgt cacaaactc 
      cttaatttc tcgtcttgt ttttgtaca aaaaccgct 
      ccaaataac acacaaggt gaagaaaag catgagcaa 
      attgcacga aacgagtca ccaactcca aaatttcgt 
      cgtaaatta ttaaacggc cctaacctc aaagacttc 
      tttcgggat gaaaatttg aaaaatcag gaaaacctc 
      caggaggag cacgtagtt ccctattta caggacatc 
      aatagatac ggccaaaaa cgcaaagtt tattttgac 
      gtctatggg tgtcagatg aatgtcaac gacaccgaa 
      ataatttgg tcaattttg caaaagcac aactatttg 
      aagactagt aatttattg gaggccgat gtcgtgctt 
      attgtcact tgcgcgata cgcgaaggg gccgagtcg 
      aaaatttgg ggccgtttg gagtacctg cgagggatt 
      aataagagc aaagcgacg ttcagttca cggtttaag 
      gtcggggtc ttggggtgc atggccgaa cggttgaaa 
      cacaaagtc ttggagaag aacaagacg gttgatttg 
      gtcgcgggg cctgacgcc tatagggat ctaccaaga 
      ctgctggct ttgaccgaa aatgaccaa aagtcggtc 
      aatgtttta ctgtcgttt gatgagact tacgccgat 
      atcatgccg gtgagactc aacgagaac tccgtctcg 
      gcttttgtt tcgataatg cgcggttgt gacaacatg 
      tgcacttac tgcatcgtg cccttcacc cggggcaaa 
      gaacgttcc cgtcctgtg gactcaatc ttacgcgaa 
      attgaatta ttatcgcaa caaggcgtc aaagaagtg 
      actctcttg ggccagaac gtaaacagt tatagagac 
      ttatcaaac tctggcgat atttttatt aacaatcag 
      acgaacctg gccaaaggt ttcaaaacc gtctacaag 
      tcgaaaatc ggcggtcgc cgcttcgcc cacttacta 
      gaaaaagtc gcagacgtg aaccccgaa atgaggatt 
      cggttcaca tcgccccac ccgaaggac ttccccgat 
      gaagtcctc cacgtgata aaatcgcgc cccaacatt 
      tgcaaaaac ctccacatg ccggcgcag tcgggcaac 
      accaatgtg ctggagcgg atgagaagg gggtacacg 
      agggaggcc tacatcgaa ttggtcgac cacattcgg 
      tatttgctg ccagaagtc agcttaagc tcggatttt 
      atctgcgga ttttgcggc gaaacggag gaagagttc 
      gaggatacg atctcgtta atggaaatg gtccaatac 
      aacaacgcg tatttattc ccatacagc atgcgcgaa 
      aaaacagct gcgcatcgg cgatacact gatgatgtc 
      ccggctgaa gtcaagcag aaacgtctc gaaaggatg 
      attcagacg tttaggttt tacgccgag aagctcaat 
      agggcccaa atcgggcgc ttacaattg attttaatt 
      gaaggtttt agtagaaga tcgaggaat caattggct 
      ggacgaaac gatcaaaat attaaagtc gtgatccct 
      gctggtgaa gtgccgcag aaagacggc cgaagtagc 
      aaagaacta ggacctggg gattacgtg gtggtgcaa 
      ataaacgct gcaaattcg caaattctc aaaggaata 
      ccgttgtac cacacaact ttaagtgaa cattttctc 
      gaatatccc gattgtttc gagaattct tgttatagc 
      atgatgtaa ataaaatta ttgaacgaa aattcaatc 
      gttttattc ttttggcat aacaactgg aagcaagta 
      aataaaaaa ataaatgga ag